FastQCFastQC Report
Thu 26 May 2016
SRR937629_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937629_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531480
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108280.7070284953117246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87900.5739546060020372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60400.39438974064303806No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24220.15814767414527126No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21500.14038707655339933No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20450.13353096351241936No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15640.10212343615326352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83550.033.3471371
GTATCAA124550.031.0733471
GGACCGT2007.185008E-1026.128516
TATCAAC151400.025.4601042
GTACATG105450.025.3845331
ATCAACG152600.025.2877253
TACATGG104450.025.2551362
TCAACGC152900.025.2356344
CAACGCA154750.024.903245
AACGCAG157150.024.4862656
ACATGGG106050.024.0194153
CGTAGAC1000.001649141823.7617443
GAGTACT83650.023.47958812-13
GTATAGG5250.022.6406081
CATGGGA71850.022.4201554
GTACTTT89950.021.93999714-15
AGTACTT87100.021.64976712-13
ACGCAGA177700.021.467437
CGCAGAG177750.021.4613918
ATGGGAT29450.021.298765
Produced by FastQC (version 0.11.5)