FastQCFastQC Report
Thu 26 May 2016
SRR937630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937630_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571199
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146550.9327271720514079No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125040.795825353758499No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87580.5574087050717319No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29870.19010959146486217No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28660.1824084664005005No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22910.1458122109293603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104250.032.1372571
GTACCGT804.494701E-429.6871416
GTATCAA150200.028.918251
CTCCGTA700.00839298427.1425327
ACGCACT1151.2807667E-424.7862534
GTACATG103450.023.6117611
TACATGG102800.023.1989382
TATCAAC187800.022.9187052
ATCAACG188050.022.8629743
TCAACGC188650.022.7650764
AGTACTT115000.022.61349712-13
ACATGGG99450.022.5950783
CAACGCA190550.022.5380825
TGTACCG1903.283876E-722.503315
GGACCGA3600.022.4302856
GAGTACT109700.022.32047812-13
AACGCAG194350.022.1672136
TACCGTG1302.9424127E-421.9228137
CGCCGTA550.002877071521.5927190-91
AGAGTAC174050.021.05475810-11
Produced by FastQC (version 0.11.5)