FastQCFastQC Report
Thu 26 May 2016
SRR937631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937631_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523718
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143240.9400689628920837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125130.8212149492228877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88220.5789785248976517No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30000.1968868255149575No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27620.18126713735743752No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23910.15691879993542113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC651.3285878E-436.537538
GGTATCA102500.033.1474381
GTATCAA148250.030.1942371
GGACCGA3350.026.9387266
TATCAAC182250.024.3503042
GTACCTG11350.024.2828661
ATCAACG183300.024.1848963
TCAACGC184550.024.0468314
AGGACCG4350.024.030265
CAACGCA187150.023.6619835
AACGCAG191500.023.1905546
GTACATG99850.022.9385931
GAGTACT111800.022.92092512-13
TACATGG98950.022.568732
AGTACTT115200.022.07951412-13
GTACTTT116150.021.7550814-15
TACCTGG12650.021.4096412
ACATGGG99400.020.936923
ACGCAGA214350.020.4974947
CGCAGAG213650.020.4757218
Produced by FastQC (version 0.11.5)