##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937630_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1571199 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.538383743879674 31.0 31.0 34.0 30.0 34.0 2 31.637819270506156 33.0 31.0 34.0 30.0 34.0 3 31.66923986076875 33.0 31.0 34.0 30.0 34.0 4 35.276161708351395 37.0 35.0 37.0 33.0 37.0 5 35.27286931827222 37.0 35.0 37.0 33.0 37.0 6 35.37561378284991 37.0 35.0 37.0 33.0 37.0 7 35.30966351175122 37.0 35.0 37.0 33.0 37.0 8 35.30229970869381 37.0 35.0 37.0 33.0 37.0 9 36.87273859008312 39.0 37.0 39.0 33.0 39.0 10-11 36.75808729511666 39.0 37.0 39.0 33.0 39.0 12-13 36.625301123536865 39.0 37.0 39.0 32.0 39.0 14-15 37.67724521209598 40.0 37.0 41.0 32.0 41.0 16-17 37.62302419998994 40.0 37.0 41.0 32.0 41.0 18-19 37.632163398780165 40.0 37.0 41.0 32.0 41.0 20-21 37.50010501534179 40.0 37.0 41.0 32.0 41.0 22-23 37.34506863866385 40.0 37.0 41.0 32.0 41.0 24-25 37.19163199569246 39.0 36.5 41.0 31.0 41.0 26-27 37.28720009368641 39.5 37.0 41.0 32.0 41.0 28-29 37.22302299072237 39.5 37.0 41.0 31.0 41.0 30-31 36.99232146914554 39.0 36.0 41.0 31.0 41.0 32-33 36.72156518684139 39.0 35.5 41.0 30.5 41.0 34-35 36.44481189206459 39.0 35.0 40.0 30.0 41.0 36-37 36.41586775449832 39.0 35.0 41.0 30.0 41.0 38-39 36.34775766787021 39.0 35.0 41.0 30.0 41.0 40-41 36.14187604498221 39.0 35.0 41.0 30.0 41.0 42-43 35.85078083680043 39.0 35.0 40.5 28.5 41.0 44-45 35.55229700375318 39.0 35.0 40.0 27.0 41.0 46-47 35.103366600920694 38.5 35.0 40.0 25.0 41.0 48-49 34.700635629223285 38.0 34.0 40.0 24.0 41.0 50-51 33.779940669514176 37.5 33.5 39.5 22.5 40.5 52-53 33.88060996729249 38.0 33.5 39.5 23.0 40.5 54-55 34.177102645813804 38.0 34.0 40.0 22.0 41.0 56-57 33.90143387311219 38.0 33.5 40.0 20.0 41.0 58-59 33.67088987454804 38.0 33.0 40.0 19.0 41.0 60-61 33.35167728594532 37.5 33.0 40.0 18.0 41.0 62-63 32.984540468775755 37.0 32.5 40.0 15.0 41.0 64-65 32.60887386002665 36.5 32.0 39.5 12.0 41.0 66-67 32.183765073679396 36.0 31.0 39.0 8.0 40.5 68-69 31.689230963105246 35.5 31.0 39.0 7.0 40.0 70-71 31.232875975608437 35.0 30.5 38.0 4.0 40.0 72-73 30.746286116526296 35.0 30.0 37.5 2.0 40.0 74-75 30.25039571690155 35.0 29.5 37.0 2.0 39.0 76-77 29.773499728551254 34.0 29.0 36.5 2.0 39.0 78-79 29.218651488449268 34.0 29.0 36.0 2.0 38.5 80-81 28.683036012624754 34.0 28.0 35.5 2.0 37.0 82-83 28.14691741784459 34.0 26.5 35.0 2.0 37.0 84-85 27.645997101576565 33.5 26.0 35.0 2.0 36.5 86-87 27.100978615694128 33.0 25.5 35.0 2.0 36.0 88-89 26.58703480590301 33.0 25.0 35.0 2.0 35.5 90-91 26.037622223537568 32.0 23.5 35.0 2.0 35.0 92-93 25.446084168841757 32.0 20.5 34.5 2.0 35.0 94-95 24.776750430722018 31.5 18.0 34.0 2.0 35.0 96-97 23.963210261717325 31.0 4.5 34.0 2.0 35.0 98-99 22.992727846695423 31.0 2.0 34.0 2.0 35.0 100-101 20.479695124551377 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4164980169273207 1101 2 0.3855698008819637 1101 3 0.45868806086519953 1101 4 0.2829578265221855 1101 5 0.26263950018007876 1101 6 0.3771248978509476 1101 7 0.38840400126332497 1101 8 0.3805493708102219 1101 9 0.46025057849827533 1101 10-11 0.38936282316917215 1101 12-13 0.4767848263208734 1101 14-15 0.5572916508262225 1101 16-17 0.6367536927801396 1101 18-19 0.6546535510022338 1101 20-21 0.6353753872947152 1101 22-23 0.6555958964901549 1101 24-25 0.702107838996163 1101 26-27 0.7924605928906558 1101 28-29 0.7300316667789346 1101 30-31 0.7713291260533381 1101 32-33 0.6192203493907869 1101 34-35 0.7212491268651462 1101 36-37 0.6975611774032586 1101 38-39 0.7097012108826846 1101 40-41 0.8491331379110036 1101 42-43 0.7874970654844375 1101 44-45 0.9639443004029502 1101 46-47 0.9735580718761199 1101 48-49 1.0353630791121375 1101 50-51 1.0130040346721074 1101 52-53 1.0539347187166896 1101 54-55 1.1248403637560713 1101 56-57 1.153592831155514 1101 58-59 1.0764922756772037 1101 60-61 1.251597653835617 1101 62-63 1.2278761461844425 1101 64-65 1.1235396760196465 1101 66-67 1.32930253053933 1101 68-69 1.5630064685978375 1101 70-71 1.5297882316243943 1101 72-73 1.4807538920819923 1101 74-75 1.625053066412665 1101 76-77 1.6409801523150271 1101 78-79 1.6688956423740997 1101 80-81 1.7675309425531402 1101 82-83 1.9905108223864438 1101 84-85 2.117887488654951 1101 86-87 2.4226917337627754 1101 88-89 2.43355737748389 1101 90-91 2.4489351360330716 1101 92-93 2.6087997049656586 1101 94-95 2.788894111996836 1101 96-97 2.951078139358412 1101 98-99 3.2145398599843524 1101 100-101 3.4442585634695213 1102 1 0.2615715369152056 1102 2 0.3179293730445707 1102 3 0.38020459994963574 1102 4 0.1878435268262848 1102 5 0.33950779337452275 1102 6 0.31136907509121414 1102 7 0.28286320407828924 1102 8 0.1416909696078008 1102 9 0.19863855664034702 1102 10-11 0.3342883710736757 1102 12-13 0.3635104487939884 1102 14-15 0.5045642165689372 1102 16-17 0.5585738950474948 1102 18-19 0.5188622207681632 1102 20-21 0.5525678209111788 1102 22-23 0.615853886400096 1102 24-25 0.7605990480562497 1102 26-27 0.7250204225479848 1102 28-29 0.6872056912700586 1102 30-31 0.5362355657518023 1102 32-33 0.5357588512608302 1102 34-35 0.5588293755048568 1102 36-37 0.6777240340800645 1102 38-39 0.5846203796757194 1102 40-41 0.6347032382290863 1102 42-43 0.7722527367766929 1102 44-45 0.5746655769243247 1102 46-47 0.5391711656577982 1102 48-49 0.6681789470790918 1102 50-51 0.43582698083123717 1102 52-53 0.5139283687335805 1102 54-55 0.4942193179313321 1102 56-57 0.6490407962480873 1102 58-59 0.7126236795477752 1102 60-61 0.6116853884645792 1102 62-63 0.6861381150287471 1102 64-65 0.6581893200710596 1102 66-67 0.5975711115220079 1102 68-69 0.7199056669852872 1102 70-71 0.7566603115060104 1102 72-73 0.8028888263101486 1102 74-75 0.6786849363370457 1102 76-77 0.8421027594200154 1102 78-79 0.7917423281489704 1102 80-81 0.9645974251285523 1102 82-83 1.0918615045727016 1102 84-85 1.0992446082093998 1102 86-87 1.3306271736593125 1102 88-89 1.352864530244677 1102 90-91 1.3719430472964298 1102 92-93 1.662181799178434 1102 94-95 1.563347372625774 1102 96-97 1.6758988542849487 1102 98-99 1.9868412628063048 1102 100-101 2.274081929077081 1103 1 0.20669753460789764 1103 2 0.26796834047105733 1103 3 0.1566274082805279 1103 4 0.2049715642060832 1103 5 0.2523251835153104 1103 6 0.23818219056860102 1103 7 0.15153031918680426 1103 8 0.11675724135632493 1103 9 0.3221521889108061 1103 10-11 0.34519066138191334 1103 12-13 0.28774185925207973 1103 14-15 0.43354336944782546 1103 16-17 0.39539101576152547 1103 18-19 0.27009882628037474 1103 20-21 0.3111338738634757 1103 22-23 0.29468747650254556 1103 24-25 0.27045617954735945 1103 26-27 0.12008525675107506 1103 28-29 0.36324759526733885 1103 30-31 0.06891983376090138 1103 32-33 0.3289105294821084 1103 34-35 0.26751938284627386 1103 36-37 0.1981089255679862 1103 38-39 0.31365459325700584 1103 40-41 0.22287495167474702 1103 42-43 0.3526852119225268 1103 44-45 0.10922950339432447 1103 46-47 0.1816866170108966 1103 48-49 0.22388134624296185 1103 50-51 -0.02559210253916433 1103 52-53 0.10584402911845103 1103 54-55 0.15727110030685054 1103 56-57 0.3305225439416901 1103 58-59 0.2847640490783263 1103 60-61 0.4255793102693417 1103 62-63 0.39621051668797946 1103 64-65 0.6074136784194906 1103 66-67 0.557279832269316 1103 68-69 0.7226177908275737 1103 70-71 0.7917517900260123 1103 72-73 0.6683196559637281 1103 74-75 0.6162785124076144 1103 76-77 0.5531461823332258 1103 78-79 0.45927084398728724 1103 80-81 0.7156837212520593 1103 82-83 0.5892400184219575 1103 84-85 0.5530937491177887 1103 86-87 0.617097882088931 1103 88-89 0.7121347092236014 1103 90-91 0.6011637349313652 1103 92-93 0.6587520327660066 1103 94-95 0.6784775835019339 1103 96-97 0.7572980561307929 1103 98-99 0.9389703218378891 1103 100-101 1.2810354900308134 1104 1 0.19073915795727459 1104 2 0.3526945421598917 1104 3 0.4677999011505136 1104 4 0.3319855706075785 1104 5 0.39417032623230597 1104 6 0.45904776143436976 1104 7 0.47674047685514154 1104 8 0.45695661318403324 1104 9 0.4981747301948829 1104 10-11 0.5490214731754861 1104 12-13 0.5015185855259858 1104 14-15 0.5745492465090578 1104 16-17 0.6430545239536087 1104 18-19 0.6026679699255837 1104 20-21 0.6259252045046395 1104 22-23 0.66038163418321 1104 24-25 0.6462089757418639 1104 26-27 0.6290177557504322 1104 28-29 0.7313864554248042 1104 30-31 0.7983220485239642 1104 32-33 0.6606811378352191 1104 34-35 0.801420695596903 1104 36-37 0.6469665599572707 1104 38-39 0.7710596595858519 1104 40-41 0.7967602717031212 1104 42-43 0.8016734036396898 1104 44-45 0.7256164075036011 1104 46-47 0.8346198857040363 1104 48-49 0.8206751841132274 1104 50-51 0.8248508456698715 1104 52-53 0.9079558874666063 1104 54-55 0.8921536405429435 1104 56-57 0.9275623051428639 1104 58-59 1.087259653501512 1104 60-61 1.0264651186217506 1104 62-63 0.9300737410444739 1104 64-65 0.8891931593869629 1104 66-67 0.9689301693626078 1104 68-69 1.061270069328149 1104 70-71 1.0968296995059674 1104 72-73 1.0862578805513934 1104 74-75 0.9764221122584296 1104 76-77 1.0932752777712906 1104 78-79 1.0295120073697674 1104 80-81 1.1909357321211225 1104 82-83 1.2522006623227746 1104 84-85 1.2195129513046332 1104 86-87 1.218432164769986 1104 88-89 1.3241691886873532 1104 90-91 1.396691364868932 1104 92-93 1.4145336528481423 1104 94-95 1.535141489310437 1104 96-97 1.4426529144705889 1104 98-99 1.8419963897109675 1104 100-101 1.5870979734909874 1105 1 0.2848580799661633 1105 2 0.37793715664971117 1105 3 0.3629246419350025 1105 4 0.37977189283657964 1105 5 0.46210799620924803 1105 6 0.385746386680097 1105 7 0.4674188648336681 1105 8 0.4318456842739593 1105 9 0.41893541518440713 1105 10-11 0.4699691085126432 1105 12-13 0.44679502378077984 1105 14-15 0.6256300828895078 1105 16-17 0.6858560072945181 1105 18-19 0.6889386472600734 1105 20-21 0.5827966659943797 1105 22-23 0.6890621058161983 1105 24-25 0.6120611778917464 1105 26-27 0.6792378865465949 1105 28-29 0.5932620984713992 1105 30-31 0.6928859382655688 1105 32-33 0.7484808790406774 1105 34-35 0.7439462005031672 1105 36-37 0.7141706764916691 1105 38-39 0.7159587430973957 1105 40-41 0.8776860256462342 1105 42-43 0.8797068241445558 1105 44-45 0.7702543649868616 1105 46-47 0.9196196892550645 1105 48-49 0.8312439233382136 1105 50-51 0.7766083732277735 1105 52-53 0.5654300371555507 1105 54-55 0.5246580455335206 1105 56-57 0.623958394642429 1105 58-59 0.8363111935103902 1105 60-61 0.9065217909141978 1105 62-63 0.8211056508351078 1105 64-65 0.938332173161271 1105 66-67 0.9698111674788592 1105 68-69 1.0515575022494552 1105 70-71 0.9890292933962783 1105 72-73 0.9121682488694844 1105 74-75 0.9638713971193802 1105 76-77 0.9291665495215398 1105 78-79 0.8574559464438138 1105 80-81 1.0982994602132834 1105 82-83 1.0748098242714264 1105 84-85 1.1964388313205028 1105 86-87 1.057220951030473 1105 88-89 0.98795928100974 1105 90-91 1.1872003317934023 1105 92-93 1.3933242870243063 1105 94-95 1.3829734049729474 1105 96-97 1.5068397042049853 1105 98-99 1.80971615243514 1105 100-101 1.6408377791921502 1106 1 0.14424737597911275 1106 2 0.3169332618349081 1106 3 0.2042455331201829 1106 4 0.1914353534098865 1106 5 0.14191819988877086 1106 6 0.21959075353208135 1106 7 0.12579416239727692 1106 8 0.16265321912344177 1106 9 0.40953977858314516 1106 10-11 0.07120413945759907 1106 12-13 0.06794673072084834 1106 14-15 0.14456262522584495 1106 16-17 0.05434270507068817 1106 18-19 0.06600228156963084 1106 20-21 0.11079315161487102 1106 22-23 0.04004877984097277 1106 24-25 0.07142792041140211 1106 26-27 0.1581555336637379 1106 28-29 0.3437725468108894 1106 30-31 0.3709971947305988 1106 32-33 0.3337582808121553 1106 34-35 0.08187167566519804 1106 36-37 0.2662198844138075 1106 38-39 0.1837382277624613 1106 40-41 0.1914476633107398 1106 42-43 0.31403505843321966 1106 44-45 0.2949574622919684 1106 46-47 0.4950600916490302 1106 48-49 0.5255309086920761 1106 50-51 0.500748996323793 1106 52-53 0.4218491702835365 1106 54-55 0.3741944390647163 1106 56-57 0.5201274400968074 1106 58-59 0.24616971371208507 1106 60-61 0.17686175797619086 1106 62-63 0.4261448282356781 1106 64-65 0.27793614868949135 1106 66-67 0.615473069134886 1106 68-69 0.5181570591930971 1106 70-71 0.37355272279950213 1106 72-73 0.4948895880678279 1106 74-75 0.6861583791686989 1106 76-77 0.7691835926924782 1106 78-79 0.7716237948281588 1106 80-81 0.5706943561842657 1106 82-83 0.35501095516801584 1106 84-85 0.5017185380094311 1106 86-87 0.40064931332879894 1106 88-89 0.6994620995767988 1106 90-91 1.1101801521998524 1106 92-93 1.0129542527686084 1106 94-95 1.0169601562413746 1106 96-97 0.9422677310923824 1106 98-99 1.00684979344879 1106 100-101 0.8099410630386039 1107 1 0.34234385512179344 1107 2 0.44437282082268936 1107 3 0.35513574159899264 1107 4 0.4119590468357117 1107 5 0.4114574052638176 1107 6 0.39703880540213987 1107 7 0.4849495752800337 1107 8 0.3379188576080381 1107 9 0.47462614004364667 1107 10-11 0.4761844428336488 1107 12-13 0.4367709469307641 1107 14-15 0.5805413055177269 1107 16-17 0.4195177699683157 1107 18-19 0.5186237941262348 1107 20-21 0.7719700420532618 1107 22-23 0.6709285995516652 1107 24-25 0.6307513465258978 1107 26-27 0.6389944331234503 1107 28-29 0.6811336367136249 1107 30-31 0.5602752311075889 1107 32-33 0.731671905625781 1107 34-35 0.7864504115468591 1107 36-37 0.6959687353011006 1107 38-39 0.7749041718192444 1107 40-41 0.7483416103734868 1107 42-43 0.6722092860527553 1107 44-45 0.6997447456162362 1107 46-47 0.9596250416096623 1107 48-49 1.0431191102770683 1107 50-51 1.013813665761944 1107 52-53 0.9712417147339067 1107 54-55 0.9111262523722914 1107 56-57 1.1465156832025585 1107 58-59 1.0061314785845 1107 60-61 1.0275879851976768 1107 62-63 0.9049456504153142 1107 64-65 1.0462945002810926 1107 66-67 1.093304287254412 1107 68-69 1.3050258192837596 1107 70-71 1.2420859076646735 1107 72-73 1.2460530700466137 1107 74-75 1.4354317659071079 1107 76-77 1.4638586841839825 1107 78-79 1.372887475660498 1107 80-81 1.374814605102923 1107 82-83 1.382375622375715 1107 84-85 1.4685214482370945 1107 86-87 1.406244321229753 1107 88-89 1.569317257329942 1107 90-91 1.848452039386924 1107 92-93 1.8428300250632823 1107 94-95 1.871560295902107 1107 96-97 2.0405599754609156 1107 98-99 2.4554545587278334 1107 100-101 2.4416084045605615 1108 1 0.2064264945604002 1108 2 0.11273456125429249 1108 3 0.167059374768332 1108 4 0.22650879182313588 1108 5 0.27649613310678234 1108 6 0.22525017692557014 1108 7 0.17829152593418485 1108 8 0.18940868549542245 1108 9 0.24711551034462076 1108 10-11 0.2990053962834196 1108 12-13 0.2749970313604706 1108 14-15 0.34184736402866633 1108 16-17 0.21474200401208066 1108 18-19 0.3037731944208275 1108 20-21 0.2566859877708225 1108 22-23 0.2611336617266389 1108 24-25 0.26124655856877865 1108 26-27 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7 2009.0 8 2492.0 9 3103.0 10 4037.0 11 5038.0 12 6881.0 13 9090.0 14 10660.0 15 13222.0 16 16763.0 17 22187.0 18 18829.0 19 12974.0 20 12428.0 21 12942.0 22 14149.0 23 15415.0 24 17229.0 25 19438.0 26 22483.0 27 25991.0 28 30839.0 29 36630.0 30 43886.0 31 53868.0 32 66910.0 33 86114.0 34 113137.0 35 154818.0 36 210980.0 37 273399.0 38 198365.0 39 22672.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.01412385187834 19.669634676227297 15.118012699973502 21.198228771920864 2 14.786142211902922 20.797148945757087 41.42810552832131 22.988603314018683 3 19.31403219239365 26.286157730220545 30.03904390301183 24.36076617437398 4 11.913593799462866 17.040371909687828 38.367163805691995 32.67887048515731 5 12.597085875963678 39.04931314674719 33.89939768302311 14.454203294266021 6 29.949541304884438 37.31947032083463 17.957899423210744 14.773088951070182 7 27.39950203827571 34.50508676064673 20.54366348807902 17.551747712998537 8 26.334992321546096 35.05271602985668 19.566282148742623 19.046009499854595 9 26.821279912439262 17.056548366974397 20.777114297122257 35.345057423464084 10-11 24.51600229230601 27.876998711933226 26.746185150589646 20.860813845171123 12-13 25.898114894672762 25.40918782363675 27.75346748532932 20.93922979636117 14-15 22.92567095896291 26.137542481550803 27.306185747501495 23.630600811984795 16-17 20.755459970314725 29.96962918913921 27.67662499780788 21.59828584273819 18-19 20.685684694604117 27.78924114427037 31.118017829281612 20.407056331843894 20-21 22.11464056734586 26.486491484423848 31.183202297899935 20.21566565033036 22-23 21.434033136487123 26.73076923076923 31.13930589390712 20.695891738836526 24-25 20.983558675332144 26.943116371110566 31.406463141421153 20.666861812136137 26-27 20.739889723466863 27.070522901576343 31.497176762594243 20.69241061236255 28-29 20.6228537460264 27.360719916726623 31.424060240533517 20.59236609671346 30-31 20.814816137640378 27.08159003821643 31.40645010689375 20.69714371724944 32-33 20.709679682616628 27.12782717403784 31.27128752025105 20.89120562309449 34-35 21.079141852622516 27.19233375586967 31.13384834931796 20.594676042189857 36-37 21.06689393808958 26.959913121608704 31.148428517589622 20.824764422712093 38-39 20.837239996365646 27.31792771783399 31.125485576562888 20.71934670923747 40-41 20.98693801638797 27.281766557330638 31.120089084048047 20.61120634223334 42-43 20.749411488246825 27.23278154743438 30.862025041601555 21.15578192271724 44-45 20.949064257486548 27.425638330911102 30.597922471148443 21.027374940453907 46-47 21.22062973296134 27.67432443204464 29.910466321243522 21.194579513750497 48-49 21.13502386225745 27.856000484739475 29.75751864413266 21.251457008870414 50-51 21.285992860024265 28.067981242814984 29.516640544156083 21.129385353004672 52-53 21.30696923312951 28.434181555825532 29.10006720415103 21.15878200689393 54-55 21.209274681349434 28.157824420300532 29.22787939776685 21.405021500583192 56-57 21.389767430591487 28.472115293575325 28.911189555560448 21.226927720272748 58-59 21.46840062777613 28.113289171371854 29.26545538476154 21.15285481609048 60-61 21.385237807136605 28.16355245349081 29.529772830001512 20.921436909371074 62-63 21.186094604102227 28.207395685462288 29.63173953252321 20.974770177912276 64-65 21.185958995267704 28.229764214022836 29.53287503327271 21.05140175743675 66-67 21.158285157787862 28.293780772179478 29.124105624385354 21.423828445647306 68-69 21.07261274742174 28.379665153602236 29.185651698921962 21.362070400054066 70-71 21.295481019542486 28.27236680780577 29.129466491629525 21.302685681022222 72-73 21.226637491524322 28.200809903481666 29.107464430840857 21.46508817415316 74-75 20.962300220885684 28.812509883741694 28.79481479781053 21.430375097562095 76-77 21.183256362009853 28.45212081872919 28.91892219318219 21.44570062607877 78-79 21.195619771921308 28.433415196127974 28.84109255108082 21.529872480869898 80-81 21.162542033764733 28.805291057918286 28.619526365388026 21.412640542928955 82-83 21.245244830471332 28.720124307741347 28.665970755807002 21.368660105980318 84-85 21.24579824299144 28.576728720606827 28.627152992084476 21.55032004431726 86-87 21.082292520330956 28.643243951395043 28.73192144232568 21.542542085948316 88-89 21.280738367928656 28.46710046696276 28.57946607052862 21.67269509457996 90-91 21.255966572846464 28.45343945249551 28.679175389586565 21.611418585071462 92-93 21.360781283471557 28.382730972855523 28.696471014270998 21.560016729401923 94-95 21.16540655918935 28.423408863276585 28.664145814167142 21.747038763366923 96-97 21.06518680840849 28.423205627762897 28.66242444316433 21.84918312066428 98-99 21.069342802724766 28.44987601074042 28.482108754744846 21.998672431789966 100-101 21.406492706280318 27.927696180387873 28.89956524247637 21.766245870855435 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2454.0 1 1461.5 2 538.0 3 646.0 4 602.0 5 626.0 6 1046.5 7 2105.0 8 3370.5 9 3991.0 10 3670.5 11 3204.5 12 3216.0 13 3296.0 14 3448.0 15 3676.5 16 3772.5 17 3825.0 18 3966.5 19 4247.0 20 4650.0 21 5164.0 22 5987.5 23 7101.5 24 8212.5 25 9829.0 26 12329.0 27 15058.5 28 17203.0 29 19749.0 30 22823.5 31 25209.5 32 28299.5 33 32146.5 34 36178.5 35 39442.0 36 43603.0 37 47515.5 38 48793.5 39 49910.5 40 50831.5 41 52185.0 42 53257.5 43 54390.5 44 56185.5 45 57367.5 46 58029.0 47 59752.0 48 63988.0 49 62541.0 50 55634.0 51 50050.0 52 45744.5 53 43672.0 54 41885.5 55 38306.5 56 33875.0 57 29731.5 58 26451.0 59 23584.5 60 19736.5 61 16017.0 62 12443.0 63 8971.0 64 6788.5 65 5168.0 66 3730.5 67 2719.5 68 1936.5 69 1337.0 70 954.0 71 699.0 72 523.0 73 419.0 74 331.5 75 250.0 76 176.0 77 124.5 78 80.0 79 53.0 80 37.0 81 19.5 82 14.0 83 9.5 84 6.5 85 9.0 86 9.0 87 7.5 88 5.5 89 3.5 90 4.5 91 4.0 92 1.5 93 1.0 94 1.5 95 1.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32166517417590007 2 0.2762221717299973 3 0.270175833869548 4 0.2779406045955986 5 0.2810592420183567 6 0.21524962783199328 7 0.20411163703642887 8 0.2020749758623828 9 0.21467681687679283 10-11 0.21292656118034697 12-13 0.20850318769296566 14-15 0.21082625434461197 16-17 0.19612410649446696 18-19 0.20115211376789316 20-21 0.18995047731063983 22-23 0.19978373204158098 24-25 0.19972008637989205 26-27 0.19991102336495886 28-29 0.21327661231963616 30-31 0.20920328997154403 32-33 0.21505869084692647 34-35 0.20917146714069956 36-37 0.19284635491748658 38-39 0.1802126910722321 40-41 0.17773051026636347 42-43 0.19402379965873195 44-45 0.19641051197206721 46-47 0.1956467640317999 48-49 0.2129902068420359 50-51 0.20789855390692075 52-53 0.22832881130907035 54-55 0.1974288425590902 56-57 0.1772849906345409 58-59 0.17855790386831966 60-61 0.1678972555354223 62-63 0.17550291210725058 64-65 0.17321166828644874 66-67 0.17289343997800405 68-69 0.17493010115205013 70-71 0.1762984828783623 72-73 0.1744209358585386 74-75 0.1888366782310834 76-77 0.1824084664005005 78-79 0.18613173760930346 80-81 0.17928982897774248 82-83 0.15984607933177145 84-85 0.15975061083923806 86-87 0.1650650235902645 88-89 0.16920199160004556 90-91 0.1765212426942736 92-93 0.17203422354520337 94-95 0.17069766464973565 96-97 0.17569384909231742 98-99 0.18571804080832535 100-101 0.16697439344093268 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1571199.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.185571166920056 #Duplication Level Percentage of deduplicated Percentage of total 1 76.22332290293697 42.06427610639156 2 13.269115506321597 14.645274361923857 3 4.556802950676353 7.544093205845435 4 2.04245796027735 4.508568364893123 5 1.05683210850127 2.9160941767591506 6 0.6549768461556645 2.1687162813724967 7 0.4044887574884976 1.5625360178820387 8 0.27663953637035066 1.2213208657559804 9 0.20991603222853392 1.0425902522062718 >10 1.1769136620734082 11.992076091461671 >50 0.0842648255747666 3.2282307335704674 >100 0.04080151321131818 3.893961445562863 >500 0.0023115987894146615 0.8191070617062526 >1k 8.090595762951315E-4 0.761574390030576 >5k 2.3115987894146615E-4 0.9359583416506426 >10k+ 1.1557993947073307E-4 0.6956223029877271 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10906 0.6941195863795738 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8701 0.5537809023554623 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5973 0.3801555372680354 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2394 0.15236771408332106 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2151 0.1369018182929088 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2098 0.133528598223395 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1574 0.10017827149839073 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.1822830844469736E-5 0.0 14-15 0.0 0.0 0.0 6.364566168893947E-5 0.0 16-17 0.0 0.0 0.0 6.364566168893947E-5 0.0 18-19 0.0 0.0 0.0 6.364566168893947E-5 0.0 20-21 0.0 0.0 0.0 1.5911415422234867E-4 0.0 22-23 0.0 0.0 0.0 2.545826467557579E-4 0.0 24-25 0.0 0.0 0.0 3.1822830844469734E-4 0.0 26-27 0.0 0.0 0.0 6.046337860449249E-4 0.0 28-29 0.0 0.0 0.0 0.0014002045571566682 0.0 30-31 0.0 0.0 0.0 0.002736763452624397 0.0 32-33 0.0 0.0 0.0 0.005441704074404324 0.0 34-35 0.0 0.0 0.0 0.0069055542932499325 0.0 36-37 0.0 0.0 0.0 0.009642317745874329 0.0 38-39 0.0 0.0 0.0 0.015434072959567821 0.0 40-41 0.0 0.0 0.0 0.021925930451839645 0.0 42-43 0.0 0.0 0.0 0.026985760556110336 0.0 44-45 0.0 0.0 0.0 0.02978616967042367 0.0 46-47 0.0 0.0 0.0 0.03430501165033837 0.0 48-49 0.0 0.0 0.0 0.04016041252572081 0.0 50-51 0.0 0.0 0.0 0.046843207003059446 0.0 52-53 0.0 0.0 0.0 0.06065431558955932 0.0 54-55 0.0 0.0 0.0 0.06911918859418825 0.0 56-57 0.0 0.0 0.0 0.09359094551358549 0.0 58-59 0.0 0.0 0.0 0.13120553157174872 0.0 60-61 0.0 0.0 0.0 0.16786543270457782 0.0 62-63 0.0 0.0 0.0 0.18972771749472855 0.0 64-65 0.0 0.0 0.0 0.22778782318471436 0.0 66-67 0.0 0.0 0.0 0.2950931104207678 0.0 68-69 0.0 0.0 0.0 0.38378333998430497 0.0 70-71 0.0 0.0 0.0 0.47301455767219813 0.0 72-73 0.0 0.0 0.0 0.567655656603651 0.0 74-75 0.0 0.0 0.0 0.6766488522459599 0.0 76-77 0.0 0.0 0.0 0.7605020115211377 0.0 78-79 0.0 0.0 0.0 0.8668857350342001 0.0 80-81 0.0 0.0 0.0 1.0044240099439983 0.0 82-83 0.0 0.0 0.0 1.1350567305605463 0.0 84-85 0.0 0.0 0.0 1.2859924172558663 0.0 86-87 0.0 0.0 0.0 1.4848532872029576 0.0 88-89 0.0 0.0 0.0 1.7273114354069725 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8535 0.0 31.19827 1 GTATCAA 12680 0.0 31.087175 1 ATCAACG 15640 0.0 24.894077 3 TCAACGC 15730 0.0 24.816053 4 TATCAAC 15760 0.0 24.70453 2 CAACGCA 15985 0.0 24.418615 5 GTACATG 10560 0.0 24.315088 1 AACGCAG 16240 0.0 24.04911 6 TACATGG 10505 0.0 23.84863 2 GAGTACT 8290 0.0 22.950489 12-13 AGTACTT 8665 0.0 22.560316 12-13 ACATGGG 10630 0.0 22.047663 3 ACGCAGA 18105 0.0 21.51932 7 GTACTTT 8775 0.0 21.519243 14-15 CGCAGAG 18110 0.0 21.460907 8 CCTATAC 490 0.0 21.343985 3 AGAGTAC 15075 0.0 20.922478 10-11 GCAGAGT 18400 0.0 20.657864 9 TACCTGG 1065 0.0 20.533222 2 ATGATCG 140 4.843814E-4 20.362753 8 >>END_MODULE