##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937633_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1087907 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25791910521763 33.0 31.0 34.0 31.0 34.0 2 32.465253923359256 34.0 31.0 34.0 31.0 34.0 3 32.52291418292189 34.0 31.0 34.0 31.0 34.0 4 36.126104529155526 37.0 35.0 37.0 35.0 37.0 5 36.04827434698003 37.0 35.0 37.0 35.0 37.0 6 36.0969632514544 37.0 35.0 37.0 35.0 37.0 7 36.053905343011856 37.0 35.0 37.0 35.0 37.0 8 36.02250192341808 37.0 35.0 37.0 35.0 37.0 9 37.72428066001965 39.0 37.0 39.0 35.0 39.0 10-11 37.6410065382427 39.0 37.0 39.0 35.0 39.0 12-13 37.5318322246295 39.0 37.0 39.0 35.0 39.0 14-15 38.75930617231069 40.0 38.0 41.0 35.0 41.0 16-17 38.713150113015175 40.0 38.0 41.0 34.5 41.0 18-19 38.69972019667122 40.0 38.0 41.0 34.5 41.0 20-21 38.60245590845541 40.0 38.0 41.0 34.0 41.0 22-23 38.55295765171104 40.0 38.0 41.0 34.0 41.0 24-25 38.57512406850953 40.0 38.0 41.0 34.0 41.0 26-27 38.3924646132436 40.0 38.0 41.0 34.0 41.0 28-29 38.35921498804585 40.0 38.0 41.0 34.0 41.0 30-31 38.3540463477117 40.0 38.0 41.0 34.0 41.0 32-33 38.208674546629446 40.0 38.0 41.0 34.0 41.0 34-35 38.05705175166627 40.0 37.0 41.0 33.5 41.0 36-37 37.884417969550704 40.0 37.0 41.0 33.0 41.0 38-39 37.74256531118928 40.0 37.0 41.0 33.0 41.0 40-41 37.564945808786966 40.0 37.0 41.0 33.0 41.0 42-43 37.32851107677403 40.0 37.0 41.0 32.5 41.0 44-45 37.001645820828436 40.0 36.0 41.0 31.5 41.0 46-47 36.62615738293806 39.5 36.0 41.0 30.5 41.0 48-49 36.23717882135146 39.0 36.0 41.0 30.0 41.0 50-51 35.97798662937181 39.0 35.5 41.0 29.0 41.0 52-53 35.70582274036292 39.0 35.0 40.0 28.0 41.0 54-55 35.48023222573253 39.0 35.0 40.0 28.0 41.0 56-57 35.1922724093144 38.0 35.0 40.0 27.0 41.0 58-59 34.93549632459393 38.0 34.0 40.0 26.5 41.0 60-61 34.5841110499335 38.0 34.0 40.0 26.0 41.0 62-63 34.1699428351872 37.5 34.0 40.0 25.5 41.0 64-65 33.771049823192605 37.0 33.0 39.5 24.5 41.0 66-67 33.33725539039642 36.0 33.0 39.0 23.0 40.5 68-69 32.852013085677356 36.0 32.0 39.0 22.0 40.0 70-71 32.41732565375533 35.0 32.0 38.0 21.0 40.0 72-73 31.92637605971834 35.0 31.0 37.5 19.5 39.5 74-75 31.440932910625634 35.0 31.0 37.0 18.0 39.0 76-77 30.19456350588791 33.5 29.5 35.5 18.0 38.0 78-79 30.792286013418426 34.0 31.0 36.0 17.5 38.0 80-81 30.61300736184251 34.0 31.0 36.0 15.0 37.5 82-83 30.20582136156859 34.0 30.0 35.0 9.5 37.0 84-85 29.780298775538718 34.0 30.0 35.0 7.0 36.5 86-87 29.42493246205788 34.0 29.5 35.0 2.0 36.0 88-89 29.005670061871097 34.0 29.0 35.0 2.0 36.0 90-91 28.484076304316453 34.0 29.0 35.0 2.0 35.0 92-93 27.997548044088326 33.0 27.5 35.0 2.0 35.0 94-95 27.4851526830878 33.0 27.0 35.0 2.0 35.0 96-97 26.9385338084965 33.0 26.0 35.0 2.0 35.0 98-99 26.08156211882082 32.5 24.5 34.0 2.0 35.0 100-101 23.58204883321828 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19684376993874508 1101 2 0.2924267256867452 1101 3 0.2701003579178263 1101 4 0.1411228310646706 1101 5 0.1283365985629743 1101 6 0.1692968601609408 1101 7 0.09426130283134881 1101 8 0.23817230673407153 1101 9 0.18639236986055607 1101 10-11 0.20319897027693656 1101 12-13 0.19872404439841063 1101 14-15 0.18305260170289728 1101 16-17 0.2469116811558436 1101 18-19 0.19483977053157275 1101 20-21 0.25587211503357565 1101 22-23 0.17292535888123695 1101 24-25 0.13266210171685344 1101 26-27 0.24433124741850065 1101 28-29 0.3178042614868275 1101 30-31 0.36387950840762784 1101 32-33 0.320448700623011 1101 34-35 0.3621692381340367 1101 36-37 0.2946801354759856 1101 38-39 0.16764092461925628 1101 40-41 0.10407689696496902 1101 42-43 0.4024554313725446 1101 44-45 0.3131376649551001 1101 46-47 0.3021359337647951 1101 48-49 0.2787336560617675 1101 50-51 0.3143269922843288 1101 52-53 0.2423165336477311 1101 54-55 0.3120349834890348 1101 56-57 0.44062441880959824 1101 58-59 0.38265312602661083 1101 60-61 0.3187776817165897 1101 62-63 0.2633605092894342 1101 64-65 0.32698001879299454 1101 66-67 0.14255751895624513 1101 68-69 0.3751622317963097 1101 70-71 0.36963519805958356 1101 72-73 0.16926650569372015 1101 74-75 0.0410800908522333 1101 76-77 0.29694380835697487 1101 78-79 0.2351589547091102 1101 80-81 0.27609824543630523 1101 82-83 0.20174511114359106 1101 84-85 0.30104716302086487 1101 86-87 0.27241957993503263 1101 88-89 0.43776687603021003 1101 90-91 0.4552602232241121 1101 92-93 0.449247610459107 1101 94-95 0.7133272009549358 1101 96-97 0.7692478887720924 1101 98-99 0.7406788346770945 1101 100-101 1.301271962910878 1102 1 0.2311762063447489 1102 2 0.21085442132324062 1102 3 0.213989231395594 1102 4 0.1250197491483931 1102 5 0.130371329068673 1102 6 0.15582353219067357 1102 7 0.17575870913942993 1102 8 0.11892766749303263 1102 9 0.24547583958232622 1102 10-11 0.24251767537277402 1102 12-13 0.21952905979411952 1102 14-15 0.2985148891449825 1102 16-17 0.35639946167680847 1102 18-19 0.47464989009105807 1102 20-21 0.39046808163459445 1102 22-23 0.23294921529889479 1102 24-25 0.3026335294957079 1102 26-27 0.30855494294404195 1102 28-29 0.46592556859660306 1102 30-31 0.3249144885735049 1102 32-33 0.4107854644722124 1102 34-35 0.20513922343182855 1102 36-37 0.37037779699225126 1102 38-39 0.34767435134398994 1102 40-41 0.5636444300449384 1102 42-43 0.5474240851493448 1102 44-45 0.544506773668239 1102 46-47 0.5370835051259988 1102 48-49 0.5828689996219154 1102 50-51 0.5592963395616479 1102 52-53 0.4119460656735896 1102 54-55 0.4869642464366848 1102 56-57 0.6230157472791902 1102 58-59 0.7590546905637652 1102 60-61 0.7500621833671133 1102 62-63 0.8601412799072037 1102 64-65 0.9408035645224189 1102 66-67 0.8883091348124381 1102 68-69 0.8784112817006076 1102 70-71 0.7600503274667787 1102 72-73 0.885441018162858 1102 74-75 0.8814536102286397 1102 76-77 0.8632493924231106 1102 78-79 0.7721412565760133 1102 80-81 0.8217014521826762 1102 82-83 1.010198188898869 1102 84-85 1.0061027817620207 1102 86-87 0.9578200071313461 1102 88-89 1.0150311552057758 1102 90-91 1.0248814332438059 1102 92-93 1.3449049102455106 1102 94-95 1.4529454982724772 1102 96-97 1.2872775151140132 1102 98-99 1.4108326530909174 1102 100-101 1.7504848398284096 1103 1 0.17267548319960468 1103 2 0.1693495919828507 1103 3 0.14168793766646814 1103 4 0.0791311002796462 1103 5 0.13884151354339735 1103 6 0.10915885736802267 1103 7 0.0429921700036644 1103 8 0.004393111202730893 1103 9 0.1361500435277634 1103 10-11 0.17551900532526332 1103 12-13 0.21848664673679252 1103 14-15 0.29489784142798214 1103 16-17 0.24275859115283538 1103 18-19 0.24168610551692638 1103 20-21 0.3141385918551336 1103 22-23 0.2829771099285736 1103 24-25 0.15506565036010045 1103 26-27 0.3699931953388784 1103 28-29 0.2911723236976087 1103 30-31 0.2554733859027678 1103 32-33 0.18114391707381827 1103 34-35 0.15769852265527362 1103 36-37 0.09736010042765741 1103 38-39 0.2487442624248999 1103 40-41 0.26590296963548354 1103 42-43 0.388450475319857 1103 44-45 0.5842453838017931 1103 46-47 0.3633164874188495 1103 48-49 0.41643456786592736 1103 50-51 0.44103765190482846 1103 52-53 0.44394094152265495 1103 54-55 0.45843636899326157 1103 56-57 0.5044979436309376 1103 58-59 0.42961514787705113 1103 60-61 0.4647450703423104 1103 62-63 0.5359475140264394 1103 64-65 0.7181323694953363 1103 66-67 0.7275812039593959 1103 68-69 0.7372723875970877 1103 70-71 0.49751162532061244 1103 72-73 0.35840768474139395 1103 74-75 0.5924169929085821 1103 76-77 0.6561344110239347 1103 78-79 0.6383468466069218 1103 80-81 0.6886910002896762 1103 82-83 0.6999742122087333 1103 84-85 0.5355752427558151 1103 86-87 0.9605579113060188 1103 88-89 0.9274426789928896 1103 90-91 1.1470533011792625 1103 92-93 1.2887084685955266 1103 94-95 1.2918368255544834 1103 96-97 1.315633529910066 1103 98-99 1.0944718578692267 1103 100-101 1.3589189331471836 1104 1 0.11420368696704486 1104 2 0.14404385497520877 1104 3 0.10981350511070787 1104 4 0.08495317577931871 1104 5 0.08194750497417402 1104 6 0.11901710361736662 1104 7 0.13775048102944254 1104 8 0.10021412010233632 1104 9 0.14549059852400603 1104 10-11 0.04853181233048787 1104 12-13 0.13318595656850363 1104 14-15 0.12009509740418878 1104 16-17 0.2127929825014121 1104 18-19 0.17633148700854662 1104 20-21 0.1486243735559114 1104 22-23 0.16243244149811886 1104 24-25 0.16083699731990464 1104 26-27 0.12604745146337137 1104 28-29 0.04921409799983678 1104 30-31 0.11550619035855192 1104 32-33 0.13452113709779923 1104 34-35 0.10073947749646806 1104 36-37 0.12491090050778553 1104 38-39 0.022618544095770687 1104 40-41 0.14536350413219168 1104 42-43 0.23129150768467355 1104 44-45 0.1646722319465539 1104 46-47 0.3381869995689897 1104 48-49 0.44957005989374466 1104 50-51 0.2527300600536506 1104 52-53 0.3949469274903592 1104 54-55 0.5365641736961848 1104 56-57 0.4868190474839693 1104 58-59 0.6589679817814087 1104 60-61 0.6008848625894316 1104 62-63 0.5290705450436874 1104 64-65 0.6588320071114566 1104 66-67 0.604411008407375 1104 68-69 0.5515896725015708 1104 70-71 0.6779016627556729 1104 72-73 0.6753290346928722 1104 74-75 0.816309354751354 1104 76-77 0.6668843089164902 1104 78-79 0.4926148077618677 1104 80-81 0.6178297337411145 1104 82-83 0.6097782179423596 1104 84-85 0.6027079616234481 1104 86-87 0.97834086599633 1104 88-89 0.761473854932472 1104 90-91 0.6986422751360095 1104 92-93 0.9443702939384622 1104 94-95 1.058267019181418 1104 96-97 1.153324965111512 1104 98-99 1.3236198265163885 1104 100-101 1.2876641952316312 1105 1 0.10023714629502933 1105 2 0.1167299665916488 1105 3 0.1273126123678665 1105 4 0.05424955966547884 1105 5 0.1339213088672082 1105 6 0.149433020716927 1105 7 0.09766531673107437 1105 8 0.10890842473988727 1105 9 0.11149697237101464 1105 10-11 0.20614202396107117 1105 12-13 0.12781063400198178 1105 14-15 0.0837085835481517 1105 16-17 0.27452497099344697 1105 18-19 0.2592235021818112 1105 20-21 0.3265763204090817 1105 22-23 0.25333454971876535 1105 24-25 0.20522787719377789 1105 26-27 0.1286054123713427 1105 28-29 0.09577922780127324 1105 30-31 0.2589436882530265 1105 32-33 0.13144437468867665 1105 34-35 0.16187315984172557 1105 36-37 0.26820151693829786 1105 38-39 0.280850980643649 1105 40-41 0.4044031149636922 1105 42-43 0.5080596438397436 1105 44-45 0.34925035199857035 1105 46-47 0.23770256024518233 1105 48-49 0.22665756216770916 1105 50-51 0.21117473357927707 1105 52-53 0.39311966817719934 1105 54-55 0.48207788824755227 1105 56-57 0.5065186816278455 1105 58-59 0.5147997822921226 1105 60-61 0.4978660442440699 1105 62-63 0.42617165743495633 1105 64-65 0.5836125271257373 1105 66-67 0.5834446900788564 1105 68-69 0.3027684299382827 1105 70-71 0.3955429350995132 1105 72-73 0.31321906224999907 1105 74-75 0.2503258890225766 1105 76-77 0.2660488363691762 1105 78-79 0.4514148940041949 1105 80-81 0.43208363654331805 1105 82-83 0.4488251054702346 1105 84-85 0.571602442788258 1105 86-87 0.7393829158686529 1105 88-89 0.7618006325013837 1105 90-91 0.8174171624482796 1105 92-93 0.8640692543141562 1105 94-95 0.8857759634540514 1105 96-97 0.8404768796238073 1105 98-99 1.1853972943608007 1105 100-101 1.4371470781509323 1106 1 -0.426694798030816 1106 2 -0.3397515077021396 1106 3 -0.22517494789016723 1106 4 -0.2299975739540372 1106 5 -0.2475273679789609 1106 6 -0.2356998380560995 1106 7 -0.34809585574856783 1106 8 -0.2705491171205239 1106 9 -0.5744610624214488 1106 10-11 -0.5520099854162837 1106 12-13 -0.7182125299231501 1106 14-15 -0.5435063115388346 1106 16-17 -0.5590118747071742 1106 18-19 -0.6327875053402536 1106 20-21 -0.4616068950604486 1106 22-23 -0.5355209163931249 1106 24-25 -0.7546936418132191 1106 26-27 -0.7592048049069007 1106 28-29 -0.7283224326422868 1106 30-31 -0.8242387897591001 1106 32-33 -0.9554797293005848 1106 34-35 -0.9612946466494563 1106 36-37 -0.8764096101294996 1106 38-39 -0.9642189225553679 1106 40-41 -1.1808496776710555 1106 42-43 -1.494984114553688 1106 44-45 -1.511400085430104 1106 46-47 -1.332942365659754 1106 48-49 -1.431590704807391 1106 50-51 -1.4187757403341337 1106 52-53 -1.7717152578053685 1106 54-55 -1.9677132948669964 1106 56-57 -2.1292798294844815 1106 58-59 -2.29788836637929 1106 60-61 -2.2517900677252527 1106 62-63 -2.4138225981600137 1106 64-65 -2.94741308205289 1106 66-67 -2.6480261104893685 1106 68-69 -2.366422152342551 1106 70-71 -2.249034812317447 1106 72-73 -2.2511959011488294 1106 74-75 -2.454403541737939 1106 76-77 -2.344380363838866 1106 78-79 -2.3630745914057947 1106 80-81 -2.4115986823332634 1106 82-83 -2.442941376702702 1106 84-85 -2.242396439381576 1106 86-87 -2.1122224124551465 1106 88-89 -2.3895042789649175 1106 90-91 -2.3807205635436013 1106 92-93 -2.363220550919465 1106 94-95 -2.1216622647851366 1106 96-97 -2.311927231507145 1106 98-99 -2.0892675506567038 1106 100-101 -1.6740723658838128 1107 1 0.04319307113232185 1107 2 0.036552816888637096 1107 3 0.02434128946540426 1107 4 0.034778922120821676 1107 5 0.09105064901694249 1107 6 -0.012787754438939203 1107 7 0.044553504472339966 1107 8 0.010255755442116765 1107 9 0.03531756332573366 1107 10-11 -0.014215081634397109 1107 12-13 0.02303507519253145 1107 14-15 0.12978081774264894 1107 16-17 0.0958962622363444 1107 18-19 0.13190165024067824 1107 20-21 0.19067544365476152 1107 22-23 0.1637242862607522 1107 24-25 0.014136809150876672 1107 26-27 0.2095344769388845 1107 28-29 0.29212002871652487 1107 30-31 0.16421033021563147 1107 32-33 0.17628836134848314 1107 34-35 0.1696794032534754 1107 36-37 0.1660709715661639 1107 38-39 0.2385573884693244 1107 40-41 0.14009712518829076 1107 42-43 0.14035456715522088 1107 44-45 0.018518620111983353 1107 46-47 0.15444112052609427 1107 48-49 0.007562882055317743 1107 50-51 -0.010090426139676367 1107 52-53 0.09576648045391778 1107 54-55 0.15359866304365966 1107 56-57 0.03852399180171773 1107 58-59 0.12518340278018059 1107 60-61 0.05192872300373352 1107 62-63 0.1263580171956349 1107 64-65 0.22669401095237163 1107 66-67 0.24570324086189288 1107 68-69 0.45241531776625976 1107 70-71 0.24238867023120747 1107 72-73 0.445311436250293 1107 74-75 0.4953461619424857 1107 76-77 0.5026836395941352 1107 78-79 0.4325083724918741 1107 80-81 0.6525188117682639 1107 82-83 0.6114653699715795 1107 84-85 0.5841752508125211 1107 86-87 0.7765548525351136 1107 88-89 0.6695957231089231 1107 90-91 0.7996730509490177 1107 92-93 0.9790784104066432 1107 94-95 1.0700026603891892 1107 96-97 0.8950709355389286 1107 98-99 0.9808361572064932 1107 100-101 1.324929001254148 1108 1 0.09027318319152045 1108 2 0.14839761897702886 1108 3 0.10134914885843926 1108 4 0.07476965179650819 1108 5 0.008370377822210173 1108 6 0.07466582788463683 1108 7 -0.023138735858552195 1108 8 -0.032691966119010374 1108 9 -0.023528613458140057 1108 10-11 0.09136432495915159 1108 12-13 0.1511912695885158 1108 14-15 -0.06278525785276656 1108 16-17 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2316 52-53 -0.12151816130816684 2316 54-55 0.019577762824887657 2316 56-57 -0.10380410616702562 2316 58-59 0.2132144396875404 2316 60-61 0.21037118657371678 2316 62-63 0.4036462468011237 2316 64-65 0.40548975337820536 2316 66-67 0.34834339872700326 2316 68-69 0.27673419456507986 2316 70-71 0.44776692996219936 2316 72-73 0.6374868446767721 2316 74-75 0.5060792779432361 2316 76-77 0.7041096011464738 2316 78-79 0.6556351161528333 2316 80-81 0.6508960343238392 2316 82-83 0.5079669133628322 2316 84-85 0.5106801346096965 2316 86-87 0.765438723941859 2316 88-89 1.0467007458834807 2316 90-91 0.8893505780588882 2316 92-93 1.1621728634770356 2316 94-95 1.14071410499103 2316 96-97 1.093584351805056 2316 98-99 0.9874221180165215 2316 100-101 1.5865410357881693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 3.0 5 24.0 6 49.0 7 141.0 8 273.0 9 606.0 10 939.0 11 1638.0 12 2425.0 13 3540.0 14 4641.0 15 6465.0 16 8694.0 17 12531.0 18 11721.0 19 7236.0 20 5846.0 21 6101.0 22 6119.0 23 6666.0 24 7316.0 25 8704.0 26 10364.0 27 12636.0 28 15874.0 29 19831.0 30 24649.0 31 30897.0 32 39847.0 33 52368.0 34 72275.0 35 104651.0 36 161192.0 37 244114.0 38 183167.0 39 14356.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68112243725028 19.678607390650647 15.710259275096442 21.930010897002635 2 15.715313900912486 20.471786650881004 40.43562547166256 23.37727397654395 3 19.630262513247914 24.604125168787405 30.238246467758735 25.527365850205946 4 12.981348589539362 17.29173541488381 37.04250455231927 32.68441144325756 5 13.380203358793782 37.47208895499393 34.36416134491479 14.783546341297498 6 31.56832339529022 36.676296779044534 17.205422890008062 14.549956935657185 7 28.06655348297235 33.31654268241679 20.34153654678203 18.275367287828832 8 26.19332351018975 35.91446695351716 19.14345619616383 18.748753340129255 9 26.073000725245816 17.07986068662119 20.4312500976646 36.4158884904684 10-11 24.9525924550536 27.288867522683464 26.6561847657934 21.102355256469533 12-13 25.702672988639936 24.496436076429937 27.965079616048595 21.835811318881532 14-15 22.746475571900906 25.73639107019258 26.532598834275355 24.984534523631154 16-17 21.512086970669365 29.15377876969263 27.30559689385214 22.028537365785862 18-19 21.406195566348963 27.164684113623682 30.407424531692506 21.02169578833485 20-21 22.05659785082041 26.076531604135845 31.01396401427149 20.852906530772263 22-23 21.89649078701178 26.020607715736883 30.766246879864617 21.31665461738672 24-25 21.629790046391832 26.297836120183067 30.905766761313235 21.166607072111862 26-27 21.442024682551793 26.425184806607998 30.903929978472167 21.228860532368042 28-29 21.343774330332728 26.598528712894947 30.91578108468587 21.14191587208645 30-31 21.48993434181414 26.377668311721497 30.938122468188915 21.19427487827544 32-33 21.3819287861922 26.39623607532629 30.81867291965214 21.403162218829365 34-35 21.69133023319089 26.424731158086125 30.73594526002682 21.14799334869617 36-37 21.79160864229873 26.248661418046616 30.639761743902273 21.319968195752388 38-39 21.39557885003038 26.614407297682614 30.704554709180105 21.285459143106902 40-41 21.73653869752474 26.468705232209967 30.62591373975037 21.16884233051492 42-43 21.54996182190205 26.51279276579059 30.419002901167687 21.518242511139686 44-45 21.63456986672574 26.659723671232925 30.336784302334667 21.368922159706667 46-47 21.82980714344149 26.973583219889203 29.688567129359406 21.508042507309906 48-49 21.876410391697085 27.02896479202726 29.53938158316841 21.55524323310724 50-51 22.118940313831974 27.318465640904964 29.183101129048715 21.379492916214346 52-53 22.046471824554775 27.497997300319465 28.88892565675451 21.566605218371247 54-55 22.002753911869306 27.46595067409255 28.938365135990484 21.592930278047664 56-57 22.016220136463872 27.68646584680492 28.82718835341624 21.47012566331497 58-59 21.963136554870957 27.355233489627327 29.100419429234297 21.581210526267412 60-61 21.82939350514336 27.485207834860887 29.144724686944752 21.540673973051007 62-63 21.845755197824815 27.400136224879517 29.359678722537865 21.394429854757803 64-65 21.83104805833587 27.384188170496195 29.219363419851145 21.565400351316793 66-67 21.759718431814484 27.509934213126673 29.02853828498208 21.70180907007676 68-69 21.81923638693381 27.435984877383817 29.05087475308092 21.693903982601455 70-71 21.90610042954039 27.342732421061726 29.031893351177995 21.719273798219884 72-73 21.880914453165573 27.401652898639313 28.89548463241803 21.821948015777085 74-75 21.801771658790685 27.773835447331436 28.522245008075135 21.90214788580274 76-77 21.93404399456939 27.5507924850194 28.688757402976538 21.826406117434672 78-79 21.90371051937344 27.594776024053523 28.64734761335298 21.854165843220056 80-81 21.898241301875988 27.729622109242797 28.5761558662643 21.795980722616914 82-83 21.968176463856466 27.705485448874008 28.466535006946835 21.859803080322692 84-85 21.925729694104586 27.728261074386584 28.474282439313374 21.871726792195453 86-87 21.899941814879398 27.606403856211976 28.603088315453434 21.890566013455196 88-89 21.870343696657894 27.60428970491044 28.500689856761653 22.024676741670014 90-91 21.932007471989234 27.581917933141874 28.508608231690502 21.97746636317839 92-93 21.81531384953092 27.423157743791123 28.62485499293119 22.136673413746763 94-95 21.691422152812695 27.528777735596883 28.59853829417404 22.181261817416377 96-97 21.744965332514635 27.58222899567702 28.497104991511225 22.17570068029712 98-99 21.690812508100446 27.376199900174925 28.492383971732778 22.440603619991855 100-101 21.530014456214218 27.275865476767784 28.91564603649217 22.278474030525825 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 341.0 1 251.0 2 239.0 3 351.0 4 278.0 5 206.0 6 431.5 7 1073.0 8 1865.0 9 2252.5 10 1991.5 11 1649.0 12 1669.0 13 1796.0 14 1918.0 15 2050.0 16 2135.5 17 2217.0 18 2417.5 19 2512.5 20 2612.0 21 2964.5 22 3454.5 23 4115.0 24 4908.5 25 6054.0 26 7512.0 27 9093.5 28 10371.5 29 11685.5 30 13424.5 31 15167.0 32 17243.0 33 19486.0 34 21735.5 35 23908.5 36 26762.0 37 29458.5 38 31365.5 39 32941.5 40 33924.5 41 35434.0 42 36986.0 43 38175.5 44 39751.0 45 40657.0 46 41401.5 47 42884.5 48 43470.5 49 42724.5 50 41164.5 51 38859.0 52 36701.5 53 35426.0 54 34445.0 55 31678.0 56 27474.0 57 23778.0 58 21033.5 59 18484.0 60 15104.5 61 12112.0 62 9507.0 63 6912.0 64 5054.0 65 3696.5 66 2624.0 67 1953.0 68 1442.0 69 1004.5 70 701.5 71 476.0 72 345.0 73 246.0 74 171.5 75 133.5 76 92.5 77 52.5 78 34.0 79 21.5 80 12.0 81 7.5 82 4.0 83 1.5 84 2.5 85 2.5 86 0.5 87 1.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041547669056270435 2 0.0 3 0.0 4 0.0 5 0.006985891257249011 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00560709693016039 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.2171867632067814E-4 22-23 0.0012409148943797585 24-25 0.0 26-27 0.0011949550834768046 28-29 0.0014707139488945287 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.838392436118161E-4 38-39 0.0 40-41 1.3787943270886208E-4 42-43 0.02219858866612679 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 4.595981090295402E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3787943270886208E-4 84-85 2.2979905451477013E-4 86-87 0.0 88-89 0.0 90-91 0.010203078020455792 92-93 0.0026656690323713333 94-95 0.0 96-97 0.0 98-99 3.676784872236322E-4 100-101 0.01282278724192417 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1087907.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.7708512470626 #Duplication Level Percentage of deduplicated Percentage of total 1 82.66739138447936 54.371047017312954 2 10.669835084585246 14.035282723578915 3 2.9162973872379356 5.754220849446706 4 1.1995398456749549 3.155790270192475 5 0.6722226005177894 2.2106326331784554 6 0.3993068796021569 1.575765202814532 7 0.26750760236505444 1.2315941905827257 8 0.18941378659929853 0.9966324786052256 9 0.14359641523505537 0.8500012619432633 >10 0.8043043266831797 9.49588766342017 >50 0.04875188182586294 2.1421064198587945 >100 0.020853129496329715 2.332977120695283 >500 4.198610133423737E-4 0.18547817249919724 >1k 2.799073422282491E-4 0.5012973337124706 >5k 2.799073422282491E-4 1.1612866621587457 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6584 0.6051987899700986 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6032 0.5544591587332374 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3851 0.3539824635745519 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1595 0.14661179678042333 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 4.595981090295402E-5 0.0 0.0 0.0 0.0 14-15 9.191962180590804E-5 0.0 0.0 0.0 0.0 16-17 1.3787943270886205E-4 0.0 0.0 0.0 0.0 18-19 1.838392436118161E-4 0.0 0.0 4.595981090295402E-5 0.0 20-21 1.838392436118161E-4 0.0 0.0 1.838392436118161E-4 1.3787943270886205E-4 22-23 1.838392436118161E-4 0.0 0.0 3.2171867632067814E-4 1.838392436118161E-4 24-25 1.838392436118161E-4 0.0 0.0 3.676784872236322E-4 1.838392436118161E-4 26-27 1.838392436118161E-4 0.0 0.0 5.055579199324942E-4 1.838392436118161E-4 28-29 3.676784872236322E-4 0.0 0.0 0.0017924326252152067 1.838392436118161E-4 30-31 4.595981090295402E-4 0.0 0.0 0.004320222224877678 1.838392436118161E-4 32-33 4.595981090295402E-4 0.0 0.0 0.007261650122666736 1.838392436118161E-4 34-35 5.515177308354482E-4 0.0 0.0 0.010065198587746932 1.838392436118161E-4 36-37 5.515177308354482E-4 0.0 0.0 0.015258657219780734 1.838392436118161E-4 38-39 5.515177308354482E-4 0.0 0.0 0.021876869989806112 1.838392436118161E-4 40-41 5.515177308354482E-4 0.0 0.0 0.031528430279426464 1.838392436118161E-4 42-43 5.515177308354482E-4 0.0 0.0 0.040122914918278865 1.838392436118161E-4 44-45 6.434373526413563E-4 0.0 0.0 0.05064771161505533 1.838392436118161E-4 46-47 0.0010111158398649885 0.0 0.0 0.06760688183824537 1.838392436118161E-4 48-49 0.0011030354616708964 0.0 0.0 0.07900491494217796 1.838392436118161E-4 50-51 0.0011030354616708964 0.0 0.0 0.09449337121647347 1.838392436118161E-4 52-53 0.0011030354616708964 0.0 0.0 0.11053334522160442 1.838392436118161E-4 54-55 0.0011030354616708964 0.0 0.0 0.1262516005504147 1.838392436118161E-4 56-57 0.0011030354616708964 0.0 0.0 0.17087857693718306 1.838392436118161E-4 58-59 0.0011030354616708964 0.0 0.0 0.2142186786186687 1.838392436118161E-4 60-61 0.0011030354616708964 0.0 0.0 0.24689610417066898 1.838392436118161E-4 62-63 0.0011030354616708964 0.0 0.0 0.2902821656630576 1.838392436118161E-4 64-65 0.0011030354616708964 0.0 0.0 0.3313242767993955 1.838392436118161E-4 66-67 0.0011030354616708964 0.0 0.0 0.3921750664349067 1.838392436118161E-4 68-69 0.0011030354616708964 0.0 0.0 0.4670895582067217 1.838392436118161E-4 70-71 0.0011489952725738505 0.0 0.0 0.5141064447604438 1.838392436118161E-4 72-73 0.0012409148943797587 0.0 0.0 0.5654895133499462 2.297990545147701E-4 74-75 0.0013328345161856664 0.0 0.0 0.6522156765238205 2.757588654177241E-4 76-77 0.0013787943270886205 0.0 0.0 0.7586126387641591 2.757588654177241E-4 78-79 0.0013787943270886205 0.0 0.0 0.8654691991135272 2.757588654177241E-4 80-81 0.0015626335707004367 0.0 0.0 0.9520574828546926 2.757588654177241E-4 82-83 0.0015626335707004367 0.0 0.0 1.0642913410797061 2.757588654177241E-4 84-85 0.0016085933816033906 0.0 0.0 1.2461083530117922 2.757588654177241E-4 86-87 0.0016545531925063446 0.0 0.0 1.4225480670682327 2.757588654177241E-4 88-89 0.0016545531925063446 0.0 0.0 1.640397570748235 2.757588654177241E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3425 0.0 46.3485 1 GTATCAA 5490 0.0 38.3514 1 ATCAACG 6855 0.0 30.35127 3 TATCAAC 6885 0.0 30.21902 2 TCAACGC 7005 0.0 29.70135 4 CAACGCA 7035 0.0 29.574692 5 AACGCAG 7285 0.0 28.622349 6 CTATACG 70 0.008367263 27.158875 1 ACTGCGC 115 1.281759E-4 24.782417 8 ACGCAGA 8585 0.0 24.233942 7 CGCAGAG 8580 0.0 24.137342 8 GAGTACT 5560 0.0 23.23723 12-13 TACATGG 4610 0.0 22.77202 2 GTATAGG 355 0.0 22.759901 1 GTACATG 4725 0.0 22.531807 1 GCAGAGT 9145 0.0 22.386377 9 ACATGGG 4500 0.0 22.061954 3 GTACTTT 5830 0.0 22.037838 14-15 TACCTGG 845 0.0 21.923916 2 TGTACCG 160 4.987502E-5 20.782045 5 >>END_MODULE