##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937633_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1087907 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.59734609667922 33.0 31.0 34.0 30.0 34.0 2 31.7279886975633 33.0 31.0 34.0 30.0 34.0 3 31.735455328442598 33.0 31.0 34.0 30.0 34.0 4 35.337083960301754 37.0 35.0 37.0 33.0 37.0 5 35.332515555098 37.0 35.0 37.0 33.0 37.0 6 35.37574535323332 37.0 35.0 37.0 33.0 37.0 7 35.317310211258864 37.0 35.0 37.0 33.0 37.0 8 35.297882999190186 37.0 35.0 37.0 33.0 37.0 9 36.89156334135179 39.0 37.0 39.0 33.0 39.0 10-11 36.77349856191752 39.0 37.0 39.0 33.0 39.0 12-13 36.64088612353814 39.0 37.0 39.0 32.0 39.0 14-15 37.76437967583626 40.0 38.0 41.0 32.5 41.0 16-17 37.674853181384066 40.0 37.5 41.0 32.0 41.0 18-19 37.61323256491593 40.0 37.0 41.0 32.0 41.0 20-21 37.53158082446386 40.0 37.0 41.0 32.0 41.0 22-23 37.32666579036627 40.0 37.0 41.0 32.0 41.0 24-25 37.1530493874936 39.5 37.0 41.0 31.0 41.0 26-27 37.0941486726347 39.0 36.5 41.0 31.0 41.0 28-29 37.13836201072334 39.5 37.0 41.0 31.0 41.0 30-31 36.95729460330709 39.0 36.5 41.0 31.0 41.0 32-33 36.70794838161718 39.0 36.0 41.0 30.0 41.0 34-35 36.717294309164295 39.0 36.0 41.0 30.0 41.0 36-37 36.61668690430341 39.0 36.0 41.0 30.0 41.0 38-39 36.48144510514226 39.0 35.5 41.0 30.0 41.0 40-41 36.24568230556472 39.0 35.0 41.0 30.0 41.0 42-43 35.988619891222314 39.0 35.0 41.0 29.0 41.0 44-45 35.74088731849322 39.0 35.0 40.5 27.5 41.0 46-47 35.388841141752 39.0 35.0 40.0 26.5 41.0 48-49 35.02025402906682 39.0 35.0 40.0 24.5 41.0 50-51 34.08597563946183 37.5 33.5 39.5 23.5 40.5 52-53 34.18844579545862 38.0 33.5 39.5 23.5 40.5 54-55 34.52535878526382 38.0 34.0 40.0 23.5 41.0 56-57 34.311966464045184 38.0 34.0 40.0 23.0 41.0 58-59 34.056898245897855 38.0 34.0 40.0 22.0 41.0 60-61 33.7241101491212 38.0 33.0 40.0 20.5 41.0 62-63 33.35432670255821 37.0 33.0 40.0 19.5 41.0 64-65 32.9670376236204 37.0 32.0 39.5 18.0 41.0 66-67 32.54100580288572 36.0 32.0 39.0 15.0 41.0 68-69 32.041253526266495 35.5 31.0 39.0 10.5 40.0 70-71 31.58469106274709 35.0 31.0 38.5 8.0 40.0 72-73 31.117088133452583 35.0 30.5 37.5 7.0 40.0 74-75 30.640158120133428 35.0 30.0 37.0 2.0 39.0 76-77 30.126468530857878 34.5 29.5 36.5 2.0 39.0 78-79 29.57865148399633 34.0 29.0 36.0 2.0 38.5 80-81 29.023089289801426 34.0 29.0 35.5 2.0 37.0 82-83 28.48583426708349 34.0 27.5 35.0 2.0 37.0 84-85 28.006365893408166 34.0 26.5 35.0 2.0 36.5 86-87 27.49764180210257 33.0 26.0 35.0 2.0 36.0 88-89 26.991794335361387 33.0 25.5 35.0 2.0 35.5 90-91 26.474273076650853 32.5 25.0 35.0 2.0 35.0 92-93 25.865398421004738 32.0 23.5 35.0 2.0 35.0 94-95 25.196181750829805 32.0 19.5 34.0 2.0 35.0 96-97 24.43414602534959 31.5 12.5 34.0 2.0 35.0 98-99 23.4653426257943 31.0 2.0 34.0 2.0 35.0 100-101 21.125748800219135 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0693268727323968 1101 2 0.10528191107794882 1101 3 0.12187088788010669 1101 4 0.08168857873134527 1101 5 -0.07766084228790504 1101 6 0.12134207232729466 1101 7 0.04062058080341302 1101 8 -0.12736085334751124 1101 9 0.14303790513795178 1101 10-11 0.0354329919241394 1101 12-13 0.07072660280195464 1101 14-15 0.19766751300927154 1101 16-17 0.29178299831401233 1101 18-19 0.28792322792126157 1101 20-21 0.30649489401665875 1101 22-23 0.26517244482217706 1101 24-25 0.05689970671412681 1101 26-27 0.1820723611943933 1101 28-29 -0.06539969399933199 1101 30-31 0.16943686790063595 1101 32-33 0.07685520933017642 1101 34-35 0.19623681336236842 1101 36-37 0.05683382952130955 1101 38-39 0.14713355556290253 1101 40-41 0.3113622841440602 1101 42-43 0.21863146727883276 1101 44-45 0.36192454139663965 1101 46-47 0.4262408323649822 1101 48-49 0.3085660848088523 1101 50-51 0.2741261580211045 1101 52-53 0.434562628174902 1101 54-55 0.46425578516362265 1101 56-57 0.3296098451122731 1101 58-59 0.3708081243738448 1101 60-61 0.37529354662602543 1101 62-63 0.501289200775723 1101 64-65 0.42120418753014377 1101 66-67 0.5093204962218536 1101 68-69 0.5166482626990145 1101 70-71 0.5806260555147134 1101 72-73 0.5483956181504404 1101 74-75 0.6619047151708983 1101 76-77 0.6795764707645127 1101 78-79 0.7156413137329452 1101 80-81 0.5843007089120817 1101 82-83 0.641616552306921 1101 84-85 0.7979382821737317 1101 86-87 0.9531385061360282 1101 88-89 0.8876262011951752 1101 90-91 0.9709403091305084 1101 92-93 0.8706987130913895 1101 94-95 1.0939445177068627 1101 96-97 1.2362732874345141 1101 98-99 1.088527618781228 1101 100-101 1.4482179128222903 1102 1 0.27447958154539975 1102 2 0.3270273131695447 1102 3 0.3033752291933176 1102 4 0.2476820875665382 1102 5 0.21860257694702767 1102 6 0.2202116664907976 1102 7 0.3208225281528527 1102 8 0.36277756935492533 1102 9 0.3614739227806112 1102 10-11 0.426992397178104 1102 12-13 0.530401628461469 1102 14-15 0.5872122992724158 1102 16-17 0.6034989959838555 1102 18-19 0.6033002148002282 1102 20-21 0.7173329200367959 1102 22-23 0.7242337225275293 1102 24-25 0.6036673008221456 1102 26-27 0.49718789837988453 1102 28-29 0.6149458079563459 1102 30-31 0.6495167591597522 1102 32-33 0.7696608350063414 1102 34-35 0.7623024462509349 1102 36-37 0.9027116624709066 1102 38-39 0.8863302045684236 1102 40-41 1.0547005182143536 1102 42-43 1.0450177748046983 1102 44-45 1.0740684288831162 1102 46-47 1.159438701931407 1102 48-49 1.2440414100605963 1102 50-51 0.8874600923882099 1102 52-53 1.0530132093283342 1102 54-55 1.0198398512958349 1102 56-57 1.236977594564408 1102 58-59 0.9792827006450366 1102 60-61 1.1256673434026325 1102 62-63 1.204699142799079 1102 64-65 1.0578355781373787 1102 66-67 1.381385602050031 1102 68-69 1.3536603850879914 1102 70-71 1.1380583005145724 1102 72-73 1.3086282182225624 1102 74-75 1.3562027539514467 1102 76-77 1.318185724835363 1102 78-79 1.414572629442116 1102 80-81 1.1751159881153903 1102 82-83 1.1355511968185574 1102 84-85 1.2514751347910043 1102 86-87 1.152030985816463 1102 88-89 1.1048777199612978 1102 90-91 1.205912153009674 1102 92-93 1.1593468031909744 1102 94-95 1.2391004166222288 1102 96-97 1.2804548181288489 1102 98-99 1.6113881696980386 1102 100-101 1.896000098181105 1103 1 0.2621186911382445 1103 2 0.1424468298917816 1103 3 0.26758020580677666 1103 4 0.1207099636879434 1103 5 0.046117257983176785 1103 6 0.12306300001274195 1103 7 0.1422744237978577 1103 8 0.05005761821679755 1103 9 0.27866124513412416 1103 10-11 0.09649854325076745 1103 12-13 0.1840223778354897 1103 14-15 0.3276078213250315 1103 16-17 0.2883247140392129 1103 18-19 0.2662226994581829 1103 20-21 0.2573094938359404 1103 22-23 0.3867477028928761 1103 24-25 0.5377963141454742 1103 26-27 0.3578383588395795 1103 28-29 0.2755336046507679 1103 30-31 0.3221051957120338 1103 32-33 0.2642213822170163 1103 34-35 0.19197625094622595 1103 36-37 0.33707533440890813 1103 38-39 0.4515666214701781 1103 40-41 0.3797106316701431 1103 42-43 0.35054461314011576 1103 44-45 0.33193973813276045 1103 46-47 0.5167695692560841 1103 48-49 0.38025908335763603 1103 50-51 0.5537372037208428 1103 52-53 0.4698019425419844 1103 54-55 0.4383802614923127 1103 56-57 0.6372219039277525 1103 58-59 0.4740322967678168 1103 60-61 0.621898924353907 1103 62-63 0.5695998987303241 1103 64-65 0.5617897410472992 1103 66-67 0.47930831145144737 1103 68-69 0.6083297610150638 1103 70-71 0.7969228436639924 1103 72-73 0.7838799970009624 1103 74-75 0.7649139427385983 1103 76-77 0.7460252262750409 1103 78-79 0.800906640531224 1103 80-81 0.6425671857336397 1103 82-83 0.6716787083267484 1103 84-85 0.9008092331334545 1103 86-87 0.8629335555596498 1103 88-89 0.8452752250444675 1103 90-91 0.8776035987447024 1103 92-93 0.7985628186744762 1103 94-95 0.9305871342836269 1103 96-97 1.2044710366746791 1103 98-99 1.3790358195424375 1103 100-101 2.1690812517231954 1104 1 0.1451490840372891 1104 2 0.11636845819986519 1104 3 0.09693980351644882 1104 4 0.09833477540701807 1104 5 -0.04522272989400733 1104 6 -0.16753999323125868 1104 7 -0.004904679165470327 1104 8 0.11907054488934676 1104 9 0.16066666138129904 1104 10-11 0.26007272008266114 1104 12-13 0.34167627391742883 1104 14-15 0.4832566393500528 1104 16-17 0.5522854683595924 1104 18-19 0.4442805247907131 1104 20-21 0.36839068811583786 1104 22-23 0.49203999033379375 1104 24-25 0.45597378078820583 1104 26-27 0.33594118949210383 1104 28-29 0.4308754499729446 1104 30-31 0.5628264254758975 1104 32-33 0.5248600675413755 1104 34-35 0.5482688011349595 1104 36-37 0.6642275902680552 1104 38-39 0.8412040815051967 1104 40-41 0.782420446211809 1104 42-43 0.8596737992202037 1104 44-45 0.9828291785319294 1104 46-47 1.1713908091656577 1104 48-49 1.0050493403562228 1104 50-51 1.1773665575953345 1104 52-53 1.0708115705586962 1104 54-55 1.0303602094110076 1104 56-57 1.1954140428250923 1104 58-59 1.1419236024163766 1104 60-61 1.2613705410854763 1104 62-63 1.383490543607138 1104 64-65 1.2127221055955957 1104 66-67 1.2547031485868203 1104 68-69 1.2572238788457497 1104 70-71 1.282859331532066 1104 72-73 1.2347168878357415 1104 74-75 1.5245647222671614 1104 76-77 1.4381185980059747 1104 78-79 1.3431973332749614 1104 80-81 1.37640884505117 1104 82-83 1.4272438892975607 1104 84-85 1.363266211429444 1104 86-87 1.469258624043178 1104 88-89 1.3876998931627504 1104 90-91 1.451739457578885 1104 92-93 1.5412403490530657 1104 94-95 1.548943971840437 1104 96-97 1.554239116196218 1104 98-99 1.6279419041056116 1104 100-101 2.1648954968174827 1105 1 0.2204615440323039 1105 2 0.11519610156089044 1105 3 0.327249939011228 1105 4 0.28576630371538414 1105 5 0.15785436939921027 1105 6 0.3220051458681823 1105 7 0.4362591879943949 1105 8 0.2925880864439989 1105 9 0.24929278067869376 1105 10-11 0.34412563783577355 1105 12-13 0.4190299146238061 1105 14-15 0.45729803566723604 1105 16-17 0.5697916501826796 1105 18-19 0.4089306032847091 1105 20-21 0.28379437632287363 1105 22-23 0.40667659346411966 1105 24-25 0.2957072380948844 1105 26-27 0.3785371509256166 1105 28-29 0.24693808346326307 1105 30-31 0.29491733027919054 1105 32-33 0.3342695771055162 1105 34-35 0.4061687330843782 1105 36-37 0.3663863435274095 1105 38-39 0.5179937782421433 1105 40-41 0.5714218543873102 1105 42-43 0.6668514516770969 1105 44-45 0.738654915128997 1105 46-47 0.73074405908649 1105 48-49 0.7264832538039627 1105 50-51 0.8176390294486637 1105 52-53 0.8374755421526388 1105 54-55 0.8270243831354023 1105 56-57 0.7782659687914446 1105 58-59 0.752086047925367 1105 60-61 0.6932124885450364 1105 62-63 0.8814945053640457 1105 64-65 0.8479416529581343 1105 66-67 0.9873131917753568 1105 68-69 0.9821081591602692 1105 70-71 1.1762274313036585 1105 72-73 1.3987555217033751 1105 74-75 1.3362717813364178 1105 76-77 1.4014773997766419 1105 78-79 1.3873968629847688 1105 80-81 1.5112810649152024 1105 82-83 1.45437451273526 1105 84-85 1.3217344668414874 1105 86-87 1.2809468176033008 1105 88-89 1.3101848849764686 1105 90-91 1.484130862991936 1105 92-93 1.542823030515681 1105 94-95 1.5074772458821712 1105 96-97 1.6160371339702202 1105 98-99 1.8259859550694344 1105 100-101 1.6848855250483687 1106 1 0.21114474009505813 1106 2 0.10123486853211716 1106 3 0.18633269725716772 1106 4 0.23165556090171435 1106 5 0.4086131742524586 1106 6 0.03322691871577632 1106 7 0.13843197038235644 1106 8 -0.07012994865897326 1106 9 0.005906997304869321 1106 10-11 -0.06525016269363704 1106 12-13 0.047894773614189035 1106 14-15 -0.07928761418798302 1106 16-17 -0.13845661679243193 1106 18-19 0.03532032235225557 1106 20-21 0.2457012082835064 1106 22-23 0.16650573622811038 1106 24-25 -0.024872092914812072 1106 26-27 0.07198620038183634 1106 28-29 -0.03511841353644485 1106 30-31 0.05166855872796816 1106 32-33 0.13710645929087661 1106 34-35 -0.08920546832688814 1106 36-37 -0.11137000054692692 1106 38-39 0.22643766549654742 1106 40-41 0.4078891229227182 1106 42-43 0.09865410807628905 1106 44-45 -0.09263113295322256 1106 46-47 0.21659962611360584 1106 48-49 0.2925225641648481 1106 50-51 0.054751767278673924 1106 52-53 0.10926546770824785 1106 54-55 0.3376717154802762 1106 56-57 0.18686433396482016 1106 58-59 0.17130496409587437 1106 60-61 0.06767822006396784 1106 62-63 0.24159689550230468 1106 64-65 0.4186358720683714 1106 66-67 0.42354253326471536 1106 68-69 0.27246726210155714 1106 70-71 0.3485696736399255 1106 72-73 0.3292633581586131 1106 74-75 0.4936238435001954 1106 76-77 0.4666201171907254 1106 78-79 0.7141704480368958 1106 80-81 0.7290665967772441 1106 82-83 0.6332017225845838 1106 84-85 0.41021305340734315 1106 86-87 0.6312736276730888 1106 88-89 0.6902125978493672 1106 90-91 0.9113981563680191 1106 92-93 0.7004925874978518 1106 94-95 0.975607830181314 1106 96-97 1.1998530490772303 1106 98-99 1.4846440931059348 1106 100-101 1.8374006429251146 1107 1 0.18682180838974105 1107 2 0.07585188644848273 1107 3 0.17195541503809864 1107 4 0.021451319743043484 1107 5 -0.00626454107974439 1107 6 0.06043806894621184 1107 7 0.1205060319306881 1107 8 0.1094827859838503 1107 9 0.22044875084674231 1107 10-11 0.14391157594850768 1107 12-13 0.15683828066603667 1107 14-15 0.44493641138722495 1107 16-17 0.3985043647392459 1107 18-19 0.32671572832203566 1107 20-21 0.36173484912982445 1107 22-23 0.4689754382494655 1107 24-25 0.5272135502222994 1107 26-27 0.6577569583058889 1107 28-29 0.39148526162392017 1107 30-31 0.5170358594041957 1107 32-33 0.43490204903462626 1107 34-35 0.5138483382031538 1107 36-37 0.6156151810814166 1107 38-39 0.6849652882238999 1107 40-41 0.729899964310782 1107 42-43 0.8675515599672323 1107 44-45 0.7969329941124315 1107 46-47 0.8984401415740706 1107 48-49 1.0322985417801576 1107 50-51 0.9410815043638507 1107 52-53 1.173360229352454 1107 54-55 1.1125135055536148 1107 56-57 1.2407047632812365 1107 58-59 1.091922425494566 1107 60-61 1.1635409925942284 1107 62-63 1.0114714442430852 1107 64-65 1.1299647716419585 1107 66-67 1.223283143962206 1107 68-69 1.3303875692848521 1107 70-71 1.4091361186186049 1107 72-73 1.4259787972459996 1107 74-75 1.3486477963315444 1107 76-77 1.4666835287822053 1107 78-79 1.782553667814291 1107 80-81 1.8378916557619895 1107 82-83 1.695346677494559 1107 84-85 1.8303304007449306 1107 86-87 1.8157108278638816 1107 88-89 1.8220899638195966 1107 90-91 1.7745822637481723 1107 92-93 1.8709162247867361 1107 94-95 1.8221521776162142 1107 96-97 1.7728509576911584 1107 98-99 2.0462538382312196 1107 100-101 2.20302430190954 1108 1 0.05587394792473077 1108 2 0.07386571191424451 1108 3 0.14062730519532352 1108 4 0.11658538086226145 1108 5 0.1652968517940323 1108 6 0.2799907531504999 1108 7 0.2559373171615036 1108 8 0.10082771527498124 1108 9 0.20631226117432533 1108 10-11 0.18621460231684495 1108 12-13 0.2630812552624775 1108 14-15 0.3825315775321201 1108 16-17 0.4613742246350512 1108 18-19 0.4654908980452106 1108 20-21 0.5471862527761999 1108 22-23 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96-97 0.38959402067842674 2316 98-99 0.6832599684946778 2316 100-101 0.7860710201669221 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5374.0 3 1176.0 4 1132.0 5 1247.0 6 1348.0 7 1601.0 8 2042.0 9 2409.0 10 2933.0 11 3743.0 12 4645.0 13 5812.0 14 6574.0 15 7873.0 16 9496.0 17 12249.0 18 11174.0 19 8002.0 20 8156.0 21 8578.0 22 9385.0 23 10180.0 24 11436.0 25 12795.0 26 15083.0 27 17124.0 28 20161.0 29 24040.0 30 29079.0 31 35626.0 32 44613.0 33 57782.0 34 76705.0 35 106158.0 36 150134.0 37 199024.0 38 146151.0 39 16863.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.67715534446886 19.072105549906567 14.89310509173944 22.35763401388514 2 15.001391799993732 19.950485102209736 41.52716406464588 23.52095903315065 3 19.412507960440642 24.677965039495767 29.71467753188093 26.194849468182664 4 12.422825570123221 16.594760063545657 37.40338222122292 33.5790321451082 5 13.351664350717085 37.5407748417844 34.27113933661867 14.836421470879852 6 31.62936619128019 36.24543243036615 17.613801221948652 14.511400156405005 7 28.206807307424857 33.08843236671594 20.480039051526834 18.22472127433237 8 26.09162220715675 35.77676413370276 19.336847108412428 18.794766550728056 9 26.074184476474105 17.329730994453712 20.437531261773543 36.15855326729864 10-11 24.777752649903253 27.270432685110933 26.83784429857995 21.113970366405862 12-13 25.63754655229747 24.481465207943575 27.863398080520852 22.017590159238104 14-15 22.76139320053221 25.773666396937895 26.346636626526664 25.118303776003227 16-17 21.67270523335794 29.209960203949574 26.94617700468977 22.171157558002708 18-19 21.533995579600578 27.50208746201003 29.742085030283743 21.221831928105644 20-21 22.389903047818507 26.403421278273115 30.124388350265658 21.08228732364272 22-23 22.023509953443313 26.397564004510627 29.93736419431052 21.641561847735545 24-25 21.705963204534623 26.653620262140638 30.07332846779188 21.567088065532857 26-27 21.547191586110156 26.8275171923736 29.93856198856344 21.686729232952807 28-29 21.462407211492625 27.0255494539658 30.00371683690271 21.508326497638862 30-31 21.66698828406907 26.72580742471044 30.060741936806096 21.546462354414395 32-33 21.429127404819333 26.82159695985608 29.98858241913622 21.760693216188372 34-35 21.81690988630612 26.894103549070923 29.906321254404507 21.38266531021845 36-37 21.81044907116195 26.701181320275296 29.841667902293107 21.646701706269646 38-39 21.533799963160803 27.073816540799413 29.785549825013813 21.60683367102597 40-41 21.857915193744265 26.965105265314826 29.706225943418595 21.470753597522314 42-43 21.60160150018605 26.90419919464472 29.59791182354393 21.8962874816253 44-45 21.776350447785386 27.13688123280051 29.47472250568251 21.612045813731594 46-47 21.83946103262171 27.35637053546759 28.94969515003039 21.854473281880306 48-49 21.888640506368755 27.417101182564835 28.81634829799639 21.877910013070018 50-51 22.07011027962314 27.679384011112706 28.61922947962609 21.631276229638065 52-53 22.00036666279736 27.74154142091968 28.434561745830823 21.823530170452138 54-55 21.99279560404743 27.691885429657205 28.450502244581045 21.86481672171432 56-57 21.99950183726285 28.02404809250173 28.29113146017391 21.685318610061504 58-59 22.008944172360177 27.673709446539625 28.553715450452295 21.763630930647903 60-61 21.986762956168754 27.68401461655487 28.658559046387673 21.67066338088871 62-63 21.86088340364866 27.67900477333783 28.81588435684269 21.644227466170822 64-65 21.907917001443472 27.682638493999338 28.70812604030214 21.701318464255046 66-67 21.741022299783126 27.785262711981474 28.505412635776352 21.968302352459055 68-69 21.729983063849932 27.739461413850808 28.609799022510618 21.920756499788645 70-71 21.81651915176553 27.688398062767583 28.593977782054907 21.901105003411985 72-73 21.819445979297896 27.716552473569823 28.44867757026559 22.01532397686669 74-75 21.735967248125107 28.028344314629443 28.231454932327928 22.004233504917526 76-77 21.836986946097966 27.85731982842864 28.267919526880096 22.037773698593295 78-79 21.963019473058118 27.795357653893255 28.217167221081358 22.02445565196727 80-81 21.817844395150168 28.086624278910026 28.14142412362845 21.954107202311356 82-83 21.925536573875586 27.98933828964098 28.081532243246514 22.003592893236913 84-85 21.921987190497262 27.933400532033197 28.039171715394932 22.10544056207461 86-87 21.919593972955475 27.943415184018995 28.16623827289313 21.970752570132404 88-89 21.972397850403162 27.83640407631345 28.07931588666264 22.11188218662074 90-91 21.957044309568325 27.891726608766128 28.082558921318512 22.068670160347033 92-93 21.961993869028184 27.761136159241335 28.199707404274367 22.077162567456117 94-95 21.833498495124353 27.85074470162202 28.12330770562643 22.192449097627197 96-97 21.82438844260231 27.83799973571199 28.022817172090637 22.314794649595076 98-99 21.833002874211573 27.76275644349241 27.99745455918472 22.406786123111296 100-101 22.13514771637388 27.303091262625866 28.442268298039764 22.119492722960487 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1566.0 1 921.5 2 324.0 3 359.5 4 299.5 5 277.0 6 453.0 7 965.0 8 1628.0 9 1986.5 10 1799.5 11 1479.0 12 1473.0 13 1606.5 14 1702.0 15 1757.0 16 1808.0 17 1912.0 18 2003.0 19 2114.5 20 2337.5 21 2679.0 22 3196.5 23 3855.5 24 4630.0 25 5813.0 26 7378.5 27 9044.0 28 10370.5 29 11701.0 30 13416.5 31 15196.0 32 17150.0 33 19403.5 34 21870.0 35 24021.0 36 26576.0 37 29353.5 38 31168.0 39 32518.0 40 33891.5 41 35459.5 42 36869.5 43 38255.5 44 39702.0 45 40569.5 46 41380.0 47 42464.0 48 43149.0 49 42807.0 50 41184.0 51 38834.0 52 36710.0 53 35465.5 54 34529.5 55 31613.5 56 27676.5 57 24314.0 58 21706.0 59 18993.5 60 15599.0 61 12618.5 62 9681.5 63 7131.0 64 5363.5 65 4066.5 66 2966.0 67 2134.0 68 1550.0 69 1121.0 70 814.5 71 562.5 72 407.0 73 297.5 74 212.0 75 149.5 76 108.0 77 68.5 78 39.5 79 28.5 80 20.5 81 16.0 82 8.0 83 2.0 84 4.0 85 5.5 86 4.5 87 3.0 88 2.5 89 4.5 90 2.5 91 1.0 92 1.5 93 2.5 94 2.5 95 1.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2895468086886103 2 0.27401239260341187 3 0.26233860063406156 4 0.24845873774136945 5 0.24625266681802763 6 0.20810602376857582 7 0.19873022234437315 8 0.2091171396084408 9 0.21288584410248304 10-11 0.21587323181117504 12-13 0.2114151301535885 14-15 0.20668126963058422 16-17 0.20649743038697244 18-19 0.2076004658486433 20-21 0.1943180804976896 22-23 0.18627511358967266 24-25 0.18747006867314947 26-27 0.20024689610417065 28-29 0.21201260769532687 30-31 0.20668126963058422 32-33 0.21146108996449145 34-35 0.20484287719446606 36-37 0.191928170330736 38-39 0.19367464314504823 40-41 0.18894078262204397 42-43 0.19992517742785 44-45 0.19477767860671913 46-47 0.19551303558116642 48-49 0.20369388192189222 50-51 0.2116908890190062 52-53 0.2243757968282215 54-55 0.20047669515868544 56-57 0.17616395519102276 58-59 0.17910538308881183 60-61 0.1852180379389047 62-63 0.19156049184351234 64-65 0.1824144894738245 66-67 0.18586147529154606 68-69 0.18972209940739418 70-71 0.1864129930223815 72-73 0.1863210734005756 74-75 0.1880215864039849 76-77 0.20020093629326774 78-79 0.20475095757266018 80-81 0.19647819161012844 82-83 0.19785698593721707 84-85 0.1905034161927444 86-87 0.19008977789461784 88-89 0.19505343747213685 90-91 0.1964322317992255 92-93 0.1938125225777571 94-95 0.19330696465782463 96-97 0.1811276147685418 98-99 0.18788370697127604 100-101 0.18305792682646588 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1087907.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.71186789230723 #Duplication Level Percentage of deduplicated Percentage of total 1 83.72064860678238 56.68881498320724 2 10.12502413019258 13.71168592620046 3 2.681468616500541 5.447017461535573 4 1.101288415933005 2.982811829239357 5 0.6137170091772549 2.0777962524336098 6 0.38195636758688695 1.5517787461601296 7 0.24511630457800548 1.1618097983685514 8 0.18189081793457668 0.985293362784781 9 0.13008830578539968 0.7927669958107545 >10 0.7558366435390702 9.171343765014393 >50 0.04448378329001792 2.0231911689166973 >100 0.017665662165472936 1.940838512267477 >500 1.3588942245131857E-4 0.06808981699529351 >1k 5.435576898052743E-4 0.9056865793036137 >5k 1.3588942245131857E-4 0.491074801762002 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5337 0.49057502157813127 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4382 0.40279178275348904 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2803 0.25765069992196027 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1500 0.13787943270886205 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1158 0.10644292205124152 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.191962180590804E-5 0.0 0.0 0.0 0.0 8 9.191962180590804E-5 0.0 0.0 0.0 0.0 9 9.191962180590804E-5 0.0 0.0 0.0 0.0 10-11 1.838392436118161E-4 0.0 0.0 0.0 0.0 12-13 1.838392436118161E-4 0.0 0.0 0.0 0.0 14-15 1.838392436118161E-4 0.0 0.0 0.0 0.0 16-17 1.838392436118161E-4 0.0 0.0 0.0 0.0 18-19 1.838392436118161E-4 0.0 0.0 4.595981090295402E-5 0.0 20-21 1.838392436118161E-4 0.0 0.0 1.838392436118161E-4 0.0 22-23 1.838392436118161E-4 0.0 0.0 3.2171867632067814E-4 0.0 24-25 2.757588654177241E-4 0.0 0.0 3.676784872236322E-4 0.0 26-27 2.757588654177241E-4 0.0 0.0 5.974775417384023E-4 0.0 28-29 2.757588654177241E-4 0.0 0.0 0.001976271868827023 0.0 30-31 2.757588654177241E-4 0.0 0.0 0.004412141846683586 0.0 32-33 2.757588654177241E-4 0.0 0.0 0.007123770689957874 0.0 34-35 2.757588654177241E-4 0.0 0.0 0.009605600478717391 0.0 36-37 3.676784872236322E-4 0.0 0.0 0.014661179678042334 0.0 38-39 3.676784872236322E-4 0.0 0.0 0.02127939244806771 0.0 40-41 3.676784872236322E-4 0.0 0.0 0.03074711349407624 0.0 42-43 4.1363829812658616E-4 0.0 0.0 0.03874412059119024 0.0 44-45 4.595981090295402E-4 0.0 0.0 0.04922295747706376 0.0 46-47 4.595981090295402E-4 0.0 0.0 0.0657684894021272 0.0 48-49 4.595981090295402E-4 0.0 0.0 0.07730440193876867 0.0 50-51 4.595981090295402E-4 0.0 0.0 0.09214942086042281 0.0 52-53 4.595981090295402E-4 0.0 0.0 0.10832727429826264 0.0 54-55 4.595981090295402E-4 0.0 0.0 0.12358593151804337 0.0 56-57 4.595981090295402E-4 0.0 0.0 0.16720179206494673 0.0 58-59 4.595981090295402E-4 0.0 0.0 0.20957673771747032 0.0 60-61 4.595981090295402E-4 0.0 0.0 0.2413809268623145 9.191962180590804E-5 62-63 4.595981090295402E-4 0.0 0.0 0.2839856715693529 9.191962180590804E-5 64-65 4.595981090295402E-4 0.0 0.0 0.32488990327298195 9.191962180590804E-5 66-67 4.595981090295402E-4 0.0 0.0 0.3855568536648813 9.191962180590804E-5 68-69 4.595981090295402E-4 0.0 0.0 0.46024154638218157 9.191962180590804E-5 70-71 4.595981090295402E-4 0.0 0.0 0.5068907544486798 9.191962180590804E-5 72-73 4.595981090295402E-4 0.0 0.0 0.5579521043618618 9.191962180590804E-5 74-75 4.595981090295402E-4 0.0 0.0 0.6444025086703183 9.191962180590804E-5 76-77 4.595981090295402E-4 0.0 0.0 0.7494666363944712 9.191962180590804E-5 78-79 4.595981090295402E-4 0.0 0.0 0.8538873267659828 9.191962180590804E-5 80-81 7.353569744472644E-4 0.0 0.0 0.9398781329654098 9.191962180590804E-5 82-83 7.353569744472644E-4 0.0 0.0 1.0508710762960436 9.191962180590804E-5 84-85 7.353569744472644E-4 0.0 0.0 1.2309416154138175 9.191962180590804E-5 86-87 7.353569744472644E-4 0.0 0.0 1.4044399015724691 9.191962180590804E-5 88-89 7.353569744472644E-4 0.0 0.0 1.6195777764091968 9.191962180590804E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2990 0.0 47.70002 1 GTATCAA 4985 0.0 39.291676 1 ATCAACG 6155 0.0 31.656586 3 TATCAAC 6195 0.0 31.378368 2 TCAACGC 6305 0.0 30.903458 4 CAACGCA 6345 0.0 30.708635 5 AACGCAG 6565 0.0 29.81609 6 ACGCAGA 7610 0.0 25.159882 7 CGCAGAG 7595 0.0 25.084461 8 GTACATG 4910 0.0 23.62527 1 TACATGG 4930 0.0 23.137299 2 GCAGAGT 8030 0.0 23.014536 9 GAGTACT 4385 0.0 22.042606 12-13 GTACCTG 1025 0.0 21.799297 1 ACATGGG 5030 0.0 21.63594 3 GTACTTT 4395 0.0 21.561161 14-15 GTGTAGG 445 0.0 21.36675 1 AGTACTT 4490 0.0 20.522182 12-13 CGTACAC 140 4.8353503E-4 20.367208 3 CAGAGTA 7630 0.0 20.23238 10-11 >>END_MODULE