##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937631_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1523718 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14217853959853 33.0 31.0 34.0 31.0 34.0 2 32.41548895530538 34.0 31.0 34.0 31.0 34.0 3 32.49547225930257 34.0 31.0 34.0 31.0 34.0 4 36.13793890995578 37.0 35.0 37.0 35.0 37.0 5 36.066337734410176 37.0 35.0 37.0 35.0 37.0 6 36.118797572779215 37.0 35.0 37.0 35.0 37.0 7 36.06699336753914 37.0 35.0 37.0 35.0 37.0 8 36.04933852589521 37.0 35.0 37.0 35.0 37.0 9 37.7351701561575 39.0 37.0 39.0 35.0 39.0 10-11 37.65769486217266 39.0 37.0 39.0 35.0 39.0 12-13 37.55342524010348 39.0 37.0 39.0 35.0 39.0 14-15 38.723686075769926 40.0 38.0 41.0 34.5 41.0 16-17 38.69219993463358 40.0 38.0 41.0 34.5 41.0 18-19 38.73973563349649 40.0 38.0 41.0 35.0 41.0 20-21 38.6758215759084 40.0 38.0 41.0 34.0 41.0 22-23 38.62011408935249 40.0 38.0 41.0 34.0 41.0 24-25 38.645557117524376 40.0 38.0 41.0 34.0 41.0 26-27 38.50349014712697 40.0 38.0 41.0 34.0 41.0 28-29 38.35588015630189 40.0 38.0 41.0 34.0 41.0 30-31 38.31555871887055 40.0 38.0 41.0 34.0 41.0 32-33 38.16886457992884 40.0 37.0 41.0 34.0 41.0 34-35 37.99557759375422 40.0 37.0 41.0 33.5 41.0 36-37 37.82490263946478 40.0 37.0 41.0 33.0 41.0 38-39 37.67978884544253 40.0 37.0 41.0 33.0 41.0 40-41 37.47715358091195 40.0 37.0 41.0 33.0 41.0 42-43 37.20950956804343 40.0 36.0 41.0 32.5 41.0 44-45 36.82728759521119 40.0 36.0 41.0 31.5 41.0 46-47 36.33132410327896 39.5 36.0 41.0 30.5 41.0 48-49 35.88620663403596 39.0 35.0 41.0 29.0 41.0 50-51 35.589012533815314 39.0 35.0 41.0 27.5 41.0 52-53 35.34934515441834 39.0 35.0 40.0 27.0 41.0 54-55 35.12676591075251 39.0 35.0 40.0 26.5 41.0 56-57 34.85625916344101 38.0 35.0 40.0 26.0 41.0 58-59 34.60673431697991 38.0 34.0 40.0 26.0 41.0 60-61 34.28186350755192 38.0 34.0 40.0 24.5 41.0 62-63 33.915938841701674 37.5 34.0 40.0 23.0 41.0 64-65 33.51615193887582 37.0 33.0 39.5 22.0 41.0 66-67 33.08966094776068 36.0 33.0 39.0 21.5 40.5 68-69 32.63885115224733 36.0 32.0 39.0 20.0 40.0 70-71 32.23580281915683 35.0 32.0 38.5 18.5 40.0 72-73 31.749501876331447 35.0 31.0 37.5 17.0 40.0 74-75 31.266360967055583 35.0 31.0 37.0 12.5 39.0 76-77 30.039022312527646 33.5 29.5 35.5 11.0 38.5 78-79 30.617254964501306 34.0 31.0 36.0 8.0 38.5 80-81 30.4191454717999 34.0 31.0 36.0 7.0 37.5 82-83 30.03749447076165 34.0 30.0 35.0 4.0 37.0 84-85 29.64859311237381 34.0 30.0 35.0 2.0 36.5 86-87 29.274378526735262 34.0 29.5 35.0 2.0 36.0 88-89 28.82724493639899 34.0 29.0 35.0 2.0 36.0 90-91 28.279707268667824 34.0 29.0 35.0 2.0 35.0 92-93 27.837131280197518 33.0 27.5 35.0 2.0 35.0 94-95 27.365909899338327 33.0 27.0 35.0 2.0 35.0 96-97 26.798062699265873 33.0 25.5 35.0 2.0 35.0 98-99 25.902623057547395 32.5 24.5 34.0 2.0 35.0 100-101 23.122804875967862 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3975842686760629 1101 2 0.2828627023205712 1101 3 0.3525640515780708 1101 4 0.18922953529909137 1101 5 0.18005095470329735 1101 6 0.20107126012381116 1101 7 0.20889175131463134 1101 8 0.20436466464466463 1101 9 0.15789817892845548 1101 10-11 0.3785772010065571 1101 12-13 0.3673185976371016 1101 14-15 0.3268213077058917 1101 16-17 0.3510433875660439 1101 18-19 0.39846318671244063 1101 20-21 0.4020804516700025 1101 22-23 0.2220074887952066 1101 24-25 0.24111622967910762 1101 26-27 0.20267261391982316 1101 28-29 0.1645890273033075 1101 30-31 0.1330927098450303 1101 32-33 0.28592888646242187 1101 34-35 0.21473887677656478 1101 36-37 0.15863699479483273 1101 38-39 0.17641037346680832 1101 40-41 0.162932936779427 1101 42-43 0.011562887575976788 1101 44-45 0.09474851485977354 1101 46-47 -0.25161452791351024 1101 48-49 -0.362390791238667 1101 50-51 -0.15442019147013042 1101 52-53 -0.15754042074873098 1101 54-55 -0.27233621509650163 1101 56-57 -0.4281084597684597 1101 58-59 -0.5887891997928278 1101 60-61 -0.35049586968251134 1101 62-63 -0.30446262601607543 1101 64-65 -0.21981766819970971 1101 66-67 -0.27057046173742805 1101 68-69 -0.25153546806728144 1101 70-71 -0.29246354822312526 1101 72-73 -0.47210361957359126 1101 74-75 -0.4563311020578418 1101 76-77 -0.44479905606657155 1101 78-79 -0.46982583799450595 1101 80-81 -0.5364494682744017 1101 82-83 -0.4562732765824862 1101 84-85 -0.41032139922178246 1101 86-87 -0.5304317792929218 1101 88-89 -0.39752117877465665 1101 90-91 -0.6514878916923266 1101 92-93 -0.9550911860280493 1101 94-95 -0.8421172718148036 1101 96-97 -0.9376360625701565 1101 98-99 -0.8116277301233268 1101 100-101 -0.4913365983636595 1102 1 0.30077484031919965 1102 2 0.23310617172251114 1102 3 0.20241698990561474 1102 4 0.08157480716091214 1102 5 0.05879829237991885 1102 6 0.037847048336765 1102 7 0.12465749192769948 1102 8 0.17106468276384845 1102 9 0.17584072858242195 1102 10-11 0.1656591350413379 1102 12-13 0.2612188144975178 1102 14-15 0.26605965245582297 1102 16-17 0.3097407664381606 1102 18-19 0.31887609896659086 1102 20-21 0.26577519243463144 1102 22-23 0.3267147158422432 1102 24-25 0.20049108367153679 1102 26-27 0.19280563607423318 1102 28-29 0.11475671521576203 1102 30-31 0.14652198748219547 1102 32-33 0.19149660377581768 1102 34-35 0.11871176409689355 1102 36-37 0.03937715773072625 1102 38-39 0.1855799336948607 1102 40-41 0.2302382951575055 1102 42-43 0.22599800779504875 1102 44-45 0.32455303280403314 1102 46-47 0.07628912817300204 1102 48-49 0.26925732510699874 1102 50-51 0.27192846263921666 1102 52-53 0.4495750052856806 1102 54-55 0.4845032067762034 1102 56-57 0.4523032217487213 1102 58-59 0.3988618361344294 1102 60-61 0.06905039862042628 1102 62-63 0.15739506888194654 1102 64-65 0.16981713339522742 1102 66-67 0.2579854849443066 1102 68-69 0.31207653982311 1102 70-71 0.3014664684260069 1102 72-73 0.04656654633658874 1102 74-75 0.05923715085404879 1102 76-77 0.05866723484546199 1102 78-79 0.11175236565371804 1102 80-81 0.17191629535800246 1102 82-83 0.27126691324729535 1102 84-85 0.24406030929183942 1102 86-87 0.11017963488083993 1102 88-89 0.3101881918059419 1102 90-91 0.5144311212566208 1102 92-93 0.4136789202825568 1102 94-95 0.26754766004453856 1102 96-97 0.14667568766316919 1102 98-99 0.3011958084050086 1102 100-101 0.2479579842284778 1103 1 0.40286848431345135 1103 2 0.36771774458463113 1103 3 0.2946935324866118 1103 4 0.18561461782302757 1103 5 0.14586331899464255 1103 6 0.13986726176808872 1103 7 0.19868833687213083 1103 8 0.2371598919074458 1103 9 0.2626551669823982 1103 10-11 0.2798472549005595 1103 12-13 0.2973902989964756 1103 14-15 0.3798988487660182 1103 16-17 0.30759330759779147 1103 18-19 0.2840125000884868 1103 20-21 0.1942949041760258 1103 22-23 0.22586887791735677 1103 24-25 0.18398512552592905 1103 26-27 0.18808245902646803 1103 28-29 0.21790252161171964 1103 30-31 0.19421796235764788 1103 32-33 0.3038104470287806 1103 34-35 0.31782258673487007 1103 36-37 0.19535984865790113 1103 38-39 0.20625192091075206 1103 40-41 0.4073040304028339 1103 42-43 0.4501843406727417 1103 44-45 0.259442460754272 1103 46-47 0.026719903692168145 1103 48-49 -0.1802098668363783 1103 50-51 -0.04055427090847985 1103 52-53 0.07742608810406182 1103 54-55 0.027227470390890574 1103 56-57 0.054012253913363395 1103 58-59 -0.056018420697945714 1103 60-61 -0.0787868915761365 1103 62-63 -0.16698290235997604 1103 64-65 -0.11396477680586514 1103 66-67 0.04611006836707787 1103 68-69 0.09891480464350622 1103 70-71 0.08958001158931239 1103 72-73 -0.012361223664509424 1103 74-75 0.00956882078828869 1103 76-77 -0.010204727927810353 1103 78-79 0.06945867215802082 1103 80-81 -0.03652508702626278 1103 82-83 -0.02066861369145201 1103 84-85 -0.03950182272090785 1103 86-87 -0.1074847289075187 1103 88-89 -0.05832360175866569 1103 90-91 -0.03234543108088417 1103 92-93 0.057722212477656853 1103 94-95 0.06183014074462179 1103 96-97 0.09914762743162342 1103 98-99 0.19947060097650748 1103 100-101 0.31340412881582225 1104 1 0.21272702149220635 1104 2 0.19391557929088066 1104 3 0.09353804357503037 1104 4 0.04355180695400662 1104 5 -0.019902474714577068 1104 6 -0.012402157807372305 1104 7 0.028505495121578406 1104 8 0.03132322381753738 1104 9 0.10254371818191288 1104 10-11 0.09687707360966158 1104 12-13 0.1323810350209058 1104 14-15 0.08658970241361885 1104 16-17 0.06126412459658326 1104 18-19 0.0956828799381455 1104 20-21 0.06532136623879836 1104 22-23 0.10618306311642556 1104 24-25 0.09975031342978724 1104 26-27 0.010305725926379239 1104 28-29 -0.1261912880752618 1104 30-31 0.0024001149440309177 1104 32-33 0.05990157760212611 1104 34-35 0.09977675974028699 1104 36-37 0.10591947226374998 1104 38-39 0.0010702495761520936 1104 40-41 0.016166768962932565 1104 42-43 0.04841495284147612 1104 44-45 -0.07064803687363508 1104 46-47 -0.11669239919693553 1104 48-49 0.10424482837324689 1104 50-51 0.2903925931764135 1104 52-53 0.13022517542193413 1104 54-55 0.0927002481635597 1104 56-57 0.10785817289343669 1104 58-59 0.02201221754465621 1104 60-61 0.15438516829294002 1104 62-63 0.23605673815027473 1104 64-65 0.08432937222904258 1104 66-67 0.06611763851085328 1104 68-69 0.12513987635873747 1104 70-71 0.050857902262286814 1104 72-73 0.044369782762355925 1104 74-75 0.03450401173087059 1104 76-77 -0.023542865701518423 1104 78-79 -0.17847468343663664 1104 80-81 -0.026004428821160275 1104 82-83 -0.0761075584463633 1104 84-85 -0.024008471798218523 1104 86-87 0.004356875769101265 1104 88-89 -0.07342972189519514 1104 90-91 -0.07793810015752456 1104 92-93 -0.1498589327271027 1104 94-95 -0.08229483157007422 1104 96-97 0.15910491088407142 1104 98-99 0.2884132476868153 1104 100-101 0.2326219226277857 1105 1 0.2682222015972613 1105 2 0.24638702790150546 1105 3 0.227020503031369 1105 4 0.07042664321504333 1105 5 0.07516185824133004 1105 6 0.07503344563794201 1105 7 0.02001985572621834 1105 8 0.0832171508704107 1105 9 0.13000723368149636 1105 10-11 0.09931301848001084 1105 12-13 0.19812990061949876 1105 14-15 0.06458809481497241 1105 16-17 0.2256933043419167 1105 18-19 0.11460295925586905 1105 20-21 -0.028012856733617753 1105 22-23 0.03327565267577626 1105 24-25 -0.0014942589163879916 1105 26-27 0.0800637693868822 1105 28-29 0.022391753410346382 1105 30-31 0.1873145734684698 1105 32-33 0.2056693130704801 1105 34-35 0.3134847293146237 1105 36-37 0.19904794760238076 1105 38-39 0.14209240025036962 1105 40-41 0.2818494471990647 1105 42-43 0.39193115758759944 1105 44-45 0.37212176365827077 1105 46-47 0.4324681192156845 1105 48-49 0.48439750115105085 1105 50-51 0.5915899462174892 1105 52-53 0.6726643069259168 1105 54-55 0.6052135871709439 1105 56-57 0.6184787229313926 1105 58-59 0.48411136112571285 1105 60-61 0.5491581965187535 1105 62-63 0.38521506965746966 1105 64-65 0.48611477967853034 1105 66-67 0.3986656198855627 1105 68-69 0.578217142405606 1105 70-71 0.4493105104594335 1105 72-73 0.45645060543155225 1105 74-75 0.48978893649908883 1105 76-77 0.4045608328911392 1105 78-79 0.41797788816331405 1105 80-81 0.38688197840116345 1105 82-83 0.37760100917967065 1105 84-85 0.3511214593945944 1105 86-87 0.23795698181170266 1105 88-89 0.3419943882462846 1105 90-91 0.37882097739683473 1105 92-93 0.29625482473794307 1105 94-95 0.24653094749267268 1105 96-97 0.4200674438382812 1105 98-99 0.5437701618114978 1105 100-101 0.11286162531575883 1106 1 0.10759020271109421 1106 2 0.14575446128690572 1106 3 0.16341329179899589 1106 4 0.10611558391040177 1106 5 0.08032007649325834 1106 6 0.02828551570644322 1106 7 -6.432060151482233E-4 1106 8 0.00476609424219987 1106 9 0.10641811766619469 1106 10-11 0.002281472154493258 1106 12-13 -0.14327471357073307 1106 14-15 0.12167634189038523 1106 16-17 0.2557421334921557 1106 18-19 0.10752798170592115 1106 20-21 0.18530141113777177 1106 22-23 0.27389337724729046 1106 24-25 0.3458410897026596 1106 26-27 0.3580411783013915 1106 28-29 0.21843265869386386 1106 30-31 0.23744431743647 1106 32-33 0.2456702863192035 1106 34-35 -0.1272692050208235 1106 36-37 0.2470872344093209 1106 38-39 0.24587495846167684 1106 40-41 0.07972506150600367 1106 42-43 0.083025391043833 1106 44-45 0.21480061275561013 1106 46-47 9.584707612404486E-4 1106 48-49 0.017835353587614122 1106 50-51 0.12949325305532255 1106 52-53 0.1382169342243742 1106 54-55 -0.06759979103396319 1106 56-57 0.0951691906359784 1106 58-59 0.21925996854646002 1106 60-61 0.2135070142693678 1106 62-63 0.07838714408756431 1106 64-65 -0.1249173551019993 1106 66-67 -0.2797923756456058 1106 68-69 0.1390760524301129 1106 70-71 -0.05131804600994627 1106 72-73 0.03603464072803675 1106 74-75 0.14133345068801972 1106 76-77 0.2941935261181854 1106 78-79 0.05027685935830917 1106 80-81 0.04687866214624137 1106 82-83 -0.13623849759950346 1106 84-85 -0.18221095776323892 1106 86-87 0.2285845064571994 1106 88-89 0.12006348980704473 1106 90-91 0.022307127301473884 1106 92-93 0.00920525078208101 1106 94-95 -0.007168411469844926 1106 96-97 -0.10050415054589124 1106 98-99 -0.11851207941811026 1106 100-101 -0.4274691711125982 1107 1 0.22723977114034 1107 2 0.13405299655538983 1107 3 0.1979738831253215 1107 4 0.18517744849443574 1107 5 0.11206850405772428 1107 6 0.15998111547443017 1107 7 0.16197153272444353 1107 8 0.08504709499472796 1107 9 0.2804540103804811 1107 10-11 0.1913107560036451 1107 12-13 0.19171906568670494 1107 14-15 0.0711850433831458 1107 16-17 0.08828966233721047 1107 18-19 0.28700449058637645 1107 20-21 0.2645405183695715 1107 22-23 0.26459223508876306 1107 24-25 0.25606725735867286 1107 26-27 0.145745454765418 1107 28-29 0.3248694561650325 1107 30-31 0.17513899864767524 1107 32-33 0.13889215659569487 1107 34-35 0.10086601688826846 1107 36-37 0.2046544389132663 1107 38-39 0.21278168613546455 1107 40-41 0.1742801328666559 1107 42-43 0.34302569291695306 1107 44-45 0.07139664829901449 1107 46-47 0.1031224698038713 1107 48-49 -0.11139092403647055 1107 50-51 -0.13886656245707485 1107 52-53 0.042134600486384954 1107 54-55 0.0684121631416943 1107 56-57 -0.017788066286080095 1107 58-59 -0.01245978107134249 1107 60-61 0.10785573378826996 1107 62-63 -0.02615928076435381 1107 64-65 0.007529908741581437 1107 66-67 0.12673800249360312 1107 68-69 0.20643903976653633 1107 70-71 0.2460453078866962 1107 72-73 0.25455390993633387 1107 74-75 0.09712326937008342 1107 76-77 0.09044447755758256 1107 78-79 0.07944966231493922 1107 80-81 0.24462108243556457 1107 82-83 0.2264128971621382 1107 84-85 0.24542035165455545 1107 86-87 0.17669294790782786 1107 88-89 0.28570946594613034 1107 90-91 0.3129070138834251 1107 92-93 0.17858733806222205 1107 94-95 0.21665627787036712 1107 96-97 0.2410458816898462 1107 98-99 0.05776112438290326 1107 100-101 0.13645397199035614 1108 1 0.1525321029161475 1108 2 0.05820604479112035 1108 3 0.0678903936445181 1108 4 0.09648924533669856 1108 5 0.08265546040759375 1108 6 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-0.5215414522570612 2316 68-69 -0.5052840137068699 2316 70-71 -0.4122535373655225 2316 72-73 -0.3066699104748203 2316 74-75 -0.30695741826919587 2316 76-77 -0.4316703412232279 2316 78-79 -0.4732249727866922 2316 80-81 -0.4614005847616873 2316 82-83 -0.548911966091417 2316 84-85 -0.5946446692312328 2316 86-87 -0.5534462585286626 2316 88-89 -0.40937248545220584 2316 90-91 -0.5146390747340597 2316 92-93 -0.33432036893861294 2316 94-95 -0.35607257345947474 2316 96-97 -0.1152213020568631 2316 98-99 -0.12725223673383468 2316 100-101 0.09927533820071943 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 11.0 5 25.0 6 58.0 7 147.0 8 352.0 9 743.0 10 1372.0 11 2306.0 12 3682.0 13 5423.0 14 7264.0 15 10699.0 16 15520.0 17 22352.0 18 19849.0 19 10707.0 20 8165.0 21 8054.0 22 8167.0 23 8685.0 24 9850.0 25 11664.0 26 13981.0 27 17265.0 28 21492.0 29 26874.0 30 33470.0 31 43009.0 32 54930.0 33 72511.0 34 100236.0 35 145277.0 36 219368.0 37 335015.0 38 262605.0 39 22581.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34285783677587 20.252533905197385 15.915438688388573 20.489169569638168 2 15.50221235162937 21.36799591525466 40.58211558831752 22.547676144798448 3 19.492517644341014 26.08245095221032 30.773870230580723 23.651161172867948 4 12.321505685435232 17.69179073818121 37.96069876446954 32.026004811914014 5 12.53867001412867 38.89146397188972 34.0538386390256 14.516027374956012 6 29.96466537771425 37.85365796033125 17.251289280562414 14.930387381392096 7 27.062225424914583 34.85461220514557 20.408172640869243 17.674989729070603 8 26.561607856571882 35.19581707376299 19.111410379085893 19.131164690579226 9 26.718592285449144 16.822469774590836 20.784029590777294 35.67490834918272 10-11 24.684882635763312 28.05811180284016 26.317336935049664 20.93966862634687 12-13 26.07277870391032 25.546447370148194 27.79717916070514 20.583594765236338 14-15 22.967504485738175 26.12245179226077 27.59847950867549 23.311564213325564 16-17 20.506091022092015 29.851324195159474 28.154192573691457 21.488392209057057 18-19 20.36692485092386 27.276438291074857 32.22062743893555 20.136009419065733 20-21 21.667657663688423 25.887893954130618 32.614663605732815 19.829784776448136 22-23 21.179793752998442 26.03505792124076 32.55711235821585 20.22803596754494 24-25 20.80131179473313 26.27632219694772 32.809733322657664 20.112632685661485 26-27 20.56305573886258 26.411142325262098 32.8564579657394 20.169343970135923 28-29 20.396442348963248 26.719693220852896 32.80093482846384 20.08292960172002 30-31 20.60187646270504 26.385066003026807 32.79281336835294 20.220244165915215 32-33 20.51862614998313 26.467988171039526 32.613482284779735 20.39990339419761 34-35 20.934779270179916 26.367313374259542 32.598486071569674 20.09942128399087 36-37 20.934549568883483 26.28806642698977 32.47405359784422 20.303330406282527 38-39 20.622516764913193 26.514289389506455 32.60865855755461 20.25453528802574 40-41 20.841203378595665 26.51350978860807 32.44902245177888 20.196264381017386 42-43 20.623493250352297 26.614425021380832 32.11899622003976 20.643085508227106 44-45 20.838075024381155 26.78934028475085 31.77051134133744 20.602073349530556 46-47 21.17507964072092 27.183081121309847 31.00170110217245 20.640138135796782 48-49 21.10364253752991 27.31821111255495 30.734164720768543 20.8439816291466 50-51 21.278543667529032 27.586600670202756 30.384198388415705 20.75065727385251 52-53 21.410038278092888 27.99606882509389 29.81212068510075 20.781772211712475 54-55 21.237370505639177 27.75026825882793 29.912647706428043 21.099713529104854 56-57 21.40451842138768 27.957469820531095 29.705266985098294 20.93274477298293 58-59 21.558746434707736 27.586928814911943 29.95370534442725 20.90061940595307 60-61 21.29970900127189 27.679629695258573 30.36582228470098 20.654839018768563 62-63 21.241003912797513 27.612360029874296 30.41146721374953 20.735168843578666 64-65 21.266179590376826 27.632845327299844 30.2835991579804 20.817375924342933 66-67 21.181150317840967 27.828115176167767 29.89083281814614 21.099901687845126 68-69 21.1510594480081 27.78978787413419 29.962598066046343 21.09655461181137 70-71 21.35240904156806 27.7778105922487 29.800461765234775 21.06931860094847 72-73 21.305287461328145 27.67733268229423 29.821889614744983 21.19549024163264 74-75 21.129073752492257 28.26579458928752 29.28629182040246 21.31883983781776 76-77 21.187056922606416 27.86371887711506 29.71429096460106 21.234933235677467 78-79 21.28838800880478 27.872775671088746 29.583754999284643 21.25508132082183 80-81 21.206679976216073 28.291455505546303 29.269031408698986 21.232833109538642 82-83 21.305586510783574 28.18447259510693 29.34043706594317 21.169503828166324 84-85 21.211970066629803 28.08703056833042 29.266711184980153 21.434288180059625 86-87 21.100262647025236 28.069760940016458 29.36409493095179 21.465881482006512 88-89 21.215605512306084 27.948117696319137 29.29295315799905 21.54332363337573 90-91 21.22821918293753 27.970353081099102 29.249913523256172 21.55151421270719 92-93 21.285425091250488 27.888078320367555 29.332794291229092 21.49370229715287 94-95 21.08063959341558 27.853054174066326 29.385325893636487 21.680980338881604 96-97 21.02242672200499 27.859452995895566 29.33164141921274 21.786478862886703 98-99 20.990794426580358 27.831362741858367 29.10008653298081 22.077756298580457 100-101 20.958323008580894 27.7397735720252 29.471476606435754 21.830426812958155 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 937.0 1 665.0 2 553.5 3 752.5 4 594.0 5 505.0 6 1074.5 7 2638.5 8 4292.5 9 4994.0 10 4439.0 11 3505.0 12 3476.5 13 3799.5 14 3958.0 15 4037.0 16 4141.5 17 4320.0 18 4500.0 19 4667.5 20 4894.5 21 5449.0 22 6220.0 23 7198.5 24 8405.5 25 9825.5 26 12144.5 27 14808.0 28 16830.5 29 19220.5 30 22092.0 31 24351.0 32 27097.0 33 30559.0 34 34260.0 35 37400.0 36 41804.0 37 45904.0 38 47497.5 39 48775.0 40 49395.0 41 50496.0 42 51139.0 43 52301.5 44 54663.0 45 56013.0 46 56396.5 47 58033.0 48 62838.0 49 61155.5 50 53789.5 51 48412.0 52 44338.5 53 42220.5 54 40310.5 55 36783.0 56 32205.5 57 28177.5 58 24945.0 59 22260.0 60 18750.5 61 14945.0 62 11585.0 63 8331.0 64 5986.0 65 4476.5 66 3257.5 67 2408.5 68 1716.0 69 1125.5 70 781.0 71 574.5 72 447.5 73 376.5 74 312.5 75 248.0 76 162.0 77 81.0 78 52.5 79 38.5 80 27.5 81 15.0 82 9.0 83 6.5 84 4.5 85 4.0 86 1.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018310474772891046 2 0.0 3 0.0 4 0.0 5 0.0376710126151952 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0038064786266225115 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.219183602215108E-4 24-25 2.625157673532767E-4 26-27 9.516196566556279E-4 28-29 0.0010500630694131067 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.250315347065534E-4 42-43 0.010303743868616108 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.2814470919159585E-5 54-55 1.968868255149575E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.2814470919159585E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.8754730205983E-4 84-85 3.2814470919159585E-5 86-87 0.0 88-89 0.0 90-91 0.012272612123765682 92-93 0.001804795900553777 94-95 0.0 96-97 0.0 98-99 0.001411022249523862 100-101 0.018080773476456927 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1523718.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.7016444550203 #Duplication Level Percentage of deduplicated Percentage of total 1 74.66162678816647 39.34790509423369 2 14.128629804991895 14.892040492385714 3 4.79712220918824 7.584486872277604 4 2.1683911973386225 4.571111276861434 5 1.1524054487145128 3.036683111309019 6 0.679419324172882 2.1483909395057648 7 0.45442635994101716 1.6764311516820396 8 0.31800236594532744 1.3407398100724723 9 0.23435209385621134 1.111566665492964 >10 1.260712628364777 12.34024498656159 >50 0.09453271548781576 3.431664956922048 >100 0.04662884133419712 4.399395591569483 >500 0.0026251557487007937 1.014369206425876 >1k 7.500444996287984E-4 0.7557205408138743 >5k 1.250074166047997E-4 0.5812018665382268 >10k+ 2.500148332095994E-4 1.7680474373482649 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14324 0.9400689628920837 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12513 0.8212149492228877 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8822 0.5789785248976517 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3000 0.1968868255149575 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2762 0.18126713735743752 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2391 0.15691879993542113 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.2814470919159585E-5 0.0 12-13 0.0 0.0 0.0 6.562894183831917E-5 0.0 14-15 0.0 0.0 0.0 6.562894183831917E-5 0.0 16-17 0.0 0.0 0.0 1.3125788367663834E-4 0.0 18-19 0.0 0.0 0.0 1.3125788367663834E-4 0.0 20-21 0.0 0.0 0.0 1.3125788367663834E-4 0.0 22-23 0.0 0.0 0.0 2.625157673532766E-4 0.0 24-25 0.0 0.0 0.0 4.5940259286823414E-4 0.0 26-27 0.0 0.0 0.0 5.578460056257129E-4 0.0 28-29 0.0 0.0 0.0 0.0014110222495238621 0.0 30-31 0.0 0.0 0.0 0.0025595287316944474 0.0 32-33 0.0 0.0 0.0 0.00472528381235898 0.0 34-35 0.0 0.0 0.0 0.006956667834861831 0.0 36-37 0.0 0.0 0.0 0.010041228101262832 0.0 38-39 0.0 0.0 0.0 0.015816574983034917 0.0 40-41 0.0 0.0 0.0 0.02185443763216028 0.0 42-43 0.0 0.0 0.0 0.02667816485727674 0.0 44-45 0.0 0.0 0.0 0.029631467240001104 0.0 46-47 0.0 0.0 0.0 0.0328800998609979 0.0 48-49 0.0 0.0 0.0 0.03858981780093167 0.0 50-51 0.0 0.0 0.0 0.04515271198476359 0.0 52-53 0.0 0.0 0.0 0.05640807551003532 0.0 54-55 0.0 0.0 0.0 0.06615397337302573 0.0 56-57 0.0 0.0 0.0 0.0885006280689734 0.0 58-59 0.0 0.0 0.0 0.12400588560350406 0.0 60-61 0.0 0.0 0.0 0.16036431938193288 0.0 62-63 0.0 0.0 0.0 0.18297348984523382 0.0 64-65 0.0 0.0 0.0 0.2189053355017136 0.0 66-67 0.0 0.0 0.0 0.27984180799859293 0.0 68-69 0.0 0.0 0.0 0.3636171522552073 0.0 70-71 0.0 0.0 0.0 0.44968950947616293 0.0 72-73 0.0 0.0 0.0 0.5443264436070192 0.0 74-75 0.0 0.0 0.0 0.6527126410530032 0.0 76-77 0.0 0.0 0.0 0.735733252478477 0.0 78-79 0.0 0.0 0.0 0.8419208803728774 0.0 80-81 0.0 0.0 0.0 0.979413513524156 0.0 82-83 0.0 0.0 0.0 1.1095885196604622 0.0 84-85 0.0 0.0 0.0 1.263389944858563 0.0 86-87 0.0 0.0 0.0 1.4632300727562448 0.0 88-89 0.0 0.0 0.0 1.7160655711883694 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGGTC 65 1.3285878E-4 36.53753 8 GGTATCA 10250 0.0 33.147438 1 GTATCAA 14825 0.0 30.194237 1 GGACCGA 335 0.0 26.938726 6 TATCAAC 18225 0.0 24.350304 2 GTACCTG 1135 0.0 24.282866 1 ATCAACG 18330 0.0 24.184896 3 TCAACGC 18455 0.0 24.046831 4 AGGACCG 435 0.0 24.03026 5 CAACGCA 18715 0.0 23.661983 5 AACGCAG 19150 0.0 23.190554 6 GTACATG 9985 0.0 22.938593 1 GAGTACT 11180 0.0 22.920925 12-13 TACATGG 9895 0.0 22.56873 2 AGTACTT 11520 0.0 22.079514 12-13 GTACTTT 11615 0.0 21.75508 14-15 TACCTGG 1265 0.0 21.409641 2 ACATGGG 9940 0.0 20.93692 3 ACGCAGA 21435 0.0 20.497494 7 CGCAGAG 21365 0.0 20.475721 8 >>END_MODULE