FastQCFastQC Report
Thu 26 May 2016
SRR937631_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937631_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523718
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105830.6945510914749318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85630.561980628961527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59130.3880639330899812No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24720.16223474422432496No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20130.13211105992053648No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19580.12850146811942892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82550.031.8635161
GTATCAA121500.031.0052011
TATCAAC150400.024.8137862
GTACATG103350.024.7143651
ATCAACG151000.024.652223
TACATGG101700.024.635472
TCAACGC152750.024.3993074
CAACGCA154300.024.1557965
AACGCAG157500.023.7352566
ACATGGG102450.023.2950133
GAGTACT81200.022.93135812-13
AGTACTT84500.022.0920312-13
CATGGGG31700.022.0445234
CGCAGAG175650.021.0670328
ACGCAGA175450.021.037597
GTACTTT88750.021.00664914-15
AGAGTAC145600.020.57016410-11
GCAGAGT180300.019.7603159
CATGGGA71250.019.2154374
TACTTTT98300.018.86917914-15
Produced by FastQC (version 0.11.5)