##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937632_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 962271 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24459014144664 33.0 31.0 34.0 31.0 34.0 2 32.470542082220085 34.0 31.0 34.0 31.0 34.0 3 32.56418618040033 34.0 31.0 34.0 31.0 34.0 4 36.17827514286516 37.0 35.0 37.0 35.0 37.0 5 36.022129940526106 37.0 35.0 37.0 35.0 37.0 6 36.12152917421392 37.0 35.0 37.0 35.0 37.0 7 36.08884815192394 37.0 35.0 37.0 35.0 37.0 8 36.08354715043891 37.0 35.0 37.0 35.0 37.0 9 37.76526467076322 39.0 38.0 39.0 35.0 39.0 10-11 37.710109730003296 39.0 37.0 39.0 35.0 39.0 12-13 37.627828854865214 39.0 37.0 39.0 35.0 39.0 14-15 38.86756901122449 40.0 38.0 41.0 35.0 41.0 16-17 38.831861814395324 40.0 38.0 41.0 35.0 41.0 18-19 38.829485145037104 40.0 38.0 41.0 35.0 41.0 20-21 38.75386975186824 40.0 38.0 41.0 35.0 41.0 22-23 38.60756741084373 40.0 38.0 41.0 34.0 41.0 24-25 38.555631417760694 40.0 38.0 41.0 34.0 41.0 26-27 38.48552123050575 40.0 38.0 41.0 34.0 41.0 28-29 38.37290482618722 40.0 38.0 41.0 34.0 41.0 30-31 38.426841295227646 40.0 38.0 41.0 34.0 41.0 32-33 38.33050720638988 40.0 38.0 41.0 34.0 41.0 34-35 38.19021356769559 40.0 38.0 41.0 34.0 41.0 36-37 38.027492255300224 40.0 37.5 41.0 33.5 41.0 38-39 37.88959814854651 40.0 37.0 41.0 33.0 41.0 40-41 37.70465284727483 40.0 37.0 41.0 33.0 41.0 42-43 37.487334129366886 40.0 37.0 41.0 33.0 41.0 44-45 37.174479954191696 40.0 37.0 41.0 32.0 41.0 46-47 36.79011266057067 40.0 36.0 41.0 31.0 41.0 48-49 36.42310793944741 39.5 36.0 41.0 30.0 41.0 50-51 36.157870288099716 39.0 36.0 41.0 30.0 41.0 52-53 35.93124234233392 39.0 35.0 40.5 29.0 41.0 54-55 35.72559601193427 39.0 35.0 40.0 28.5 41.0 56-57 35.440359836262346 39.0 35.0 40.0 28.0 41.0 58-59 35.183094990912124 38.0 35.0 40.0 27.5 41.0 60-61 34.83781543868619 38.0 34.0 40.0 26.5 41.0 62-63 34.47597506315788 37.5 34.0 40.0 26.0 41.0 64-65 34.06996106086539 37.0 34.0 40.0 26.0 41.0 66-67 33.63164378849618 36.5 33.0 39.0 25.0 40.5 68-69 33.16831848824292 36.0 33.0 39.0 23.0 40.0 70-71 32.745441772639936 35.5 32.0 38.5 22.5 40.0 72-73 32.298449189469494 35.0 32.0 38.0 21.5 40.0 74-75 31.795942619075085 35.0 31.0 37.0 20.0 39.0 76-77 30.579127397583427 34.0 29.5 36.0 19.5 38.5 78-79 31.133348090091047 34.0 31.0 36.0 19.5 38.5 80-81 30.9203924881868 34.0 31.0 36.0 18.0 37.5 82-83 30.559690045735557 34.0 31.0 35.0 17.5 37.0 84-85 30.150060637803698 34.0 30.0 35.0 11.5 36.5 86-87 29.712294145827943 34.0 30.0 35.0 7.0 36.0 88-89 29.258609580876907 34.0 29.0 35.0 2.0 36.0 90-91 28.736840765231417 34.0 29.0 35.0 2.0 35.0 92-93 28.267873083570013 33.5 29.0 35.0 2.0 35.0 94-95 27.7802183584458 33.0 27.0 35.0 2.0 35.0 96-97 27.21249315421539 33.0 26.5 35.0 2.0 35.0 98-99 26.370996839767592 33.0 25.0 34.5 2.0 35.0 100-101 23.923364104290787 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15977894084375777 1101 2 0.2724853518186947 1101 3 0.104344895569362 1101 4 0.06915049196983603 1101 5 0.05413153994942377 1101 6 0.051111335103897204 1101 7 0.006115257653604544 1101 8 0.027027337965471077 1101 9 0.1318536594704014 1101 10-11 0.1228797798106811 1101 12-13 0.2002322904197129 1101 14-15 0.0708675265785601 1101 16-17 0.013737456774236989 1101 18-19 0.07251535443469237 1101 20-21 0.23253824238049248 1101 22-23 0.15284445182314244 1101 24-25 0.008426300070134118 1101 26-27 -0.027133840343935844 1101 28-29 -0.009677594387476063 1101 30-31 0.020076369191265542 1101 32-33 0.15782354480287353 1101 34-35 0.1786353577652946 1101 36-37 0.15975170642970227 1101 38-39 0.29868151294622436 1101 40-41 -0.04851902750721848 1101 42-43 0.0034422709981996036 1101 44-45 0.09801698081756172 1101 46-47 0.07236538451691388 1101 48-49 0.2691538996688152 1101 50-51 0.20913248864654577 1101 52-53 0.10984348061291627 1101 54-55 0.09043834155480823 1101 56-57 0.08912434988240392 1101 58-59 0.04634661162580045 1101 60-61 0.07829438996319737 1101 62-63 0.3369998268700769 1101 64-65 0.1482830674942477 1101 66-67 0.29562850535984353 1101 68-69 0.29390703893401593 1101 70-71 0.03393994548996204 1101 72-73 0.23308075421162044 1101 74-75 0.22863464375186027 1101 76-77 0.022105244646937194 1101 78-79 0.0038520923850278166 1101 80-81 0.2169340273051894 1101 82-83 0.21288479335570543 1101 84-85 0.28771495388661705 1101 86-87 0.5229121948398969 1101 88-89 0.6629926657427738 1101 90-91 0.6269696687806707 1101 92-93 0.7436757248115029 1101 94-95 0.8865860208349545 1101 96-97 0.8429300577610732 1101 98-99 1.323143214021787 1101 100-101 2.096161171264722 1102 1 0.23315666809187263 1102 2 0.2981421471555308 1102 3 0.18785670687717726 1102 4 0.1837113350932924 1102 5 0.04715844699289562 1102 6 0.07687063346348566 1102 7 0.06268898584013272 1102 8 0.13101809825637645 1102 9 0.24758967809419374 1102 10-11 0.1489516402044515 1102 12-13 0.2700213290929483 1102 14-15 0.27306267934054773 1102 16-17 0.32478793229442005 1102 18-19 0.29722616236784916 1102 20-21 0.2716539733461474 1102 22-23 0.38820691189568635 1102 24-25 0.21900179679472842 1102 26-27 0.12655067412017473 1102 28-29 0.06414876404827652 1102 30-31 0.20352790583429936 1102 32-33 0.31615570512625624 1102 34-35 0.3894754133479168 1102 36-37 0.3335221213142958 1102 38-39 0.3032248511520592 1102 40-41 0.29707063556823243 1102 42-43 0.5718446314551358 1102 44-45 0.5005178380171387 1102 46-47 0.5136297087729531 1102 48-49 0.7012965376779405 1102 50-51 0.9023701765537169 1102 52-53 0.8619467776731682 1102 54-55 0.5994113262334935 1102 56-57 0.895057975565912 1102 58-59 1.0161464780831224 1102 60-61 0.7331562454844658 1102 62-63 0.7652473500144623 1102 64-65 0.791497475665615 1102 66-67 0.8813192277535009 1102 68-69 0.8951995154287005 1102 70-71 0.8478872141814264 1102 72-73 0.8739458941607339 1102 74-75 1.0824382638410128 1102 76-77 1.0461757696140026 1102 78-79 1.1613876887252381 1102 80-81 1.0564121281118624 1102 82-83 1.093387924389937 1102 84-85 1.3283642600061185 1102 86-87 1.3844107601585023 1102 88-89 1.4084736900511494 1102 90-91 1.6761989110162467 1102 92-93 1.8058261227325687 1102 94-95 1.7150186990873486 1102 96-97 2.0147879250485303 1102 98-99 2.017784543673674 1102 100-101 2.733816595082242 1103 1 0.10250839869117101 1103 2 0.15051615391964646 1103 3 0.1422546258284214 1103 4 -0.015087022496466318 1103 5 0.011364692268486465 1103 6 0.00412477763928365 1103 7 -0.11145618363545395 1103 8 -0.039896903109926996 1103 9 0.118685316196121 1103 10-11 0.05986188610463472 1103 12-13 0.09262256906299626 1103 14-15 0.0854620249316298 1103 16-17 -0.05852818909062307 1103 18-19 0.009059465535912636 1103 20-21 0.10834345915250054 1103 22-23 0.0699672151098909 1103 24-25 0.012478645837070701 1103 26-27 -0.11544328572807672 1103 28-29 -0.03502790151824087 1103 30-31 -0.04733215925170953 1103 32-33 0.15018177501741548 1103 34-35 0.18608483697833123 1103 36-37 0.1529538786009823 1103 38-39 0.018583587191749018 1103 40-41 -0.11107043852831566 1103 42-43 0.11995433367068387 1103 44-45 -0.07081770270420407 1103 46-47 0.1533303983155463 1103 48-49 0.0180918503498404 1103 50-51 0.09041798673253965 1103 52-53 0.010804933475014877 1103 54-55 0.05645988522079648 1103 56-57 0.23387641967676842 1103 58-59 0.058037166241661 1103 60-61 -0.02178038970385643 1103 62-63 0.14287359167021663 1103 64-65 0.2911692065488225 1103 66-67 0.2585128639461516 1103 68-69 0.09693561544963814 1103 70-71 0.382240488532787 1103 72-73 0.4555912144626717 1103 74-75 0.22459476126276812 1103 76-77 0.10235272973551446 1103 78-79 0.2571343856281523 1103 80-81 0.41670256643098114 1103 82-83 0.29452994603085614 1103 84-85 0.3731774305179698 1103 86-87 0.43130888316892424 1103 88-89 0.5455120283351356 1103 90-91 0.4154268930306486 1103 92-93 0.7544511187401071 1103 94-95 0.7653396930507448 1103 96-97 1.0651912524552785 1103 98-99 1.397545421802434 1103 100-101 2.251373492105106 1104 1 0.15044682868368398 1104 2 0.14111716807908437 1104 3 0.08782876199179412 1104 4 0.032151055588265365 1104 5 -0.0674127745496591 1104 6 -0.010308983340522104 1104 7 -0.039804849433892286 1104 8 -0.04686162920375381 1104 9 0.09603852631554588 1104 10-11 -0.0533665214433654 1104 12-13 -0.046915448061334075 1104 14-15 0.14926297535973276 1104 16-17 0.07210683820300545 1104 18-19 0.04567831061336136 1104 20-21 0.05122113656113214 1104 22-23 0.14306531979553228 1104 24-25 -0.1057374310838739 1104 26-27 -0.07671922853613466 1104 28-29 -0.0995240083652078 1104 30-31 -0.1658613509721789 1104 32-33 -0.06822584226208761 1104 34-35 0.053046376505612614 1104 36-37 0.04083314929287951 1104 38-39 -0.05256690187694346 1104 40-41 -0.06841616714368115 1104 42-43 -0.0742003949169785 1104 44-45 -0.21973946556823165 1104 46-47 -0.06530616684281654 1104 48-49 0.09683883696626339 1104 50-51 0.12041119796033684 1104 52-53 0.11099889839507426 1104 54-55 0.19424875440530798 1104 56-57 0.19269628168458297 1104 58-59 0.395271157208434 1104 60-61 0.2399686889627759 1104 62-63 0.3771129028176574 1104 64-65 0.3127166309717708 1104 66-67 0.26488946069308383 1104 68-69 0.10764594269616623 1104 70-71 0.28926825322562877 1104 72-73 0.3954648601718844 1104 74-75 0.3557947113860749 1104 76-77 0.18932625352392662 1104 78-79 0.45112805405897305 1104 80-81 0.40889894205790256 1104 82-83 0.46965948688818315 1104 84-85 0.5617303829411462 1104 86-87 0.7145149847511263 1104 88-89 0.50321001109997 1104 90-91 0.5967259038095527 1104 92-93 0.7959492206548191 1104 94-95 0.805528531755062 1104 96-97 0.9491953811371445 1104 98-99 1.0359764534187157 1104 100-101 1.7710907189609344 1105 1 0.13038011701553387 1105 2 0.15323716394381393 1105 3 0.10030527303844394 1105 4 0.06277259305809224 1105 5 -0.05444748042843628 1105 6 0.007530458641006987 1105 7 0.07906369756529585 1105 8 0.051352602213370346 1105 9 0.056799051495097785 1105 10-11 0.0352924949700153 1105 12-13 0.02548716020069719 1105 14-15 0.2381367370586034 1105 16-17 0.20734353691803875 1105 18-19 0.07909761807691496 1105 20-21 0.1801735900545296 1105 22-23 0.16906546270566736 1105 24-25 0.16267517176581947 1105 26-27 0.10310315240756296 1105 28-29 -0.06352281643385282 1105 30-31 0.07588786910334733 1105 32-33 0.2085739892863856 1105 34-35 0.06524770952271552 1105 36-37 0.08760353223124184 1105 38-39 -0.010011607816529988 1105 40-41 0.028910197039991203 1105 42-43 0.07630982467813396 1105 44-45 0.19072315278872765 1105 46-47 0.3417513134386283 1105 48-49 0.17120379904946503 1105 50-51 0.23274540699834034 1105 52-53 0.31145925147432507 1105 54-55 0.36527608064786676 1105 56-57 0.39930937225312135 1105 58-59 0.4439848899603973 1105 60-61 0.26193466114698793 1105 62-63 0.36598757670888915 1105 64-65 0.36910912302050036 1105 66-67 0.5317338528384994 1105 68-69 0.29472215332747 1105 70-71 0.5503840773306123 1105 72-73 0.7443571545789354 1105 74-75 0.6796070369292835 1105 76-77 0.4473562068804888 1105 78-79 0.6165324745431668 1105 80-81 0.8904779774448848 1105 82-83 0.8869586069266191 1105 84-85 0.8940979326100198 1105 86-87 1.0243388404058074 1105 88-89 1.156954332702508 1105 90-91 0.9174770638141787 1105 92-93 1.0924641897314977 1105 94-95 1.3900059063438128 1105 96-97 1.7172742736139952 1105 98-99 1.812935918630739 1105 100-101 2.1704416940945706 1106 1 -0.1277639489381741 1106 2 0.04120161627678698 1106 3 -0.0943071107136575 1106 4 -0.041318082722639815 1106 5 -0.007899018382723 1106 6 -0.10537670063589388 1106 7 -0.10233540634753524 1106 8 -0.10908820435312805 1106 9 -0.059844592299249655 1106 10-11 0.0846459616511126 1106 12-13 -0.28853293264795354 1106 14-15 -0.08954168579980148 1106 16-17 0.007466266876029692 1106 18-19 0.047756577583953685 1106 20-21 -0.1585219155110238 1106 22-23 -0.07462173613711798 1106 24-25 -0.1036167019086136 1106 26-27 -0.2355743046347527 1106 28-29 -0.11021654708121531 1106 30-31 -0.09885837306205048 1106 32-33 -0.2014567636363651 1106 34-35 -0.3747242228226142 1106 36-37 -0.22077133471593413 1106 38-39 -0.2097246803178905 1106 40-41 -0.10111444579733586 1106 42-43 -0.015091418645013732 1106 44-45 0.3126853882347902 1106 46-47 0.30988114306937575 1106 48-49 0.15805743080852608 1106 50-51 0.16761885163383283 1106 52-53 0.20468242718683172 1106 54-55 -0.023283532743967328 1106 56-57 -0.17881388067184645 1106 58-59 -0.03994620165830298 1106 60-61 0.1145262089795338 1106 62-63 0.08740597933534389 1106 64-65 -0.10439476445532136 1106 66-67 -0.04326788598264386 1106 68-69 -0.16533206267550327 1106 70-71 0.18375165883730205 1106 72-73 0.3527931223803691 1106 74-75 0.08967256627486364 1106 76-77 0.1793325553913121 1106 78-79 0.3713137924727441 1106 80-81 0.45128257180652653 1106 82-83 0.3516812275369716 1106 84-85 0.30732899684779724 1106 86-87 0.23209275011212682 1106 88-89 0.06238843490293178 1106 90-91 0.4051459605739822 1106 92-93 0.7397586280570536 1106 94-95 0.7026644362079573 1106 96-97 0.778717169407102 1106 98-99 0.9483491461733138 1106 100-101 1.6016649349578351 1107 1 -0.007364581003884041 1107 2 0.019219415521583016 1107 3 0.05493835608287867 1107 4 0.08745005628606606 1107 5 -0.20297687340026727 1107 6 -0.013890145374936935 1107 7 0.037583469658173385 1107 8 0.09927808877973376 1107 9 0.23510479097922143 1107 10-11 0.0023916447910110605 1107 12-13 0.08690332802387957 1107 14-15 0.018286417488425855 1107 16-17 -0.12471142440676175 1107 18-19 0.046831161718650094 1107 20-21 0.04967330434910622 1107 22-23 0.04556176496556219 1107 24-25 0.11456986421716664 1107 26-27 -0.1177882119864222 1107 28-29 -0.024954866922477947 1107 30-31 0.038738862317622136 1107 32-33 0.05510453968464191 1107 34-35 0.20445744650942999 1107 36-37 0.06869083760012984 1107 38-39 0.04389406740669699 1107 40-41 0.08838804072171058 1107 42-43 0.2045509381442585 1107 44-45 0.11809692667433325 1107 46-47 0.15913032677823935 1107 48-49 -0.03871209584745117 1107 50-51 0.05709408079577827 1107 52-53 0.2080457563203666 1107 54-55 0.3676808439870243 1107 56-57 0.23398497337549173 1107 58-59 0.15231251831333026 1107 60-61 0.3197507581371326 1107 62-63 0.23591950031874376 1107 64-65 0.3644575101997205 1107 66-67 0.29029497462933307 1107 68-69 0.47193208017592525 1107 70-71 0.5942512690380326 1107 72-73 0.4320071955635143 1107 74-75 0.22149619783164098 1107 76-77 0.4322503315721953 1107 78-79 0.6268329307590399 1107 80-81 0.38869072118446724 1107 82-83 0.25460214879752385 1107 84-85 0.47063868807721576 1107 86-87 0.541811141584958 1107 88-89 0.7725007237775436 1107 90-91 0.716563171593581 1107 92-93 1.225278871886502 1107 94-95 1.0151795659472604 1107 96-97 1.3101636773203111 1107 98-99 1.6735459042633174 1107 100-101 2.3010615561892216 1108 1 0.005301022016652723 1108 2 0.05541401264472512 1108 3 -0.07466636851074071 1108 4 -0.05584772008094063 1108 5 -0.2984565165497699 1108 6 -0.0905791708857393 1108 7 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-1.9213905941793463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 8.0 5 14.0 6 28.0 7 71.0 8 207.0 9 417.0 10 702.0 11 1223.0 12 1937.0 13 2810.0 14 3761.0 15 5334.0 16 7231.0 17 10717.0 18 9842.0 19 5824.0 20 4586.0 21 4738.0 22 4995.0 23 5343.0 24 6009.0 25 7107.0 26 8610.0 27 10699.0 28 13219.0 29 16600.0 30 20892.0 31 26310.0 32 34254.0 33 45245.0 34 62569.0 35 92177.0 36 143829.0 37 221496.0 38 169754.0 39 13706.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.830202213736726 19.595219060549766 15.671538606283587 21.903040119429924 2 15.530448283279865 20.49827959067664 40.69061626090779 23.280655865135707 3 19.527866889888607 24.615311071413355 30.43851472194423 25.4183073167538 4 12.929842975464268 17.307096760784397 37.166595654026416 32.596464609724926 5 13.448770449206975 37.477809882137734 34.35445984991789 14.718959818737398 6 31.481360240514366 36.79244204595171 17.167409180989555 14.558788532544368 7 27.941816806284304 33.39662111816734 20.391864661826034 18.26969741372233 8 26.179735230511987 35.94673433991048 19.076226967247273 18.79730346233026 9 26.03227157422389 17.04831591100636 20.379809845667175 36.53960266910257 10-11 24.884777780895405 27.34510340642085 26.651120110654897 21.118998702028847 12-13 25.75738050426561 24.58520874925044 27.938821343315688 21.718589403168263 14-15 22.68191600910762 25.853735590078053 26.578999055359663 24.885349345454657 16-17 21.47944809726158 29.092843907797285 27.425434207203587 22.00227378773755 18-19 21.303769935912026 27.117153068106592 30.563531479177904 21.015545516803478 20-21 21.98535753998358 26.07033369012855 31.1527949535993 20.791513816288568 22-23 21.809164291634083 25.983159568415225 30.963843214075087 21.2438329258756 24-25 21.511946318210303 26.261323567465446 31.085836200706048 21.14089391361821 26-27 21.394517932775024 26.466659010970684 30.93162718700458 21.20719586924972 28-29 21.231542426243752 26.584673997820712 31.070870536004254 21.112913039931286 30-31 21.350815733328623 26.407125702640542 31.125794091636074 21.11626447239476 32-33 21.268748616553964 26.381445559514937 31.00389599187755 21.345909832053547 34-35 21.60404917117943 26.38238084697554 30.893480111112147 21.12008987073288 36-37 21.708293042341005 26.288346156304282 30.804435966809752 21.198924834544965 38-39 21.401247673472444 26.582220601057287 30.757084023107833 21.259447702362433 40-41 21.61527262070664 26.518724974565377 30.762799668700396 21.103202736027583 42-43 21.482381834064405 26.463962618007443 30.577717451460206 21.475938096467946 44-45 21.4953479840918 26.762783041367765 30.475198774565587 21.26667019997485 46-47 21.737535475972987 27.00304799791327 29.815145629453653 21.44427089666009 48-49 21.78539101770707 27.161007657925886 29.548692624011323 21.50490870035572 50-51 22.00991196866579 27.474848561372006 29.197803945042512 21.317435524919695 52-53 21.949077728144008 27.574834726825774 28.96420497147112 21.5118825735591 54-55 21.883907963557046 27.50753166207856 29.06556469019642 21.542995684167973 56-57 21.886038340550634 27.724778155010387 28.98351919573592 21.405664308703056 58-59 21.83885828420476 27.44091841071798 29.22331650855113 21.496906796526137 60-61 21.84748371300808 27.462845705627625 29.294814038872623 21.39485654249167 62-63 21.729377690899966 27.465028043035694 29.47371374591981 21.331880520144534 64-65 21.70043574003581 27.330242727880194 29.48285877886791 21.48646275321609 66-67 21.706411187700763 27.5128316243553 29.181800137383334 21.598957050560603 68-69 21.689991696725766 27.491839616906255 29.184190316449314 21.633978369918662 70-71 21.76710095181087 27.37420123852844 29.14963664082156 21.70906116883913 72-73 21.718978788675518 27.474697823581558 29.01143027573371 21.79489311200922 74-75 21.73864923566151 27.819271098548228 28.59098797634759 21.851091689442672 76-77 21.813761404011967 27.483733792247715 28.878403277247262 21.824101526493056 78-79 21.880946219931808 27.56234989935268 28.765597217415884 21.79110666329963 80-81 21.77245287450209 27.84080576053939 28.650608820176437 21.736132544782084 82-83 21.819032080393235 27.781963482182753 28.563812651334864 21.83519178608914 84-85 21.754994175237538 27.740574121011647 28.66791163819756 21.83652006555326 86-87 21.79230175283262 27.688873508606203 28.64806275986702 21.87076197869415 88-89 21.757384354303515 27.602723141401952 28.59417981005351 22.04571269424102 90-91 21.803534506097748 27.640525691397677 28.670301740959125 21.88563806154545 92-93 21.666083831601025 27.43968436493408 28.77935631106453 22.11487549240037 94-95 21.519873299725337 27.57674293416304 28.739149366446664 22.16423439966496 96-97 21.616259868581718 27.548684310345006 28.672432194257127 22.162623626816146 98-99 21.552486075886225 27.49079130118953 28.56663335998907 22.390089262935177 100-101 21.329063075853366 27.390275411860205 29.007263872187444 22.273397640098985 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 321.0 1 234.5 2 210.5 3 304.5 4 256.0 5 206.0 6 413.5 7 1029.5 8 1697.0 9 2061.5 10 1914.5 11 1586.5 12 1598.5 13 1717.5 14 1838.5 15 1952.5 16 2014.5 17 2073.5 18 2159.0 19 2237.0 20 2414.0 21 2721.5 22 3179.0 23 3811.0 24 4541.0 25 5506.0 26 6750.0 27 8216.5 28 9361.5 29 10645.5 30 12145.5 31 13696.0 32 15517.5 33 17239.5 34 19252.5 35 21279.5 36 23763.5 37 26093.0 38 27686.0 39 28993.5 40 29770.0 41 31283.5 42 32934.0 43 33823.5 44 35088.0 45 36179.5 46 36653.5 47 37628.5 48 38224.0 49 37524.5 50 36320.0 51 34367.5 52 32522.5 53 31419.5 54 30409.5 55 27583.5 56 23645.5 57 20732.0 58 18375.5 59 16018.0 60 13106.0 61 10472.0 62 8252.0 63 6011.5 64 4307.0 65 3114.0 66 2225.0 67 1710.0 68 1289.0 69 862.5 70 570.0 71 405.5 72 295.0 73 209.0 74 161.0 75 119.5 76 75.0 77 41.5 78 23.5 79 14.5 80 8.5 81 5.0 82 3.5 83 3.5 84 3.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03824286505568598 2 0.0 3 0.0 4 1.0392082895566841E-4 5 0.013613628593192562 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004156833158226737 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.0392082895566841E-4 22-23 3.6372290134483945E-4 24-25 8.313666316453473E-4 26-27 0.002546060309413876 28-29 0.0031695852831478866 30-31 8.313666316453473E-4 32-33 0.0 34-35 0.0 36-37 5.1960414477834206E-5 38-39 0.0 40-41 0.0 42-43 0.010028359994222002 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 5.1960414477834206E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.0784165791133682E-4 74-75 1.0392082895566841E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.0392082895566841E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.007430339270330291 92-93 0.0021303769935912026 94-95 0.0 96-97 0.0 98-99 1.558812434335026E-4 100-101 0.011639132843034862 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 962271.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.92259923236746 #Duplication Level Percentage of deduplicated Percentage of total 1 83.44471850206257 55.84337454371252 2 10.190535185109672 13.639542043128683 3 2.767913069338862 5.557078111481903 4 1.1607190509354604 3.1071334346851094 5 0.6483892617412128 2.16959473550389 6 0.37898446162512134 1.5217575144379525 7 0.26140870489177587 1.2245904995327173 8 0.17903712388319853 0.9585303751480817 9 0.13730816832405138 0.827011756807285 >10 0.7685539735337347 9.246006680378606 >50 0.04355117072665173 1.991183518050294 >100 0.018101107842433916 2.1177098822593474 >500 1.560439970413168E-4 0.09450797238334564 >1k 3.120879940826336E-4 0.5179663458799938 >5k 3.120879940826336E-4 1.1840125866103568 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5956 0.6189524572599611 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5382 0.5593019014394074 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3569 0.37089343854278056 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1391 0.14455387307733475 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 1.0392082895566841E-4 0.0 0.0 0.0 0.0 12-13 2.0784165791133682E-4 0.0 0.0 0.0 0.0 14-15 4.1568331582267365E-4 0.0 0.0 0.0 0.0 16-17 4.1568331582267365E-4 0.0 0.0 1.0392082895566841E-4 0.0 18-19 4.1568331582267365E-4 0.0 0.0 1.0392082895566841E-4 0.0 20-21 4.1568331582267365E-4 0.0 0.0 2.0784165791133682E-4 0.0 22-23 4.1568331582267365E-4 0.0 0.0 3.1176248686700525E-4 0.0 24-25 5.196041447783421E-4 0.0 0.0 7.794062171675131E-4 0.0 26-27 5.196041447783421E-4 0.0 0.0 0.0015588124343350262 0.0 28-29 5.196041447783421E-4 0.0 0.0 0.003065664454192218 0.0 30-31 5.196041447783421E-4 0.0 0.0 0.005403883105694758 0.0 32-33 5.196041447783421E-4 0.0 0.0 0.008001903829586468 0.0 34-35 5.715645592561763E-4 0.0 0.0 0.010547964139000344 0.0 36-37 6.235249737340105E-4 0.0 0.0 0.014444995224837909 0.0 38-39 6.235249737340105E-4 0.0 0.0 0.020160640817399673 0.0 40-41 6.235249737340105E-4 0.0 0.0 0.02966939666684333 0.0 42-43 7.794062171675131E-4 0.0 0.0 0.03808698381225248 0.0 44-45 8.313666316453473E-4 0.0 0.0 0.04795946256304097 0.0 46-47 9.352874606010157E-4 0.0 0.0 0.06495051809729276 0.0 48-49 9.352874606010157E-4 0.0 0.0 0.07887590917735232 0.0 50-51 9.352874606010157E-4 0.0 0.0 0.09321698357323457 0.0 52-53 9.352874606010157E-4 0.0 0.0 0.11020803910748636 0.0 54-55 9.352874606010157E-4 0.0 0.0 0.12829026334577265 1.0392082895566841E-4 56-57 9.352874606010157E-4 0.0 0.0 0.17562620093507963 1.0392082895566841E-4 58-59 9.352874606010157E-4 0.0 0.0 0.21662296795809083 1.0392082895566841E-4 60-61 9.8724787507885E-4 0.0 0.0 0.2477472562303135 1.0392082895566841E-4 62-63 0.0010392082895566842 0.0 0.0 0.2901989148587041 1.0392082895566841E-4 64-65 0.0010392082895566842 0.0 0.0 0.33457310882277447 1.0392082895566841E-4 66-67 0.0011431291185123526 0.0 0.0 0.39941970609111155 1.0392082895566841E-4 68-69 0.0011431291185123526 0.0 0.0 0.4761132778603948 1.0392082895566841E-4 70-71 0.001247049947468021 0.0 0.0 0.5265668403183719 1.0392082895566841E-4 72-73 0.001247049947468021 0.0 0.0 0.5770204027763488 1.0392082895566841E-4 74-75 0.001247049947468021 0.0 0.0 0.6686266135007706 1.0392082895566841E-4 76-77 0.001247049947468021 0.0 0.0 0.791045350010548 1.0392082895566841E-4 78-79 0.001247049947468021 0.0 0.0 0.904526895230138 1.0392082895566841E-4 80-81 0.0014548916053793578 0.0 0.0 0.9927037185990226 1.0392082895566841E-4 82-83 0.0014548916053793578 0.0 0.0 1.1136156030889428 1.0392082895566841E-4 84-85 0.0014548916053793578 0.0 0.0 1.3122602676377029 1.0392082895566841E-4 86-87 0.0014548916053793578 0.0 0.0 1.50690398027167 1.0392082895566841E-4 88-89 0.0014548916053793578 0.0 0.0 1.7397905579613226 1.0392082895566841E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 45 2.0469088E-7 63.335136 3 GGTATCA 3190 0.0 42.000523 1 GTATCAA 4995 0.0 34.43256 1 GGACCGA 230 0.0 30.977533 6 CGCGGAA 70 1.7076673E-8 30.534996 82-83 ATCAACG 6230 0.0 27.448614 3 TCAACGC 6275 0.0 27.32747 4 TATCAAC 6280 0.0 27.230072 2 CAACGCA 6350 0.0 27.004707 5 AACGCAG 6565 0.0 26.263662 6 GTACATG 3935 0.0 24.751719 1 AGGACCG 320 0.0 23.750675 5 CGCGCTT 80 1.8610244E-6 23.749443 62-63 TACATGG 3935 0.0 23.539425 2 GAGTACT 4790 0.0 22.906559 12-13 CGCAGAG 7470 0.0 22.70027 8 ACATGGG 3840 0.0 22.637363 3 ACGCAGA 7555 0.0 22.633484 7 ATTCGCG 95 3.290079E-7 22.499472 80-81 GATCGGA 150 3.0331023E-5 22.166145 7 >>END_MODULE