FastQCFastQC Report
Thu 26 May 2016
SRR937634_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937634_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145010
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67550.5899511794656814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61530.537375219430398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40570.35432004960655367No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16110.14069746115754447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33350.049.8676571
GTATCAA54200.039.363511
TATCGAG753.076806E-431.6695357
ATCAACG68500.031.003223
TCAACGC69700.030.4694464
TATCAAC70800.029.9289472
CAACGCA71450.029.7896655
AACGCAG73700.028.9370926
TCCCGTA700.00838249327.1488722
TGGTATC15050.025.570452
CGCAGAG84650.025.0849658
ACGCAGA85300.025.0051967
GTACATG46000.023.2418881
GCAGAGT90350.022.871479
GTACCTG9650.022.650471
GAGTACT58400.022.57267812-13
TACATGG46350.022.2433322
GTACTTT60600.021.86792614-15
TACCTGG9600.021.7756582
GTGGTAT18800.021.4836391
Produced by FastQC (version 0.11.5)