##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937635_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1122755 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14896482313595 33.0 31.0 34.0 31.0 34.0 2 32.43127218315661 34.0 31.0 34.0 31.0 34.0 3 32.52041273474623 34.0 31.0 34.0 31.0 34.0 4 36.15411821813307 37.0 35.0 37.0 35.0 37.0 5 36.070403605417034 37.0 35.0 37.0 35.0 37.0 6 36.114957403885974 37.0 35.0 37.0 35.0 37.0 7 36.06484495727029 37.0 35.0 37.0 35.0 37.0 8 36.03855248918954 37.0 35.0 37.0 35.0 37.0 9 37.73829464130643 39.0 37.0 39.0 35.0 39.0 10-11 37.65358559970786 39.0 37.0 39.0 35.0 39.0 12-13 37.547389234516885 39.0 37.0 39.0 35.0 39.0 14-15 38.763477339223606 40.0 38.0 41.0 35.0 41.0 16-17 38.712377143722364 40.0 38.0 41.0 34.5 41.0 18-19 38.700762855654176 40.0 38.0 41.0 34.5 41.0 20-21 38.60761296988212 40.0 38.0 41.0 34.0 41.0 22-23 38.55541413754559 40.0 38.0 41.0 34.0 41.0 24-25 38.5874099870408 40.0 38.0 41.0 34.0 41.0 26-27 38.45695231818162 40.0 38.0 41.0 34.0 41.0 28-29 38.30573811739872 40.0 38.0 41.0 34.0 41.0 30-31 38.33071239941037 40.0 38.0 41.0 34.0 41.0 32-33 38.23816683069771 40.0 38.0 41.0 34.0 41.0 34-35 38.09393723474845 40.0 37.5 41.0 34.0 41.0 36-37 37.94049636830832 40.0 37.0 41.0 33.0 41.0 38-39 37.81282915685078 40.0 37.0 41.0 33.0 41.0 40-41 37.62546860178757 40.0 37.0 41.0 33.0 41.0 42-43 37.39685906542389 40.0 37.0 41.0 33.0 41.0 44-45 37.08903367163807 40.0 36.5 41.0 31.5 41.0 46-47 36.69979781875833 40.0 36.0 41.0 31.0 41.0 48-49 36.339192878232566 39.0 36.0 41.0 30.0 41.0 50-51 36.06511126648289 39.0 35.5 41.0 29.5 41.0 52-53 35.824251506339316 39.0 35.0 40.0 29.0 41.0 54-55 35.59520732483935 39.0 35.0 40.0 28.0 41.0 56-57 35.31026270201424 38.0 35.0 40.0 28.0 41.0 58-59 35.0468868987446 38.0 34.5 40.0 27.0 41.0 60-61 34.713131983380165 38.0 34.0 40.0 26.0 41.0 62-63 34.32910029347453 37.5 34.0 40.0 26.0 41.0 64-65 33.90669691963073 37.0 33.0 39.5 25.5 41.0 66-67 33.46947196850604 36.0 33.0 39.0 24.5 40.0 68-69 33.0091377905242 36.0 32.0 39.0 22.5 40.0 70-71 32.59964818682616 35.0 32.0 38.0 22.0 40.0 72-73 32.09320822441227 35.0 31.0 37.5 20.5 39.5 74-75 31.600198173243495 35.0 31.0 37.0 19.5 39.0 76-77 30.392626174009468 33.5 29.5 35.5 19.0 38.0 78-79 30.981008323276228 34.0 31.0 36.0 19.0 38.5 80-81 30.785491937243656 34.0 31.0 36.0 18.0 37.5 82-83 30.40091872225018 34.0 30.5 35.0 16.0 37.0 84-85 29.996922748061685 34.0 30.0 35.0 10.0 36.5 86-87 29.59233804347342 34.0 30.0 35.0 7.0 36.0 88-89 29.124441218253313 34.0 29.0 35.0 2.0 36.0 90-91 28.545842147218224 34.0 29.0 35.0 2.0 35.0 92-93 28.062320809081235 33.0 27.5 35.0 2.0 35.0 94-95 27.54575931525578 33.0 27.0 35.0 2.0 35.0 96-97 26.92335816807763 33.0 25.5 34.5 2.0 35.0 98-99 25.956493625056225 32.0 24.5 34.0 2.0 35.0 100-101 23.08689607260711 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38780657729267176 1101 2 0.33275020442373204 1101 3 0.3114968382411831 1101 4 0.18884067485036837 1101 5 0.03262710734971108 1101 6 0.06627058650987294 1101 7 0.2146806011114677 1101 8 0.21330893487547797 1101 9 0.22141457156048716 1101 10-11 0.27266542747499756 1101 12-13 0.2601122608911268 1101 14-15 0.28640231663145244 1101 16-17 0.26010764258363395 1101 18-19 0.24310272600665428 1101 20-21 0.36805612351292183 1101 22-23 0.16883479748918262 1101 24-25 0.1680458278096424 1101 26-27 -0.005834754747361615 1101 28-29 0.19630877107164224 1101 30-31 0.20160421778350468 1101 32-33 0.22445017391799382 1101 34-35 0.27333461775953793 1101 36-37 0.2593716662598169 1101 38-39 0.38870020983993925 1101 40-41 0.5000228880330866 1101 42-43 0.3289602846970823 1101 44-45 0.33991547396770017 1101 46-47 0.25178199482665775 1101 48-49 0.27357137820083466 1101 50-51 0.2994232514657753 1101 52-53 0.5041443911542203 1101 54-55 0.3919014996564556 1101 56-57 0.3227702499419962 1101 58-59 0.3058419202309963 1101 60-61 0.5387764319194233 1101 62-63 0.47826952831854896 1101 64-65 0.3976849320687137 1101 66-67 0.1836334522891505 1101 68-69 0.13609127080137995 1101 70-71 0.19271023202016835 1101 72-73 0.13881874654165216 1101 74-75 0.0032442088497859345 1101 76-77 0.12744003277225957 1101 78-79 0.2592361771261551 1101 80-81 0.27480456420622446 1101 82-83 0.2850699897935556 1101 84-85 0.30087850605995925 1101 86-87 0.5047942561226755 1101 88-89 0.5361472934367875 1101 90-91 0.41127388758905425 1101 92-93 0.24054387687137435 1101 94-95 0.14908191634956935 1101 96-97 0.09754084561270204 1101 98-99 0.2622388207669246 1101 100-101 0.49800974872599113 1102 1 0.27336555111683936 1102 2 0.2267998098744215 1102 3 0.15902525622011154 1102 4 0.09303124042897792 1102 5 -0.01799600246361166 1102 6 0.1090678889745007 1102 7 0.09244852964068428 1102 8 0.09432277017286594 1102 9 0.04255685264645592 1102 10-11 0.17095209275117895 1102 12-13 0.22776834251243372 1102 14-15 0.2698790434364753 1102 16-17 0.25947280057717137 1102 18-19 0.14054423885123413 1102 20-21 0.18731212865770885 1102 22-23 0.22078234155008403 1102 24-25 0.21466999786561303 1102 26-27 0.15280491918457528 1102 28-29 0.11804504875092903 1102 30-31 0.20476495752000545 1102 32-33 0.345020184694242 1102 34-35 0.366372212920659 1102 36-37 0.35224414166567186 1102 38-39 0.2676304945411587 1102 40-41 0.31493320193443 1102 42-43 0.4159731814190124 1102 44-45 0.409579498718287 1102 46-47 0.38564979473628114 1102 48-49 0.5567513239720299 1102 50-51 0.37551304185058854 1102 52-53 0.49714461710757263 1102 54-55 0.5433988751213974 1102 56-57 0.547696380577797 1102 58-59 0.6122290130804373 1102 60-61 0.544969726319259 1102 62-63 0.4419618667619005 1102 64-65 0.5987256210526226 1102 66-67 0.5098171002190739 1102 68-69 0.4899946149109482 1102 70-71 0.25713822242583717 1102 72-73 0.30169330767071756 1102 74-75 0.15872357757088906 1102 76-77 0.20848819559772025 1102 78-79 0.2851447703058412 1102 80-81 0.21290812036432527 1102 82-83 0.3170845377130469 1102 84-85 0.4795180930867602 1102 86-87 0.09725175951197684 1102 88-89 0.22299289777508946 1102 90-91 0.017066114793834686 1102 92-93 0.10669476156564528 1102 94-95 0.04255794791351164 1102 96-97 0.0867341921556175 1102 98-99 0.17076204181882204 1102 100-101 0.06772113677479297 1103 1 0.39789063693990556 1103 2 0.3180948420929184 1103 3 0.2705100151588553 1103 4 0.1652922699871624 1103 5 0.09873372059271901 1103 6 0.18411587820579456 1103 7 0.16982469352888785 1103 8 0.1863027939086166 1103 9 0.20624115120717335 1103 10-11 0.1721595894257817 1103 12-13 0.23377993957812038 1103 14-15 0.2730722190093502 1103 16-17 0.30015731419336333 1103 18-19 0.24739176451849687 1103 20-21 0.137799985988309 1103 22-23 0.28558373383517477 1103 24-25 0.26630043697703343 1103 26-27 0.19706413271873657 1103 28-29 0.1551317638979981 1103 30-31 0.2918616289391309 1103 32-33 0.365058177828395 1103 34-35 0.40580343101080274 1103 36-37 0.3565058243552883 1103 38-39 0.36533338345305566 1103 40-41 0.45717208874356885 1103 42-43 0.651343833515007 1103 44-45 0.708530107643881 1103 46-47 0.834222065323857 1103 48-49 0.625158081492998 1103 50-51 0.677170163771116 1103 52-53 0.8741058035766045 1103 54-55 0.4051420988826351 1103 56-57 0.669669474748197 1103 58-59 0.640799133113255 1103 60-61 0.7182112495492348 1103 62-63 0.6420713857544911 1103 64-65 0.5647982301534498 1103 66-67 0.6357328781225249 1103 68-69 0.6317910449900452 1103 70-71 0.6916155977932306 1103 72-73 0.6270367990565546 1103 74-75 0.3974244035432193 1103 76-77 0.45655747532117275 1103 78-79 0.7406661205356322 1103 80-81 0.5537762276371794 1103 82-83 0.44057775550052725 1103 84-85 0.506261834574147 1103 86-87 0.31637775812606606 1103 88-89 0.44347772839899235 1103 90-91 0.4853561848063386 1103 92-93 0.5933095494174019 1103 94-95 0.5872436881424257 1103 96-97 0.5608757623791938 1103 98-99 0.4674681087727599 1103 100-101 0.47663598277117813 1104 1 0.228571000927694 1104 2 0.14031092363848074 1104 3 0.10655739622593785 1104 4 0.12312010577299048 1104 5 0.07730555025494823 1104 6 -0.016340436462179753 1104 7 0.012727138536178018 1104 8 0.012530838937180988 1104 9 0.10433580103590856 1104 10-11 0.2418406715772008 1104 12-13 0.12705801667284078 1104 14-15 0.0879330341694029 1104 16-17 0.1737472161625533 1104 18-19 0.08049260592255791 1104 20-21 0.15885134207630358 1104 22-23 0.15132038352795263 1104 24-25 0.05235121676087573 1104 26-27 0.18715835105820844 1104 28-29 0.10973229646214122 1104 30-31 0.12041040643548939 1104 32-33 0.16539243423349603 1104 34-35 0.22757029086250213 1104 36-37 0.24548895785392943 1104 38-39 0.02029048573670167 1104 40-41 0.16287646540413903 1104 42-43 0.30202520234079344 1104 44-45 0.05761304519911192 1104 46-47 0.13591728613700838 1104 48-49 0.19411804460953164 1104 50-51 0.22016097541423107 1104 52-53 0.32575388566595365 1104 54-55 0.1463401613185482 1104 56-57 0.30818860952357596 1104 58-59 0.21333218193951353 1104 60-61 0.316039617308121 1104 62-63 0.19851372726346028 1104 64-65 0.07451634354055159 1104 66-67 -0.10272552306199856 1104 68-69 -0.023493368494882816 1104 70-71 -0.03436286320871318 1104 72-73 -0.03726455777028548 1104 74-75 -0.28806130263623686 1104 76-77 -0.044660890753132065 1104 78-79 0.06103211799989339 1104 80-81 0.027015027387744084 1104 82-83 0.007724577109271991 1104 84-85 -0.005176325035503027 1104 86-87 -0.182621098131861 1104 88-89 -0.1643059240689233 1104 90-91 -0.24797458717522858 1104 92-93 -0.16442474777230487 1104 94-95 -0.4311219428515045 1104 96-97 -0.2879552268247103 1104 98-99 0.13740246920815125 1104 100-101 -0.04942330858159849 1105 1 0.22029752038019978 1105 2 0.21949497478718172 1105 3 0.12666765355807286 1105 4 0.07216232184502758 1105 5 -0.044828971494119685 1105 6 0.135093382661168 1105 7 0.10043690199573518 1105 8 0.1518826172407941 1105 9 0.2231090906676485 1105 10-11 0.14905561484595609 1105 12-13 0.14342660392411943 1105 14-15 0.16443102607011895 1105 16-17 0.07206228011236959 1105 18-19 0.23351018968161696 1105 20-21 0.0031867183475640104 1105 22-23 0.05136274731583512 1105 24-25 0.11403303126879649 1105 26-27 0.0649820229395246 1105 28-29 0.013736510544205771 1105 30-31 0.15570125334892282 1105 32-33 0.03201269582988431 1105 34-35 0.11458788019574939 1105 36-37 0.021849267444032705 1105 38-39 0.16789414463016783 1105 40-41 0.3503160203613973 1105 42-43 0.19324293827504135 1105 44-45 0.1414473581950375 1105 46-47 0.138565719652334 1105 48-49 0.1852007459699152 1105 50-51 0.4021315604525668 1105 52-53 0.44778458826380785 1105 54-55 0.3863261422492599 1105 56-57 0.3476426740781804 1105 58-59 0.4224107138112956 1105 60-61 0.34602244882565714 1105 62-63 0.3713468334488539 1105 64-65 0.3161021148819998 1105 66-67 0.34819170868743043 1105 68-69 0.4800859535172819 1105 70-71 0.24362371415954698 1105 72-73 0.31140892612172166 1105 74-75 0.20055342937099851 1105 76-77 0.26047195595405626 1105 78-79 0.4345239532159084 1105 80-81 0.3855959241065001 1105 82-83 0.2600733983090038 1105 84-85 0.14812345171848307 1105 86-87 0.21805532892856405 1105 88-89 0.3998626726584753 1105 90-91 0.5626557971367916 1105 92-93 0.7458246411579275 1105 94-95 0.4588039762615317 1105 96-97 0.3359567624059707 1105 98-99 0.44822446604759136 1105 100-101 0.4749170565831058 1106 1 0.12423189278669611 1106 2 0.0720912896950523 1106 3 0.09171673441304762 1106 4 0.04050220638570323 1106 5 0.027979110050935674 1106 6 0.07000234975921416 1106 7 0.030937864156811656 1106 8 -0.10740991316684045 1106 9 -0.029791832581381072 1106 10-11 -0.15318380366218776 1106 12-13 -0.26604437468336783 1106 14-15 0.09608414952161581 1106 16-17 0.14661684361347938 1106 18-19 0.14268628795885263 1106 20-21 0.16221385645795294 1106 22-23 0.17321900379484845 1106 24-25 0.14568250671621286 1106 26-27 0.06104065852519369 1106 28-29 0.004253979923767304 1106 30-31 0.04823789298995962 1106 32-33 -0.07185130049779076 1106 34-35 -0.34594375559968427 1106 36-37 -0.08170920854146146 1106 38-39 -0.16461458777961724 1106 40-41 -0.434868219044958 1106 42-43 -0.5539868586223307 1106 44-45 -0.27974891354478615 1106 46-47 -0.25992856666125874 1106 48-49 -0.20160852725647516 1106 50-51 -0.06394884155748315 1106 52-53 -0.20323138087407955 1106 54-55 -0.17984789186564853 1106 56-57 -0.08214540728447162 1106 58-59 -0.178458119161796 1106 60-61 -0.14744154385400776 1106 62-63 -0.43031194440897735 1106 64-65 -0.4591337133821227 1106 66-67 -0.4886695485971799 1106 68-69 -0.15945400276746824 1106 70-71 -0.2746535463109865 1106 72-73 -0.1498310304670305 1106 74-75 -0.450425299725179 1106 76-77 -0.3868952490972113 1106 78-79 -0.3063765565926637 1106 80-81 -0.31638798211547225 1106 82-83 -0.45615368386533106 1106 84-85 -0.5863930509399964 1106 86-87 -0.4484144975176392 1106 88-89 -0.22445446657672008 1106 90-91 -0.3555893912978192 1106 92-93 -0.22093824161650488 1106 94-95 -0.27876772981877807 1106 96-97 -0.3386481131514891 1106 98-99 -0.5318186716007212 1106 100-101 -0.8270730816458496 1107 1 0.20900566880177252 1107 2 0.09796775046299899 1107 3 -0.06163473764110705 1107 4 0.04388655234645 1107 5 -0.029050300733523216 1107 6 -0.015166822091025267 1107 7 0.0021784981232855216 1107 8 0.08030463830884571 1107 9 0.09048576256358132 1107 10-11 0.06037766751330054 1107 12-13 0.15691782183896663 1107 14-15 0.15047001343879174 1107 16-17 0.1007456244914664 1107 18-19 0.056787678964425936 1107 20-21 0.11800678417475297 1107 22-23 0.12321127766933415 1107 24-25 0.12086043484203657 1107 26-27 0.24939602394064053 1107 28-29 0.05563422952306496 1107 30-31 0.11566803215111321 1107 32-33 0.16173825741633863 1107 34-35 0.152695082875816 1107 36-37 0.176822100445996 1107 38-39 0.26642519722201286 1107 40-41 0.015673973174990863 1107 42-43 0.18500265505594626 1107 44-45 -0.08216234306585335 1107 46-47 -0.05161934590918804 1107 48-49 -0.306569457300192 1107 50-51 -0.2634480068329381 1107 52-53 -0.33192363566049465 1107 54-55 -0.2860290952627196 1107 56-57 -0.1483605753352748 1107 58-59 -0.2544725897404092 1107 60-61 -0.169227702878878 1107 62-63 -0.17821301364415376 1107 64-65 -0.10121261681766924 1107 66-67 -0.23382102610506905 1107 68-69 -0.12333739583940684 1107 70-71 -0.08719425996259389 1107 72-73 0.01837656901062701 1107 74-75 -0.2574244119782101 1107 76-77 -0.24297949719370493 1107 78-79 -0.23260341987660027 1107 80-81 -0.2640343921939774 1107 82-83 -0.1778745495549714 1107 84-85 -0.10741341458297171 1107 86-87 -0.2717664846468253 1107 88-89 -0.28925696559959135 1107 90-91 -0.0036537696174043788 1107 92-93 0.07361271899843658 1107 94-95 0.06598638244939892 1107 96-97 0.06281727532113379 1107 98-99 -0.4764867360396394 1107 100-101 -0.2587044454736471 1108 1 0.19565148615502181 1108 2 0.056888586992350554 1108 3 0.01895284075532544 1108 4 0.03684638404793361 1108 5 0.08122075914103988 1108 6 0.10091205937808922 1108 7 0.021707104358902996 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0.11671144689153579 2316 54-55 0.1875104541764543 2316 56-57 0.17632346714363223 2316 58-59 0.04519590083641134 2316 60-61 0.17736627186990717 2316 62-63 0.16220304734469693 2316 64-65 0.26950346892726884 2316 66-67 0.36237383605429585 2316 68-69 0.12408939913574812 2316 70-71 0.1455796280764048 2316 72-73 0.1266400245797925 2316 74-75 0.41641808216601817 2316 76-77 0.432560217286067 2316 78-79 0.2672331264585459 2316 80-81 0.21200060275958776 2316 82-83 0.15930179631933328 2316 84-85 0.2474365571696957 2316 86-87 0.06585182089409614 2316 88-89 0.13210515885371166 2316 90-91 0.2528454972215002 2316 92-93 0.3142665019192741 2316 94-95 0.34510573218946305 2316 96-97 0.3386651713709483 2316 98-99 0.3703183002375532 2316 100-101 0.2552771905864546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 6.0 5 27.0 6 39.0 7 108.0 8 281.0 9 479.0 10 917.0 11 1418.0 12 2278.0 13 3322.0 14 4356.0 15 6165.0 16 8403.0 17 12138.0 18 11420.0 19 6827.0 20 5563.0 21 5642.0 22 6125.0 23 6522.0 24 7450.0 25 8768.0 26 10602.0 27 13141.0 28 16500.0 29 20874.0 30 26042.0 31 32853.0 32 42716.0 33 55701.0 34 77118.0 35 111698.0 36 170692.0 37 253393.0 38 180066.0 39 13094.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.72887781899295 19.677363606885663 15.714649676989465 21.879108897131925 2 15.654973703078587 20.53724988978005 40.5027811054059 23.304995301735463 3 19.70688173288028 24.51104648832559 30.231261495161455 25.550810283632668 4 12.92766453945874 17.307872153764624 36.9563261797988 32.80813712697784 5 13.38001564591059 37.553303906666265 34.33903155733834 14.727648890084804 6 31.667906177215865 36.57253808711607 17.130985833953087 14.628569901714977 7 28.058837413327037 33.32614862547929 20.348428642045683 18.26658531914799 8 26.323730466575523 35.8669522736483 19.093836144127614 18.71548111564856 9 26.04602072580394 17.046372538977785 20.321797720785035 36.585809014433245 10-11 24.86196899590739 27.271399370298955 26.72617801746597 21.140453616327694 12-13 25.738296280461736 24.47346622488243 27.924460239418554 21.86377725523728 14-15 22.695868644539548 25.784432044390808 26.57672421855169 24.94297509251796 16-17 21.500594519730484 29.138547590525093 27.30141482335861 22.05944306638581 18-19 21.34040819234829 27.19542553807376 30.36579663417219 21.098369635405763 20-21 22.054143602121567 26.04967245748182 31.01415714024876 20.88202680014785 22-23 21.88123886940052 25.980773093196657 30.836486678462116 21.30150135894071 24-25 21.559283297394884 26.268155150776707 30.975156601674637 21.19740495015377 26-27 21.432484019135174 26.440281843938408 30.793570067753347 21.333664069173075 28-29 21.350389180783136 26.59878529636791 30.902619744678876 21.14820577817007 30-31 21.429430285324937 26.364790181295117 30.9492275696835 21.25655196369644 32-33 21.326246598768208 26.415869891472315 30.77447884890292 21.483404660856554 34-35 21.72994108242671 26.427136819697978 30.73168233497067 21.111239762904642 36-37 21.835306901327538 26.19275799261638 30.633976245930768 21.337958860125315 38-39 21.453300141170605 26.571469287600593 30.683497290147894 21.291733281080912 40-41 21.751284237996522 26.53419401958143 30.591161414298405 21.123360328123646 42-43 21.552630266233397 26.454583190810105 30.490662946810332 21.502123596146173 44-45 21.611081669642974 26.690239633758033 30.333465448828996 21.365213247769994 46-47 21.846083963108605 26.934682989610376 29.728302256502975 21.490930790778044 48-49 21.84252129805701 27.01417495357402 29.539614608708042 21.603689139660922 50-51 22.014553486735753 27.35400866618274 29.18833583462109 21.44310201246042 52-53 22.025597748395686 27.41288170616029 28.97070153328197 21.59081901216205 54-55 21.978847557502554 27.396149380830803 28.98287203480369 21.642131026862952 56-57 21.999501226892775 27.625750942992905 28.90568289609042 21.469064934023898 58-59 21.959911111506962 27.33530467466188 29.12545479646049 21.579329417370662 60-61 21.858998623920627 27.413282505978597 29.206015559939612 21.521703310161165 62-63 21.781822392240517 27.38883371706205 29.424985860673075 21.40435803002436 64-65 21.88113168055364 27.225574591072853 29.30902111324376 21.58427261512975 66-67 21.80275304941862 27.431763830933733 29.090941478773196 21.674541640874455 68-69 21.832100502781103 27.405889975996544 29.089382812813124 21.672626708409226 70-71 21.84639569630062 27.3054673548548 29.041375901242926 21.806761047601658 72-73 21.857128224768537 27.361401196164792 28.89192210232865 21.889548476738025 74-75 21.794692519739392 27.743764222826883 28.524744935448965 21.93679832198476 76-77 21.844614363774824 27.486985139233404 28.764957626552544 21.903442870439232 78-79 21.906114869227927 27.502438198894687 28.66863207022013 21.922814861657265 80-81 21.806182114530774 27.75908368254873 28.592168371550336 21.842565831370155 82-83 21.946039490642146 27.661396556837825 28.540935758456715 21.85162819406331 84-85 21.837187025302505 27.66615497867076 28.554505904897287 21.94215209112945 86-87 21.87943941465413 27.586561627425393 28.636790751321527 21.897208206598947 88-89 21.865455954326634 27.5825981625555 28.512097474515812 22.039848408602055 90-91 21.874635337378063 27.504714447454287 28.623800009442256 21.996850205725394 92-93 21.786237880032136 27.43548338252872 28.66622904445755 22.112049692981596 94-95 21.725443217799075 27.385449185263035 28.689651794024517 22.19945580291337 96-97 21.71248402367391 27.444633958432608 28.580055310374924 22.26282670751856 98-99 21.688979579476335 27.327402620190018 28.49975461882194 22.48386318151171 100-101 21.646200368540356 27.199071032434137 28.843071027089096 22.311657571936415 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 371.0 1 269.5 2 248.5 3 357.5 4 284.0 5 220.0 6 470.5 7 1164.0 8 1967.0 9 2368.5 10 2128.5 11 1749.0 12 1753.5 13 1885.5 14 2014.0 15 2136.0 16 2228.5 17 2256.5 18 2337.0 19 2489.0 20 2676.0 21 2988.5 22 3510.0 23 4248.5 24 5118.0 25 6237.5 26 7611.0 27 9208.0 28 10564.0 29 12044.5 30 13952.0 31 15823.0 32 17886.5 33 19990.5 34 22333.0 35 24750.5 36 27495.0 37 30265.5 38 32161.0 39 33651.5 40 35064.5 41 36859.5 42 38262.5 43 39221.5 44 40992.0 45 42291.0 46 42899.0 47 43840.5 48 44533.0 49 44051.0 50 42491.0 51 40205.0 52 38132.5 53 37107.5 54 36010.0 55 32551.0 56 28078.0 57 24620.0 58 21755.0 59 18970.5 60 15640.5 61 12538.5 62 9816.5 63 7226.0 64 5170.0 65 3805.0 66 2738.0 67 1981.5 68 1466.5 69 1042.0 70 737.0 71 492.0 72 342.5 73 251.5 74 177.0 75 133.0 76 93.5 77 50.5 78 25.0 79 17.0 80 15.0 81 8.5 82 7.0 83 6.0 84 4.5 85 3.0 86 2.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017011725621351052 2 0.0 3 0.0 4 0.0 5 0.03714078316284497 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003117331920142863 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.898664445938785E-4 24-25 1.7813325257959217E-4 26-27 5.343997577387765E-4 28-29 0.001068799515477553 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.679996971734706E-4 42-43 0.011667728043963287 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 4.453331314489804E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.125330103183687E-4 84-85 4.453331314489804E-5 86-87 0.0 88-89 0.0 90-91 0.012647460933151044 92-93 0.0018703991520857175 94-95 0.0 96-97 0.0 98-99 0.001068799515477553 100-101 0.019461057844320444 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1122755.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.12279561935523 #Duplication Level Percentage of deduplicated Percentage of total 1 82.30382606556543 53.5985524355878 2 10.826309261945903 14.100790507552711 3 3.010726838285569 5.882008456661359 4 1.2274855884732636 3.1974917241539327 5 0.6965085618225968 2.267929235935201 6 0.4126283506380662 1.612290704721266 7 0.2656659267402337 1.2110635495091588 8 0.2028932907803242 1.057038264642037 9 0.15475261154083633 0.9070130423641822 >10 0.8251372319396705 9.684303886066914 >50 0.051631508037307546 2.260706504274464 >100 0.021477184972351165 2.353567980664836 >500 2.7359407383343535E-4 0.09220406668136925 >1k 4.1039111075015305E-4 0.6143374336567365 >5k 2.7359407383343535E-4 1.1607022075280773 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6759 0.6020013270927317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6270 0.5584477468370214 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4102 0.36535130104074354 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1674 0.14909753240911866 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 8.906662628979608E-5 0.0 0.0 0.0 0.0 16-17 8.906662628979608E-5 0.0 0.0 4.453331314489804E-5 0.0 18-19 8.906662628979608E-5 0.0 0.0 8.906662628979608E-5 0.0 20-21 8.906662628979608E-5 0.0 0.0 1.7813325257959217E-4 0.0 22-23 8.906662628979608E-5 0.0 0.0 4.007998183040824E-4 0.0 24-25 1.7813325257959217E-4 0.0 0.0 9.351995760428589E-4 0.0 26-27 1.7813325257959217E-4 0.0 0.0 0.0013805327074918393 0.0 28-29 2.6719987886938826E-4 0.0 0.0 0.0019149324652306156 0.0 30-31 3.117331920142863E-4 0.0 0.0 0.0038743982436061294 0.0 32-33 3.5626650515918433E-4 0.0 0.0 0.006724530284879604 0.0 34-35 3.5626650515918433E-4 0.0 0.0 0.008995729255269404 0.0 36-37 3.5626650515918433E-4 0.0 0.0 0.013092794064600025 0.0 38-39 3.5626650515918433E-4 0.0 0.0 0.018614924894567383 0.0 40-41 3.5626650515918433E-4 0.0 0.0 0.028278653847010258 0.0 42-43 3.5626650515918433E-4 0.0 0.0 0.03709624984970007 0.0 44-45 3.5626650515918433E-4 0.0 0.0 0.04609197910496947 0.0 46-47 4.453331314489804E-4 0.0 0.0 0.06270290490801644 0.0 48-49 4.453331314489804E-4 0.0 0.0 0.07543943246745728 0.0 50-51 4.453331314489804E-4 0.0 0.0 0.08986822592640425 0.0 52-53 4.453331314489804E-4 0.0 0.0 0.10429701938535121 0.0 54-55 4.453331314489804E-4 0.0 0.0 0.1205962119963839 0.0 56-57 4.453331314489804E-4 0.0 0.0 0.1597855275638942 0.0 58-59 4.453331314489804E-4 0.0 0.0 0.1962137777164208 0.0 60-61 4.453331314489804E-4 0.0 0.0 0.22636283071551674 0.0 62-63 4.453331314489804E-4 0.0 0.0 0.26230121442344945 0.0 64-65 4.453331314489804E-4 0.0 0.0 0.3026483961327271 0.0 66-67 4.453331314489804E-4 0.0 0.0 0.36499503453558435 0.0 68-69 4.453331314489804E-4 0.0 0.0 0.43535766930452324 0.0 70-71 5.343997577387765E-4 0.0 0.0 0.4821176481066662 0.0 72-73 5.343997577387765E-4 0.0 0.0 0.5335090914758784 0.0 74-75 5.343997577387765E-4 0.0 0.0 0.6162519872990991 0.0 76-77 5.343997577387765E-4 0.0 0.0 0.7201036735530013 0.0 78-79 5.343997577387765E-4 0.0 0.0 0.825068692635526 0.0 80-81 0.001113332828622451 0.0 0.0 0.9104390539342955 0.0 82-83 0.001157866141767349 0.0 0.0 1.0224403364937142 0.0 84-85 0.001157866141767349 0.0 0.0 1.2053386535798105 0.0 86-87 0.001157866141767349 0.0 0.0 1.3809335073101434 0.0 88-89 0.001157866141767349 0.0 0.0 1.598968608467564 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3535 0.0 45.84272 1 GTATCAA 5705 0.0 39.15143 1 TAATCCG 65 1.3250545E-4 36.55303 5 CCGTAAG 90 2.4048839E-5 31.682112 1 ATCAACG 7245 0.0 30.629871 3 TCAACGC 7350 0.0 30.256956 4 TATCAAC 7385 0.0 30.1779 2 CAACGCA 7450 0.0 29.850822 5 AACGCAG 7695 0.0 29.133139 6 CGCAGAG 8730 0.0 25.461588 8 GGACCGA 320 0.0 25.232067 6 ACGCAGA 8905 0.0 25.12123 7 ATCGGAC 120 1.7118426E-4 23.74783 8 GCAGAGT 9165 0.0 23.631227 9 GAGTACT 5515 0.0 22.606728 12-13 GTGTAGG 505 0.0 22.585268 1 AGGACCG 405 0.0 22.292833 5 ACCGAGT 385 0.0 22.205763 8 GTACTTT 5625 0.0 22.122421 14-15 TGGTATC 1755 0.0 21.661053 2 >>END_MODULE