FastQCFastQC Report
Thu 26 May 2016
SRR937635_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937635_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122755
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67590.6020013270927317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62700.5584477468370214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41020.36535130104074354No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16740.14909753240911866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35350.045.842721
GTATCAA57050.039.151431
TAATCCG651.3250545E-436.553035
CCGTAAG902.4048839E-531.6821121
ATCAACG72450.030.6298713
TCAACGC73500.030.2569564
TATCAAC73850.030.17792
CAACGCA74500.029.8508225
AACGCAG76950.029.1331396
CGCAGAG87300.025.4615888
GGACCGA3200.025.2320676
ACGCAGA89050.025.121237
ATCGGAC1201.7118426E-423.747838
GCAGAGT91650.023.6312279
GAGTACT55150.022.60672812-13
GTGTAGG5050.022.5852681
AGGACCG4050.022.2928335
ACCGAGT3850.022.2057638
GTACTTT56250.022.12242114-15
TGGTATC17550.021.6610532
Produced by FastQC (version 0.11.5)