##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937634_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1145010 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19877380983572 33.0 31.0 34.0 31.0 34.0 2 32.40281831599724 34.0 31.0 34.0 31.0 34.0 3 32.49350486021956 34.0 31.0 34.0 31.0 34.0 4 36.12230984882228 37.0 35.0 37.0 35.0 37.0 5 36.03742325394538 37.0 35.0 37.0 35.0 37.0 6 36.07719321228635 37.0 35.0 37.0 35.0 37.0 7 36.02457445786499 37.0 35.0 37.0 35.0 37.0 8 36.0009144024943 37.0 35.0 37.0 35.0 37.0 9 37.68270932131597 39.0 37.0 39.0 35.0 39.0 10-11 37.61255840560345 39.0 37.0 39.0 35.0 39.0 12-13 37.51255665889381 39.0 37.0 39.0 35.0 39.0 14-15 38.71967144391752 40.0 38.0 41.0 34.5 41.0 16-17 38.67212120418162 40.0 38.0 41.0 34.5 41.0 18-19 38.6700762438756 40.0 38.0 41.0 34.0 41.0 20-21 38.573055693836736 40.0 38.0 41.0 34.0 41.0 22-23 38.41190993965118 40.0 38.0 41.0 34.0 41.0 24-25 38.49628474860482 40.0 38.0 41.0 34.0 41.0 26-27 38.37557881590554 40.0 38.0 41.0 34.0 41.0 28-29 38.22899712666265 40.0 38.0 41.0 34.0 41.0 30-31 38.260877634256474 40.0 38.0 41.0 34.0 41.0 32-33 38.16462476310251 40.0 37.0 41.0 34.0 41.0 34-35 38.03106479419394 40.0 37.0 41.0 33.5 41.0 36-37 37.86482869145247 40.0 37.0 41.0 33.0 41.0 38-39 37.72585086593131 40.0 37.0 41.0 33.0 41.0 40-41 37.54188435035502 40.0 37.0 41.0 33.0 41.0 42-43 37.319717295045464 40.0 37.0 41.0 32.5 41.0 44-45 36.99242801372914 40.0 36.0 41.0 31.5 41.0 46-47 36.61545139343761 39.5 36.0 41.0 30.5 41.0 48-49 36.259148828394515 39.0 36.0 41.0 30.0 41.0 50-51 35.99200618335211 39.0 35.0 40.5 29.0 41.0 52-53 35.74162802071598 39.0 35.0 40.0 28.5 41.0 54-55 35.5110841826709 39.0 35.0 40.0 28.0 41.0 56-57 35.23838962105134 38.0 35.0 40.0 27.0 41.0 58-59 34.96939983056917 38.0 34.0 40.0 26.5 41.0 60-61 34.60793704858473 38.0 34.0 40.0 26.0 41.0 62-63 34.25124496729286 37.5 34.0 40.0 26.0 41.0 64-65 33.85130653880752 37.0 33.0 39.5 25.0 41.0 66-67 33.40122619016428 36.0 33.0 39.0 23.5 40.0 68-69 32.94813102068977 36.0 32.0 39.0 22.5 40.0 70-71 32.51360075457856 35.0 32.0 38.0 22.0 40.0 72-73 32.002048890402705 35.0 31.0 37.5 20.5 39.5 74-75 31.478668745251134 35.0 31.0 37.0 18.5 39.0 76-77 30.266226059161056 33.5 29.5 35.5 18.0 38.0 78-79 30.865062750543665 34.0 31.0 36.0 18.0 38.5 80-81 30.660034846857233 34.0 31.0 36.0 17.5 37.5 82-83 30.28442895695234 34.0 30.0 35.0 13.5 37.0 84-85 29.87315132618929 34.0 30.0 35.0 7.5 36.5 86-87 29.422156138374337 34.0 29.0 35.0 4.5 36.0 88-89 29.017165788945075 34.0 29.0 35.0 2.0 36.0 90-91 28.447745871215098 33.5 29.0 35.0 2.0 35.0 92-93 27.92418013816473 33.0 27.0 35.0 2.0 35.0 94-95 27.406946664221273 33.0 26.5 35.0 2.0 35.0 96-97 26.798678177483165 33.0 25.5 34.5 2.0 35.0 98-99 25.840162531331604 32.0 24.5 34.0 2.0 35.0 100-101 23.10798115300303 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3270959174426622 1101 2 0.3225573161392461 1101 3 0.24529032821376973 1101 4 0.13845359450434103 1101 5 0.1298142401844231 1101 6 0.1894780927848032 1101 7 0.2015495818106956 1101 8 0.18779922828922935 1101 9 0.30404219503842 1101 10-11 0.32653308493718924 1101 12-13 0.3464415759293189 1101 14-15 0.27468792188897595 1101 16-17 0.41539752309395084 1101 18-19 0.5341256815399404 1101 20-21 0.539267831021391 1101 22-23 0.3884784000461181 1101 24-25 0.4935970391899289 1101 26-27 0.4101542900733719 1101 28-29 0.5603893099272952 1101 30-31 0.6369331638075479 1101 32-33 0.525368949630078 1101 34-35 0.6599659188583971 1101 36-37 0.7113929945257524 1101 38-39 0.567767580544853 1101 40-41 0.6365970019488927 1101 42-43 0.6175592516040851 1101 44-45 0.6928368765572444 1101 46-47 0.8449296328894249 1101 48-49 0.6155835426964629 1101 50-51 0.5160391855920565 1101 52-53 0.31894529189388265 1101 54-55 0.42178213504818984 1101 56-57 0.32578805903204255 1101 58-59 0.4241654938810555 1101 60-61 0.2939718786005443 1101 62-63 0.4059906096541184 1101 64-65 0.4821455220716686 1101 66-67 0.29660314893304474 1101 68-69 0.4015255783331426 1101 70-71 0.37498379194119025 1101 72-73 0.4002912774874581 1101 74-75 0.3359195267889916 1101 76-77 0.3116241473997121 1101 78-79 0.35469827619155225 1101 80-81 0.27707598944829925 1101 82-83 0.19795609141838 1101 84-85 0.2527972594270551 1101 86-87 0.23668931722372477 1101 88-89 0.46180586117007394 1101 90-91 0.39465892853356266 1101 92-93 0.5276495484748942 1101 94-95 0.5047933459138036 1101 96-97 0.47468613427795603 1101 98-99 0.6354910741067883 1101 100-101 1.083600264427556 1102 1 0.33206936079638893 1102 2 0.2881403767927253 1102 3 0.25873166647905066 1102 4 0.11274462689396358 1102 5 0.12127224748749654 1102 6 0.25218556658382596 1102 7 0.2528311771063727 1102 8 0.25179600355360776 1102 9 0.3800042806836075 1102 10-11 0.37149413914119833 1102 12-13 0.23956737618539847 1102 14-15 0.3940520443340816 1102 16-17 0.3908118779097194 1102 18-19 0.26373248905168367 1102 20-21 0.421376772549344 1102 22-23 0.47048538302141907 1102 24-25 0.2717304863374679 1102 26-27 0.3892009183277523 1102 28-29 0.5199823056118404 1102 30-31 0.4599565312851368 1102 32-33 0.4247678683952003 1102 34-35 0.38150495949955143 1102 36-37 0.46074537988165787 1102 38-39 0.5134138910679269 1102 40-41 0.45507817518359417 1102 42-43 0.523708478615994 1102 44-45 0.5348841094495427 1102 46-47 0.6480192141480217 1102 48-49 0.6762948221577432 1102 50-51 0.7191575167913697 1102 52-53 0.8449402769557608 1102 54-55 0.7670096448883754 1102 56-57 0.7929182814439599 1102 58-59 0.6685681471745468 1102 60-61 0.5382945062858511 1102 62-63 0.7907063112712294 1102 64-65 0.894839065487048 1102 66-67 0.803328263932805 1102 68-69 0.7302248361697181 1102 70-71 0.8442139455903614 1102 72-73 0.9329684008336052 1102 74-75 0.7818103600037674 1102 76-77 0.7662703749332636 1102 78-79 0.9634590055508703 1102 80-81 0.8327412097261977 1102 82-83 0.7476949708227529 1102 84-85 0.9626081026005728 1102 86-87 1.0191648945936684 1102 88-89 1.3341161115613112 1102 90-91 1.2150177396494115 1102 92-93 1.2616844396400673 1102 94-95 1.0976215694638576 1102 96-97 1.3640966715758154 1102 98-99 1.4670445786356439 1102 100-101 1.5906751209742431 1103 1 0.28032717075632974 1103 2 0.33127080822103494 1103 3 0.23772509228098926 1103 4 0.22131533120934677 1103 5 0.20634832192527597 1103 6 0.22030235916869856 1103 7 0.26266216566076395 1103 8 0.2771298108023075 1103 9 0.35044932027758335 1103 10-11 0.2909133723902215 1103 12-13 0.2754809307159789 1103 14-15 0.3378283232167405 1103 16-17 0.4840292397440962 1103 18-19 0.35483996313787003 1103 20-21 0.3993066440962352 1103 22-23 0.45576232042601106 1103 24-25 0.43605168523313154 1103 26-27 0.40444542559352925 1103 28-29 0.3706734781115202 1103 30-31 0.49549811735864324 1103 32-33 0.5613092230216665 1103 34-35 0.6468128391691366 1103 36-37 0.5231114023540613 1103 38-39 0.5240308351955107 1103 40-41 0.4503100906282711 1103 42-43 0.5910650778874853 1103 44-45 0.7538226767923675 1103 46-47 0.7904328485829311 1103 48-49 0.8643049011252728 1103 50-51 0.8541386703895455 1103 52-53 0.8246555970966156 1103 54-55 0.8312024457833047 1103 56-57 0.9387926001906166 1103 58-59 0.8832352767352987 1103 60-61 0.8471626476008041 1103 62-63 0.7116313279409283 1103 64-65 0.8944436767937844 1103 66-67 0.9490186649423578 1103 68-69 1.0180361186618612 1103 70-71 0.8864137670455108 1103 72-73 1.0249066044739301 1103 74-75 1.0060343235894322 1103 76-77 1.2147038366645972 1103 78-79 1.1142171323728292 1103 80-81 0.924221341099944 1103 82-83 1.126442208612545 1103 84-85 1.1050878647611881 1103 86-87 1.154777016907918 1103 88-89 1.2913128746091367 1103 90-91 1.3435355968906748 1103 92-93 1.218350114390848 1103 94-95 1.3956103222398468 1103 96-97 1.4649180330354774 1103 98-99 1.753584968899439 1103 100-101 1.9346770607208619 1104 1 0.20402387865254923 1104 2 0.22258040549505154 1104 3 0.04789653925048043 1104 4 0.0062728048483720045 1104 5 0.06656672229016891 1104 6 0.08963105976702224 1104 7 0.168029609517923 1104 8 0.06772303341506358 1104 9 0.24553665359302812 1104 10-11 0.22105946452619918 1104 12-13 0.1169146191064172 1104 14-15 0.1608415238284735 1104 16-17 0.13589798488106908 1104 18-19 0.1552625436964874 1104 20-21 0.020122425803201338 1104 22-23 0.15139833770486177 1104 24-25 0.11868362427420465 1104 26-27 0.17085764957464988 1104 28-29 0.010132440843939605 1104 30-31 0.2339878278574261 1104 32-33 0.1231119650999517 1104 34-35 0.12657726727677954 1104 36-37 0.15362775555092156 1104 38-39 0.06864930531973101 1104 40-41 0.10065553346586142 1104 42-43 0.19704291588485745 1104 44-45 0.23614673571217537 1104 46-47 0.21646045717942286 1104 48-49 0.0931450181062985 1104 50-51 0.18866819736763318 1104 52-53 0.1423073907163257 1104 54-55 0.057925635394539654 1104 56-57 0.03694599022576028 1104 58-59 0.14685511521562233 1104 60-61 0.233496526487869 1104 62-63 0.06112221898221293 1104 64-65 0.08207481091950797 1104 66-67 0.018783361355119155 1104 68-69 0.0208124058556578 1104 70-71 -0.021620629670437097 1104 72-73 0.1900598066954977 1104 74-75 -0.13749885233823278 1104 76-77 0.1060134339386174 1104 78-79 0.23378797833886367 1104 80-81 0.04518670290939397 1104 82-83 0.3135226464864971 1104 84-85 0.21322354665864296 1104 86-87 0.10038419138673405 1104 88-89 0.077057823765319 1104 90-91 0.015706319436354477 1104 92-93 0.21533570640609057 1104 94-95 0.3649685364009869 1104 96-97 0.7207147650791583 1104 98-99 0.5980444682420938 1104 100-101 1.0352713565540839 1105 1 0.2993561374356801 1105 2 0.29908313773548656 1105 3 0.23737905160125905 1105 4 0.13546584385978377 1105 5 0.0786396868693302 1105 6 0.09446325958094093 1105 7 0.14439671255679798 1105 8 0.19192269008317453 1105 9 0.36875873166827233 1105 10-11 0.18477079054734702 1105 12-13 0.28230591262897775 1105 14-15 0.4352675567726081 1105 16-17 0.3440947061925428 1105 18-19 0.45497949144976246 1105 20-21 0.46606829206100997 1105 22-23 0.38997403452444956 1105 24-25 0.32673193972021153 1105 26-27 0.2389120585065143 1105 28-29 0.34267263819425864 1105 30-31 0.44942859000180846 1105 32-33 0.4099978662151216 1105 34-35 0.3206475847932566 1105 36-37 0.4347146892826217 1105 38-39 0.3695138595779639 1105 40-41 0.40685614674573145 1105 42-43 0.3296758259502468 1105 44-45 0.40425225129970244 1105 46-47 0.4263813729058654 1105 48-49 0.491657683383913 1105 50-51 0.5423262545284615 1105 52-53 0.5892357496685108 1105 54-55 0.6631503081153767 1105 56-57 0.545040825832686 1105 58-59 0.6894423568976791 1105 60-61 0.6317379333623734 1105 62-63 0.6770638918734093 1105 64-65 0.6858547377976834 1105 66-67 0.5194523343734261 1105 68-69 0.6148686362811446 1105 70-71 0.6045420467458058 1105 72-73 0.6965211949619352 1105 74-75 0.9485618073307549 1105 76-77 0.6179678048681048 1105 78-79 0.9030194783847243 1105 80-81 0.8629685430110818 1105 82-83 0.9863918920736339 1105 84-85 0.7638247050761713 1105 86-87 0.7918346375871508 1105 88-89 0.787853121952999 1105 90-91 0.9019288168482618 1105 92-93 1.0595370666293853 1105 94-95 1.064317909361634 1105 96-97 1.050214444024526 1105 98-99 0.9210601540540964 1105 100-101 0.6958576416243893 1106 1 0.09851831145586232 1106 2 0.030077289782269645 1106 3 0.12127945007947005 1106 4 0.04429066370315837 1106 5 0.025755678286721206 1106 6 -0.02007969252353803 1106 7 0.012748647550161252 1106 8 0.06538933030483207 1106 9 0.0781701499021068 1106 10-11 -0.10781018226838768 1106 12-13 -0.09848419212932669 1106 14-15 0.047478539865842606 1106 16-17 0.22546479808895015 1106 18-19 0.0972746778570226 1106 20-21 0.19771154888412212 1106 22-23 0.13617106186499228 1106 24-25 0.2419306078533765 1106 26-27 0.29085847130109244 1106 28-29 0.26269451542930966 1106 30-31 0.20077638394710107 1106 32-33 0.06882118350114297 1106 34-35 0.15884920394906032 1106 36-37 0.14100980356713677 1106 38-39 0.21737391739637246 1106 40-41 0.27321569068952556 1106 42-43 -0.017195549130143206 1106 44-45 0.1263722996219414 1106 46-47 0.16087648358423223 1106 48-49 0.17241833801809747 1106 50-51 0.15390916864829052 1106 52-53 0.16336892433178463 1106 54-55 0.33289721735489763 1106 56-57 0.15858307474032785 1106 58-59 -0.0017250099607153402 1106 60-61 0.24218491412362653 1106 62-63 0.313541176211352 1106 64-65 0.13139003888012013 1106 66-67 0.17805928340067112 1106 68-69 0.343506778399032 1106 70-71 0.4296263402132894 1106 72-73 0.566540968616696 1106 74-75 0.46143872384265805 1106 76-77 0.474287260581729 1106 78-79 0.2942132902525252 1106 80-81 0.25502482279928174 1106 82-83 0.0088370938069815 1106 84-85 0.3336335196786244 1106 86-87 0.3212426900925145 1106 88-89 0.09800500045020044 1106 90-91 0.337982981035708 1106 92-93 0.3202968798314565 1106 94-95 0.5967070356545889 1106 96-97 0.8886023802920526 1106 98-99 0.9687122579349037 1106 100-101 0.9633429015367589 1107 1 0.2680045269947158 1107 2 0.322266095128974 1107 3 0.25306725291989807 1107 4 0.15571505802228813 1107 5 0.1798910166325811 1107 6 0.13186266336618502 1107 7 0.27047630001464995 1107 8 0.19289559596899153 1107 9 0.31992035875757807 1107 10-11 0.32989867320362976 1107 12-13 0.36277311781219623 1107 14-15 0.5150015139677606 1107 16-17 0.3967262851399127 1107 18-19 0.3395470253925268 1107 20-21 0.42912341187493297 1107 22-23 0.47681433671879603 1107 24-25 0.38019293032622414 1107 26-27 0.4070865414765308 1107 28-29 0.4167776399071741 1107 30-31 0.373917570246185 1107 32-33 0.3682155026323102 1107 34-35 0.45103800929576465 1107 36-37 0.5284367041519289 1107 38-39 0.6159745857338805 1107 40-41 0.6023719145825908 1107 42-43 0.5733432979876554 1107 44-45 0.6493986154114211 1107 46-47 0.6386855896828152 1107 48-49 0.7248410614934571 1107 50-51 0.7530633039866359 1107 52-53 0.5927757747912636 1107 54-55 0.5324064069956691 1107 56-57 0.6230254306300509 1107 58-59 0.5422390668981052 1107 60-61 0.7741920152096782 1107 62-63 0.7486915521512074 1107 64-65 0.7514819352878064 1107 66-67 0.792929793008021 1107 68-69 0.7389746146563354 1107 70-71 0.7545010269300718 1107 72-73 0.839254001198146 1107 74-75 0.7031889326981258 1107 76-77 0.8067329581590066 1107 78-79 0.8959259051230362 1107 80-81 0.9022679305687049 1107 82-83 0.8236345039991306 1107 84-85 1.0116828095700185 1107 86-87 1.1534795321977782 1107 88-89 0.948360054752623 1107 90-91 1.043079054552834 1107 92-93 1.21973297005702 1107 94-95 1.6021372779269356 1107 96-97 1.5388738924056682 1107 98-99 1.6473881142423394 1107 100-101 1.6355964520797848 1108 1 0.06259517026956729 1108 2 0.04427946187917797 1108 3 0.031053886169694067 1108 4 -0.005834649580052087 1108 5 -0.012673728563740383 1108 6 -0.05433198993372912 1108 7 0.016090335102369124 1108 8 -0.04321551514212274 1108 9 0.07315761857378789 1108 10-11 0.008313460171045506 1108 12-13 -0.006587784443446765 1108 14-15 0.010719976791484953 1108 16-17 -0.031009721543462376 1108 18-19 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0.5641956160732349 2316 76-77 0.4342705521439676 2316 78-79 0.5391589878298042 2316 80-81 0.6093272455227563 2316 82-83 0.5883692575496724 2316 84-85 0.5241932523436184 2316 86-87 0.5738742690398801 2316 88-89 0.6494669887542948 2316 90-91 0.9951258381785664 2316 92-93 0.8097705640419832 2316 94-95 0.901719566982905 2316 96-97 0.8765723051040837 2316 98-99 1.2648693172498575 2316 100-101 1.6554899357890598 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 16.0 5 21.0 6 46.0 7 109.0 8 238.0 9 556.0 10 926.0 11 1512.0 12 2392.0 13 3497.0 14 4648.0 15 6470.0 16 8847.0 17 12703.0 18 11449.0 19 7224.0 20 5987.0 21 5988.0 22 6269.0 23 6835.0 24 7724.0 25 9309.0 26 11191.0 27 14030.0 28 17566.0 29 21762.0 30 27276.0 31 34764.0 32 44622.0 33 58390.0 34 79870.0 35 114079.0 36 171609.0 37 250756.0 38 181855.0 39 14468.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6095228444842 19.777617437001215 15.725149897273196 21.887709821241387 2 15.509384197517925 20.496152872027317 40.60086811468896 23.393594815765802 3 19.636335053842323 24.5347202207841 30.262792464694634 25.566152260678948 4 12.901679117838682 17.280433464977477 37.01246980365168 32.80541761353216 5 13.429159187096268 37.54285739243613 34.29316421860874 14.734819201858866 6 31.703391236757756 36.65068427349979 17.135570868376696 14.510353621365752 7 28.099405245369034 33.29359568912062 20.246198723155256 18.360800342355088 8 26.277237753382067 35.73322503733592 19.171622955258034 18.817914254023982 9 26.045362049239746 17.018803329228565 20.379035990952044 36.55679863057964 10-11 24.866682387053388 27.335394450703486 26.71784525899337 21.08007790324975 12-13 25.700004672593145 24.482117287328233 27.96053536563478 21.857342674443842 14-15 22.669845678203686 25.738552501724875 26.516886315403358 25.07471550466808 16-17 21.455926149116603 29.140051178592323 27.34390966017764 22.06011301211343 18-19 21.316800726631207 27.202513515165805 30.38925424232103 21.091431515881958 20-21 22.054916550947155 26.092610544886067 30.99732753425734 20.855145369909433 22-23 21.860431197259732 26.010351109783596 30.811363862644303 21.317853830312366 24-25 21.613498234511695 26.227959780228797 30.970062244052233 21.188479741207274 26-27 21.479148317013877 26.407910543504887 30.865451502986673 21.24748963649456 28-29 21.375286845604467 26.66455603614855 30.86377917855716 21.096377939689827 30-31 21.46522737792683 26.412607750150652 30.920603313508177 21.201561558414337 32-33 21.38505340564711 26.401297805259343 30.766892865564493 21.44675592352905 34-35 21.69863145300041 26.484790525846936 30.711522170112048 21.105055851040603 36-37 21.766665793311848 26.224749128828567 30.643924507209547 21.364660570650038 38-39 21.454625485640076 26.50563161266348 30.71096790026633 21.32877500143012 40-41 21.772990213567034 26.510923329055323 30.567242807554734 21.148843649822904 42-43 21.572225000382147 26.440580254489156 30.4783845812852 21.5088101638435 44-45 21.666622998925774 26.702343210976327 30.287683077003695 21.343350713094207 46-47 21.833346433655603 26.990986978279665 29.659915633924594 21.515750954140138 48-49 21.870682352119193 27.07137928926385 29.447297403516124 21.61064095510083 50-51 22.034480048209186 27.381900594754633 29.15520388468223 21.428415472353954 52-53 22.086051650203927 27.437183954725285 28.897607881153874 21.579156513916907 54-55 21.983781801032308 27.447533209316948 28.902105658465864 21.66657933118488 56-57 22.024960480694492 27.670937371726012 28.841844176033398 21.4622579715461 58-59 22.006489026296713 27.361638762980235 29.08149273805469 21.55037947266836 60-61 21.887188758176784 27.460327857398624 29.165771477978357 21.48671190644623 62-63 21.826228591890025 27.38032855608248 29.362145308774597 21.431297543252896 64-65 21.88653374206339 27.2701985135501 29.259744456380293 21.58352328800622 66-67 21.779154767207274 27.500589514502057 28.953109579828997 21.767146138461673 68-69 21.838125289844886 27.39982829829285 29.065754068308635 21.69629234355363 70-71 21.835888697866697 27.290865272296866 29.06154927098858 21.81169675884785 72-73 21.88867346136715 27.34845110523052 28.918044383891843 21.844831049510486 74-75 21.81413262766264 27.76792342424957 28.45726238198793 21.96068156609986 76-77 21.855136636361255 27.470895450694755 28.768875381001042 21.90509253194295 78-79 21.951293001807844 27.521550030130744 28.649313106435752 21.877843861625664 80-81 21.931991860333095 27.729146470336502 28.533418922105486 21.805442747224916 82-83 21.906173853046752 27.744347112201854 28.470947344518294 21.878531690233103 84-85 21.903446878490698 27.645464176201546 28.55117916309682 21.899909782210937 86-87 21.8719050488642 27.64901616579768 28.57110418249622 21.907974602841897 88-89 21.87173037790063 27.57561069335639 28.51341909677645 22.039239831966533 90-91 21.902191476910446 27.532252006743008 28.618207862764105 21.94734865358244 92-93 21.800559399638857 27.369009434683218 28.68231035107677 22.14812081460116 94-95 21.713784159090313 27.493995685627198 28.607261071955705 22.18495908332678 96-97 21.740203142330635 27.490545934096644 28.53940140260784 22.22984952096488 98-99 21.67869148545013 27.359905257062707 28.50973761411642 22.451665643370745 100-101 21.61993361285814 27.16491963661775 28.868535988819005 22.3466107617051 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 373.0 1 271.0 2 239.0 3 354.0 4 284.0 5 216.0 6 495.0 7 1215.5 8 1988.5 9 2339.5 10 2070.0 11 1717.5 12 1771.5 13 1878.5 14 2002.5 15 2131.5 16 2163.0 17 2219.5 18 2392.5 19 2568.5 20 2765.5 21 3124.5 22 3617.0 23 4318.5 24 5232.5 25 6403.0 26 7866.5 27 9578.5 28 11040.5 29 12468.0 30 14180.0 31 15977.0 32 18142.0 33 20338.5 34 22880.5 35 25300.0 36 28080.0 37 30874.0 38 32834.0 39 34392.0 40 35519.0 41 37270.0 42 39039.5 43 40379.0 44 41869.0 45 42856.5 46 43518.5 47 44799.5 48 45687.5 49 45036.0 50 43356.5 51 41025.5 52 38877.0 53 37479.5 54 36545.0 55 33341.0 56 28600.0 57 25060.0 58 22232.0 59 19438.5 60 15896.5 61 12698.0 62 10004.0 63 7389.5 64 5277.5 65 3857.0 66 2862.5 67 2097.0 68 1539.5 69 1104.5 70 750.0 71 497.5 72 362.0 73 262.0 74 196.0 75 146.5 76 91.0 77 46.0 78 24.0 79 17.0 80 13.5 81 10.0 82 8.5 83 4.5 84 2.5 85 2.0 86 1.0 87 2.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01973781888367787 2 0.0 3 0.0 4 3.493419271447411E-4 5 0.019650483401891686 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0031004096034095772 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010480257814342233 24-25 8.733548178618528E-5 26-27 5.676806316102043E-4 28-29 0.0010916935223273159 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.366774089309264E-5 40-41 5.676806316102043E-4 42-43 0.014628693199186034 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.733548178618528E-5 70-71 4.366774089309264E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0017467096357237054 84-85 2.620064453585558E-4 86-87 0.0 88-89 0.0 90-91 0.010480257814342232 92-93 0.0028384031580510217 94-95 0.0 96-97 0.0 98-99 0.0014847031903651496 100-101 0.018340451175098908 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1145010.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.13377513817397 #Duplication Level Percentage of deduplicated Percentage of total 1 82.31231652192908 53.613119154415315 2 10.814552395692285 14.08785247922044 3 3.0246325078498346 5.910172009257071 4 1.22388604609965 3.188652740856137 5 0.6837451515245394 2.226745147560802 6 0.40802513554340814 1.5945730457524385 7 0.2765416983538833 1.26085433578374 8 0.19642910097430596 1.0235335114763293 9 0.15136902726958992 0.8873312566555436 >10 0.8346375169539018 9.747281703540294 >50 0.052452386975004116 2.330153721638074 >100 0.02046982388856072 2.315513879487284 >500 2.6933912741512387E-4 0.08999597044576926 >1k 4.0400869112268585E-4 0.5919910375618876 >5k 2.6933912741512387E-4 1.1322300063489177 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6755 0.5899511794656814 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6153 0.537375219430398 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4057 0.35432004960655367 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1611 0.14069746115754447 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.733548178618528E-5 0.0 0.0 0.0 0.0 8 8.733548178618528E-5 0.0 0.0 0.0 0.0 9 8.733548178618528E-5 0.0 0.0 8.733548178618528E-5 0.0 10-11 8.733548178618528E-5 0.0 0.0 8.733548178618528E-5 0.0 12-13 8.733548178618528E-5 0.0 0.0 1.310032226792779E-4 0.0 14-15 1.7467096357237055E-4 0.0 0.0 1.7467096357237055E-4 0.0 16-17 1.7467096357237055E-4 0.0 0.0 1.7467096357237055E-4 0.0 18-19 1.7467096357237055E-4 0.0 0.0 1.7467096357237055E-4 0.0 20-21 1.7467096357237055E-4 0.0 0.0 1.7467096357237055E-4 0.0 22-23 2.620064453585558E-4 0.0 0.0 2.620064453585558E-4 0.0 24-25 3.493419271447411E-4 0.0 0.0 4.3667740893092635E-4 0.0 26-27 3.493419271447411E-4 0.0 0.0 9.60690299648038E-4 0.0 28-29 5.676806316102043E-4 0.0 0.0 0.0018340451175098908 0.0 30-31 6.113483725032969E-4 0.0 0.0 0.0046287805346678194 0.0 32-33 6.113483725032969E-4 0.0 0.0 0.007467183692718841 0.0 34-35 6.550161133963895E-4 0.0 0.0 0.011004270705059344 0.0 36-37 6.986838542894822E-4 0.0 0.0 0.01558938349883407 0.0 38-39 6.986838542894822E-4 0.0 0.0 0.021309857555829207 0.0 40-41 6.986838542894822E-4 0.0 0.0 0.03392983467393298 0.0 42-43 6.986838542894822E-4 0.0 0.0 0.04336206670684099 0.0 44-45 7.860193360756675E-4 0.0 0.0 0.053536650334931574 0.0 46-47 0.0010480257814342233 0.0 0.0 0.07126575313752719 0.0 48-49 0.0011353612632204085 0.0 0.0 0.08331804962402076 0.0 50-51 0.0011353612632204085 0.0 0.0 0.09825241700945843 0.0 52-53 0.0011353612632204085 0.0 0.0 0.11545750692133694 0.0 54-55 0.0011353612632204085 0.0 0.0 0.13296827101946707 0.0 56-57 0.0011353612632204085 0.0 0.0 0.17659234417166664 0.0 58-59 0.0011353612632204085 0.0 0.0 0.21960506895136286 0.0 60-61 0.001179029004113501 0.0 0.0 0.24860044890437638 0.0 62-63 0.0012226967450065939 0.0 0.0 0.28501934480921565 0.0 64-65 0.0012226967450065939 0.0 0.0 0.32833774377516356 0.0 66-67 0.0012226967450065939 0.0 0.0 0.38864289394852447 0.0 68-69 0.0012226967450065939 0.0 0.0 0.4631837276530336 0.0 70-71 0.0012226967450065939 0.0 0.0 0.5131396232347316 0.0 72-73 0.0012226967450065939 0.0 0.0 0.5650168994157256 0.0 74-75 0.0012226967450065939 0.0 0.0 0.645321874918123 0.0 76-77 0.0012226967450065939 0.0 0.0 0.748552414389394 0.0 78-79 0.0012226967450065939 0.0 0.0 0.849468563593331 0.0 80-81 0.0012226967450065939 0.0 0.0 0.937109719565768 0.0 82-83 0.0012226967450065939 0.0 0.0 1.053091239377822 0.0 84-85 0.0012226967450065939 0.0 0.0 1.226059161055362 0.0 86-87 0.0012226967450065939 0.0 0.0 1.3988087440284365 0.0 88-89 0.0012226967450065939 0.0 0.0 1.6167544388258617 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3335 0.0 49.867657 1 GTATCAA 5420 0.0 39.36351 1 TATCGAG 75 3.076806E-4 31.669535 7 ATCAACG 6850 0.0 31.00322 3 TCAACGC 6970 0.0 30.469446 4 TATCAAC 7080 0.0 29.928947 2 CAACGCA 7145 0.0 29.789665 5 AACGCAG 7370 0.0 28.937092 6 TCCCGTA 70 0.008382493 27.148872 2 TGGTATC 1505 0.0 25.57045 2 CGCAGAG 8465 0.0 25.084965 8 ACGCAGA 8530 0.0 25.005196 7 GTACATG 4600 0.0 23.241888 1 GCAGAGT 9035 0.0 22.87147 9 GTACCTG 965 0.0 22.65047 1 GAGTACT 5840 0.0 22.572678 12-13 TACATGG 4635 0.0 22.243332 2 GTACTTT 6060 0.0 21.867926 14-15 TACCTGG 960 0.0 21.775658 2 GTGGTAT 1880 0.0 21.483639 1 >>END_MODULE