##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937637_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2401855 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22001786119478 33.0 31.0 34.0 31.0 34.0 2 32.4264345682816 34.0 31.0 34.0 31.0 34.0 3 32.48981558004126 34.0 31.0 34.0 31.0 34.0 4 36.100787099970645 37.0 35.0 37.0 35.0 37.0 5 36.02111617895335 37.0 35.0 37.0 35.0 37.0 6 36.071246182638 37.0 35.0 37.0 35.0 37.0 7 36.0269383455704 37.0 35.0 37.0 35.0 37.0 8 35.994045852060175 37.0 35.0 37.0 35.0 37.0 9 37.678483921802105 39.0 37.0 39.0 35.0 39.0 10-11 37.59911068736456 39.0 37.0 39.0 35.0 39.0 12-13 37.49150073588955 39.0 37.0 39.0 35.0 39.0 14-15 38.69615401429312 40.0 38.0 41.0 34.5 41.0 16-17 38.6634055761068 40.0 38.0 41.0 34.5 41.0 18-19 38.651610109686054 40.0 38.0 41.0 34.0 41.0 20-21 38.55844607605371 40.0 38.0 41.0 34.0 41.0 22-23 38.50869681974973 40.0 38.0 41.0 34.0 41.0 24-25 38.52840783477771 40.0 38.0 41.0 34.0 41.0 26-27 38.35079823719583 40.0 38.0 41.0 34.0 41.0 28-29 38.31203653009861 40.0 38.0 41.0 34.0 41.0 30-31 38.31754331547908 40.0 38.0 41.0 34.0 41.0 32-33 38.17745263556709 40.0 37.5 41.0 34.0 41.0 34-35 38.02408804861243 40.0 37.0 41.0 33.5 41.0 36-37 37.85243988500555 40.0 37.0 41.0 33.0 41.0 38-39 37.70962110535399 40.0 37.0 41.0 33.0 41.0 40-41 37.531717776468604 40.0 37.0 41.0 33.0 41.0 42-43 37.29279702563227 40.0 37.0 41.0 32.5 41.0 44-45 36.95952440925868 40.0 36.0 41.0 31.0 41.0 46-47 36.57777176390748 39.5 36.0 41.0 30.5 41.0 48-49 36.20559442597492 39.0 36.0 41.0 30.0 41.0 50-51 35.949882278488914 39.0 35.0 40.5 29.0 41.0 52-53 35.687187403069714 39.0 35.0 40.0 28.0 41.0 54-55 35.46274233873402 39.0 35.0 40.0 28.0 41.0 56-57 35.18842831894514 38.0 35.0 40.0 27.0 41.0 58-59 34.929114580189065 38.0 34.0 40.0 26.5 41.0 60-61 34.57931411346647 38.0 34.0 40.0 26.0 41.0 62-63 34.18243399372568 37.5 34.0 40.0 26.0 41.0 64-65 33.77838795431032 37.0 33.0 39.5 24.5 41.0 66-67 33.35160657075468 36.0 33.0 39.0 23.0 40.0 68-69 32.87821308946627 36.0 32.0 39.0 22.0 40.0 70-71 32.44388337347591 35.0 32.0 38.0 21.5 40.0 72-73 31.96298361058432 35.0 31.0 37.5 20.0 39.5 74-75 31.47898769909091 35.0 31.0 37.0 18.5 39.0 76-77 30.221098484296512 33.5 29.5 35.5 18.0 38.0 78-79 30.83201275680672 34.0 31.0 36.0 18.0 38.0 80-81 30.650479733372748 34.0 31.0 36.0 17.5 37.0 82-83 30.257377943297993 34.0 30.0 35.0 12.0 37.0 84-85 29.84256814004176 34.0 30.0 35.0 7.5 36.5 86-87 29.488886922815908 34.0 29.5 35.0 4.5 36.0 88-89 29.076166338101174 34.0 29.0 35.0 2.0 36.0 90-91 28.55655982563477 34.0 29.0 35.0 2.0 35.0 92-93 28.080242354346954 33.0 28.0 35.0 2.0 35.0 94-95 27.5648681956238 33.0 27.0 35.0 2.0 35.0 96-97 27.019233051120906 33.0 26.0 35.0 2.0 35.0 98-99 26.162863703262687 32.5 25.0 34.0 2.0 35.0 100-101 23.666973027097804 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3359888001827116 1101 2 0.2737232453099452 1101 3 0.26955383394835764 1101 4 0.14816814035516757 1101 5 0.20248142440055972 1101 6 0.13047852474349497 1101 7 0.17045475047419956 1101 8 0.21486974978329698 1101 9 0.25786430459770315 1101 10-11 0.29472350205423226 1101 12-13 0.28191718610370486 1101 14-15 0.2726132276925668 1101 16-17 0.40468550599123887 1101 18-19 0.4033469674838983 1101 20-21 0.3452789739099771 1101 22-23 0.3428566757145646 1101 24-25 0.35640565107748756 1101 26-27 0.2620417889589106 1101 28-29 0.4750838293324122 1101 30-31 0.4226841223297342 1101 32-33 0.4267326211551534 1101 34-35 0.38555591669697264 1101 36-37 0.31728588413538716 1101 38-39 0.3123098378761995 1101 40-41 0.38743390070261086 1101 42-43 0.46250762869624396 1101 44-45 0.4560657020368595 1101 46-47 0.46084362131887957 1101 48-49 0.41809844788846107 1101 50-51 0.3135857092115941 1101 52-53 0.2601317987431955 1101 54-55 0.3161750430720076 1101 56-57 0.3047419702931151 1101 58-59 0.2629539356918045 1101 60-61 0.24266835370754336 1101 62-63 0.253211553750198 1101 64-65 0.35130422927037586 1101 66-67 0.3032756094300808 1101 68-69 0.33513386522671595 1101 70-71 0.3661692821540541 1101 72-73 0.2686283575252624 1101 74-75 0.26319703499730096 1101 76-77 0.21411419883569494 1101 78-79 0.28094173267909284 1101 80-81 0.5502553938291612 1101 82-83 0.5566977887865825 1101 84-85 0.669548377278506 1101 86-87 0.5167027218767046 1101 88-89 0.5475936617014874 1101 90-91 0.6290491011798203 1101 92-93 0.8532322988286936 1101 94-95 0.8897116056423862 1101 96-97 0.9022191196093701 1101 98-99 1.1941275219066405 1101 100-101 1.820190318496266 1102 1 0.2515288457034188 1102 2 0.1902907162873575 1102 3 0.19282695677750894 1102 4 0.09225037904720779 1102 5 0.08124068518451821 1102 6 0.14528356858264146 1102 7 0.1785056493755377 1102 8 0.12104295452738256 1102 9 0.22753414692191143 1102 10-11 0.21849655720237848 1102 12-13 0.2549658452524355 1102 14-15 0.2622175659747583 1102 16-17 0.32145427560651285 1102 18-19 0.3048020391629578 1102 20-21 0.29089607110686444 1102 22-23 0.2617847831445488 1102 24-25 0.3044594386317172 1102 26-27 0.19747237267612405 1102 28-29 0.40192852028143733 1102 30-31 0.3818358491720204 1102 32-33 0.4433858097674417 1102 34-35 0.40087728979789006 1102 36-37 0.3619215022024491 1102 38-39 0.3191037541220041 1102 40-41 0.4292047635446252 1102 42-43 0.45033725957664217 1102 44-45 0.6022360739474308 1102 46-47 0.5237245013672194 1102 48-49 0.40627851981173535 1102 50-51 0.2555644294685848 1102 52-53 0.5257973332067749 1102 54-55 0.4485215711763644 1102 56-57 0.4045608015190396 1102 58-59 0.413491999624533 1102 60-61 0.3229029555827907 1102 62-63 0.4437068739929586 1102 64-65 0.5285487265261537 1102 66-67 0.5716508259046194 1102 68-69 0.4969671550674448 1102 70-71 0.33150980827961973 1102 72-73 0.45587185003220654 1102 74-75 0.5075917226669411 1102 76-77 0.5237701142246785 1102 78-79 0.3575015005821136 1102 80-81 0.4710157723961963 1102 82-83 0.6123429073120583 1102 84-85 0.577940925670422 1102 86-87 0.48193283029189615 1102 88-89 0.4922157784560923 1102 90-91 0.5235492525898913 1102 92-93 0.5876888692581019 1102 94-95 0.7780997094161961 1102 96-97 0.8727246827082951 1102 98-99 1.0358469611451575 1102 100-101 1.4895295705444909 1103 1 0.2406000925142706 1103 2 0.27855606900810415 1103 3 0.2428270901098628 1103 4 0.1743547769482916 1103 5 0.18114211924066836 1103 6 0.23601740320118836 1103 7 0.2365247239023276 1103 8 0.1829728337082699 1103 9 0.32253611690746453 1103 10-11 0.2828614185267284 1103 12-13 0.30571563975393445 1103 14-15 0.32952707237171097 1103 16-17 0.2780659271210695 1103 18-19 0.35658399276196207 1103 20-21 0.3643219493170946 1103 22-23 0.38409613619462135 1103 24-25 0.3279979326827558 1103 26-27 0.32117313220388866 1103 28-29 0.34165487895480595 1103 30-31 0.41060547152812177 1103 32-33 0.337758167703484 1103 34-35 0.2733935580119251 1103 36-37 0.27303432070844735 1103 38-39 0.2976689113108577 1103 40-41 0.3375536856525301 1103 42-43 0.37500431201826245 1103 44-45 0.41761426117405875 1103 46-47 0.40701669055783185 1103 48-49 0.480650557750117 1103 50-51 0.5164845993873399 1103 52-53 0.39177489937129195 1103 54-55 0.3079676852381965 1103 56-57 0.4084291898175252 1103 58-59 0.3191864411986316 1103 60-61 0.3954463402646411 1103 62-63 0.21000900444399662 1103 64-65 0.4365976508340452 1103 66-67 0.445408501681662 1103 68-69 0.5408453332941434 1103 70-71 0.4693770472531966 1103 72-73 0.5255870887871232 1103 74-75 0.6264878507620004 1103 76-77 0.46783202443733884 1103 78-79 0.6008195495830861 1103 80-81 0.6823193061702781 1103 82-83 0.6249362316276823 1103 84-85 0.6486769902726088 1103 86-87 0.6964237573584313 1103 88-89 0.6445859110751186 1103 90-91 0.6712589193856644 1103 92-93 0.8902602337347609 1103 94-95 1.0336265022197146 1103 96-97 1.151235390296442 1103 98-99 1.2109819268220754 1103 100-101 1.486007263041408 1104 1 0.26028340905565983 1104 2 0.21693433421294372 1104 3 0.25506082882325387 1104 4 0.11958636765780994 1104 5 0.11199278014117908 1104 6 0.14658882034731135 1104 7 0.19121003499551392 1104 8 0.14899604203343841 1104 9 0.14820806304059175 1104 10-11 0.3017860901409293 1104 12-13 0.2928771958489662 1104 14-15 0.3237295426249247 1104 16-17 0.3750039893790458 1104 18-19 0.33566541956711404 1104 20-21 0.3098910045367589 1104 22-23 0.3126722440324201 1104 24-25 0.12831631321723336 1104 26-27 0.27924349192869613 1104 28-29 0.2867618711349209 1104 30-31 0.3010280141574029 1104 32-33 0.35624914215338777 1104 34-35 0.2666741779582935 1104 36-37 0.23649826060984935 1104 38-39 0.315083526775247 1104 40-41 0.4530115759011366 1104 42-43 0.5788625424623035 1104 44-45 0.5239316748304432 1104 46-47 0.48189544879246426 1104 48-49 0.5160414980309369 1104 50-51 0.59404950034736 1104 52-53 0.6121202868198807 1104 54-55 0.5467309785927057 1104 56-57 0.5896134139043667 1104 58-59 0.541572627391254 1104 60-61 0.6986112241888804 1104 62-63 0.5574887817694432 1104 64-65 0.7182675409695207 1104 66-67 0.6258057962436325 1104 68-69 0.5541720928968417 1104 70-71 0.7255519488016731 1104 72-73 0.7154360717932811 1104 74-75 0.8416245645273968 1104 76-77 0.7107702219412069 1104 78-79 0.7627716289724269 1104 80-81 0.6846431134461817 1104 82-83 0.6157173221712284 1104 84-85 0.69670374945267 1104 86-87 0.8318710555128774 1104 88-89 0.6867915700103389 1104 90-91 0.7329665645823731 1104 92-93 0.86789475706939 1104 94-95 0.9976707640388547 1104 96-97 1.240268557725976 1104 98-99 1.3128615846369591 1104 100-101 1.5965608641421056 1105 1 0.10528967957512236 1105 2 0.1136973014827376 1105 3 0.14294816521171327 1105 4 0.11546546417414305 1105 5 0.05879381554276364 1105 6 0.1294436496792173 1105 7 0.125437945240499 1105 8 0.1349759731464033 1105 9 0.1819689010103005 1105 10-11 0.19843070921614014 1105 12-13 0.16434799302885494 1105 14-15 0.10023171820011356 1105 16-17 0.24099779657596798 1105 18-19 0.26117361542144835 1105 20-21 0.2862266864529275 1105 22-23 0.2837192611580761 1105 24-25 0.23948331993341299 1105 26-27 0.23027039631077173 1105 28-29 0.1599215486473824 1105 30-31 0.209481654718779 1105 32-33 0.18068314271901187 1105 34-35 0.29157776610141184 1105 36-37 0.2370819397166244 1105 38-39 0.22946175212633335 1105 40-41 0.271649255966949 1105 42-43 0.24706168925888505 1105 44-45 0.22254767815064014 1105 46-47 0.2996677681494262 1105 48-49 0.32968138543568415 1105 50-51 0.2507346899576177 1105 52-53 0.3544794113528198 1105 54-55 0.3991928628959016 1105 56-57 0.3081287632137375 1105 58-59 0.43408263781041256 1105 60-61 0.5028620204593892 1105 62-63 0.44069757539501353 1105 64-65 0.4628871321426118 1105 66-67 0.5464156828647688 1105 68-69 0.39558502845125787 1105 70-71 0.3310706508799157 1105 72-73 0.327465053234544 1105 74-75 0.3568756803702833 1105 76-77 0.3517561365712041 1105 78-79 0.4378834228698487 1105 80-81 0.4983042841774825 1105 82-83 0.30328429612398367 1105 84-85 0.40109720850596986 1105 86-87 0.4849413849755493 1105 88-89 0.5311095184008501 1105 90-91 0.6292092996317216 1105 92-93 0.616194590124266 1105 94-95 0.6549451954434993 1105 96-97 0.588352123160437 1105 98-99 0.7242678030445227 1105 100-101 1.0646642520929674 1106 1 -0.26867249716867647 1106 2 -0.221687550349408 1106 3 -0.25846021880865777 1106 4 -0.17275066892653967 1106 5 -0.19785267828356012 1106 6 -0.2709961539314989 1106 7 -0.1732621284364413 1106 8 -0.16533749072221582 1106 9 -0.394192767242437 1106 10-11 -0.4188184168034681 1106 12-13 -0.5949665128057475 1106 14-15 -0.4652183867066668 1106 16-17 -0.2961298444399745 1106 18-19 -0.2841863217328111 1106 20-21 -0.2869901805035653 1106 22-23 -0.34150640405581356 1106 24-25 -0.37938556971543136 1106 26-27 -0.40389856752487674 1106 28-29 -0.5393697433066791 1106 30-31 -0.4307666580502101 1106 32-33 -0.6631200185788586 1106 34-35 -0.8980722447698497 1106 36-37 -0.5253711199158104 1106 38-39 -0.5801720447197951 1106 40-41 -0.6892644546083275 1106 42-43 -0.9066058962753729 1106 44-45 -0.9254300665338206 1106 46-47 -0.728610354825868 1106 48-49 -0.689824520191543 1106 50-51 -0.8996831708181006 1106 52-53 -0.8761213015252807 1106 54-55 -0.895898115193674 1106 56-57 -1.1529141460456316 1106 58-59 -1.1445087669352816 1106 60-61 -1.346226632600164 1106 62-63 -1.279569245836612 1106 64-65 -1.6980431675801242 1106 66-67 -1.5684015214832954 1106 68-69 -1.4920177614390937 1106 70-71 -1.421399784564258 1106 72-73 -1.484104720792498 1106 74-75 -1.5868669879003043 1106 76-77 -1.4840460843714744 1106 78-79 -1.4627380237110934 1106 80-81 -1.5398554895254222 1106 82-83 -1.6272900307226443 1106 84-85 -1.3798218848925714 1106 86-87 -1.2578963280019728 1106 88-89 -1.4160020579105037 1106 90-91 -1.5907189099676948 1106 92-93 -1.6003954860328484 1106 94-95 -1.524601278024246 1106 96-97 -1.5562466980813952 1106 98-99 -1.6307308476076976 1106 100-101 -0.9260960844391022 1107 1 0.23530818122261365 1107 2 0.18094986703113847 1107 3 0.17503893164546724 1107 4 0.16144715516853836 1107 5 0.09244915513428253 1107 6 0.10133302507804132 1107 7 0.08535671457993743 1107 8 0.11038186557998841 1107 9 0.16960963995245493 1107 10-11 0.0874423359564318 1107 12-13 0.21445205977926918 1107 14-15 0.18945998679105713 1107 16-17 0.17175162834341506 1107 18-19 0.25756543295747747 1107 20-21 0.18023167106164095 1107 22-23 0.30003434301080745 1107 24-25 0.25343332133809326 1107 26-27 0.28980580830354086 1107 28-29 0.30658125880162146 1107 30-31 0.311289598788413 1107 32-33 0.38804888537352866 1107 34-35 0.4163661208995961 1107 36-37 0.3536051550701842 1107 38-39 0.24575146946450843 1107 40-41 0.3190838680945589 1107 42-43 0.3638263491054943 1107 44-45 0.2943690670183514 1107 46-47 0.2973587946497247 1107 48-49 0.4139496874178903 1107 50-51 0.4465798058838786 1107 52-53 0.38786160080808685 1107 54-55 0.4009398839701248 1107 56-57 0.5484012712103805 1107 58-59 0.5072263526683187 1107 60-61 0.5621506903073268 1107 62-63 0.6498996767968919 1107 64-65 0.686632893017844 1107 66-67 0.7409809342824971 1107 68-69 0.7067491071615137 1107 70-71 0.5990850348071675 1107 72-73 0.493273607336814 1107 74-75 0.6378464349244588 1107 76-77 0.5388811571397376 1107 78-79 0.7368562402975165 1107 80-81 0.8681752542497598 1107 82-83 0.9093680070487871 1107 84-85 0.8991168499512661 1107 86-87 0.9837364902663772 1107 88-89 0.8719758450010815 1107 90-91 1.0617650554099747 1107 92-93 1.2213423023012417 1107 94-95 1.0813071133539793 1107 96-97 1.0210695375852978 1107 98-99 1.147567036089029 1107 100-101 1.7349310209668545 1108 1 0.09978386722537635 1108 2 0.05665137312480795 1108 3 0.05577870669058882 1108 4 0.08948022893854812 1108 5 0.008458692031034332 1108 6 -0.009242203263298165 1108 7 0.061076151292290604 1108 8 0.031539917328224476 1108 9 0.012099098862762503 1108 10-11 0.019163792799631096 1108 12-13 0.12596422167161592 1108 14-15 0.08448803763884882 1108 16-17 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2316 100-101 0.45491312761125613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 9.0 4 17.0 5 64.0 6 121.0 7 275.0 8 645.0 9 1212.0 10 2110.0 11 3496.0 12 5381.0 13 7843.0 14 10089.0 15 14285.0 16 19640.0 17 27426.0 18 25023.0 19 14673.0 20 12307.0 21 12391.0 22 12661.0 23 13907.0 24 15700.0 25 18959.0 26 23074.0 27 28585.0 28 35288.0 29 44454.0 30 55822.0 31 70742.0 32 90224.0 33 118886.0 34 163546.0 35 235423.0 36 358577.0 37 535782.0 38 394506.0 39 28702.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22238058862877 19.594385776678934 15.328738948945023 21.854494685747273 2 15.525458447741434 20.662113241640316 40.32857936886282 23.483848941755433 3 19.783708841707764 24.71531378871747 30.08220729394572 25.418770075629045 4 12.938124907623482 16.999111103709424 37.00414887659746 33.05861511206963 5 13.436769112835966 37.85583808256014 33.93394206158429 14.773450743019605 6 31.849133274073584 36.4515343349203 17.032918306891965 14.666414084114155 7 28.649564607355565 33.38719448093245 19.99900077231973 17.964240139392263 8 26.64732050852362 35.4823667540297 18.972460868786833 18.89785186865985 9 26.489900514394083 17.1061117344719 20.25084778223498 36.15313996889904 10-11 25.050742030638816 27.29765119043406 26.512549675146918 21.139057103780203 12-13 26.11787003613866 24.480773478523634 27.83311886911118 21.568237616226526 14-15 22.73929941649267 25.602877775719186 26.714060590668463 24.943762217119684 16-17 21.53508434106139 29.3367209927327 27.187569607657412 21.940625058548495 18-19 21.48158402568015 27.36942904546694 30.230238711329367 20.91874821752354 20-21 22.202598455898258 26.122869064027533 30.61567762676221 21.058854853312 22-23 21.96268696021279 26.15530883005328 30.55629400188899 21.325710207844946 24-25 21.888269691550903 26.336081903362192 30.69317256870211 21.082475836384795 26-27 21.680119992214244 26.506517446647287 30.671021692901952 21.142340868236516 28-29 21.28142099026654 26.818622393536895 30.833393704269017 21.066562911927548 30-31 21.46431018198646 26.469505126143154 30.71495379924138 21.351230892629008 32-33 21.37751862622848 26.557681458705872 30.683346829846098 21.381453085219547 34-35 21.904153248218563 26.50372316397118 30.51143803435262 21.08068555345764 36-37 21.830720274855143 26.269429318353748 30.5658520297088 21.333998377082303 38-39 21.577947045096394 26.60928740494326 30.62537080714697 21.187394742813368 40-41 21.76647167808148 26.653079917563545 30.441305660220248 21.139142744134727 42-43 21.651547150341273 26.62571498353125 30.181565371216763 21.54117249491071 44-45 21.749397861236417 26.6735710523741 29.963923717293504 21.613107369095967 46-47 21.941915727635514 27.072075541612627 29.47032606048242 21.515682670269438 48-49 22.031284153289853 27.22377079382394 29.170724294347494 21.57422075853871 50-51 21.962004367457652 27.29658951102377 29.059247956267136 21.68215816525144 52-53 22.183899982284522 27.639746704057217 28.596757214061054 21.579596099597207 54-55 21.940729144765193 27.399197703441715 28.70044195007609 21.959631201717006 56-57 22.040568643819046 27.646527371552406 28.68974188699984 21.623162097628708 58-59 22.293185891737842 27.356001923513283 28.640342568556388 21.710469616192483 60-61 22.06515380820241 27.34315768437312 29.19505965181079 21.39662885561368 62-63 22.008676627023696 27.366597900372835 29.112540099214986 21.512185373388483 64-65 21.902841761888205 27.469893061820965 28.9004956585639 21.726769517726925 66-67 21.866265865341582 27.526765770623125 28.685391083141987 21.92157728089331 68-69 21.79654891739926 27.48531863913517 28.853823398997857 21.864309044467714 70-71 21.9313405680193 27.572209812832167 28.63107889527053 21.865370723878 72-73 22.024643452664712 27.38516688143123 28.705916885074245 21.884272780829818 74-75 21.792843448084916 27.902267205972052 28.3282712736614 21.97661807228163 76-77 21.900385327174206 27.594359359744864 28.434522483663667 22.070732829417263 78-79 21.95365665287871 27.60172866388687 28.466685124622426 21.97792955861199 80-81 21.88065058048883 27.824556436587557 28.417660516559078 21.87713246636454 82-83 21.954435529786526 27.757963186286233 28.487088803857198 21.800512480070047 84-85 21.917304612151213 27.659188684060755 28.323509659841513 22.099997043946523 86-87 21.856356857512218 27.521852901195114 28.44842840221412 22.173361839078545 88-89 21.923492467280496 27.55174646262993 28.370904988019674 22.1538560820699 90-91 21.899309375356534 27.498736256003305 28.473276498858674 22.128677869781487 92-93 21.98989130835298 27.492714301743966 28.45651449364215 22.0608798962609 94-95 21.80156587304396 27.387456778198516 28.463958065745015 22.347019283012504 96-97 21.626409587589592 27.4395415210327 28.516001174092526 22.41804771728518 98-99 21.78042852607639 27.42494028009805 28.182735093444084 22.61189610038148 100-101 21.568412718010048 27.307290559513575 28.615959827168048 22.508336895308332 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 497.0 1 365.0 2 330.5 3 458.0 4 410.5 5 399.5 6 800.0 7 1906.0 8 3232.0 9 3952.5 10 3817.5 11 3464.5 12 3602.0 13 3991.0 14 4355.5 15 4627.0 16 4775.5 17 5039.5 18 5357.0 19 5623.0 20 6037.5 21 6641.0 22 7575.5 23 8929.5 24 10873.5 25 13231.0 26 15782.0 27 18636.5 28 21150.0 29 24141.0 30 28145.0 31 31921.0 32 36178.5 33 41326.0 34 47523.5 35 53026.5 36 58170.0 37 63331.5 38 67024.0 39 69738.0 40 72819.0 41 77461.0 42 81780.0 43 86134.0 44 90622.5 45 93518.0 46 93989.0 47 98009.0 48 106302.5 49 103938.0 50 94771.5 51 87772.5 52 80844.5 53 76644.5 54 73111.5 55 66866.5 56 59373.0 57 51671.5 58 44871.5 59 39092.0 60 33043.0 61 26476.0 62 20176.0 63 15109.0 64 11262.0 65 8542.0 66 6271.5 67 4662.0 68 3239.0 69 2002.5 70 1368.5 71 1016.0 72 809.0 73 660.5 74 493.5 75 387.5 76 270.5 77 139.0 78 73.0 79 48.0 80 30.5 81 19.5 82 10.5 83 4.5 84 4.0 85 4.5 86 1.5 87 0.0 88 0.0 89 1.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03967766580413888 2 0.0 3 0.0 4 0.0 5 0.009825738856009208 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005558203971513684 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.2898967672902817E-4 22-23 0.0016861967104592074 24-25 0.0 26-27 0.0013531208170351665 28-29 0.0015404760070861895 30-31 2.081724333900256E-5 32-33 0.0 34-35 0.0 36-37 3.3307589342404097E-4 38-39 0.0 40-41 2.0817243339002562E-4 42-43 0.0203384467422055 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.081724333900256E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.2898967672902817E-4 84-85 1.6653794671202049E-4 86-87 0.0 88-89 0.0 90-91 0.010866601022959338 92-93 0.0025188864440193097 94-95 0.0 96-97 0.0 98-99 7.286035168650898E-4 100-101 0.0138018323337587 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2401855.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.797911665763195 #Duplication Level Percentage of deduplicated Percentage of total 1 74.15593916464762 37.66966847177479 2 14.06672859713503 14.291208734070606 3 4.7594723614477354 7.253137697773902 4 2.228636846734126 4.528403907018606 5 1.196439106490776 3.0388304022491543 6 0.7630754220129567 2.325758272903749 7 0.48770349645592415 1.734202339243632 8 0.3687076024285318 1.4983660974927915 9 0.2750514798627931 1.2574836699846847 >10 1.5249867999797282 14.206761248993526 >50 0.11225384462381169 3.9352955322751515 >100 0.05739213940265355 5.106416658460626 >500 0.0022992701316997817 0.8094944060767418 >1k 0.0010675182754317145 0.9244988416919878 >5k 8.21167904178242E-5 0.3352523210160405 >10k+ 1.642335808356484E-4 1.0852213989739095 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14196 0.5910431728809608 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11820 0.4921196325340205 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8037 0.33461636943112716 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4635 0.19297584575255375 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2957 0.12311317710686116 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.163448667800513E-5 0.0 18-19 0.0 2.0817243339002564E-5 0.0 4.163448667800513E-5 0.0 20-21 0.0 4.163448667800513E-5 0.0 6.245173001700769E-5 0.0 22-23 0.0 4.163448667800513E-5 0.0 1.665379467120205E-4 0.0 24-25 0.0 4.163448667800513E-5 0.0 4.579793534580564E-4 0.0 26-27 0.0 4.163448667800513E-5 0.0 6.453345435090794E-4 0.0 28-29 0.0 4.163448667800513E-5 0.0 0.0011033138969671358 0.0 30-31 0.0 4.163448667800513E-5 0.0 0.002810327850765346 0.0 32-33 0.0 4.163448667800513E-5 0.0 0.004683879751275577 0.0 34-35 0.0 4.163448667800513E-5 0.0 0.006932142031887854 0.0 36-37 0.0 4.163448667800513E-5 0.0 0.009326125015873148 0.0 38-39 0.0 4.163448667800513E-5 0.0 0.012302990813350515 0.0 40-41 0.0 8.326897335601026E-5 0.0 0.016362353264456016 0.0 42-43 0.0 8.326897335601026E-5 0.0 0.02104623301573159 0.0 44-45 0.0 8.326897335601026E-5 0.0 0.028186547481009468 0.0 46-47 0.0 8.326897335601026E-5 0.0 0.0438619317152784 0.0 48-49 0.0 8.326897335601026E-5 0.0 0.07231910335969491 0.0 50-51 0.0 8.326897335601026E-5 0.0 0.09680018152636191 0.0 52-53 0.0 8.326897335601026E-5 0.0 0.1202195802827398 0.0 54-55 0.0 1.2490346003401538E-4 0.0 0.14440921704266077 0.0 56-57 0.0 1.2490346003401538E-4 0.0 0.16776616406902164 0.0 58-59 0.0 1.2490346003401538E-4 0.0 0.20013697746117065 0.0 60-61 0.0 1.2490346003401538E-4 0.0 0.24593491280697627 0.0 62-63 0.0 1.2490346003401538E-4 0.0 0.2853627716910472 0.0 64-65 0.0 1.2490346003401538E-4 0.0 0.3314729656869378 0.0 66-67 0.0 2.0817243339002562E-4 0.0 0.40545744851375287 0.0 68-69 0.0 2.0817243339002562E-4 0.0 0.48377191795508057 0.0 70-71 0.0 2.289896767290282E-4 0.0 0.5584433698120828 0.0 72-73 0.0 2.4980692006803076E-4 0.0 0.641462536248025 0.0 74-75 0.0 2.4980692006803076E-4 0.0 0.7209427713163368 0.0 76-77 0.0 2.9144140674603586E-4 0.0 0.7957391266333729 0.0 78-79 0.0 2.9144140674603586E-4 0.0 0.885836155804576 0.0 80-81 0.0 2.9144140674603586E-4 0.0 1.024437361955655 0.0 82-83 0.0 2.9144140674603586E-4 0.0 1.169949892895283 0.0 84-85 0.0 2.9144140674603586E-4 0.0 1.3131308925809426 0.0 86-87 0.0 2.9144140674603586E-4 0.0 1.5226564467880035 0.0 88-89 0.0 3.1225865008503847E-4 0.0 1.7854949611862498 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17680 0.0 28.00781 1 GGTATCA 12615 0.0 27.499786 1 GTACATG 11860 0.0 24.281841 1 TACATGG 12230 0.0 23.149021 2 TATCAAC 21310 0.0 22.95972 2 ATCAACG 21285 0.0 22.919739 3 TCAACGC 21315 0.0 22.86519 4 TAGGACG 125 2.2562208E-4 22.801008 4 GAGTACT 11710 0.0 22.677958 12-13 CAACGCA 21595 0.0 22.634714 5 AACGCAG 22125 0.0 22.175613 6 ATACCGT 175 4.0551204E-6 21.712534 6 ACATGGG 12390 0.0 21.661573 3 GTACTTT 12300 0.0 21.470259 14-15 AGTACTT 12015 0.0 21.430117 12-13 AGAGTAC 18700 0.0 20.615597 10-11 GGACCGA 485 0.0 20.565351 6 AGGACCG 595 0.0 19.958866 5 ACGCAGA 24670 0.0 19.892078 7 CGCAGAG 24720 0.0 19.794191 8 >>END_MODULE