FastQCFastQC Report
Thu 26 May 2016
SRR937636_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937636_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205147
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111210.504320120155255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84650.3838746351150286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55060.24968856951486681No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38170.17309503629463252No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31460.14266622587972594No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22200.10067356053814099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA145300.028.7657831
GGTATCA102600.028.3169731
GTACATG113200.025.4973581
TACATGG114750.024.5616422
TATCAAC172900.024.0529562
ATCAACG173700.023.7232763
TCAACGC174550.023.6643644
CAACGCA175950.023.4220395
AACGCAG179950.022.993996
GAGTACT92400.022.7462812-13
ACATGGG118050.022.7074573
GTACCTG17500.020.9221171
GTACTTT99450.020.91935314-15
AGTACTT97150.020.60743112-13
TACCTGG17600.020.5237312
ACGCAGA203200.020.1526247
CGCAGAG203300.020.1427128
CATGGGG48600.020.0499134
AGAGTAC160750.019.35393510-11
GTATAGG6800.018.8802551
Produced by FastQC (version 0.11.5)