##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937635_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1122755 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.546555570894807 33.0 31.0 34.0 30.0 34.0 2 31.681472805732326 33.0 31.0 34.0 30.0 34.0 3 31.690247649754397 33.0 31.0 34.0 30.0 34.0 4 35.29424139727723 37.0 35.0 37.0 33.0 37.0 5 35.27790657801568 37.0 35.0 37.0 33.0 37.0 6 35.33920668355964 37.0 35.0 37.0 33.0 37.0 7 35.27662312793085 37.0 35.0 37.0 33.0 37.0 8 35.26032838865113 37.0 35.0 37.0 33.0 37.0 9 36.86390886702798 39.0 37.0 39.0 33.0 39.0 10-11 36.72545746845928 39.0 37.0 39.0 33.0 39.0 12-13 36.598773997889126 39.0 37.0 39.0 32.0 39.0 14-15 37.70694897818313 40.0 37.5 41.0 32.0 41.0 16-17 37.60726561003959 40.0 37.0 41.0 32.0 41.0 18-19 37.53836678527372 40.0 37.0 41.0 32.0 41.0 20-21 37.43556074121246 40.0 37.0 41.0 32.0 41.0 22-23 37.34288112722722 40.0 37.0 41.0 32.0 41.0 24-25 37.37754051418163 40.0 37.0 41.0 32.0 41.0 26-27 37.284636452298145 40.0 37.0 41.0 31.5 41.0 28-29 37.15521819096775 40.0 37.0 41.0 31.0 41.0 30-31 36.907231319388465 39.0 36.0 41.0 30.5 41.0 32-33 36.741225378644494 39.0 36.0 41.0 30.0 41.0 34-35 36.72655298796265 39.0 36.0 41.0 30.0 41.0 36-37 36.58405930055979 39.0 36.0 41.0 30.0 41.0 38-39 36.427932184670745 39.0 35.0 41.0 30.0 41.0 40-41 36.18551331323397 39.0 35.0 41.0 29.5 41.0 42-43 35.90454774193836 39.0 35.0 41.0 28.5 41.0 44-45 35.64796905825403 39.0 35.0 40.0 27.5 41.0 46-47 35.281548067031544 39.0 35.0 40.0 26.0 41.0 48-49 34.934001629919265 38.5 34.5 40.0 24.0 41.0 50-51 34.04239571411394 37.5 33.5 39.5 23.5 40.5 52-53 34.14414720931993 38.0 33.5 39.5 23.5 40.5 54-55 34.41512529447653 38.0 34.0 40.0 23.0 41.0 56-57 34.165875458136455 38.0 34.0 40.0 22.5 41.0 58-59 33.90061500505453 38.0 33.5 40.0 21.5 41.0 60-61 33.559884836852206 37.5 33.0 40.0 19.5 41.0 62-63 33.17042943473865 37.0 32.5 40.0 18.5 41.0 64-65 32.757008430156176 36.5 32.0 39.5 16.0 41.0 66-67 32.29295260319482 36.0 31.5 39.0 12.5 40.5 68-69 31.758012211034462 35.5 31.0 39.0 8.0 40.0 70-71 31.285882049066807 35.0 30.5 38.0 7.0 40.0 72-73 30.799959029351907 35.0 30.0 37.5 3.5 39.5 74-75 30.239878691254994 34.5 29.5 37.0 2.0 39.0 76-77 29.72577320964948 34.0 29.0 36.0 2.0 39.0 78-79 29.142014063620294 34.0 28.5 36.0 2.0 38.5 80-81 28.56290998481414 34.0 27.5 35.0 2.0 37.0 82-83 28.03731758041603 34.0 26.5 35.0 2.0 37.0 84-85 27.48463288963309 33.0 26.0 35.0 2.0 36.0 86-87 26.92137509964329 33.0 25.5 35.0 2.0 36.0 88-89 26.418113479788556 32.0 24.5 35.0 2.0 35.5 90-91 25.82398475179358 32.0 23.0 35.0 2.0 35.0 92-93 25.153055653281438 32.0 19.5 34.0 2.0 35.0 94-95 24.379007441516627 31.0 14.0 34.0 2.0 35.0 96-97 23.506852340893605 31.0 2.0 34.0 2.0 35.0 98-99 22.417410298773998 30.5 2.0 34.0 2.0 35.0 100-101 19.658700696055686 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3756369116583578 1101 2 0.4205614096099879 1101 3 0.5298067739066532 1101 4 0.3721367088844758 1101 5 0.17928989850734922 1101 6 0.29396866804182764 1101 7 0.3396177720353464 1101 8 0.4219998823695832 1101 9 0.3448673914025733 1101 10-11 0.5379139104295803 1101 12-13 0.6288540011609314 1101 14-15 0.7115388897683417 1101 16-17 0.7289060849160194 1101 18-19 0.7251475623467272 1101 20-21 0.6697209633865739 1101 22-23 0.5509999791265017 1101 24-25 0.6344511654162517 1101 26-27 0.5463379704198346 1101 28-29 0.6053753148965839 1101 30-31 0.6194918979200423 1101 32-33 0.6811854254724494 1101 34-35 0.5576072107802545 1101 36-37 0.5346120690960632 1101 38-39 0.4535084929980471 1101 40-41 0.7025646191599861 1101 42-43 0.7287639181541365 1101 44-45 0.7817859578168083 1101 46-47 0.6702697944003333 1101 48-49 0.8415152156636765 1101 50-51 0.772696051807813 1101 52-53 0.704478255089036 1101 54-55 0.800264147006871 1101 56-57 0.5830928717346637 1101 58-59 0.5418622185855568 1101 60-61 0.7231256563906712 1101 62-63 0.6893209893205494 1101 64-65 0.7799449883057221 1101 66-67 0.7925491768424209 1101 68-69 0.5739944670585402 1101 70-71 0.5762926945450708 1101 72-73 0.4983768623122451 1101 74-75 0.7082405693473888 1101 76-77 0.49870057304877946 1101 78-79 0.6636829556248927 1101 80-81 1.0420441156494782 1101 82-83 0.8808331529529809 1101 84-85 0.5392264967866076 1101 86-87 0.840702246039907 1101 88-89 0.9278097520619646 1101 90-91 1.051238144883751 1101 92-93 1.1032664343319816 1101 94-95 1.3133926283315667 1101 96-97 1.5291294858745346 1101 98-99 1.993293970494868 1101 100-101 2.190226347631274 1102 1 0.13695630540814108 1102 2 0.20135972017417458 1102 3 0.15547246723581765 1102 4 -0.00660629525221168 1102 5 0.06146565545106597 1102 6 0.19910736863626255 1102 7 0.04064238356939853 1102 8 0.028217075681368442 1102 9 0.17997532757944157 1102 10-11 0.0794202371233581 1102 12-13 0.21108928812168415 1102 14-15 0.25438007932581996 1102 16-17 0.29685677494278906 1102 18-19 0.3499176982663599 1102 20-21 0.408851303707074 1102 22-23 0.4329928007623991 1102 24-25 0.3362305480421668 1102 26-27 0.1254994227784323 1102 28-29 0.3363152808297727 1102 30-31 0.33920850027691074 1102 32-33 0.3099762274330331 1102 34-35 0.3708732795395875 1102 36-37 0.42769355135003906 1102 38-39 0.47028770181371016 1102 40-41 0.5057262279478323 1102 42-43 0.35827168747314886 1102 44-45 0.2501155542293674 1102 46-47 0.41061159229375477 1102 48-49 0.4962015750337869 1102 50-51 0.48775714611726784 1102 52-53 0.282985746311617 1102 54-55 0.32700224969397595 1102 56-57 0.2687978461845475 1102 58-59 0.3928173060121196 1102 60-61 0.38710174009769815 1102 62-63 0.6225117982335462 1102 64-65 0.6845183710011682 1102 66-67 0.7551265224119916 1102 68-69 0.8241930452905493 1102 70-71 0.8190339431980398 1102 72-73 0.7722436193602533 1102 74-75 0.8398410143016761 1102 76-77 0.7140992938052015 1102 78-79 0.9882019357062362 1102 80-81 0.8320839182010147 1102 82-83 0.8351640703235788 1102 84-85 0.9875453169624997 1102 86-87 1.1933941325765645 1102 88-89 1.1631849215269803 1102 90-91 1.1299368274019166 1102 92-93 1.201264140036436 1102 94-95 1.2124444933157505 1102 96-97 1.2369800611865251 1102 98-99 0.855751821504704 1102 100-101 0.9764279849240047 1103 1 0.2358937720546308 1103 2 0.3045250181853092 1103 3 0.1861553081983125 1103 4 0.12723467520553555 1103 5 0.15377941342604373 1103 6 0.11277055990022689 1103 7 0.15729404945283676 1103 8 0.20906598726170245 1103 9 0.19813477130261248 1103 10-11 0.25573665541423196 1103 12-13 0.35754047451106175 1103 14-15 0.32074500457412825 1103 16-17 0.3926631931540001 1103 18-19 0.3020447562277937 1103 20-21 0.4125172030770443 1103 22-23 0.3933373674256231 1103 24-25 0.3516663539824805 1103 26-27 0.24428690814271903 1103 28-29 0.45302150708405975 1103 30-31 0.43439802787171544 1103 32-33 0.3540498560561218 1103 34-35 0.5012869029057683 1103 36-37 0.30986266460461565 1103 38-39 0.4858613025592362 1103 40-41 0.4677999999412421 1103 42-43 0.3716859719357899 1103 44-45 0.30032503419336365 1103 46-47 0.5987855323490336 1103 48-49 0.2832181980256081 1103 50-51 0.21196880849609556 1103 52-53 0.47884075113045554 1103 54-55 0.37765100038095767 1103 56-57 0.3815717432287329 1103 58-59 0.4807503042837169 1103 60-61 0.5625276694161343 1103 62-63 0.6109031832704588 1103 64-65 0.6103911378093443 1103 66-67 0.7349268394196926 1103 68-69 0.6115226934757203 1103 70-71 0.8436717340207416 1103 72-73 0.5777705451023927 1103 74-75 0.5475806219449062 1103 76-77 0.6207333569996507 1103 78-79 0.6679504800992646 1103 80-81 0.8011505774336634 1103 82-83 0.7561025087022202 1103 84-85 0.7113069558445311 1103 86-87 0.9970413141550694 1103 88-89 1.0113143371336335 1103 90-91 1.006366747194189 1103 92-93 0.9438112730283343 1103 94-95 1.1730849603843865 1103 96-97 1.3276092179301067 1103 98-99 1.4827727456060558 1103 100-101 1.3482951443597635 1104 1 0.12524189817953157 1104 2 0.22981826833381902 1104 3 0.14652922615033148 1104 4 0.20216160279513673 1104 5 0.15957791976390467 1104 6 0.08749592880876378 1104 7 0.17885373535229832 1104 8 0.23486668059992866 1104 9 0.018270247108176818 1104 10-11 0.154772602015953 1104 12-13 0.28310192774324605 1104 14-15 0.30662185872189696 1104 16-17 0.32301683702168305 1104 18-19 0.28016810304763595 1104 20-21 0.32929592888334724 1104 22-23 0.38271859409638154 1104 24-25 0.37473625228696505 1104 26-27 0.39419248292482933 1104 28-29 0.4388924504200631 1104 30-31 0.3843194140752857 1104 32-33 0.44982200017353335 1104 34-35 0.34414353912190876 1104 36-37 0.34541028653728034 1104 38-39 0.46712033501845696 1104 40-41 0.5149283798172917 1104 42-43 0.3633458165405514 1104 44-45 0.6242360410114856 1104 46-47 0.5456059589323203 1104 48-49 0.4986007900938958 1104 50-51 0.42598304764162975 1104 52-53 0.2810834608829964 1104 54-55 0.4122883573023728 1104 56-57 0.47663754534066527 1104 58-59 0.47760723012962103 1104 60-61 0.602354719063996 1104 62-63 0.6718081876592379 1104 64-65 0.5915179158554125 1104 66-67 0.5476464630191984 1104 68-69 0.7038547451480355 1104 70-71 0.3590102905414092 1104 72-73 0.620642401658813 1104 74-75 0.7619849746666887 1104 76-77 0.553887986790734 1104 78-79 0.5259062849597242 1104 80-81 0.3996764209803878 1104 82-83 0.2920897472596238 1104 84-85 0.34291044085914635 1104 86-87 0.6392332795886446 1104 88-89 0.6469962822635686 1104 90-91 0.4829717542428327 1104 92-93 0.6290664575491007 1104 94-95 0.7523977103008974 1104 96-97 0.7062898065287406 1104 98-99 0.49632993123213964 1104 100-101 0.42434438733254964 1105 1 0.19092945966973573 1105 2 0.1932864690155398 1105 3 0.2904169955575462 1105 4 0.2549811637167778 1105 5 0.23724439969545585 1105 6 0.18684876620707058 1105 7 0.1677652925838231 1105 8 0.25588831678975055 1105 9 0.260404378664461 1105 10-11 0.35713842447265876 1105 12-13 0.3372822859959541 1105 14-15 0.41571139960434067 1105 16-17 0.42190353339874775 1105 18-19 0.3879114788283644 1105 20-21 0.5199824452143176 1105 22-23 0.4969482281234221 1105 24-25 0.39459636817421995 1105 26-27 0.3050808125373905 1105 28-29 0.3320123761719529 1105 30-31 0.4847459783999639 1105 32-33 0.43160905524923976 1105 34-35 0.5368785675641661 1105 36-37 0.561885061967395 1105 38-39 0.5175569328718339 1105 40-41 0.5154955528984573 1105 42-43 0.5079408103671383 1105 44-45 0.6277867953377481 1105 46-47 0.6340640564080502 1105 48-49 0.5555724202914618 1105 50-51 0.47949798784458153 1105 52-53 0.5449840787811411 1105 54-55 0.5938980140163324 1105 56-57 0.587032141728038 1105 58-59 0.576195707736737 1105 60-61 0.7809146011142971 1105 62-63 0.7886353907852452 1105 64-65 0.7048919128509183 1105 66-67 0.8448674542760912 1105 68-69 0.8070711878719123 1105 70-71 1.0053541841637035 1105 72-73 0.8044761572364791 1105 74-75 0.8860774280595507 1105 76-77 0.8201771419397446 1105 78-79 0.6862643704509637 1105 80-81 0.8533535698974646 1105 82-83 0.9633167920788388 1105 84-85 0.8704441158898746 1105 86-87 1.0590200949744997 1105 88-89 0.9921406117869473 1105 90-91 1.1978481328078736 1105 92-93 0.8616903367900086 1105 94-95 1.223850927579747 1105 96-97 1.3734065884416324 1105 98-99 1.7465710146302733 1105 100-101 1.9532493112867613 1106 1 0.04619185756362043 1106 2 -0.0067852750648143 1106 3 -0.033121446805509436 1106 4 0.05954086482363863 1106 5 0.019534555860431624 1106 6 0.039711081412868054 1106 7 0.030037938329186886 1106 8 0.26140744297116925 1106 9 0.1932719834761798 1106 10-11 -0.10923837786935309 1106 12-13 -0.1276132746870644 1106 14-15 -0.013558361177793188 1106 16-17 -0.005808985639276898 1106 18-19 0.1001044764845247 1106 20-21 0.02098944634215627 1106 22-23 0.07521159577839143 1106 24-25 -0.09925446493870993 1106 26-27 0.08884613035454691 1106 28-29 0.04965913993632398 1106 30-31 0.25420732019666303 1106 32-33 0.22788170707461575 1106 34-35 0.23599499057456086 1106 36-37 0.3144538648444595 1106 38-39 0.26999349560078656 1106 40-41 0.40917979382479785 1106 42-43 0.6186364543462659 1106 44-45 0.34428595781680826 1106 46-47 0.527218576741113 1106 48-49 0.4060886128696026 1106 50-51 0.4828891116426419 1106 52-53 0.3164434628156485 1106 54-55 0.057954533759641436 1106 56-57 -0.05930678217155361 1106 58-59 0.06579509608805267 1106 60-61 0.18743297641487544 1106 62-63 0.2520223497786347 1106 64-65 0.18712309294984664 1106 66-67 0.22371115858104673 1106 68-69 0.08702662142199813 1106 70-71 -0.2839728198825391 1106 72-73 0.17120392797158246 1106 74-75 0.12367269416625248 1106 76-77 0.18874791473901098 1106 78-79 0.07839668963916324 1106 80-81 -0.11245475180785647 1106 82-83 -0.20717191886851793 1106 84-85 0.09830073108928516 1106 86-87 0.11252567490075194 1106 88-89 0.2513784221332962 1106 90-91 0.10550346938184774 1106 92-93 0.09473015779461136 1106 94-95 0.2133226357880602 1106 96-97 0.49822988824373127 1106 98-99 0.5889779981260972 1106 100-101 0.5490322185636174 1107 1 0.2109810252326625 1107 2 0.17780701274699595 1107 3 0.05254007618047041 1107 4 0.20970190151819423 1107 5 0.16113625742595872 1107 6 0.08734037282111018 1107 7 0.2455316983466389 1107 8 0.14058447426046428 1107 9 0.1372636332009165 1107 10-11 0.20866441838210648 1107 12-13 0.22308162213128213 1107 14-15 0.258730753468825 1107 16-17 0.3249244812095853 1107 18-19 0.3580669305443749 1107 20-21 0.3827932997093839 1107 22-23 0.42065272724675395 1107 24-25 0.5150588642942182 1107 26-27 0.32683049025262534 1107 28-29 0.3190615620009254 1107 30-31 0.5556087787679687 1107 32-33 0.5820230618431452 1107 34-35 0.622785285348975 1107 36-37 0.5008655794686447 1107 38-39 0.6095332306098342 1107 40-41 0.6458454101423712 1107 42-43 0.6284319695576457 1107 44-45 0.5738352065647234 1107 46-47 0.746615545070064 1107 48-49 0.7020749664110468 1107 50-51 0.6809483392118807 1107 52-53 0.6031190776486 1107 54-55 0.6835078606898861 1107 56-57 0.8707573901180155 1107 58-59 0.7406350440957681 1107 60-61 0.6418791344095993 1107 62-63 0.8859115601686085 1107 64-65 0.8143345070126102 1107 66-67 0.822221531283283 1107 68-69 0.975866611847664 1107 70-71 0.7301432659107796 1107 72-73 0.8850403583198023 1107 74-75 0.8805853041120457 1107 76-77 1.0173476075119012 1107 78-79 1.064081391001686 1107 80-81 1.1399479217345565 1107 82-83 1.0658564799449906 1107 84-85 1.2919401603861438 1107 86-87 1.1457964922642319 1107 88-89 1.176880393052766 1107 90-91 1.1685153251971947 1107 92-93 1.397118591027251 1107 94-95 1.5820784871919429 1107 96-97 1.5412386536088718 1107 98-99 1.7756654293863008 1107 100-101 1.818130897850633 1108 1 0.03758956198310415 1108 2 -0.02811473746732318 1108 3 0.042901263745331875 1108 4 0.057017810916740075 1108 5 0.1894774116018354 1108 6 0.17378089305753974 1108 7 0.17586462935778968 1108 8 0.19113747926881075 1108 9 0.21167678800848222 1108 10-11 0.33258576251422056 1108 12-13 0.2507375125793203 1108 14-15 0.25853828742180696 1108 16-17 0.3880794297725103 1108 18-19 0.3645875110163317 1108 20-21 0.3621543124332902 1108 22-23 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-0.1758612076615229 2316 74-75 -0.08485932159492648 2316 76-77 -0.21234446482665703 2316 78-79 -0.18367865901831237 2316 80-81 0.1802820971980985 2316 82-83 0.008554068448013652 2316 84-85 -0.3259012038647917 2316 86-87 -0.5493576619979379 2316 88-89 -0.5623253173517746 2316 90-91 -0.6034874424066068 2316 92-93 -0.8379087358864261 2316 94-95 -0.9042953487957917 2316 96-97 -0.7924488293316436 2316 98-99 -0.9493475834297342 2316 100-101 -0.7761223569403057 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5542.0 3 1252.0 4 1205.0 5 1250.0 6 1471.0 7 1794.0 8 2123.0 9 2509.0 10 3139.0 11 4030.0 12 5176.0 13 6131.0 14 7000.0 15 8528.0 16 10252.0 17 13165.0 18 11637.0 19 8946.0 20 8907.0 21 9555.0 22 10421.0 23 11405.0 24 12589.0 25 14188.0 26 16348.0 27 18783.0 28 22282.0 29 26401.0 30 31871.0 31 38684.0 32 48523.0 33 61824.0 34 82084.0 35 111668.0 36 155589.0 37 199526.0 38 134549.0 39 12406.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.649206698080675 19.0518438305788 14.858574433145172 22.440375038195356 2 14.976153049194386 19.995355650030366 41.50584116323104 23.52265013754421 3 19.487977821422795 24.595516947873378 29.695464341631546 26.221040889072285 4 12.394028059119213 16.690067128959768 37.321236544734184 33.59466826718683 5 13.430620030745622 37.590854562133934 34.18677295135635 14.79175245576409 6 31.533791495881978 36.15379054992892 17.71770083431275 14.59471711987635 7 28.31069992084181 33.04528794573336 20.38577794119091 18.258234192233928 8 26.12069434813611 35.75334238259828 19.37711785373568 18.74884541552993 9 26.16566300541513 17.34965606605245 20.429622939876705 36.05505798865571 10-11 24.82655832000196 27.200055333551994 26.868946498256324 21.104439848189724 12-13 25.62191596476676 24.45369156145047 27.89090028061123 22.03349219317154 14-15 22.708589520952206 25.817376079557526 26.303589690504538 25.17044470898574 16-17 21.69546237266384 29.212183575581978 26.94598032946649 22.14637372228769 18-19 21.58746321081874 27.511837683187366 29.666180011315628 21.234519094678266 20-21 22.44195488453439 26.445818610129564 30.02346789449263 21.088758610843417 22-23 22.14114581706724 26.414960492912815 29.885204138540267 21.558689551479677 24-25 21.66360371623114 26.707098504753706 30.051536985405363 21.577760793609787 26-27 21.542705427335346 26.89009120585987 29.852151299202777 21.715052067602 28-29 21.530857300435777 27.02036270368728 29.954155581872488 21.494624414004456 30-31 21.704729325099937 26.75574777527785 29.939436507844423 21.600086391777786 32-33 21.4487290018903 26.820862540139835 29.971850796727768 21.7585576612421 34-35 21.76211351523092 26.938581898536707 29.794088982975214 21.505215603257163 36-37 21.86855348156608 26.657446732574325 29.812984046539846 21.661015739319748 38-39 21.530609786888185 27.08229433108478 29.751611921842645 21.63548396018439 40-41 21.834020420335754 27.031226509409173 29.655570222730393 21.47918284752468 42-43 21.67453178490816 26.86403748486948 29.519111635006162 21.942319095216188 44-45 21.652416059139984 27.14931480627934 29.49712898186723 21.701140152713446 46-47 21.925839989290974 27.376645397349513 28.848333407701578 21.849181205657935 48-49 21.948982004934923 27.339960824033877 28.860282798716742 21.85077437231446 50-51 22.080380887786248 27.733012654612953 28.47970621307584 21.70690024452496 52-53 22.044289244040733 27.7725855863576 28.359853678392387 21.82327149120928 54-55 21.953938935337025 27.761430829655083 28.36807983120729 21.916550403800603 56-57 21.995329510401543 28.03805980635535 28.319582823125838 21.647027860117273 58-59 21.99119333402031 27.699333232517482 28.546097155253285 21.76337627820892 60-61 22.033648815136704 27.728468567646004 28.551088336391807 21.68679428082549 62-63 21.8947758164599 27.688618854524933 28.77271165950689 21.643893669508273 64-65 21.822784584261743 27.677847738470422 28.71583840195206 21.78352927531577 66-67 21.8231193957015 27.827920881646317 28.378949828843353 21.970009893808832 68-69 21.765071439692605 27.85128623567448 28.485805077846 21.89783724678692 70-71 21.844701771979334 27.702020900452073 28.433973896293573 22.01930343127502 72-73 21.91277978957736 27.747177012490997 28.32493373551482 22.015109462416817 74-75 21.731502356674657 28.127935806377074 28.087297331778572 22.053264505169697 76-77 21.958338891211177 27.862790383317353 28.122252196346924 22.056618529124545 78-79 22.022273308656594 27.84979993459786 28.062156120163262 22.06577063658228 80-81 21.8451892121443 28.14091535024309 27.96712201175603 22.04677342585658 82-83 21.971212630149612 28.053924015511118 27.931564088186576 22.043299266152694 84-85 22.01580650370094 27.903367825495057 28.006094933586457 22.074730737217546 86-87 21.921843296760056 28.00422661545605 28.060471466554386 22.01345862122951 88-89 22.063182442055847 27.803825132468308 27.992284967328423 22.14070745814742 90-91 21.981327472276487 27.874963922292523 28.054292990686935 22.089415614744063 92-93 21.97941013791996 27.781727835566578 28.093118275732863 22.145743750780593 94-95 21.879573708611076 27.8719377201681 28.03377535348453 22.2147132177363 96-97 21.866160885105838 27.890486753789684 27.891601923623355 22.351750437481126 98-99 21.921912946344925 27.76702182140671 27.879082034142677 22.43198319810568 100-101 22.29156334626102 27.132711567485025 28.424781555389846 22.150943530864108 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1573.0 1 911.0 2 282.0 3 353.5 4 321.0 5 295.0 6 480.5 7 972.0 8 1585.5 9 1854.5 10 1660.5 11 1436.5 12 1500.5 13 1622.0 14 1723.5 15 1828.5 16 1871.5 17 1900.5 18 2004.0 19 2179.5 20 2399.5 21 2751.5 22 3263.5 23 3957.5 24 4809.5 25 5897.5 26 7472.5 27 9291.0 28 10667.0 29 12102.5 30 13914.0 31 15737.5 32 17784.0 33 19860.0 34 22318.5 35 24661.5 36 27549.0 37 30600.0 38 32219.0 39 33496.0 40 34785.0 41 36631.5 42 38071.0 43 39260.0 44 40710.5 45 41666.0 46 42675.0 47 43724.0 48 44336.0 49 44016.0 50 42486.0 51 40358.5 52 38372.5 53 37036.5 54 35960.5 55 32842.0 56 28581.0 57 25087.0 58 22440.0 59 19666.5 60 16205.5 61 13029.0 62 10176.5 63 7602.5 64 5671.5 65 4209.0 66 3046.5 67 2229.5 68 1602.0 69 1149.0 70 823.0 71 588.0 72 422.5 73 285.0 74 194.0 75 142.5 76 104.0 77 75.0 78 49.0 79 34.5 80 26.0 81 16.0 82 10.5 83 7.0 84 5.0 85 7.0 86 7.5 87 3.5 88 2.0 89 2.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31253479165089443 2 0.2774425408927148 3 0.2775316075190046 4 0.277798807397874 5 0.28911026893667807 6 0.19523404482723303 7 0.19719351060560852 8 0.19790604361592687 9 0.1951449782009432 10-11 0.20284924137501056 12-13 0.1928737792305534 14-15 0.19211671290709015 16-17 0.2000881759600269 18-19 0.19425431193804524 20-21 0.18481324955132686 22-23 0.1843679164198779 24-25 0.18735164840058605 26-27 0.1824529839546473 28-29 0.19826231012108606 30-31 0.20289377468815545 32-33 0.2045860405876616 34-35 0.2022703083041269 36-37 0.1776879194481432 38-39 0.16815779043513499 40-41 0.17461512084114522 42-43 0.18610471563252892 44-45 0.19233937947281463 46-47 0.19639191096900038 48-49 0.1932745790488575 50-51 0.19728257723189832 52-53 0.2211524330775637 54-55 0.18619378225881872 56-57 0.1681132571219901 58-59 0.16918205663746766 60-61 0.16245752635258803 62-63 0.1701172562135105 64-65 0.1681132571219901 66-67 0.16495139188870234 68-69 0.17675271987210034 70-71 0.17082978922382888 72-73 0.16637645790933908 74-75 0.16887032344545336 76-77 0.17577298698291255 78-79 0.17782151938757787 80-81 0.16775699061683094 82-83 0.16757885736425135 84-85 0.16245752635258803 86-87 0.15706899546205538 88-89 0.16268019291831254 90-91 0.17016178952665542 92-93 0.1616559267159799 94-95 0.16731165748538193 96-97 0.16472872532297786 98-99 0.17180952211301664 100-101 0.14669273349929415 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1122755.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.50876646002048 #Duplication Level Percentage of deduplicated Percentage of total 1 83.57854679525389 56.422845966686886 2 10.125349881737842 13.67099760984472 3 2.746324411147306 5.562029198867903 4 1.1742248283104002 3.170818788238579 5 0.60941210608706 2.0570329773870286 6 0.3881445447333672 1.5721895653881517 7 0.2521872986278953 1.1917397413077822 8 0.1760798973695944 0.9509549335862659 9 0.13598141696967606 0.8261943948997676 >10 0.7493012723387952 9.10250432845549 >50 0.0462394998165205 2.1223187060562902 >100 0.01741639553895791 1.9468595984384398 >500 1.3194201145059288E-4 0.06502286979216901 >1k 5.277680458023715E-4 0.8730879037025213 >5k 1.3194201145059288E-4 0.46540341734805896 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5225 0.4653731223641845 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4326 0.38530222532965785 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2871 0.25571028407800456 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1474 0.13128420715115943 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1131 0.10073435433375937 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.7813325257959217E-4 14-15 0.0 0.0 0.0 0.0 1.7813325257959217E-4 16-17 0.0 0.0 0.0 4.453331314489804E-5 1.7813325257959217E-4 18-19 0.0 0.0 0.0 8.906662628979608E-5 1.7813325257959217E-4 20-21 0.0 0.0 0.0 1.7813325257959217E-4 1.7813325257959217E-4 22-23 0.0 0.0 0.0 4.007998183040824E-4 2.6719987886938826E-4 24-25 0.0 0.0 0.0 9.351995760428589E-4 2.6719987886938826E-4 26-27 0.0 0.0 0.0 0.0012914660812020431 2.6719987886938826E-4 28-29 2.226665657244902E-4 0.0 0.0 0.0017813325257959216 2.6719987886938826E-4 30-31 3.5626650515918433E-4 0.0 0.0 0.0036071983647367415 2.6719987886938826E-4 32-33 3.5626650515918433E-4 0.0 0.0 0.006279197153430624 2.6719987886938826E-4 34-35 3.5626650515918433E-4 0.0 0.0 0.008594929436965323 2.6719987886938826E-4 36-37 4.453331314489804E-4 0.0 0.0 0.012469327680571452 2.6719987886938826E-4 38-39 4.453331314489804E-4 0.0 0.0 0.017412525439655133 2.6719987886938826E-4 40-41 4.453331314489804E-4 0.0 0.0 0.026942654452663313 2.6719987886938826E-4 42-43 4.453331314489804E-4 0.0 0.0 0.035715717142208225 2.6719987886938826E-4 44-45 6.234663840285725E-4 0.0 0.0 0.04453331314489804 2.6719987886938826E-4 46-47 6.234663840285725E-4 0.0 0.0 0.061010639008510316 2.6719987886938826E-4 48-49 6.234663840285725E-4 0.0 0.0 0.07339090006279197 2.6719987886938826E-4 50-51 6.234663840285725E-4 0.0 0.0 0.08750796032972466 2.6719987886938826E-4 52-53 6.234663840285725E-4 0.0 0.0 0.10189222047552672 2.6719987886938826E-4 54-55 6.234663840285725E-4 0.0 0.0 0.11814687977341451 2.6719987886938826E-4 56-57 6.679996971734706E-4 0.0 0.0 0.15746979528035948 2.6719987886938826E-4 58-59 7.125330103183687E-4 0.0 0.0 0.193051912483133 2.6719987886938826E-4 60-61 7.125330103183687E-4 0.0 0.0 0.2225774990982004 2.6719987886938826E-4 62-63 7.125330103183687E-4 0.0 0.0 0.25829321624040863 2.6719987886938826E-4 64-65 7.125330103183687E-4 0.0 0.0 0.2977051983736434 2.6719987886938826E-4 66-67 7.125330103183687E-4 0.0 0.0 0.3594283703924721 2.6719987886938826E-4 68-69 7.125330103183687E-4 0.0 0.0 0.42881127227222327 2.6719987886938826E-4 70-71 7.125330103183687E-4 0.0 0.0 0.47468058481146824 2.6719987886938826E-4 72-73 7.125330103183687E-4 0.0 0.0 0.5250477619783479 3.117331920142863E-4 74-75 7.570663234632667E-4 0.0 0.0 0.6063655917809317 4.0079981830408234E-4 76-77 8.015996366081648E-4 0.0 0.0 0.7088367453273421 4.453331314489804E-4 78-79 8.015996366081648E-4 0.0 0.0 0.811085232308028 4.453331314489804E-4 80-81 8.906662628979608E-4 0.0 0.0 0.8952977274650302 4.898664445938785E-4 82-83 8.906662628979608E-4 0.0 0.0 1.0068536768929999 5.343997577387765E-4 84-85 8.906662628979608E-4 0.0 0.0 1.1875698616349961 5.343997577387765E-4 86-87 8.906662628979608E-4 0.0 0.0 1.3603591166372007 5.343997577387765E-4 88-89 8.906662628979608E-4 0.0 0.0 1.5760784855110868 5.343997577387765E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2970 0.0 51.564037 1 GCGTACC 45 9.5794816E-4 42.262882 2 GTATCAA 5030 0.0 39.807198 1 ATCAACG 6390 0.0 30.877306 3 TCAACGC 6405 0.0 30.73214 4 TATCAAC 6470 0.0 30.49688 2 CAACGCA 6575 0.0 29.866568 5 AACGCAG 6735 0.0 29.131004 6 GCGCGTT 35 0.008392242 27.14352 12-13 TACATGG 5030 0.0 25.616095 2 GTACATG 5235 0.0 25.34749 1 CGCAGAG 7685 0.0 25.28377 8 ACGCAGA 7780 0.0 25.158224 7 GTACCTG 995 0.0 24.8558 1 TACCTGG 960 0.0 24.763407 2 ACATGGG 5160 0.0 23.58753 3 GCAGAGT 8175 0.0 23.012817 9 GTACCGG 125 2.2364555E-4 22.829096 1 GAGTACT 4765 0.0 22.529406 12-13 TACACGG 275 5.0931703E-11 22.477173 5 >>END_MODULE