FastQCFastQC Report
Thu 26 May 2016
SRR937633_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937633_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087907
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65840.6051987899700986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60320.5544591587332374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38510.3539824635745519No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15950.14661179678042333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34250.046.34851
GTATCAA54900.038.35141
ATCAACG68550.030.351273
TATCAAC68850.030.219022
TCAACGC70050.029.701354
CAACGCA70350.029.5746925
AACGCAG72850.028.6223496
CTATACG700.00836726327.1588751
ACTGCGC1151.281759E-424.7824178
ACGCAGA85850.024.2339427
CGCAGAG85800.024.1373428
GAGTACT55600.023.2372312-13
TACATGG46100.022.772022
GTATAGG3550.022.7599011
GTACATG47250.022.5318071
GCAGAGT91450.022.3863779
ACATGGG45000.022.0619543
GTACTTT58300.022.03783814-15
TACCTGG8450.021.9239162
TGTACCG1604.987502E-520.7820455
Produced by FastQC (version 0.11.5)