FastQCFastQC Report
Thu 26 May 2016
SRR937636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937636_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205147
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131350.5956518998506676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106610.48345983283654104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76920.3488202827294507No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40640.184296103615768No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28320.12842681236216905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA161700.029.7103231
GGTATCA112300.028.5197941
GTACATG108900.025.832121
TACATGG112100.024.6613142
ATCAACG196050.024.4226343
TATCAAC196700.024.3177782
TCAACGC197250.024.2740544
CAACGCA199550.024.0180765
GGACCGA4600.023.7492076
AACGCAG202950.023.6380376
ACATGGG112400.023.2854213
GAGTACT114650.022.80824712-13
AGTACTT118500.021.7264912-13
GTACTTT120250.021.54709814-15
ACGCAGA228550.020.846277
CGCAGAG228350.020.8229268
AGGACCG5050.020.6933175
AGAGTAC180550.020.52134310-11
CATGGGA67750.020.2622344
ATGGGAG29700.019.9918215
Produced by FastQC (version 0.11.5)