FastQCFastQC Report
Thu 26 May 2016
SRR937634_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937634_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145010
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52690.4601706535314102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46560.4066340031964787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29530.2579016777146051No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15130.1321385839424983No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.10296853302591243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29900.049.2882271
GTATCAA48550.039.3631171
GTACGGG651.3217345E-436.5686841
TATCAAC62950.029.8209112
ATCAACG62900.029.7664553
TCAACGC64000.029.3329454
CAACGCA65600.028.545065
TACCTGG10450.028.1964822
AACGCAG67600.027.9670586
GTACCTG10700.027.5461311
TACATGG51450.026.1409052
GTACATG53750.025.2953241
ACATGGG51500.025.0982343
ACGCAGA77800.024.3004267
CGCAGAG78200.024.2368728
GCAGAGT81700.022.3264479
TGGTATC14100.021.5715032
AGAGTAC73750.020.8354910-11
AGTACTT47950.020.70375412-13
GAGTACT47000.020.66744812-13
Produced by FastQC (version 0.11.5)