##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937630_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1571199 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18097834838235 33.0 31.0 34.0 31.0 34.0 2 32.38042348550375 34.0 31.0 34.0 31.0 34.0 3 32.46095561415199 34.0 31.0 34.0 31.0 34.0 4 36.10124369987506 37.0 35.0 37.0 35.0 37.0 5 36.02668471657632 37.0 35.0 37.0 35.0 37.0 6 36.072671252973045 37.0 35.0 37.0 35.0 37.0 7 36.022321170010926 37.0 35.0 37.0 35.0 37.0 8 36.00860934865666 37.0 35.0 37.0 35.0 37.0 9 37.66591946659844 39.0 37.0 39.0 35.0 39.0 10-11 37.60639231567739 39.0 37.0 39.0 35.0 39.0 12-13 37.510390472499026 39.0 37.0 39.0 35.0 39.0 14-15 38.674578458871224 40.0 38.0 41.0 34.0 41.0 16-17 38.64151262825396 40.0 38.0 41.0 34.5 41.0 18-19 38.701226897420376 40.0 38.0 41.0 34.0 41.0 20-21 38.630599943100776 40.0 38.0 41.0 34.0 41.0 22-23 38.47960411125516 40.0 38.0 41.0 34.0 41.0 24-25 38.548281917185534 40.0 38.0 41.0 34.0 41.0 26-27 38.41923397354505 40.0 38.0 41.0 34.0 41.0 28-29 38.27572669025375 40.0 38.0 41.0 34.0 41.0 30-31 38.2347248820805 40.0 37.5 41.0 34.0 41.0 32-33 38.093341454519766 40.0 37.0 41.0 34.0 41.0 34-35 37.93440964511815 40.0 37.0 41.0 33.0 41.0 36-37 37.755982533084605 40.0 37.0 41.0 33.0 41.0 38-39 37.60178436977111 40.0 37.0 41.0 33.0 41.0 40-41 37.4010274955623 40.0 36.0 41.0 33.0 41.0 42-43 37.13807958126246 40.0 36.0 41.0 32.5 41.0 44-45 36.74588419417273 40.0 36.0 41.0 31.0 41.0 46-47 36.26963961916982 39.0 36.0 41.0 30.0 41.0 48-49 35.83687521440632 39.0 35.0 41.0 28.5 41.0 50-51 35.54188839224058 39.0 35.0 41.0 27.5 41.0 52-53 35.29263797902112 39.0 35.0 40.0 27.0 41.0 54-55 35.075017232062905 39.0 35.0 40.0 26.0 41.0 56-57 34.81721443305399 38.0 34.5 40.0 26.0 41.0 58-59 34.55617875265959 38.0 34.0 40.0 25.0 41.0 60-61 34.21153081181951 38.0 34.0 40.0 24.0 41.0 62-63 33.87236976347363 37.5 34.0 40.0 23.0 41.0 64-65 33.484443409141676 37.0 33.0 39.5 22.0 41.0 66-67 33.04924423959027 36.0 33.0 39.0 21.5 40.5 68-69 32.60889772714978 36.0 32.0 39.0 19.5 40.0 70-71 32.19631440702292 35.0 32.0 38.5 18.5 40.0 72-73 31.7044986026595 35.0 31.0 37.5 17.0 40.0 74-75 31.200286532768924 35.0 31.0 37.0 12.0 39.0 76-77 29.953426014145883 33.5 29.5 35.5 10.5 38.5 78-79 30.54884613597641 34.0 30.5 36.0 8.0 38.5 80-81 30.344612935726154 34.0 30.5 36.0 7.0 37.5 82-83 29.96975239928233 34.0 30.0 35.0 2.0 37.0 84-85 29.562495584582223 34.0 30.0 35.0 2.0 36.5 86-87 29.15008856293824 34.0 29.0 35.0 2.0 36.0 88-89 28.76282762399925 34.0 29.0 35.0 2.0 36.0 90-91 28.222607066323235 34.0 28.5 35.0 2.0 35.0 92-93 27.74392008905301 33.0 27.0 35.0 2.0 35.0 94-95 27.262712743579904 33.0 26.5 35.0 2.0 35.0 96-97 26.700623536547567 33.0 25.5 35.0 2.0 35.0 98-99 25.82523283174187 32.5 24.5 34.0 2.0 35.0 100-101 23.188967151837545 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3822567097213323 1101 2 0.40324214100457567 1101 3 0.26912595033687126 1101 4 0.1536167694276287 1101 5 0.18081062739124576 1101 6 0.26165838020600063 1101 7 0.28110476391694306 1101 8 0.21934973786711254 1101 9 0.40159863161890286 1101 10-11 0.3955240666772184 1101 12-13 0.40105479272835964 1101 14-15 0.4539007701617308 1101 16-17 0.3920837496774965 1101 18-19 0.4955892911140225 1101 20-21 0.39937704582174405 1101 22-23 0.45216741442841624 1101 24-25 0.4307315829353371 1101 26-27 0.31219497904567817 1101 28-29 0.4529183341524501 1101 30-31 0.3966463132667286 1101 32-33 0.47168523137820273 1101 34-35 0.5263555296479012 1101 36-37 0.4183538585513702 1101 38-39 0.3239067859856064 1101 40-41 0.42754450475751327 1101 42-43 0.5191526155134412 1101 44-45 0.6987832629893092 1101 46-47 0.7030027733155109 1101 48-49 0.5854964130709774 1101 50-51 0.5049288932865892 1101 52-53 0.3417777828527164 1101 54-55 0.45360410682570773 1101 56-57 0.5021741000985429 1101 58-59 0.43476921435744487 1101 60-61 0.22558509312940345 1101 62-63 0.429704191001143 1101 64-65 0.5320359679226101 1101 66-67 0.4127288610801827 1101 68-69 0.379992469682378 1101 70-71 0.4374254483913589 1101 72-73 0.45361000099616433 1101 74-75 0.4342682693610236 1101 76-77 0.31672530108103913 1101 78-79 0.39112973833429976 1101 80-81 0.2214309057132695 1101 82-83 0.3405891010427382 1101 84-85 0.5073862424063584 1101 86-87 0.34027078314933235 1101 88-89 0.472126595946186 1101 90-91 0.5893438130432038 1101 92-93 0.7223043690184276 1101 94-95 0.6138692851229699 1101 96-97 0.7260140476927575 1101 98-99 0.8878238903600391 1101 100-101 1.355433901189567 1102 1 0.299575851716277 1102 2 0.29026341636081554 1102 3 0.2605986221068264 1102 4 0.12854453108771224 1102 5 0.09016316214137987 1102 6 0.18968802122805783 1102 7 0.1790972371479711 1102 8 0.18660654516551034 1102 9 0.37281980910384505 1102 10-11 0.3548637849476961 1102 12-13 0.3443447830577355 1102 14-15 0.4015811329846102 1102 16-17 0.346587431502563 1102 18-19 0.4136417396442056 1102 20-21 0.48472387723843013 1102 22-23 0.5313084481357606 1102 24-25 0.4135072537615301 1102 26-27 0.43348184098888964 1102 28-29 0.4595850882042072 1102 30-31 0.40006088560411257 1102 32-33 0.33741076064055164 1102 34-35 0.3581465161673023 1102 36-37 0.3436512591362657 1102 38-39 0.37944510725980507 1102 40-41 0.32471628636469774 1102 42-43 0.4112932006439962 1102 44-45 0.36177028921648 1102 46-47 0.4077125383078837 1102 48-49 0.24913737104457567 1102 50-51 0.057760461441134225 1102 52-53 0.09627593028884007 1102 54-55 0.12317851231581756 1102 56-57 -0.16513994443289448 1102 58-59 -0.11054505154955763 1102 60-61 -0.13379045302740877 1102 62-63 -0.1238439348799929 1102 64-65 0.022722173895090236 1102 66-67 0.022242766960616223 1102 68-69 -0.01840343137439504 1102 70-71 -0.0789279427326477 1102 72-73 0.05973700648394953 1102 74-75 -0.009823479156342074 1102 76-77 -0.07008260901718089 1102 78-79 0.04064853777969546 1102 80-81 -0.10177012659591256 1102 82-83 -0.016670997760670048 1102 84-85 0.0520400226852864 1102 86-87 0.28286533381520584 1102 88-89 0.29244173570407384 1102 90-91 0.35238094005451615 1102 92-93 0.46802978176733134 1102 94-95 0.3769413661697243 1102 96-97 0.5427682450898459 1102 98-99 0.7025594922102734 1102 100-101 1.1116156679995441 1103 1 0.22377380985929562 1103 2 0.25962064368828663 1103 3 0.28058185978461836 1103 4 0.17887715559361084 1103 5 0.16799675405206926 1103 6 0.1873156192071761 1103 7 0.20457524360698187 1103 8 0.23495056515734092 1103 9 0.21791931617946148 1103 10-11 0.24829646261564875 1103 12-13 0.28601027437279924 1103 14-15 0.24684631943154756 1103 16-17 0.42527789994346676 1103 18-19 0.2855679691524031 1103 20-21 0.31059033740486797 1103 22-23 0.4654930524762406 1103 24-25 0.397983345301526 1103 26-27 0.27154167000235674 1103 28-29 0.3040225558820353 1103 30-31 0.44919532955429986 1103 32-33 0.4760846204590763 1103 34-35 0.34778398348348105 1103 36-37 0.27904308519869403 1103 38-39 0.27721460634302986 1103 40-41 0.27086642216301726 1103 42-43 0.3736611544110886 1103 44-45 0.5076962945037593 1103 46-47 0.3146378004112975 1103 48-49 0.2424543669905148 1103 50-51 0.32683054132887435 1103 52-53 0.37963896385326734 1103 54-55 0.45794328829379793 1103 56-57 0.516727409000751 1103 58-59 0.3277311325719907 1103 60-61 0.48218194248384094 1103 62-63 0.5567140316532289 1103 64-65 0.5103054882419755 1103 66-67 0.5182782155652035 1103 68-69 0.5140774103943428 1103 70-71 0.5345447963429066 1103 72-73 0.5062180489665806 1103 74-75 0.4623647633494343 1103 76-77 0.30449539454865615 1103 78-79 0.5804215321777875 1103 80-81 0.42150409559255664 1103 82-83 0.6045482125477051 1103 84-85 0.5822501755533267 1103 86-87 0.7530179577424718 1103 88-89 0.7527923792542843 1103 90-91 0.8041949144427001 1103 92-93 0.7161352053821339 1103 94-95 0.7572831132123596 1103 96-97 0.6853557997436965 1103 98-99 0.7709254008655613 1103 100-101 1.0938807423360153 1104 1 0.2877988592531153 1104 2 0.28828513249139576 1104 3 0.1281626743302482 1104 4 0.15044471978136187 1104 5 0.16170314229037075 1104 6 0.13816265665665384 1104 7 0.2523605465946517 1104 8 0.2024028214653697 1104 9 0.23263024288110046 1104 10-11 0.26585873493841916 1104 12-13 0.3036093141049392 1104 14-15 0.36821003365322014 1104 16-17 0.3255182311055407 1104 18-19 0.2992073570432936 1104 20-21 0.27975062701756315 1104 22-23 0.23304772913660088 1104 24-25 0.1030387099747827 1104 26-27 0.25665857495265243 1104 28-29 0.12591704178338858 1104 30-31 0.34875843585879096 1104 32-33 0.35411369357250067 1104 34-35 0.2491991104323219 1104 36-37 0.2852107630791991 1104 38-39 0.28659051589608 1104 40-41 0.2931012377733637 1104 42-43 0.3706777103131813 1104 44-45 0.23697411653452605 1104 46-47 0.37932532366613003 1104 48-49 0.1582719706977258 1104 50-51 0.09445346886889894 1104 52-53 0.18811070336563773 1104 54-55 0.06776952155417604 1104 56-57 0.17901941444497993 1104 58-59 0.23904197657997628 1104 60-61 0.0804709149470284 1104 62-63 0.2853745134692147 1104 64-65 0.16052171143203964 1104 66-67 -0.0024755559563871543 1104 68-69 0.1446543328785168 1104 70-71 0.04364726204904201 1104 72-73 0.13623905702766592 1104 74-75 -0.050457375298485374 1104 76-77 8.920582308391545E-5 1104 78-79 0.104970713101185 1104 80-81 0.1601036668009037 1104 82-83 0.31674482087528233 1104 84-85 0.34731831855257056 1104 86-87 0.11070078313514742 1104 88-89 0.16796732387199853 1104 90-91 0.2862924885422409 1104 92-93 0.46776937362187354 1104 94-95 0.4926696091373408 1104 96-97 0.9141395734713349 1104 98-99 0.9305536405256625 1104 100-101 1.3114202937207828 1105 1 0.2989774538268932 1105 2 0.3140916203160913 1105 3 0.31161536660954425 1105 4 0.1571395283040502 1105 5 0.18241749986118805 1105 6 0.18993624553772293 1105 7 0.20079204181317323 1105 8 0.23183390090286338 1105 9 0.23176457210281853 1105 10-11 0.27564211000097316 1105 12-13 0.32839016555367095 1105 14-15 0.2758623906308131 1105 16-17 0.35285443043519393 1105 18-19 0.3102272812873821 1105 20-21 0.33123947281720234 1105 22-23 0.24120359996038587 1105 24-25 0.19657627253083376 1105 26-27 0.14946824006974424 1105 28-29 0.23099997563448227 1105 30-31 0.27249996426639456 1105 32-33 0.43257951771050784 1105 34-35 0.43134919486220014 1105 36-37 0.43283116147772915 1105 38-39 0.3121951577911801 1105 40-41 0.42899687025920485 1105 42-43 0.5296899907490626 1105 44-45 0.37960276794898107 1105 46-47 0.35213289908418943 1105 48-49 0.488543290485147 1105 50-51 0.36981254953948195 1105 52-53 0.4884975999164709 1105 54-55 0.5930775470778613 1105 56-57 0.6681918374984974 1105 58-59 0.7215495981527553 1105 60-61 0.7293550631550474 1105 62-63 0.6187434666724769 1105 64-65 0.6383676634820787 1105 66-67 0.6346626702949862 1105 68-69 0.6206061713603219 1105 70-71 0.5330498110501125 1105 72-73 0.5900396539735127 1105 74-75 0.6365333415157757 1105 76-77 0.4914417807942755 1105 78-79 0.7352785594461331 1105 80-81 0.8076612447015563 1105 82-83 0.7667038688187624 1105 84-85 0.7218573650539888 1105 86-87 0.8642348139271192 1105 88-89 0.9403136111805708 1105 90-91 0.8867079241646145 1105 92-93 0.8210034203456438 1105 94-95 0.7591830887755115 1105 96-97 1.1242866597875647 1105 98-99 1.2638165976311377 1105 100-101 1.4311732170327112 1106 1 0.17334582176771818 1106 2 0.17679413189128468 1106 3 0.20078890500067104 1106 4 0.11777252968245477 1106 5 0.05306660674698804 1106 6 0.08106276774362442 1106 7 0.17551106287590557 1106 8 0.15308833776568065 1106 9 0.1489639239170728 1106 10-11 -0.014097372367444905 1106 12-13 0.024784828554501814 1106 14-15 0.12363023504970982 1106 16-17 0.2796960202334162 1106 18-19 0.1620797450912974 1106 20-21 0.163321486571526 1106 22-23 0.2858239928620634 1106 24-25 0.2982146640289116 1106 26-27 0.2055134643675416 1106 28-29 0.30844292996035705 1106 30-31 0.3132468153484851 1106 32-33 0.2707487027817521 1106 34-35 0.21159555666567798 1106 36-37 0.19436762440428623 1106 38-39 0.05430566908217571 1106 40-41 0.032392864577040825 1106 42-43 0.06550130310213831 1106 44-45 0.026293208035383486 1106 46-47 0.2993990315442403 1106 48-49 0.40632837221677676 1106 50-51 0.5492815593461273 1106 52-53 0.36309047012112217 1106 54-55 0.3732735146289201 1106 56-57 0.46027506567169496 1106 58-59 0.335209497256848 1106 60-61 0.3060441690329583 1106 62-63 0.25128737266489054 1106 64-65 0.2201639376945579 1106 66-67 0.3748643960089737 1106 68-69 0.40256986188839505 1106 70-71 0.5319301912045233 1106 72-73 0.3867643167141068 1106 74-75 0.2754058568622746 1106 76-77 0.19384533879372512 1106 78-79 0.4395999905107182 1106 80-81 0.41395243012720684 1106 82-83 0.3068166610692842 1106 84-85 0.4242421284756901 1106 86-87 0.5685942472148362 1106 88-89 0.2419333320211976 1106 90-91 0.315528521039095 1106 92-93 0.4011790236619177 1106 94-95 0.6620289596067828 1106 96-97 0.6890381107909249 1106 98-99 0.9724471503445571 1106 100-101 1.5926302057448112 1107 1 0.3072644204936168 1107 2 0.2913957127683986 1107 3 0.2185600467014055 1107 4 0.13416550821347784 1107 5 0.18767321472412135 1107 6 0.1786670028609194 1107 7 0.18108934237237406 1107 8 0.2012730056253531 1107 9 0.20107161709582755 1107 10-11 0.26566460388337276 1107 12-13 0.23464459305287733 1107 14-15 0.22394039682624367 1107 16-17 0.2035084321948304 1107 18-19 0.29399114974539486 1107 20-21 0.35493785364247543 1107 22-23 0.35078081642114967 1107 24-25 0.2985773272595367 1107 26-27 0.3625144732190151 1107 28-29 0.3112496559799709 1107 30-31 0.20540072181697155 1107 32-33 0.29332668717200505 1107 34-35 0.18461242576032788 1107 36-37 0.10277038776513336 1107 38-39 0.2963759764215652 1107 40-41 0.21681580325157057 1107 42-43 0.31810428573376726 1107 44-45 0.44981415971859917 1107 46-47 0.3203305548927986 1107 48-49 0.22769646402912258 1107 50-51 0.2548886631703198 1107 52-53 0.12749877358638173 1107 54-55 0.12892192474419772 1107 56-57 0.15330691312237832 1107 58-59 0.15058367306122733 1107 60-61 0.26462506845492584 1107 62-63 0.16804919106819227 1107 64-65 0.25372905236562815 1107 66-67 0.3990305064919397 1107 68-69 0.2856779908635332 1107 70-71 0.25534897094033937 1107 72-73 0.4319578171713516 1107 74-75 0.2222337653313069 1107 76-77 0.18163424691789132 1107 78-79 0.3267347322327332 1107 80-81 0.45731181115908726 1107 82-83 0.4144918246216065 1107 84-85 0.5512722704869795 1107 86-87 0.77724527725562 1107 88-89 0.9037538370989111 1107 90-91 0.7073705928905945 1107 92-93 0.8044157125702114 1107 94-95 0.9445910134087647 1107 96-97 0.993540968345556 1107 98-99 1.0017941334840437 1107 100-101 1.1762992509904286 1108 1 0.14968351620933618 1108 2 0.10445329183566088 1108 3 0.05124958600875118 1108 4 0.05175486281623165 1108 5 0.04604103404094673 1108 6 0.02787641377685901 1108 7 0.05188046586392403 1108 8 0.11178480100072363 1108 9 0.1958945445132727 1108 10-11 0.06596236905804176 1108 12-13 0.11806652112831273 1108 14-15 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2316 96-97 0.5590026157483621 2316 98-99 0.24254953574088134 2316 100-101 0.8241036276179585 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 9.0 5 31.0 6 51.0 7 165.0 8 394.0 9 811.0 10 1394.0 11 2544.0 12 3908.0 13 5783.0 14 7542.0 15 11128.0 16 15616.0 17 22718.0 18 20388.0 19 11038.0 20 8693.0 21 8575.0 22 8578.0 23 9189.0 24 10569.0 25 12595.0 26 15062.0 27 18629.0 28 23106.0 29 28615.0 30 36065.0 31 45448.0 32 58746.0 33 75995.0 34 105041.0 35 150221.0 36 225119.0 37 335292.0 38 266912.0 39 25224.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.30668109622179 20.244835598561288 15.906420091033516 20.542063214183404 2 15.403077522325306 21.37463173029005 40.66124023755107 22.56105050983357 3 19.445086204866474 26.160467260989854 30.759757357279376 23.634689176864292 4 12.25243412988051 17.67339316877949 38.04027245452196 32.03390024681803 5 12.49155696288756 38.94925010551807 34.02481886860438 14.534374062989992 6 29.830148822650727 37.974629566337555 17.3382875116392 14.856934099372518 7 27.088421008414592 34.842053743669645 20.45641576910372 17.613109478812042 8 26.564744504038 35.155126753517536 19.188275959951604 19.09185278249286 9 26.73327821619031 16.890858509965955 20.681975994129324 35.69388727971441 10-11 24.636384263350557 28.065995480520122 26.376489030507607 20.921131225621714 12-13 26.154859631507843 25.524868208123756 27.76519485148452 20.555077308883877 14-15 22.990308675094624 26.177619766815024 27.528435290501076 23.303636267589276 16-17 20.53094483894147 29.903755030393985 28.084061917045517 21.481238213619026 18-19 20.366007106674584 27.38698280739741 32.11286412478623 20.134145961141776 20-21 21.68303368415894 25.99146447759465 32.540191452575854 19.785310385670556 22-23 21.183974113772873 26.105934190988574 32.45051658527364 20.25957510996491 24-25 20.801197557532532 26.31364729333922 32.71793243117672 20.167222717951525 26-27 20.56147215710884 26.494964647837214 32.75021941953588 20.193343775518063 28-29 20.40989016514371 26.751441753538906 32.722093055079085 20.116575026238294 30-31 20.5681775510295 26.438821562386433 32.74550200197429 20.24749888460978 32-33 20.53457264165774 26.541641128844912 32.54209046721644 20.38169576228091 34-35 20.936081298422415 26.47417672745464 32.50861284916805 20.08112912495489 36-37 20.92258841814436 26.315667207018333 32.43484752727058 20.32689684756673 38-39 20.65865090712715 26.620540460725312 32.493103186011425 20.227705446136113 40-41 20.868885033344238 26.612444357319887 32.35797671313262 20.16069389620325 42-43 20.632787666853282 26.672654537514955 32.01835700420158 20.676200791430173 44-45 20.916669371607288 26.755999717413264 31.712755672578712 20.614575238400736 46-47 21.179812359860207 27.157190145869492 30.978157445364975 20.684840048905325 48-49 21.12030366618105 27.336989140140744 30.735031017713226 20.80767617596498 50-51 21.29103315366163 27.61133376485092 30.31878839026756 20.778844691219888 52-53 21.36508488103671 28.01691574396369 29.803322176248837 20.814677198750765 54-55 21.240371206957235 27.819741484051352 29.826711956919528 21.113175352071888 56-57 21.355569854614213 28.03384548997294 29.709922167720322 20.90066248769252 58-59 21.551662138277837 27.65028490980455 29.903245865100477 20.89480708681714 60-61 21.301693801994528 27.753136299093878 30.296194180367987 20.648975718543607 62-63 21.191586807272664 27.693532136922183 30.372155277593738 20.742725778211415 64-65 21.269489097179925 27.674183855768746 30.266948998821917 20.78937804822941 66-67 21.19811723343677 27.890428866880917 29.821566148389273 21.08988775129304 68-69 21.10744228763166 27.873843954640954 29.905479707509624 21.113234050217763 70-71 21.36737612485751 27.764306112720284 29.791484083174698 21.076833679247507 72-73 21.30022995177568 27.689331523250715 29.77035372349397 21.240084801479632 74-75 21.118139713683625 28.36559850152654 29.28842877318532 21.227833011604513 76-77 21.164219172746417 27.888319684521186 29.737989904525143 21.209471238207254 78-79 21.245717442539107 27.98611124370624 29.490790154525303 21.277381159229353 80-81 21.22545980837552 28.35065981499467 29.261079762066906 21.1628006145629 82-83 21.246581349715274 28.259039016370696 29.31816026492511 21.176219368988917 84-85 21.166080909580405 28.141862262010992 29.279283293002866 21.412773535405737 86-87 21.116007584017048 28.120276298546525 29.277863593344954 21.485852524091474 88-89 21.206416246446185 28.024648691858893 29.203684574646495 21.565250487048427 90-91 21.231297158912245 27.93433939012793 29.289097897032367 21.545265553927454 92-93 21.25964932268175 27.88112855505759 29.389154952122936 21.470067170137725 94-95 21.091090307465826 27.916164661510095 29.34345044771541 21.64929458330867 96-97 20.979233057047516 27.903753757480754 29.369195117868585 21.74781806760315 98-99 20.97682649771396 27.934700870911765 29.070479028519834 22.017993602854435 100-101 20.922939224964804 27.759219572934597 29.50498875314433 21.81285244895627 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 957.0 1 681.5 2 566.5 3 755.5 4 611.5 5 512.5 6 1073.5 7 2656.5 8 4369.5 9 5061.0 10 4451.0 11 3702.5 12 3721.0 13 3852.0 14 4024.5 15 4233.5 16 4290.5 17 4405.0 18 4547.0 19 4732.5 20 5068.5 21 5516.5 22 6248.0 23 7308.0 24 8495.0 25 10053.0 26 12654.5 27 15478.5 28 17557.5 29 19973.0 30 22886.5 31 25331.5 32 28337.5 33 31635.5 34 35471.0 35 39205.0 36 43639.0 37 47522.0 38 48933.0 39 50266.0 40 51189.0 41 52235.5 42 52514.5 43 53722.5 44 56330.0 45 57457.0 46 57322.0 47 59381.5 48 64951.5 49 62912.5 50 55357.0 51 50035.0 52 45752.5 53 43432.5 54 41466.5 55 37974.0 56 33338.5 57 29332.5 58 25855.5 59 22792.0 60 19025.5 61 15196.5 62 11880.0 63 8551.0 64 6218.0 65 4659.5 66 3355.5 67 2485.0 68 1818.0 69 1201.0 70 802.5 71 597.5 72 441.5 73 363.5 74 300.5 75 227.5 76 146.0 77 86.0 78 63.5 79 47.0 80 29.0 81 17.5 82 11.5 83 8.0 84 5.5 85 2.5 86 1.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022212335929439875 2 0.0 3 0.0 4 6.364566168893947E-5 5 0.02405806011841912 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.1822830844469736E-5 12-13 0.004232436502314475 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.1822830844469736E-5 22-23 0.0010183305870230315 24-25 6.364566168893947E-5 26-27 5.091652935115158E-4 28-29 0.0014002045571566684 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.5911415422234867E-4 40-41 8.27393601956213E-4 42-43 0.01562500994463464 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.1822830844469736E-5 68-69 1.2729132337787894E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.364566168893947E-5 82-83 0.0024185351441796997 84-85 8.27393601956213E-4 86-87 0.0 88-89 0.0 90-91 0.011519864765698043 92-93 0.002514003636713109 94-95 0.0 96-97 0.0 98-99 0.0018775470198237143 100-101 0.020080206262860403 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1571199.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.24051100099077 #Duplication Level Percentage of deduplicated Percentage of total 1 73.97316431735658 38.64395904298964 2 14.466338754284417 15.114578576745085 3 4.96021234793664 7.7737208318890305 4 2.243513397116608 4.688091452117614 5 1.19383862074544 3.11833698002299 6 0.7065809128726979 2.2147288771209768 7 0.4575167225673474 1.673063516489173 8 0.31882545970143106 1.332448394794284 9 0.25012104322827716 1.175980599931149 >10 1.2833773021557549 12.336397856661879 >50 0.09477395822811746 3.404782143701347 >100 0.04770106417647721 4.386372963216186 >500 0.0029353451856161407 1.0999232488808919 >1k 7.338362964040352E-4 0.7427596728574132 >5k 1.2230604940067252E-4 0.5595775668718472 >10k+ 2.4461209880134504E-4 1.7352782757104932 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14655 0.9327271720514079 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12504 0.795825353758499 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8758 0.5574087050717319 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2987 0.19010959146486217 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2866 0.1824084664005005 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2291 0.1458122109293603 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.364566168893947E-5 0.0 18-19 0.0 0.0 0.0 6.364566168893947E-5 0.0 20-21 0.0 0.0 0.0 1.5911415422234867E-4 0.0 22-23 0.0 0.0 0.0 3.1822830844469734E-4 0.0 24-25 0.0 0.0 0.0 3.818739701336368E-4 0.0 26-27 0.0 0.0 0.0 6.046337860449249E-4 0.0 28-29 0.0 0.0 0.0 0.0013365588954677289 0.0 30-31 0.0 0.0 0.0 0.0028322319451578063 0.0 32-33 0.0 0.0 0.0 0.005537172566937733 0.0 34-35 0.0 0.0 0.0 0.006969199954938872 0.0 36-37 0.0 0.0 0.0 0.009801431900096677 0.0 38-39 0.0 0.0 0.0 0.01578412409885699 0.0 40-41 0.0 0.0 0.0 0.022307804421973283 0.0 42-43 0.0 0.0 0.0 0.027431280187932908 0.0 44-45 0.0 0.0 0.0 0.030358980625624123 0.0 46-47 0.0 0.0 0.0 0.03481417694384989 0.0 48-49 0.0 0.0 0.0 0.04070140065007678 0.0 50-51 0.0 0.0 0.0 0.04744784078910437 0.0 52-53 0.0 0.0 0.0 0.061322595037293176 0.0 54-55 0.0 0.0 0.0 0.06981929087276659 0.0 56-57 0.0 0.0 0.0 0.0944183391155417 0.0 58-59 0.0 0.0 0.0 0.1323829763129941 0.0 60-61 0.0 0.0 0.0 0.16897923178413427 0.0 62-63 0.0 0.0 0.0 0.1904278197733069 0.0 64-65 0.0 0.0 0.0 0.2281378743240035 0.0 66-67 0.0 0.0 0.0 0.2954749843909015 0.0 68-69 0.0 0.0 0.0 0.38473802490963904 0.0 70-71 0.0 0.0 0.0 0.47457387638357706 0.0 72-73 0.0 0.0 0.0 0.5694377351309414 0.0 74-75 0.0 0.0 0.0 0.6792265015443619 0.0 76-77 0.0 0.0 0.0 0.7642252827299407 0.0 78-79 0.0 0.0 0.0 0.872104679292693 0.0 80-81 0.0 0.0 0.0 1.01091586743627 0.0 82-83 0.0 0.0 0.0 1.1426305643015302 0.0 84-85 0.0 0.0 0.0 1.2958893176484965 0.0 86-87 0.0 0.0 0.0 1.496723203107945 0.0 88-89 0.0 0.0 0.0 1.7425545713814736 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10425 0.0 32.137257 1 GTACCGT 80 4.494701E-4 29.687141 6 GTATCAA 15020 0.0 28.91825 1 CTCCGTA 70 0.008392984 27.142532 7 ACGCACT 115 1.2807667E-4 24.786253 4 GTACATG 10345 0.0 23.611761 1 TACATGG 10280 0.0 23.198938 2 TATCAAC 18780 0.0 22.918705 2 ATCAACG 18805 0.0 22.862974 3 TCAACGC 18865 0.0 22.765076 4 AGTACTT 11500 0.0 22.613497 12-13 ACATGGG 9945 0.0 22.595078 3 CAACGCA 19055 0.0 22.538082 5 TGTACCG 190 3.283876E-7 22.50331 5 GGACCGA 360 0.0 22.430285 6 GAGTACT 10970 0.0 22.320478 12-13 AACGCAG 19435 0.0 22.167213 6 TACCGTG 130 2.9424127E-4 21.922813 7 CGCCGTA 55 0.0028770715 21.59271 90-91 AGAGTAC 17405 0.0 21.054758 10-11 >>END_MODULE