FastQCFastQC Report
Thu 26 May 2016
SRR937632_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937632_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962271
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48220.5011062372242331No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41040.42649108203406316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25700.2670765304160678No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12780.13281081940534423No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10100.10496003724522511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27550.047.639351
GTATCAA45900.039.7829441
ATCAACG57600.031.2778283
TCAACGC58500.030.959154
TATCAAC59100.030.6464392
CAACGCA59950.030.208775
AACGCAG61050.029.86
CGCAGAG68150.026.4165748
ACGCAGA69300.026.1152927
GCAGAGT71550.024.4310059
GAGTACT39750.024.13763612-13
GTACCTG9150.023.906451
AGTACGT1201.7012369E-423.769092
GTACATG43150.023.3632581
TACATGG42100.022.8092922
GTACTTT42800.022.30540814-15
GAGTACG1503.0065163E-522.1914221
AGAGTAC64700.021.69370510-11
CCGTCCG1100.002876658221.5913839
ACATGGG43750.021.4046463
Produced by FastQC (version 0.11.5)