##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937636_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2205147 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19881849146565 33.0 31.0 34.0 31.0 34.0 2 32.423574936274086 34.0 31.0 34.0 31.0 34.0 3 32.53106482243587 34.0 31.0 34.0 31.0 34.0 4 36.15582362536375 37.0 35.0 37.0 35.0 37.0 5 35.997120373381 37.0 35.0 37.0 35.0 37.0 6 36.09656499090537 37.0 35.0 37.0 35.0 37.0 7 36.05852852440223 37.0 35.0 37.0 35.0 37.0 8 36.05469975471023 37.0 35.0 37.0 35.0 37.0 9 37.71718756164555 39.0 37.0 39.0 35.0 39.0 10-11 37.66906877409987 39.0 37.0 39.0 35.0 39.0 12-13 37.58783087930192 39.0 37.0 39.0 35.0 39.0 14-15 38.80276598340156 40.0 38.0 41.0 35.0 41.0 16-17 38.781657866799804 40.0 38.0 41.0 35.0 41.0 18-19 38.78192973076171 40.0 38.0 41.0 35.0 41.0 20-21 38.710517711517646 40.0 38.0 41.0 34.5 41.0 22-23 38.560711598818585 40.0 38.0 41.0 34.0 41.0 24-25 38.50447453162987 40.0 38.0 41.0 34.0 41.0 26-27 38.431890935162144 40.0 38.0 41.0 34.0 41.0 28-29 38.32362445678224 40.0 38.0 41.0 34.0 41.0 30-31 38.3810809438101 40.0 38.0 41.0 34.0 41.0 32-33 38.29450122826279 40.0 38.0 41.0 34.0 41.0 34-35 38.153030160801066 40.0 38.0 41.0 34.0 41.0 36-37 37.9930752915792 40.0 37.0 41.0 33.5 41.0 38-39 37.86058571151946 40.0 37.0 41.0 33.0 41.0 40-41 37.675283779267325 40.0 37.0 41.0 33.0 41.0 42-43 37.455977084520896 40.0 37.0 41.0 33.0 41.0 44-45 37.13038722588562 40.0 37.0 41.0 32.0 41.0 46-47 36.7357659149254 40.0 36.0 41.0 31.0 41.0 48-49 36.371009279653464 39.0 36.0 41.0 30.0 41.0 50-51 36.109914214335824 39.0 36.0 41.0 30.0 41.0 52-53 35.889901217469856 39.0 35.0 40.5 29.0 41.0 54-55 35.687668441151544 39.0 35.0 40.0 28.5 41.0 56-57 35.41274277859934 38.5 35.0 40.0 28.0 41.0 58-59 35.15093732980159 38.0 35.0 40.0 27.5 41.0 60-61 34.80497218552777 38.0 34.0 40.0 26.5 41.0 62-63 34.44662759444155 37.5 34.0 40.0 26.0 41.0 64-65 34.03812852385805 37.0 34.0 39.5 26.0 41.0 66-67 33.61195308067897 36.5 33.0 39.0 25.0 40.5 68-69 33.158880791167206 36.0 32.5 39.0 23.5 40.0 70-71 32.73529837239876 35.0 32.0 38.5 22.5 40.0 72-73 32.28416404892735 35.0 32.0 37.5 22.0 39.5 74-75 31.786373878929616 35.0 31.0 37.0 20.5 39.0 76-77 30.55174711708562 34.0 29.5 35.5 20.0 38.0 78-79 31.123423517797228 34.0 31.0 36.0 20.0 38.5 80-81 30.909761798193045 34.0 31.0 36.0 18.5 37.5 82-83 30.550037933979006 34.0 31.0 35.0 17.5 37.0 84-85 30.142689580331833 34.0 30.0 35.0 13.0 36.5 86-87 29.711999698886288 34.0 30.0 35.0 7.0 36.0 88-89 29.27181453209242 34.0 29.0 35.0 4.5 36.0 90-91 28.74855372453628 34.0 29.0 35.0 2.0 35.0 92-93 28.289234685941572 33.5 29.0 35.0 2.0 35.0 94-95 27.80807719394671 33.0 27.0 35.0 2.0 35.0 96-97 27.247399152981636 33.0 26.5 35.0 2.0 35.0 98-99 26.406189927474223 32.5 25.0 34.0 2.0 35.0 100-101 23.94378515355212 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2874879025650401 1101 2 0.2794614503154307 1101 3 0.29270467543958034 1101 4 0.15166791866391804 1101 5 0.10174875801845218 1101 6 0.07172224761751522 1101 7 0.05094295586454933 1101 8 0.08680123982408361 1101 9 0.2439231623783158 1101 10-11 0.15458697972653823 1101 12-13 0.20210401466561478 1101 14-15 0.2750744539325183 1101 16-17 0.22822743337464146 1101 18-19 0.31650539909641395 1101 20-21 0.352785074764725 1101 22-23 0.32280669624949354 1101 24-25 0.24658839850368253 1101 26-27 0.16626546728028302 1101 28-29 0.20019837407949126 1101 30-31 0.2170273493010768 1101 32-33 0.20737141037295004 1101 34-35 0.23184468849775897 1101 36-37 0.28836494375743627 1101 38-39 0.4452335581152411 1101 40-41 0.29434696449552433 1101 42-43 0.16265971986295824 1101 44-45 0.33745852872981885 1101 46-47 0.2575834601784308 1101 48-49 0.3504638340429622 1101 50-51 0.5343230129623819 1101 52-53 0.561560338213333 1101 54-55 0.45224295245741786 1101 56-57 0.48628559511873704 1101 58-59 0.4183954236801597 1101 60-61 0.6522390753543519 1101 62-63 0.5568018133426733 1101 64-65 0.39877388226936716 1101 66-67 0.4854975409277884 1101 68-69 0.5200307654157967 1101 70-71 0.49669973841591997 1101 72-73 0.5035725498847228 1101 74-75 0.4853193590148912 1101 76-77 0.4152699069643617 1101 78-79 0.5481742225630448 1101 80-81 0.602676169957622 1101 82-83 0.605396668971796 1101 84-85 0.7394913820512521 1101 86-87 0.8173125574823352 1101 88-89 0.9317081314992279 1101 90-91 0.8548987461152073 1101 92-93 1.1417765484696716 1101 94-95 1.2378529097007487 1101 96-97 1.3767267508285777 1101 98-99 1.6988298463007538 1101 100-101 2.3472472233941204 1102 1 0.32920038571222676 1102 2 0.37040044274257866 1102 3 0.30286779444304557 1102 4 0.15192764816045923 1102 5 0.10052817737873454 1102 6 0.10585447731014597 1102 7 0.1306395829329503 1102 8 0.15404565046011243 1102 9 0.3045489106730557 1102 10-11 0.23123252518067972 1102 12-13 0.2146115438611318 1102 14-15 0.2929850596276182 1102 16-17 0.2936328333802649 1102 18-19 0.2914130138324751 1102 20-21 0.2581682460692747 1102 22-23 0.3663809441069148 1102 24-25 0.32791795453643147 1102 26-27 0.3169522335074504 1102 28-29 0.2649788693562698 1102 30-31 0.35200603076319936 1102 32-33 0.42164639509256574 1102 34-35 0.2734707801386165 1102 36-37 0.42950480106160427 1102 38-39 0.3906089671336943 1102 40-41 0.32528733326702053 1102 42-43 0.47684694413161566 1102 44-45 0.5344626118846989 1102 46-47 0.531418821276425 1102 48-49 0.5179830695650693 1102 50-51 0.5690266845459107 1102 52-53 0.4420531360845814 1102 54-55 0.3718326242510912 1102 56-57 0.48291558640189436 1102 58-59 0.5190833368609447 1102 60-61 0.5163924089935392 1102 62-63 0.5855847993587204 1102 64-65 0.7172845666972876 1102 66-67 0.7903870411964036 1102 68-69 0.7090218976144129 1102 70-71 0.7819145443291688 1102 72-73 0.6939653703990629 1102 74-75 0.6311504416059073 1102 76-77 0.5763275387937341 1102 78-79 0.6433604582316761 1102 80-81 0.6424305072922181 1102 82-83 0.5448103240835813 1102 84-85 0.7152519972919684 1102 86-87 0.8809692232067725 1102 88-89 0.8778146309523933 1102 90-91 0.8874171010729306 1102 92-93 1.1416770224945054 1102 94-95 1.0620713533068873 1102 96-97 1.1459360040063764 1102 98-99 1.5805596891459075 1102 100-101 2.169549514489468 1103 1 0.19620717485245365 1103 2 0.20652954906014287 1103 3 0.19194249780139927 1103 4 0.1120826247956046 1103 5 -0.010591053955408825 1103 6 0.09896943922907298 1103 7 0.08472162301183062 1103 8 0.08094189682562103 1103 9 0.1145335527565976 1103 10-11 0.030325630944950888 1103 12-13 0.17229046643983992 1103 14-15 0.23262752105814144 1103 16-17 0.19649811151256102 1103 18-19 0.2228214502767898 1103 20-21 0.2316742359418953 1103 22-23 0.21459282346241793 1103 24-25 0.3054388203407541 1103 26-27 0.24864907650733414 1103 28-29 0.17878398923214434 1103 30-31 0.23116154499672348 1103 32-33 0.25815731033597444 1103 34-35 0.2597992755618961 1103 36-37 0.17593857735509033 1103 38-39 0.1859925371576665 1103 40-41 0.2121852623011833 1103 42-43 0.24674057950871742 1103 44-45 0.3681753774932375 1103 46-47 0.40099328435540116 1103 48-49 0.31179484168610827 1103 50-51 0.2608521590085573 1103 52-53 0.15365994918145276 1103 54-55 0.23805012771268963 1103 56-57 0.3554254506903902 1103 58-59 0.27425986021082593 1103 60-61 0.27139264428195276 1103 62-63 0.29117090279721225 1103 64-65 0.26429643949324344 1103 66-67 0.20327685349328561 1103 68-69 0.0964693676935724 1103 70-71 0.2773852423657175 1103 72-73 0.2619369846344384 1103 74-75 0.3634870551924614 1103 76-77 0.2257052009395366 1103 78-79 0.3079655587140877 1103 80-81 0.4534965848347241 1103 82-83 0.6227194870700039 1103 84-85 0.6285230837683038 1103 86-87 0.6492197611616497 1103 88-89 0.8397171588055485 1103 90-91 0.8278909148984788 1103 92-93 0.9705574631825726 1103 94-95 1.0148698237895992 1103 96-97 1.2838264223491755 1103 98-99 1.3721244569162465 1103 100-101 2.081363595288934 1104 1 0.19616834412596518 1104 2 0.17158457320381615 1104 3 0.1247765550265072 1104 4 0.054710728533898134 1104 5 -0.08458300327461643 1104 6 9.804356891223165E-4 1104 7 0.05901785793772518 1104 8 0.07324052519715707 1104 9 0.11942990651790808 1104 10-11 0.10589750544806265 1104 12-13 0.12886013997719914 1104 14-15 0.1325465958156684 1104 16-17 0.0798798995670893 1104 18-19 0.13416010609763163 1104 20-21 0.15248951382427833 1104 22-23 0.14902563542630531 1104 24-25 0.040331157130360396 1104 26-27 0.05490977797101948 1104 28-29 0.04698003418875629 1104 30-31 0.08038534890155802 1104 32-33 0.10145532779306876 1104 34-35 0.021114080613237718 1104 36-37 0.11364873610915538 1104 38-39 -0.011799802142540727 1104 40-41 -0.025028214638894042 1104 42-43 0.10619602878190193 1104 44-45 0.03346137750997258 1104 46-47 0.0046312380430677536 1104 48-49 0.14462730885991704 1104 50-51 0.1691616834158438 1104 52-53 0.09913123201810237 1104 54-55 0.05025644942190155 1104 56-57 0.238902390762199 1104 58-59 0.147525156085905 1104 60-61 0.20758053813347743 1104 62-63 0.23157812330385497 1104 64-65 0.18824746591320007 1104 66-67 0.2197568146625528 1104 68-69 0.10465838294969387 1104 70-71 0.059216479341948514 1104 72-73 0.03176157686996106 1104 74-75 0.02465189330013473 1104 76-77 -0.14086999593392235 1104 78-79 -0.1685346546875337 1104 80-81 0.06540891442725183 1104 82-83 0.21440619675174233 1104 84-85 0.1618180884966165 1104 86-87 0.25331749073576404 1104 88-89 0.35040021136929766 1104 90-91 0.2514995781674578 1104 92-93 0.5687388629298376 1104 94-95 0.710465883809853 1104 96-97 0.9211301137788013 1104 98-99 1.0263282178060038 1104 100-101 1.731075879690529 1105 1 0.16823183618945592 1105 2 0.12112979500710708 1105 3 0.0734410804652228 1105 4 0.09033145189656011 1105 5 0.030955794844828688 1105 6 0.05963146208208059 1105 7 0.05406138072318356 1105 8 0.0518543128432043 1105 9 0.15520330874291943 1105 10-11 0.17949709582185136 1105 12-13 0.1328502717092661 1105 14-15 0.17042864208480069 1105 16-17 0.19698924183771993 1105 18-19 0.1714900879437451 1105 20-21 0.14022958737080415 1105 22-23 0.2734226004684288 1105 24-25 0.14036653393993248 1105 26-27 0.1493072084974827 1105 28-29 0.17253977910333873 1105 30-31 0.21375592098180363 1105 32-33 0.24979308322759408 1105 34-35 0.20208321900172876 1105 36-37 0.4511109155068169 1105 38-39 0.36716496069318083 1105 40-41 0.2783791307039323 1105 42-43 0.325953045958272 1105 44-45 0.529522449054916 1105 46-47 0.5333488286961412 1105 48-49 0.4844820777469394 1105 50-51 0.5747018779882964 1105 52-53 0.5963988389631325 1105 54-55 0.6347502352060204 1105 56-57 0.7008322888211254 1105 58-59 0.7093628882462113 1105 60-61 0.601687506433997 1105 62-63 0.5799708058900777 1105 64-65 0.6174901228977916 1105 66-67 0.6864076548617888 1105 68-69 0.6100728967513049 1105 70-71 0.5847408474469589 1105 72-73 0.7398842785410551 1105 74-75 0.9136608771478301 1105 76-77 0.6461718511079262 1105 78-79 0.784667412062916 1105 80-81 0.9147344276237348 1105 82-83 0.866804837537483 1105 84-85 0.9008387094527208 1105 86-87 0.9481831985046973 1105 88-89 1.0908321808335266 1105 90-91 0.9756712487907393 1105 92-93 1.174266954909541 1105 94-95 1.4577935382342808 1105 96-97 1.5424828112605304 1105 98-99 1.8501600848636741 1105 100-101 2.4108496543030142 1106 1 -0.02795679541861773 1106 2 0.06995899653163917 1106 3 -0.03703490175166024 1106 4 0.012312310263311588 1106 5 -0.10288372016388081 1106 6 -0.11922292952090174 1106 7 -0.08006188758675847 1106 8 -0.03344981361036048 1106 9 0.06910723909803096 1106 10-11 0.08369707235549129 1106 12-13 -0.11670926916078628 1106 14-15 0.014770432047484405 1106 16-17 0.06492448658237748 1106 18-19 0.18590767499566851 1106 20-21 0.09558706725472632 1106 22-23 -0.024073397512943018 1106 24-25 -0.01057497476112701 1106 26-27 -0.13859221936586152 1106 28-29 0.04920871244939207 1106 30-31 -0.06062394510374247 1106 32-33 -0.04003655508363835 1106 34-35 -0.1274651874334083 1106 36-37 0.010770418983163665 1106 38-39 -0.022514135892826914 1106 40-41 -0.020323376645109192 1106 42-43 0.014299971000355072 1106 44-45 0.15972588678380362 1106 46-47 0.30978423270338595 1106 48-49 0.2932009278729524 1106 50-51 0.234511049244567 1106 52-53 0.24173436050671882 1106 54-55 0.17821432377126456 1106 56-57 0.18574659848523112 1106 58-59 0.21398038749453008 1106 60-61 0.3521594833781947 1106 62-63 0.2261518230173678 1106 64-65 0.18871499069496878 1106 66-67 0.2805737725234607 1106 68-69 -0.09432263898738569 1106 70-71 0.28670467689672563 1106 72-73 0.47506007449602095 1106 74-75 0.3346391157241051 1106 76-77 0.41155249797991544 1106 78-79 0.4513112073953458 1106 80-81 0.577355006506501 1106 82-83 0.5773019989817705 1106 84-85 0.6395668141015314 1106 86-87 0.6716136345978221 1106 88-89 0.7462575867206809 1106 90-91 0.8689475599829457 1106 92-93 1.1142832549189698 1106 94-95 1.130619752725373 1106 96-97 1.4280778871180537 1106 98-99 1.4560937829111182 1106 100-101 2.266627669223677 1107 1 0.07404800278148826 1107 2 0.05317983617839417 1107 3 0.03747073823792846 1107 4 0.042965691961278196 1107 5 -0.19114343517987464 1107 6 -0.007170034530318503 1107 7 0.05122092307183124 1107 8 -0.008531505454179467 1107 9 0.21404502320199015 1107 10-11 0.11401488615165078 1107 12-13 0.08861371016180186 1107 14-15 0.08191615346107994 1107 16-17 0.09159228201223613 1107 18-19 0.10747776544977938 1107 20-21 0.12771243266789156 1107 22-23 0.07444447903480267 1107 24-25 0.08108394361590143 1107 26-27 0.062424145787112195 1107 28-29 -0.04434920649393348 1107 30-31 0.02029086962603799 1107 32-33 0.04527020065966525 1107 34-35 0.17805896044605163 1107 36-37 0.14656627355255836 1107 38-39 4.2761424161597006E-5 1107 40-41 0.02827638306225566 1107 42-43 0.0626654645185809 1107 44-45 -0.07374299378922444 1107 46-47 0.09106627809879342 1107 48-49 0.0064742263102814945 1107 50-51 0.05240663291079528 1107 52-53 -0.026889753701155428 1107 54-55 -0.23336946489366284 1107 56-57 -0.15250565521481718 1107 58-59 -0.10746831308860294 1107 60-61 -0.08919323407133106 1107 62-63 -0.06553655799186942 1107 64-65 -0.06516728504465874 1107 66-67 0.11445857363155909 1107 68-69 2.661851092256029E-4 1107 70-71 0.10511250860004395 1107 72-73 0.17939959498212232 1107 74-75 0.17938587100824677 1107 76-77 0.06737930744468912 1107 78-79 0.15750113423618473 1107 80-81 0.06675687680969844 1107 82-83 0.0698759807991145 1107 84-85 0.12203578267982707 1107 86-87 0.29180146844388233 1107 88-89 0.4884988703731281 1107 90-91 0.5411995955829916 1107 92-93 0.7645378005822252 1107 94-95 0.6494697152274753 1107 96-97 1.0626866271887643 1107 98-99 1.249873149885417 1107 100-101 1.9688384188754817 1108 1 -0.07940027308058006 1108 2 0.05878328445425751 1108 3 -0.03709822727930856 1108 4 -0.010482583900788711 1108 5 -0.20795378000746467 1108 6 -0.13389417246135338 1108 7 -0.07421011141092748 1108 8 -0.11370391924728551 1108 9 -0.0036273905911130555 1108 10-11 0.012937299944759673 1108 12-13 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2316 82-83 -0.7022680002309372 2316 84-85 -0.6678653785647448 2316 86-87 -0.700062063157926 2316 88-89 -0.6511136418155061 2316 90-91 -0.7259027224739221 2316 92-93 -0.8176385357354761 2316 94-95 -0.9033358871127426 2316 96-97 -0.9923688865068314 2316 98-99 -1.156594583471552 2316 100-101 -1.63395333274228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 13.0 5 49.0 6 70.0 7 192.0 8 452.0 9 923.0 10 1665.0 11 2720.0 12 4382.0 13 6419.0 14 8675.0 15 12117.0 16 17202.0 17 25044.0 18 22639.0 19 12523.0 20 10166.0 21 10288.0 22 10796.0 23 11609.0 24 13402.0 25 16108.0 26 19952.0 27 24397.0 28 30889.0 29 38327.0 30 49014.0 31 61917.0 32 80149.0 33 106769.0 34 147812.0 35 214545.0 36 333500.0 37 505737.0 38 376530.0 39 28142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33575449839917 19.560125271473698 15.272485417433204 21.831634812693924 2 15.419704636337004 20.704161991995086 40.45464563344105 23.42148773822686 3 19.832963516717932 24.774584188718485 30.037498633877924 25.35495366068566 4 12.858604373587962 17.034835788650728 37.04335223155292 33.06320760620839 5 13.433411886788688 37.89646364317501 33.8915073374007 14.778617132635604 6 31.756114218235794 36.48641111000763 17.113008792611105 14.644465879145471 7 28.617865384938057 33.368478382620296 20.068412672715244 17.945243559726404 8 26.633009046562428 35.38190424493242 19.09323958901606 18.891847119489086 9 26.54394468940166 17.167290888090452 20.277514378860005 36.011250043647884 10-11 25.03087095780916 27.34835818201689 26.513629250113485 21.107141610060463 12-13 26.111443679537068 24.51423090528149 27.844729848620897 21.52959556656055 14-15 22.735604474440933 25.638177409487895 26.7569917107567 24.869226405314475 16-17 21.49802258080754 29.24750594858302 27.28214944400532 21.97232202660412 18-19 21.43668880124545 27.35504707849409 30.370084171259332 20.83817994900113 20-21 22.122936670561348 26.116236879822107 30.75375820213061 21.007068247485936 22-23 21.859894673401836 26.176942880296544 30.653232605080582 21.309929841221038 24-25 21.835307135390803 26.336074662095516 30.85502701648652 20.97359118602716 26-27 21.59669294134533 26.50753264645641 30.819919173788602 21.07585523840966 28-29 21.194371664680734 26.8116870380721 30.939110264081894 21.054831033165275 30-31 21.413580465258864 26.433311656685433 30.89872379869196 21.254384079363742 32-33 21.31442937817751 26.57008807122609 30.77710919045306 21.338373360143336 34-35 21.82537037213392 26.444790301961728 30.68877040850338 21.04106891740097 36-37 21.700548761601834 26.29534448270342 30.718292249904426 21.285814505790317 38-39 21.488771496866196 26.672235456411748 30.771939467074077 21.06705357964798 40-41 21.713495744274645 26.690442859364932 30.55082042149571 21.045240974864715 42-43 21.54711279491629 26.658938517905245 30.29692826495577 21.49702042222269 44-45 21.61944305753766 26.699467201052812 30.09738126301784 21.58370847839169 46-47 21.850062603536184 27.06529315279208 29.62884107045925 21.45580317321249 48-49 21.955339031819648 27.22965861232834 29.294464269275473 21.52053808657654 50-51 21.87799724916298 27.307136440337082 29.16816883409587 21.646697476404068 52-53 22.077233016422955 27.673100861848088 28.720310909259933 21.52935521246902 54-55 21.841423723679192 27.373821337080926 28.91049893725906 21.87425600198082 56-57 22.010754838566317 27.67389203531556 28.788012771937655 21.527340354180467 58-59 22.194937571055355 27.387856682570366 28.755565955466917 21.661639790907365 60-61 21.997830530118854 27.34672563779195 29.319655333635353 21.335788498453844 62-63 21.944410055202667 27.38620146412008 29.25575936660912 21.413629114068133 64-65 21.816912886079702 27.47703441085787 29.078106811019854 21.627945892042572 66-67 21.78265213158125 27.58652824505577 28.78048492912264 21.85033469424034 68-69 21.701886540897274 27.537960054363726 28.974961759918955 21.785191644820053 70-71 21.850289345789644 27.581494567028862 28.78830753686716 21.779908550314335 72-73 21.9385070055468 27.41970994657722 28.829503540696066 21.812279507179912 74-75 21.703647219650584 27.958585952718312 28.40164061736516 21.936126210265943 76-77 21.793785176226347 27.635232481099898 28.595327204943704 21.975655137730048 78-79 21.869721156911535 27.632080763776745 28.55462697044687 21.94357110886485 80-81 21.782538760454518 27.894557596387 28.490345541589747 21.83255810156874 82-83 21.835096824808375 27.787251844268567 28.60855931079284 21.769092020130216 84-85 21.773019605993625 27.73206895519654 28.431729113136477 22.06318232567336 86-87 21.774761501160693 27.643395202224614 28.507782020881145 22.074061275733545 88-89 21.80528100847699 27.611764657866345 28.429329201182508 22.15362513247416 90-91 21.80121525022018 27.56131807210775 28.510352484242475 22.127114193429595 92-93 21.967823030978604 27.517741367515512 28.504777476983328 22.009658124522552 94-95 21.673997243721168 27.46451823846664 28.567778021147795 22.293706496664395 96-97 21.50759110390373 27.50991203761019 28.672555616473637 22.30994124201244 98-99 21.641657706552365 27.537828612475472 28.268733592333923 22.551780088638242 100-101 21.34419198155363 27.42894903279251 28.740563255526325 22.486295730127534 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 509.0 1 371.0 2 314.0 3 439.5 4 414.5 5 405.5 6 752.0 7 1807.0 8 3122.0 9 3788.5 10 3675.5 11 3365.5 12 3482.5 13 3823.0 14 4162.5 15 4413.0 16 4609.5 17 4815.5 18 5029.0 19 5309.5 20 5684.0 21 6283.0 22 7225.5 23 8546.5 24 10210.5 25 12285.0 26 14713.5 27 17342.5 28 19684.0 29 22677.5 30 26331.5 31 29530.5 32 33290.0 33 38148.5 34 44092.0 35 48826.0 36 53763.0 37 59033.0 38 62244.5 39 64252.0 40 67169.0 41 71290.0 42 75501.5 43 79998.5 44 83635.0 45 85821.0 46 85726.0 47 89399.5 48 97426.0 49 95200.5 50 86548.5 51 80274.0 52 73904.5 53 69734.5 54 66316.5 55 60936.5 56 53866.5 57 46499.0 58 40644.5 59 35562.5 60 29847.5 61 23635.5 62 17984.0 63 13355.5 64 9916.5 65 7556.5 66 5486.5 67 4085.5 68 2810.5 69 1729.5 70 1211.0 71 885.0 72 701.5 73 594.0 74 473.0 75 356.0 76 227.5 77 117.0 78 77.0 79 49.5 80 27.0 81 19.5 82 9.5 83 3.0 84 3.0 85 4.0 86 2.5 87 1.5 88 2.0 89 2.0 90 1.0 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04416939097484204 2 4.5348450692856306E-5 3 0.0 4 4.5348450692856306E-5 5 0.012652217743306908 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003945315210278499 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.5871957742499707E-4 22-23 2.720907041571378E-4 24-25 5.441814083142756E-4 26-27 0.002063354506524962 28-29 0.0024714905627606686 30-31 4.081360562357067E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009999333377774814 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 9.069690138571261E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.802267603928446E-5 74-75 6.802267603928446E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.0406802811785335E-4 84-85 2.2674225346428154E-4 86-87 0.0 88-89 0.0 90-91 0.007663888167092715 92-93 0.002743581266917806 94-95 0.0 96-97 0.0 98-99 1.8139380277142522E-4 100-101 0.012176059011031918 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2205147.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.93507789946441 #Duplication Level Percentage of deduplicated Percentage of total 1 74.76557302563134 38.82955859284259 2 13.923901720684597 14.462778410564825 3 4.657431139004534 7.256521470467753 4 2.1203067933181363 4.40473193926964 5 1.1605361667428948 3.0136268112469033 6 0.6931482603621605 2.1599225338672228 7 0.4689664293962588 1.7049065630049869 8 0.3365788692795873 1.3984199836279214 9 0.2551914213477316 1.1928047712272538 >10 1.4547867388942826 13.827937217181935 >50 0.1076137938383781 3.867934290333175 >100 0.052470221476499775 4.775067387873844 >500 0.0022720230152929485 0.8203562637726899 >1k 9.61240506470065E-4 0.856390943648502 >5k 8.738550058818774E-5 0.3490916342630777 >10k+ 1.7477100117637548E-4 1.079951186807618 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13135 0.5956518998506676 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10661 0.48345983283654104 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7692 0.3488202827294507 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4064 0.184296103615768 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2832 0.12842681236216905 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 4.5348450692856306E-5 0.0 0.0 0.0 8 0.0 4.5348450692856306E-5 0.0 0.0 0.0 9 0.0 4.5348450692856306E-5 0.0 0.0 0.0 10-11 0.0 4.5348450692856306E-5 0.0 0.0 0.0 12-13 0.0 4.5348450692856306E-5 0.0 4.5348450692856306E-5 0.0 14-15 0.0 9.069690138571261E-5 0.0 4.5348450692856306E-5 0.0 16-17 0.0 9.069690138571261E-5 0.0 4.5348450692856306E-5 0.0 18-19 0.0 9.069690138571261E-5 0.0 6.802267603928446E-5 0.0 20-21 0.0 9.069690138571261E-5 0.0 9.069690138571261E-5 0.0 22-23 0.0 9.069690138571261E-5 0.0 2.4941647881070964E-4 0.0 24-25 0.0 9.069690138571261E-5 0.0 4.308102815821349E-4 2.2674225346428153E-5 26-27 0.0 9.069690138571261E-5 0.0 7.029009857392728E-4 4.5348450692856306E-5 28-29 0.0 9.069690138571261E-5 0.0 0.0012924308447464046 4.5348450692856306E-5 30-31 0.0 9.069690138571261E-5 0.0 0.002675558590878522 4.5348450692856306E-5 32-33 0.0 9.069690138571261E-5 0.0 0.004376125491860634 4.5348450692856306E-5 34-35 0.0 1.1337112673214076E-4 0.0 0.006280760420960598 4.5348450692856306E-5 36-37 0.0 1.360453520785689E-4 0.0 0.008593531406296269 4.5348450692856306E-5 38-39 0.0 1.360453520785689E-4 0.0 0.010588863236781949 4.5348450692856306E-5 40-41 0.0 1.360453520785689E-4 0.0 0.013083028024889042 4.5348450692856306E-5 42-43 0.0 1.360453520785689E-4 0.0 0.017595198868828245 4.5348450692856306E-5 44-45 0.0 1.360453520785689E-4 0.0 0.02469223140226026 4.5348450692856306E-5 46-47 0.0 1.360453520785689E-4 0.0 0.038818273793084994 4.5348450692856306E-5 48-49 0.0 1.360453520785689E-4 0.0 0.06582327618068093 4.5348450692856306E-5 50-51 0.0 1.360453520785689E-4 0.0 0.08974458392116264 4.5348450692856306E-5 52-53 0.0 1.360453520785689E-4 0.0 0.11112637842284437 4.5348450692856306E-5 54-55 0.0 1.360453520785689E-4 0.0 0.13677092728965462 4.5348450692856306E-5 56-57 0.0 1.360453520785689E-4 0.0 0.15921841038261847 4.5348450692856306E-5 58-59 0.0 1.360453520785689E-4 0.0 0.18894431981178578 4.5348450692856306E-5 60-61 0.0 1.360453520785689E-4 0.0 0.2351770652931528 4.5348450692856306E-5 62-63 0.0 1.360453520785689E-4 0.0 0.27576392866325916 4.5348450692856306E-5 64-65 0.0 1.360453520785689E-4 0.0 0.32088563710265117 4.5348450692856306E-5 66-67 0.0 1.360453520785689E-4 0.0 0.3967989435624927 4.5348450692856306E-5 68-69 0.0 1.360453520785689E-4 0.0 0.4816912432595196 4.5348450692856306E-5 70-71 0.0 1.5871957742499707E-4 0.0 0.5586702383106432 4.5348450692856306E-5 72-73 0.0 1.8139380277142522E-4 0.0 0.6442654389934095 4.5348450692856306E-5 74-75 0.0 1.8139380277142522E-4 0.0 0.7277292624936116 4.5348450692856306E-5 76-77 0.0 1.8139380277142522E-4 0.0 0.8038466369815709 4.5348450692856306E-5 78-79 0.0 1.8139380277142522E-4 0.0 0.8969923547046977 4.5348450692856306E-5 80-81 0.0 1.8139380277142522E-4 0.0 1.0391824218521486 4.5348450692856306E-5 82-83 0.0 1.8139380277142522E-4 0.0 1.1863154701251208 4.5348450692856306E-5 84-85 0.0 1.8139380277142522E-4 0.0 1.3328816627644327 4.5348450692856306E-5 86-87 0.0 1.8139380277142522E-4 0.0 1.5500100446818283 4.5348450692856306E-5 88-89 0.0 1.8139380277142522E-4 0.0 1.8308983482733803 4.5348450692856306E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16170 0.0 29.710323 1 GGTATCA 11230 0.0 28.519794 1 GTACATG 10890 0.0 25.83212 1 TACATGG 11210 0.0 24.661314 2 ATCAACG 19605 0.0 24.422634 3 TATCAAC 19670 0.0 24.317778 2 TCAACGC 19725 0.0 24.274054 4 CAACGCA 19955 0.0 24.018076 5 GGACCGA 460 0.0 23.749207 6 AACGCAG 20295 0.0 23.638037 6 ACATGGG 11240 0.0 23.285421 3 GAGTACT 11465 0.0 22.808247 12-13 AGTACTT 11850 0.0 21.72649 12-13 GTACTTT 12025 0.0 21.547098 14-15 ACGCAGA 22855 0.0 20.84627 7 CGCAGAG 22835 0.0 20.822926 8 AGGACCG 505 0.0 20.693317 5 AGAGTAC 18055 0.0 20.521343 10-11 CATGGGA 6775 0.0 20.262234 4 ATGGGAG 2970 0.0 19.991821 5 >>END_MODULE