##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937629_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1531480 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.625081620393345 33.0 31.0 34.0 30.0 34.0 2 31.74235967821976 33.0 31.0 34.0 30.0 34.0 3 31.75076004910283 33.0 31.0 34.0 30.0 34.0 4 35.370489983545326 37.0 35.0 37.0 33.0 37.0 5 35.374917073680365 37.0 35.0 37.0 33.0 37.0 6 35.43416172591219 37.0 35.0 37.0 33.0 37.0 7 35.38236020059028 37.0 35.0 37.0 33.0 37.0 8 35.366848408075846 37.0 35.0 37.0 33.0 37.0 9 36.95576109384386 39.0 37.0 39.0 33.0 39.0 10-11 36.83578629822132 39.0 37.0 39.0 33.0 39.0 12-13 36.70642221902996 39.0 37.0 39.0 32.0 39.0 14-15 37.78978961527412 40.0 37.5 41.0 32.5 41.0 16-17 37.7277362420665 40.0 37.5 41.0 32.5 41.0 18-19 37.71935023637266 40.0 37.5 41.0 32.0 41.0 20-21 37.66168151069554 40.0 37.0 41.0 32.0 41.0 22-23 37.46478210619793 40.0 37.0 41.0 32.0 41.0 24-25 37.2923629430355 39.5 37.0 41.0 32.0 41.0 26-27 37.23175882153211 39.5 36.5 41.0 31.5 41.0 28-29 37.275797268002194 40.0 37.0 41.0 31.5 41.0 30-31 37.071399561208764 39.0 36.5 41.0 31.0 41.0 32-33 36.799834473842296 39.0 35.5 41.0 30.5 41.0 34-35 36.76664533653721 39.0 36.0 41.0 30.0 41.0 36-37 36.65977028756497 39.0 35.5 41.0 30.0 41.0 38-39 36.51218690417113 39.0 35.0 41.0 30.0 41.0 40-41 36.25904288661948 39.0 35.0 41.0 30.0 41.0 42-43 35.97470257528665 39.0 35.0 41.0 29.5 41.0 44-45 35.67102769869669 39.0 35.0 40.0 27.5 41.0 46-47 35.24270607516912 39.0 35.0 40.0 25.5 41.0 48-49 34.80645258181628 38.5 34.5 40.0 24.0 41.0 50-51 33.84585760179695 37.5 33.5 39.5 22.5 40.5 52-53 33.9359975317993 38.0 33.5 39.5 23.0 40.5 54-55 34.286139551283725 38.0 34.0 40.0 22.5 41.0 56-57 34.084280891686475 38.0 34.0 40.0 21.5 41.0 58-59 33.84203842035156 38.0 33.5 40.0 20.0 41.0 60-61 33.513835962598264 38.0 33.0 40.0 18.5 41.0 62-63 33.15125303627863 37.0 33.0 40.0 17.5 41.0 64-65 32.778357536500636 36.5 32.0 39.5 13.5 41.0 66-67 32.35832462715804 36.0 31.5 39.0 10.0 41.0 68-69 31.859153237391283 35.5 31.0 39.0 7.5 40.0 70-71 31.411989709300805 35.0 31.0 38.5 6.5 40.0 72-73 30.941243111238805 35.0 30.5 37.5 2.0 40.0 74-75 30.47748615718129 35.0 30.0 37.0 2.0 39.0 76-77 29.9759014156241 34.5 29.0 36.5 2.0 39.0 78-79 29.433411797738138 34.0 29.0 36.0 2.0 38.5 80-81 28.89123919345992 34.0 28.5 35.5 2.0 37.5 82-83 28.348764267244757 34.0 27.5 35.0 2.0 37.0 84-85 27.860916237887533 34.0 26.5 35.0 2.0 36.5 86-87 27.380306631493717 33.0 26.0 35.0 2.0 36.0 88-89 26.863364523206307 33.0 25.0 35.0 2.0 35.5 90-91 26.33775302321937 32.5 24.5 35.0 2.0 35.0 92-93 25.739698200433565 32.0 22.0 35.0 2.0 35.0 94-95 25.082542377308222 32.0 19.0 34.0 2.0 35.0 96-97 24.331383041241153 31.5 9.5 34.0 2.0 35.0 98-99 23.372144265677647 31.0 2.0 34.0 2.0 35.0 100-101 21.012558766683206 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2505090163068111 1101 2 0.41370407255740105 1101 3 0.2367481535785565 1101 4 0.14964834704697694 1101 5 0.13369561305542277 1101 6 0.2628555225308773 1101 7 0.22563431784593035 1101 8 0.1842982783244338 1101 9 0.1996820590946271 1101 10-11 0.12405594434334688 1101 12-13 0.3232073789768606 1101 14-15 0.5874963408799019 1101 16-17 0.5356926276166902 1101 18-19 0.635340596818402 1101 20-21 0.5547046891302259 1101 22-23 0.6199889239817011 1101 24-25 0.7007454416983592 1101 26-27 0.6521945285067545 1101 28-29 0.48857622115375676 1101 30-31 0.5496456626530843 1101 32-33 0.38106615610332284 1101 34-35 0.5801839665226183 1101 36-37 0.7535380738719155 1101 38-39 0.8762610388941496 1101 40-41 0.8447869569732873 1101 42-43 0.9161917273050122 1101 44-45 1.0028003028670511 1101 46-47 1.14003083718368 1101 48-49 0.9709075586858518 1101 50-51 0.7306256416876877 1101 52-53 0.8523979867435685 1101 54-55 0.9356799777922831 1101 56-57 0.9213624567611376 1101 58-59 0.9837894712806303 1101 60-61 0.9223532149875453 1101 62-63 1.1956888824396188 1101 64-65 1.1086866274777307 1101 66-67 1.296465143266154 1101 68-69 1.1433978691138087 1101 70-71 1.2415855172387715 1101 72-73 1.1409022476563528 1101 74-75 1.2307081562515272 1101 76-77 1.213808041660986 1101 78-79 1.4012693320889618 1101 80-81 1.2929831588756961 1101 82-83 1.2047273025153622 1101 84-85 1.5421414710008534 1101 86-87 1.5466835644380446 1101 88-89 1.7249585118014146 1101 90-91 1.7967899041590023 1101 92-93 1.9420521583229338 1101 94-95 1.9654968364441352 1101 96-97 1.9126871844673907 1101 98-99 2.1609995320875726 1101 100-101 2.8925634426423663 1102 1 0.41546677659925635 1102 2 0.3059185180172861 1102 3 0.39933202001637724 1102 4 0.2801252062256623 1102 5 0.285515226842584 1102 6 0.3554986805898608 1102 7 0.295881514867979 1102 8 0.3672255606431918 1102 9 0.4657216314222339 1102 10-11 0.5227252263797695 1102 12-13 0.47120145724477425 1102 14-15 0.6481569446439508 1102 16-17 0.5899949851644877 1102 18-19 0.5776408590983735 1102 20-21 0.6053050353695184 1102 22-23 0.7049819397556547 1102 24-25 0.6281224933147627 1102 26-27 0.6877119184866274 1102 28-29 0.5760694412534662 1102 30-31 0.8255346654690641 1102 32-33 0.7917600092414006 1102 34-35 0.8128450680985324 1102 36-37 1.00091434533541 1102 38-39 1.014179070828412 1102 40-41 0.9810341614253559 1102 42-43 0.978966173953701 1102 44-45 1.109367332862071 1102 46-47 1.1078216670434742 1102 48-49 1.1666367787558443 1102 50-51 1.2429715614879768 1102 52-53 1.2055908554029742 1102 54-55 1.3339777584430053 1102 56-57 1.3857549776952425 1102 58-59 1.1092334154365489 1102 60-61 1.3758161651543546 1102 62-63 1.4352116146094147 1102 64-65 1.2826198612486976 1102 66-67 1.4154988310118028 1102 68-69 1.4762250318091255 1102 70-71 1.430006376298575 1102 72-73 1.4248184666638473 1102 74-75 1.4950091020940555 1102 76-77 1.3190562417367708 1102 78-79 1.3960237325228775 1102 80-81 1.628343032268024 1102 82-83 1.524668010894203 1102 84-85 1.513767123914537 1102 86-87 1.6105576551884049 1102 88-89 1.699797651701406 1102 90-91 1.8683466435656406 1102 92-93 1.955013841670013 1102 94-95 2.0993822208366204 1102 96-97 2.1768386010021743 1102 98-99 2.3593041855139276 1102 100-101 2.557298302103394 1103 1 0.27997940911509644 1103 2 0.2581506884130462 1103 3 0.33052380337335663 1103 4 0.2209726907379448 1103 5 0.23033752563441112 1103 6 0.3789880220423143 1103 7 0.3683155763958652 1103 8 0.3813147593354387 1103 9 0.2359565688985512 1103 10-11 0.2835732396335615 1103 12-13 0.4968035616359714 1103 14-15 0.622103206882116 1103 16-17 0.5195080142364574 1103 18-19 0.41642977667638803 1103 20-21 0.40482520245183196 1103 22-23 0.4734154108555231 1103 24-25 0.5399008713120637 1103 26-27 0.5071209176670735 1103 28-29 0.43024451637882066 1103 30-31 0.29606283417285084 1103 32-33 0.2577158857626287 1103 34-35 0.23359905348764443 1103 36-37 0.34106168796871117 1103 38-39 0.46002756876190887 1103 40-41 0.6452934386357185 1103 42-43 0.5143986845064958 1103 44-45 0.6417798481735417 1103 46-47 0.750329840505934 1103 48-49 0.539307011490223 1103 50-51 0.5069301169661742 1103 52-53 0.41782857758455805 1103 54-55 0.34384231249361363 1103 56-57 0.4096091507875883 1103 58-59 0.46994021064614344 1103 60-61 0.4961887957314701 1103 62-63 0.6081799253684252 1103 64-65 0.6744543299075403 1103 66-67 0.6622393599295577 1103 68-69 0.656994703196272 1103 70-71 0.8679241922436596 1103 72-73 0.8869920789377055 1103 74-75 0.7467510850509527 1103 76-77 0.7588246529566653 1103 78-79 0.9235691822615877 1103 80-81 0.8698739151782178 1103 82-83 1.0878521803734778 1103 84-85 1.1487094470284767 1103 86-87 1.132479278072335 1103 88-89 1.1749910829694166 1103 90-91 1.1265729801801108 1103 92-93 1.2049014840997536 1103 94-95 1.3435552691195305 1103 96-97 1.6655501901347733 1103 98-99 1.9318287940249022 1103 100-101 2.346735809263432 1104 1 0.2174269438428844 1104 2 0.16514389229233828 1104 3 0.1608412588780439 1104 4 0.31915995873206526 1104 5 0.24642427688080204 1104 6 0.2398667964015786 1104 7 0.05960493347158291 1104 8 0.206793065026595 1104 9 0.21967793228093058 1104 10-11 0.33998234097762037 1104 12-13 0.31207274259297435 1104 14-15 0.6294656709848212 1104 16-17 0.7301851573534961 1104 18-19 0.5919150127646944 1104 20-21 0.5520186081789404 1104 22-23 0.5069589662358922 1104 24-25 0.5853393705345624 1104 26-27 0.5658140180235591 1104 28-29 0.46201670556133934 1104 30-31 0.5103961949390055 1104 32-33 0.488445483323126 1104 34-35 0.5445696556092017 1104 36-37 0.5549200999548347 1104 38-39 0.658235627947505 1104 40-41 0.6012068000082849 1104 42-43 0.5298192513263515 1104 44-45 0.43448647146747277 1104 46-47 0.635886676259851 1104 48-49 0.5079913622203023 1104 50-51 0.5642018361811338 1104 52-53 0.549058463189688 1104 54-55 0.5715918392774242 1104 56-57 0.5587419848435573 1104 58-59 0.60087685382166 1104 60-61 0.5785147669616677 1104 62-63 0.5448921882730318 1104 64-65 0.5063306916222956 1104 66-67 0.5417576540122937 1104 68-69 0.7098153748383957 1104 70-71 0.6889754901771035 1104 72-73 0.7828468351393276 1104 74-75 0.7154211418368952 1104 76-77 0.803489546596726 1104 78-79 0.7941433656658887 1104 80-81 0.9255826132597953 1104 82-83 1.0106748445047735 1104 84-85 1.1027951327262997 1104 86-87 1.3091032650431913 1104 88-89 1.4058343823582433 1104 90-91 1.224301325937624 1104 92-93 1.4090330836787324 1104 94-95 1.6859768798304593 1104 96-97 1.962630760333198 1104 98-99 1.9948131096698418 1104 100-101 2.6329850350081294 1105 1 0.13963118159275822 1105 2 0.0035094731464866413 1105 3 0.23857573586710856 1105 4 0.12453476007966913 1105 5 0.0727892801423522 1105 6 0.09646977956659697 1105 7 0.2416645547958325 1105 8 0.1669270027053642 1105 9 0.20361363816540745 1105 10-11 0.21773881349874102 1105 12-13 0.19530480133279582 1105 14-15 0.22875136188379486 1105 16-17 0.25853546726382604 1105 18-19 0.3170743334553592 1105 20-21 0.33443074568291564 1105 22-23 0.38052243551896225 1105 24-25 0.3538811003404376 1105 26-27 0.31929069730267656 1105 28-29 0.4344066460474707 1105 30-31 0.2719780598746908 1105 32-33 0.30338015575340904 1105 34-35 0.3878475019947629 1105 36-37 0.43727863384348353 1105 38-39 0.4694077631343916 1105 40-41 0.526653489806634 1105 42-43 0.6596558547460702 1105 44-45 0.6665389148604604 1105 46-47 0.5333709162932294 1105 48-49 0.562946821267893 1105 50-51 0.5605089794400442 1105 52-53 0.4259600902713956 1105 54-55 0.5840149073855017 1105 56-57 0.5109913218293869 1105 58-59 0.46244919015852304 1105 60-61 0.5600324334875708 1105 62-63 0.5036213922110804 1105 64-65 0.56229588831021 1105 66-67 0.7051527651275151 1105 68-69 0.7821649342141548 1105 70-71 0.8459788843731353 1105 72-73 0.9418375928182421 1105 74-75 0.8613482745010153 1105 76-77 1.0342707405847271 1105 78-79 1.1256834055097045 1105 80-81 1.0864501271182405 1105 82-83 1.1844345105756595 1105 84-85 1.204479973691047 1105 86-87 1.3591857063121822 1105 88-89 1.3704404650463324 1105 90-91 1.4350178933585447 1105 92-93 1.7146960516819512 1105 94-95 1.8024043940582963 1105 96-97 1.892766402245936 1105 98-99 2.007248140996687 1105 100-101 2.110387983920564 1106 1 0.13528290025219292 1106 2 0.18942076128154994 1106 3 0.210480924535851 1106 4 0.20166275075980877 1106 5 0.19573540976796266 1106 6 0.026864711353660198 1106 7 0.14400012767818282 1106 8 0.029404566678444155 1106 9 0.17592364407689587 1106 10-11 0.11723695554425007 1106 12-13 0.10272449952546481 1106 14-15 -0.036352814659394994 1106 16-17 0.031091043064819246 1106 18-19 0.2608727559048276 1106 20-21 0.31849240652191924 1106 22-23 0.2809340959045059 1106 24-25 0.14567930512849614 1106 26-27 0.2742741542110707 1106 28-29 0.2770279654878536 1106 30-31 0.3085000366690167 1106 32-33 0.4052397179736502 1106 34-35 0.37063926515936174 1106 36-37 0.274863441406886 1106 38-39 0.3243354030828485 1106 40-41 0.4574849811253259 1106 42-43 0.4787314038670587 1106 44-45 0.5395497657764068 1106 46-47 0.47911077133402813 1106 48-49 0.6435019485544728 1106 50-51 0.7095930288986594 1106 52-53 0.7033374022024503 1106 54-55 0.8539964721236899 1106 56-57 0.7086041780335179 1106 58-59 0.8342352834083044 1106 60-61 0.7980987756958271 1106 62-63 0.9638854056071509 1106 64-65 1.0324305168388506 1106 66-67 0.9835520138658538 1106 68-69 0.7991761215159237 1106 70-71 0.9129169819629475 1106 72-73 0.9809752166646994 1106 74-75 1.1366926371117323 1106 76-77 1.1623172789026164 1106 78-79 1.1410901643911515 1106 80-81 1.0460308568281143 1106 82-83 0.8059361599379713 1106 84-85 0.9996265777195994 1106 86-87 1.040546922841589 1106 88-89 1.2523008187626132 1106 90-91 1.4337426365964099 1106 92-93 1.3990239355101188 1106 94-95 1.6863554103474492 1106 96-97 1.9532810148292405 1106 98-99 2.03139660256749 1106 100-101 2.5112918559026802 1107 1 0.18065510551172181 1107 2 0.22390790793770776 1107 3 0.36222326670072746 1107 4 0.3804063733344165 1107 5 0.34561071755876327 1107 6 0.3704482827118767 1107 7 0.3977170586345551 1107 8 0.3155544731100406 1107 9 0.41529722823921134 1107 10-11 0.44304883380682725 1107 12-13 0.44891498509623773 1107 14-15 0.5156794910630467 1107 16-17 0.6101382212387634 1107 18-19 0.6052005407959911 1107 20-21 0.5278673695870211 1107 22-23 0.561164963628336 1107 24-25 0.5894567108213948 1107 26-27 0.5768127142425925 1107 28-29 0.500188804648694 1107 30-31 0.4965187503497006 1107 32-33 0.5189722108329065 1107 34-35 0.6364418850746532 1107 36-37 0.6347923294202857 1107 38-39 0.8636697870087815 1107 40-41 0.9479890685871624 1107 42-43 0.9182208158635135 1107 44-45 0.8546272798116732 1107 46-47 0.9198110569638942 1107 48-49 0.8488648954667184 1107 50-51 0.9170962299229828 1107 52-53 0.8078720225117237 1107 54-55 0.8816700661353138 1107 56-57 0.9866037840612947 1107 58-59 1.0195808955426457 1107 60-61 1.0861105948625749 1107 62-63 1.1839039223017167 1107 64-65 1.2003280840603665 1107 66-67 1.202307849579448 1107 68-69 1.2735937320743709 1107 70-71 1.417953325900701 1107 72-73 1.4267998990245978 1107 74-75 1.5796271392293306 1107 76-77 1.5700058438587838 1107 78-79 1.6472359341143878 1107 80-81 1.539016772321073 1107 82-83 1.3446096554565337 1107 84-85 1.644573489962287 1107 86-87 1.8449024827746108 1107 88-89 1.8804262989162588 1107 90-91 1.847672903512592 1107 92-93 1.9364568124922918 1107 94-95 2.151236332242455 1107 96-97 2.148883693840368 1107 98-99 2.7209460953282516 1107 100-101 3.2954309280980887 1108 1 0.07683779781941524 1108 2 0.17819636947617212 1108 3 0.09270403593149723 1108 4 0.15538714256518205 1108 5 0.11299533294337749 1108 6 0.1448040519426428 1108 7 0.1535439817114792 1108 8 0.19497755003311568 1108 9 0.2070279974699787 1108 10-11 0.13553441072991035 1108 12-13 0.18477075432700474 1108 14-15 0.07719872183228205 1108 16-17 0.3071045673926065 1108 18-19 0.1998880407959902 1108 20-21 0.2619587157408674 1108 22-23 0.2502322713206411 1108 24-25 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-0.30128333067889557 2316 96-97 -0.05328333295306109 2316 98-99 -0.07560662723008704 2316 100-101 -0.014052434045385809 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6395.0 3 1220.0 4 1132.0 5 1308.0 6 1512.0 7 1776.0 8 2364.0 9 2856.0 10 3672.0 11 4837.0 12 6528.0 13 8469.0 14 9695.0 15 12620.0 16 16055.0 17 21591.0 18 18442.0 19 12062.0 20 11544.0 21 12130.0 22 13182.0 23 14387.0 24 15965.0 25 18138.0 26 20650.0 27 24308.0 28 28765.0 29 33970.0 30 40851.0 31 50848.0 32 63691.0 33 80795.0 34 108241.0 35 148967.0 36 207917.0 37 273626.0 38 206282.0 39 24685.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.00866080416326 19.69488086482295 15.137545280274336 21.15891305073945 2 14.785120324891285 20.786860813917627 41.44040200188979 22.9876168593013 3 19.319994945563323 26.192195609318237 30.135114520309735 24.352694924808706 4 11.916367502232278 17.008774617239727 38.35995045784596 32.71490742268203 5 12.595571754749784 39.05899788432937 33.89831950815895 14.447110852761893 6 29.970542317119612 37.30185422438353 17.892041714486872 14.835561744009986 7 27.47447519162008 34.43423415760772 20.51877271552556 17.572517935246644 8 26.413118941941445 35.03562611198685 19.516526602946016 19.034728343125686 9 26.809838833159933 17.04553633942535 20.805376154112928 35.339248673301796 10-11 24.577846369483687 27.91398146388035 26.646383617910026 20.86178854872594 12-13 25.884322115136094 25.421541215800183 27.750920817844595 20.94321585121913 14-15 22.920613712376223 26.14117399957011 27.25803185292469 23.68018043512897 16-17 20.815016850019614 29.912751543917192 27.646758420020724 21.62547318604247 18-19 20.684187160514668 27.745187622131297 31.180812713530404 20.38981250382363 20-21 22.058024016931576 26.478094608784403 31.239021135030637 20.22486023925339 22-23 21.44129062561534 26.671259035092366 31.208381661142088 20.679068678150202 24-25 20.98434139116691 26.832932535051313 31.498689010189608 20.684037063592164 26-27 20.742365506976135 27.035019709832014 31.531380342509447 20.6912344406824 28-29 20.668810158936232 27.269030092105602 31.480020664949386 20.582139084008777 30-31 20.805141430899425 27.04462927696011 31.447882454284702 20.70234683785576 32-33 20.68153650649067 27.08006967701745 31.358747645914065 20.87964617057782 34-35 21.085458937055826 27.08317997972297 31.217200729815964 20.614160353405236 36-37 21.04932601647079 26.92672800688769 31.199215713982902 20.824730262658615 38-39 20.81385649564122 27.24257045190625 31.220261363078784 20.723311689373745 40-41 21.007772806266654 27.21555071524668 31.14166342568045 20.635013052806215 42-43 20.736019104633854 27.171565500433452 30.898670199715394 21.193745195217296 44-45 20.92749616206567 27.41905990317905 30.59163233815824 21.06181159659704 46-47 21.211040710304843 27.7096941026994 29.927335856334814 21.151929330660945 48-49 21.1416438611615 27.785333265721075 29.823482936356694 21.24953993676073 50-51 21.26636533028398 28.05503887762831 29.558743417468676 21.119852374619036 52-53 21.29386633717148 28.417421796158887 29.096285718399418 21.192426148270215 54-55 21.234746577518187 28.103133598733045 29.184306456514765 21.47781336723401 56-57 21.38804000010466 28.464425600276293 28.946242664161865 21.201291735457183 58-59 21.480339693260884 28.023091449387127 29.282452604705288 21.2141162526467 60-61 21.335869735512695 28.073406078272384 29.62147926193575 20.96924492427917 62-63 21.215414095328313 28.12059018062228 29.712786439950566 20.95120928409884 64-65 21.200919708649906 28.171735639364314 29.562844032196345 21.064500619789435 66-67 21.159699784841006 28.311921011986495 29.147273028194398 21.3811061749781 68-69 21.062416998671978 28.350865830602057 29.241261554766158 21.345455615959807 70-71 21.31786039026879 28.256088546402474 29.12689950218815 21.299151561140583 72-73 21.176623740238195 28.181246083176003 29.222516161191948 21.419614015393854 74-75 21.003549551105426 28.72863138834156 28.810893837937414 21.4569252226156 76-77 21.134741842861214 28.422000647081703 28.944082392017012 21.499175118040068 78-79 21.176024117867406 28.405135679361916 28.88667513998145 21.532165062789225 80-81 21.13761644356325 28.787684992212835 28.61669773791092 21.458000826312997 82-83 21.275918536648238 28.620253570351412 28.714038878868113 21.389789014132237 84-85 21.184113860825864 28.58757309778929 28.64965592766729 21.578657113717558 86-87 21.12562676655025 28.587816335362344 28.73481555915149 21.55174133893592 88-89 21.274671986114306 28.44758467483535 28.564661809334048 21.7130815297163 90-91 21.227724054863998 28.459299672450335 28.678505734981474 21.634470537704196 92-93 21.37938095566764 28.33555823277671 28.710427347519698 21.57463346403595 94-95 21.121433267802175 28.403863830399693 28.705885046793174 21.768817855004954 96-97 21.02564622528422 28.45684041618246 28.669569560369577 21.847943798163737 98-99 21.06377014395278 28.480794247514783 28.480467149006127 21.974968459526302 100-101 21.299187823634227 27.883762490743962 28.995762501210155 21.821287184411656 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2541.0 1 1511.0 2 517.0 3 603.5 4 549.5 5 532.5 6 946.5 7 1996.0 8 3283.0 9 3930.5 10 3544.5 11 3080.5 12 3176.5 13 3344.0 14 3500.5 15 3592.5 16 3640.5 17 3771.0 18 3948.5 19 4111.0 20 4389.5 21 4916.5 22 5705.5 23 6961.0 24 8204.5 25 9559.0 26 11991.5 27 15030.5 28 17015.5 29 19175.5 30 22103.5 31 24542.5 32 27802.0 33 31317.0 34 34966.5 35 38212.5 36 42353.5 37 46247.0 38 47478.5 39 48560.5 40 49623.5 41 51005.5 42 51591.5 43 52527.5 44 54633.0 45 55730.5 46 56544.0 47 58358.0 48 62524.5 49 61425.0 50 54454.0 51 48665.0 52 44544.5 53 42446.0 54 40740.5 55 37311.0 56 32923.0 57 29210.5 58 25953.0 59 23085.5 60 19484.0 61 15690.5 62 12251.0 63 8917.5 64 6550.5 65 4933.0 66 3577.5 67 2620.5 68 1907.5 69 1265.0 70 897.5 71 677.5 72 500.5 73 415.5 74 327.5 75 236.5 76 168.5 77 114.5 78 74.5 79 47.0 80 35.5 81 28.5 82 18.5 83 18.5 84 15.0 85 5.5 86 3.5 87 3.0 88 3.5 89 3.5 90 3.0 91 2.0 92 1.0 93 1.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3004283438242745 2 0.28227596834434665 3 0.2683025570036827 4 0.2541332567189908 5 0.2502154778384308 6 0.208034058557735 7 0.20013320448193905 8 0.21201713375297102 9 0.212213022696999 10-11 0.21381278240656096 12-13 0.21018883694204302 14-15 0.20728315093896102 16-17 0.20525896518400502 18-19 0.206042520960117 20-21 0.19425653616109909 22-23 0.1865515710293311 24-25 0.1870086452320631 26-27 0.19948024133517903 28-29 0.21472693081202496 30-31 0.208262595659101 32-33 0.21456369002533496 34-35 0.20565074307206102 36-37 0.19301590618225506 38-39 0.19295060986757906 40-41 0.1881513307388931 42-43 0.19948024133517903 44-45 0.19513803640922506 46-47 0.19650925901742106 48-49 0.20617311358946902 50-51 0.21466163449734896 52-53 0.2276229529605349 54-55 0.20199414945020502 56-57 0.17525530859038316 58-59 0.17558179016376316 60-61 0.18318881082351712 62-63 0.1913834983153551 64-65 0.17858542063885915 66-67 0.18563742262386712 68-69 0.1887063494136391 70-71 0.18217671794603912 72-73 0.18400501475696712 74-75 0.18518034842113512 76-77 0.20108000104474105 78-79 0.20584663201608902 80-81 0.19611748112936508 82-83 0.19517068456656309 84-85 0.18785749732285112 86-87 0.1893919607177371 88-89 0.19592159218533708 90-91 0.19650925901742106 92-93 0.1923955911928331 94-95 0.1922976467208191 96-97 0.18122992138323712 98-99 0.1886084049416251 100-101 0.17943427272964713 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1531480.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.987191626613566 #Duplication Level Percentage of deduplicated Percentage of total 1 75.96557880544094 41.77133838801395 2 13.416713697369467 14.754948141533319 3 4.627397942397837 7.633428521736817 4 2.0345169096068587 4.474894847045519 5 1.1002630650784502 3.0250187999576967 6 0.6338935221586489 2.0913614744283984 7 0.4136651368084699 1.5922398902855655 8 0.2902132556294811 1.276640951990533 9 0.2187043837713073 1.0823345874011978 >10 1.1662999159778908 11.798758473036827 >50 0.0878962590948329 3.3138235303414727 >100 0.04164463633867189 4.008878606259274 >500 0.0021416468847576986 0.7886859693917876 >1k 7.138822949192328E-4 0.7089995728161596 >5k 2.3796076497307762E-4 0.9702374886835513 >10k+ 1.1898038248653881E-4 0.7084107570779159 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10828 0.7070284953117246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8790 0.5739546060020372 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6040 0.39438974064303806 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2422 0.15814767414527126 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2150 0.14038707655339933 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2045 0.13353096351241936 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1564 0.10212343615326352 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.591297307179983E-4 0.0 24-25 0.0 0.0 0.0 8.488520907879959E-4 0.0 26-27 0.0 0.0 0.0 0.0012079818215059942 0.0 28-29 0.0 0.0 0.0 0.002285371013659989 0.0 30-31 0.0 0.0 0.0 0.004407501240629979 0.0 32-33 0.0 0.0 0.0 0.007607020659753963 0.0 34-35 0.0 0.0 0.0 0.009957687988089953 0.0 36-37 0.0 0.0 0.0 0.013516337137931937 0.0 38-39 0.0 0.0 0.0 0.019458301773447907 0.0 40-41 0.0 0.0 0.0 0.025792044297019874 0.0 42-43 0.0 0.0 0.0 0.03179930524721185 0.0 44-45 0.0 0.0 0.0 0.03473763940763183 0.0 46-47 0.0 0.0 0.0 0.039569566693655814 0.0 48-49 0.0 0.0 0.0 0.04551153132917178 0.0 50-51 0.0 0.0 0.0 0.05253088515684175 0.0 52-53 0.0 0.0 0.0 0.0655574999347037 0.0 54-55 0.0 0.0 0.0 0.07388278005589365 0.0 56-57 0.0 0.0 0.0 0.09709561992321153 0.0 58-59 0.0 0.0 0.0 0.13607751978478333 0.0 60-61 0.0 0.0 0.0 0.1707825110350772 0.0 62-63 0.0 0.0 0.0 0.19402799905973306 0.0 64-65 0.0 0.0 0.0 0.2329772507639669 0.0 66-67 0.0 0.0 0.0 0.2990244730587406 0.0 68-69 0.0 0.0 0.0 0.3880559981194661 0.0 70-71 0.0 0.0 0.0 0.4744430224358137 0.0 72-73 0.0 0.0 0.0 0.5677188079504794 0.0 74-75 0.0 0.0 0.0 0.6790490244730587 0.0 76-77 0.0 0.0 0.0 0.7629874369890564 0.0 78-79 0.0 0.0 0.0 0.8690939483375558 0.0 80-81 0.0 0.0 0.0 1.007718024394703 0.0 82-83 0.0 0.0 0.0 1.1357967456316764 0.0 84-85 0.0 0.0 0.0 1.2866312325332356 0.0 86-87 0.0 0.0 0.0 1.4822916394598689 0.0 88-89 0.0 6.529631467599969E-5 0.0 1.7269569305508397 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8355 0.0 33.347137 1 GTATCAA 12455 0.0 31.073347 1 GGACCGT 200 7.185008E-10 26.12851 6 TATCAAC 15140 0.0 25.460104 2 GTACATG 10545 0.0 25.384533 1 ATCAACG 15260 0.0 25.287725 3 TACATGG 10445 0.0 25.255136 2 TCAACGC 15290 0.0 25.235634 4 CAACGCA 15475 0.0 24.90324 5 AACGCAG 15715 0.0 24.486265 6 ACATGGG 10605 0.0 24.019415 3 CGTAGAC 100 0.0016491418 23.761744 3 GAGTACT 8365 0.0 23.479588 12-13 GTATAGG 525 0.0 22.640608 1 CATGGGA 7185 0.0 22.420155 4 GTACTTT 8995 0.0 21.939997 14-15 AGTACTT 8710 0.0 21.649767 12-13 ACGCAGA 17770 0.0 21.46743 7 CGCAGAG 17775 0.0 21.461391 8 ATGGGAT 2945 0.0 21.29876 5 >>END_MODULE