FastQCFastQC Report
Thu 26 May 2016
SRR937632_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937632_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962271
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59560.6189524572599611No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53820.5593019014394074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35690.37089343854278056No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.14455387307733475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC452.0469088E-763.3351363
GGTATCA31900.042.0005231
GTATCAA49950.034.432561
GGACCGA2300.030.9775336
CGCGGAA701.7076673E-830.53499682-83
ATCAACG62300.027.4486143
TCAACGC62750.027.327474
TATCAAC62800.027.2300722
CAACGCA63500.027.0047075
AACGCAG65650.026.2636626
GTACATG39350.024.7517191
AGGACCG3200.023.7506755
CGCGCTT801.8610244E-623.74944362-63
TACATGG39350.023.5394252
GAGTACT47900.022.90655912-13
CGCAGAG74700.022.700278
ACATGGG38400.022.6373633
ACGCAGA75550.022.6334847
ATTCGCG953.290079E-722.49947280-81
GATCGGA1503.0331023E-522.1661457
Produced by FastQC (version 0.11.5)