FastQCFastQC Report
Thu 26 May 2016
SRR937630_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937630_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571199
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109060.6941195863795738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87010.5537809023554623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59730.3801555372680354No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23940.15236771408332106No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21510.1369018182929088No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20980.133528598223395No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15740.10017827149839073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85350.031.198271
GTATCAA126800.031.0871751
ATCAACG156400.024.8940773
TCAACGC157300.024.8160534
TATCAAC157600.024.704532
CAACGCA159850.024.4186155
GTACATG105600.024.3150881
AACGCAG162400.024.049116
TACATGG105050.023.848632
GAGTACT82900.022.95048912-13
AGTACTT86650.022.56031612-13
ACATGGG106300.022.0476633
ACGCAGA181050.021.519327
GTACTTT87750.021.51924314-15
CGCAGAG181100.021.4609078
CCTATAC4900.021.3439853
AGAGTAC150750.020.92247810-11
GCAGAGT184000.020.6578649
TACCTGG10650.020.5332222
ATGATCG1404.843814E-420.3627538
Produced by FastQC (version 0.11.5)