FastQCFastQC Report
Thu 26 May 2016
SRR937629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937629_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531480
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142500.9304724841329955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122730.8013816700185441No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86400.5641601588006373No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29190.1905999425392431No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29180.1905346462245671No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20990.13705696450492336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106500.032.621761
GTATACG804.479443E-429.7043251
GTATCAA153450.028.6493341
GTATAGG5600.024.6121541
GAGTACT107950.024.46770512-13
TACATGG95300.024.2261522
GTACATG98200.024.1022451
GTACCGG1201.704617E-423.7634581
ATCAACG183550.023.7590663
TATCAAC184400.023.6495482
TCAACGC185100.023.5344454
CAACGCA185850.023.4650345
AACGCAG190050.022.9927066
GTACTTT115200.022.73857314-15
ACATGGG97650.022.280983
AGTACTT111400.021.81230412-13
ACCGTGC1353.7939395E-421.1134178
CATGGGA67200.020.9249064
CCCGCGA1259.509677E-920.89887220-21
CGCAGAG210750.020.6475728
Produced by FastQC (version 0.11.5)