FastQCFastQC Report
Thu 26 May 2016
SRR937633_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937633_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087907
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53370.49057502157813127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43820.40279178275348904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28030.25765069992196027No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15000.13787943270886205No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11580.10644292205124152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29900.047.700021
GTATCAA49850.039.2916761
ATCAACG61550.031.6565863
TATCAAC61950.031.3783682
TCAACGC63050.030.9034584
CAACGCA63450.030.7086355
AACGCAG65650.029.816096
ACGCAGA76100.025.1598827
CGCAGAG75950.025.0844618
GTACATG49100.023.625271
TACATGG49300.023.1372992
GCAGAGT80300.023.0145369
GAGTACT43850.022.04260612-13
GTACCTG10250.021.7992971
ACATGGG50300.021.635943
GTACTTT43950.021.56116114-15
GTGTAGG4450.021.366751
AGTACTT44900.020.52218212-13
CGTACAC1404.8353503E-420.3672083
CAGAGTA76300.020.2323810-11
Produced by FastQC (version 0.11.5)