FastQCFastQC Report
Thu 26 May 2016
SRR937635_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937635_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122755
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52250.4653731223641845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43260.38530222532965785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28710.25571028407800456No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14740.13128420715115943No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.10073435433375937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29700.051.5640371
GCGTACC459.5794816E-442.2628822
GTATCAA50300.039.8071981
ATCAACG63900.030.8773063
TCAACGC64050.030.732144
TATCAAC64700.030.496882
CAACGCA65750.029.8665685
AACGCAG67350.029.1310046
GCGCGTT350.00839224227.1435212-13
TACATGG50300.025.6160952
GTACATG52350.025.347491
CGCAGAG76850.025.283778
ACGCAGA77800.025.1582247
GTACCTG9950.024.85581
TACCTGG9600.024.7634072
ACATGGG51600.023.587533
GCAGAGT81750.023.0128179
GTACCGG1252.2364555E-422.8290961
GAGTACT47650.022.52940612-13
TACACGG2755.0931703E-1122.4771735
Produced by FastQC (version 0.11.5)