##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937629_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1531480 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26150259879332 33.0 31.0 34.0 31.0 34.0 2 32.46695484106877 34.0 31.0 34.0 31.0 34.0 3 32.51567046255909 34.0 31.0 34.0 31.0 34.0 4 36.12328335988717 37.0 35.0 37.0 35.0 37.0 5 36.06272363987777 37.0 35.0 37.0 35.0 37.0 6 36.120132159740905 37.0 35.0 37.0 35.0 37.0 7 36.078868153681405 37.0 35.0 37.0 35.0 37.0 8 36.04917204272991 37.0 35.0 37.0 35.0 37.0 9 37.74098649672212 39.0 37.0 39.0 35.0 39.0 10-11 37.66827741792253 39.0 37.0 39.0 35.0 39.0 12-13 37.566939169953244 39.0 37.0 39.0 35.0 39.0 14-15 38.751543931360516 40.0 38.0 41.0 35.0 41.0 16-17 38.725240943401154 40.0 38.0 41.0 34.5 41.0 18-19 38.772112270483454 40.0 38.0 41.0 35.0 41.0 20-21 38.7061825815551 40.0 38.0 41.0 34.0 41.0 22-23 38.65650481886802 40.0 38.0 41.0 34.0 41.0 24-25 38.66853990910753 40.0 38.0 41.0 34.0 41.0 26-27 38.49574823046987 40.0 38.0 41.0 34.0 41.0 28-29 38.445867069762585 40.0 38.0 41.0 34.0 41.0 30-31 38.38448559563298 40.0 38.0 41.0 34.0 41.0 32-33 38.192383511374615 40.0 37.0 41.0 34.0 41.0 34-35 38.022150795309116 40.0 37.0 41.0 33.5 41.0 36-37 37.84091597670228 40.0 37.0 41.0 33.0 41.0 38-39 37.6860798051558 40.0 37.0 41.0 33.0 41.0 40-41 37.494186669104394 40.0 37.0 41.0 33.0 41.0 42-43 37.22681360514012 40.0 36.0 41.0 32.5 41.0 44-45 36.838917256510044 40.0 36.0 41.0 31.5 41.0 46-47 36.362866638806906 39.5 36.0 41.0 30.5 41.0 48-49 35.90736803614804 39.0 35.5 41.0 29.0 41.0 50-51 35.62485243032883 39.0 35.0 41.0 28.0 41.0 52-53 35.35814832710842 39.0 35.0 40.0 27.0 41.0 54-55 35.140526810666806 39.0 35.0 40.0 26.5 41.0 56-57 34.87630298795936 38.0 35.0 40.0 26.0 41.0 58-59 34.63435206466947 38.0 34.0 40.0 26.0 41.0 60-61 34.304973946770446 38.0 34.0 40.0 24.5 41.0 62-63 33.91935546007783 37.5 34.0 40.0 23.0 41.0 64-65 33.533540431478045 37.0 33.0 39.5 22.0 41.0 66-67 33.11724247133492 36.0 33.0 39.0 21.5 40.5 68-69 32.66205435265233 36.0 32.0 39.0 20.0 40.0 70-71 32.23996983310262 35.0 32.0 38.5 18.5 40.0 72-73 31.770224880507747 35.0 31.0 37.5 17.5 40.0 74-75 31.29760917543814 35.0 31.0 37.0 13.0 39.0 76-77 30.01832736960326 33.5 29.5 35.5 11.0 38.5 78-79 30.613554535482017 34.0 31.0 36.0 9.0 38.5 80-81 30.434365123932405 34.0 31.0 36.0 7.0 37.5 82-83 30.04122188993653 34.0 30.0 35.0 4.0 37.0 84-85 29.629820500430956 34.0 30.0 35.0 2.0 36.5 86-87 29.30703600438791 34.0 29.5 35.0 2.0 36.0 88-89 28.894400514534958 34.0 29.0 35.0 2.0 36.0 90-91 28.389330908663517 34.0 29.0 35.0 2.0 35.0 92-93 27.936998197821715 33.5 28.0 35.0 2.0 35.0 94-95 27.452934742863114 33.0 27.0 35.0 2.0 35.0 96-97 26.937132708229946 33.0 26.0 35.0 2.0 35.0 98-99 26.131307950479275 32.5 24.5 34.5 2.0 35.0 100-101 23.69955859691279 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32531655584820385 1101 2 0.30024877102101755 1101 3 0.3022939808206786 1101 4 0.19177544317241058 1101 5 0.18402264138176605 1101 6 0.19773468388362403 1101 7 0.21806604994445422 1101 8 0.22242769480146762 1101 9 0.2973470244740781 1101 10-11 0.26359626821322735 1101 12-13 0.27579263357795014 1101 14-15 0.41047560550590134 1101 16-17 0.314989334633502 1101 18-19 0.3261126203253113 1101 20-21 0.3260562678797285 1101 22-23 0.2003160807967106 1101 24-25 0.1974354238251621 1101 26-27 0.0915942371711651 1101 28-29 0.25453250060036936 1101 30-31 0.26095046837473035 1101 32-33 0.17658873186422142 1101 34-35 0.23538510798291412 1101 36-37 0.11352170831423791 1101 38-39 0.20289162970531294 1101 40-41 0.06730726404499165 1101 42-43 0.22063366073384572 1101 44-45 0.03882934556713025 1101 46-47 0.23775275741555646 1101 48-49 0.3434912499235807 1101 50-51 0.2877135846587393 1101 52-53 0.3019285258391946 1101 54-55 0.268310005524377 1101 56-57 0.18912838426733458 1101 58-59 0.27158952824081695 1101 60-61 0.3266782377936366 1101 62-63 0.1973122259031257 1101 64-65 0.21433112653225095 1101 66-67 0.24548713063661154 1101 68-69 0.3037884315142563 1101 70-71 0.27126668188444114 1101 72-73 0.3380858623328926 1101 74-75 0.27284040956921984 1101 76-77 0.20662981005051861 1101 78-79 0.2691017756465257 1101 80-81 0.1873234977726952 1101 82-83 0.19723282734757674 1101 84-85 0.19439904690946008 1101 86-87 0.4307797499491173 1101 88-89 0.6173520102307215 1101 90-91 0.732451773640701 1101 92-93 0.6945569434606504 1101 94-95 0.5616660587010038 1101 96-97 0.8149225488069725 1101 98-99 1.1254836257861527 1101 100-101 1.8047508244819923 1102 1 0.20728313558354472 1102 2 0.2658718902952515 1102 3 0.24851544022672556 1102 4 0.1310684017351491 1102 5 0.1632111337548423 1102 6 0.18577048175714594 1102 7 0.15125338323321103 1102 8 0.21287825168204932 1102 9 0.23665670059916977 1102 10-11 0.24697436746352963 1102 12-13 0.23134249277892138 1102 14-15 0.20975340681210497 1102 16-17 0.32416913387414326 1102 18-19 0.2905032837524857 1102 20-21 0.29709309109707505 1102 22-23 0.3589638900530403 1102 24-25 0.3986610068965888 1102 26-27 0.34258707462445415 1102 28-29 0.2756449243497059 1102 30-31 0.14148583902166223 1102 32-33 0.1370479818772239 1102 34-35 0.27440389852300484 1102 36-37 0.45312784254512906 1102 38-39 0.4231808063839395 1102 40-41 0.5015450175018046 1102 42-43 0.5397329184740798 1102 44-45 0.47036256893604644 1102 46-47 0.3167720309074511 1102 48-49 0.4211443270695625 1102 50-51 0.40966820204946686 1102 52-53 0.2921747569576425 1102 54-55 0.4401823353581662 1102 56-57 0.37295005617217214 1102 58-59 0.47103747718167455 1102 60-61 0.4152358093633737 1102 62-63 0.3972654167285299 1102 64-65 0.5812416838586287 1102 66-67 0.44663655592397333 1102 68-69 0.41665426750382295 1102 70-71 0.3362775668750402 1102 72-73 0.3421612325340284 1102 74-75 0.4347147083603531 1102 76-77 0.3432540884090791 1102 78-79 0.15813152547748643 1102 80-81 0.14587880318398305 1102 82-83 0.18226782277810827 1102 84-85 0.3261325095252765 1102 86-87 0.305252217981419 1102 88-89 0.43283191572076873 1102 90-91 0.20058995729612406 1102 92-93 0.5329046167474729 1102 94-95 0.7555656344464161 1102 96-97 0.6526312397103915 1102 98-99 0.7877702911154145 1102 100-101 1.3146764127544373 1103 1 0.12048625163349413 1103 2 0.10984423711443014 1103 3 0.16564490258473086 1103 4 0.0764344230434304 1103 5 0.07993170268070315 1103 6 0.13454336084277685 1103 7 0.13842302994575562 1103 8 0.1323293298741035 1103 9 0.11899056561146182 1103 10-11 0.21657327296861695 1103 12-13 0.2724589745329382 1103 14-15 0.25219438604137423 1103 16-17 0.1355368559676151 1103 18-19 0.2746648319076215 1103 20-21 0.16317762805170588 1103 22-23 0.2274136640160478 1103 24-25 0.1170742095720243 1103 26-27 0.07183330954429579 1103 28-29 0.06479404707942393 1103 30-31 0.047850344604299266 1103 32-33 0.20911104311429796 1103 34-35 0.1865869840821901 1103 36-37 0.16729833524408377 1103 38-39 0.2519372944828504 1103 40-41 0.22941233863296162 1103 42-43 0.3322908817617858 1103 44-45 0.49754539012447907 1103 46-47 0.44900609596027863 1103 48-49 0.3838713554541684 1103 50-51 0.47214782347054296 1103 52-53 0.45889224155803987 1103 54-55 0.40708598103085336 1103 56-57 0.4448022817332884 1103 58-59 0.34530296710473607 1103 60-61 0.45149095357761837 1103 62-63 0.3523102716330513 1103 64-65 0.37675377000824284 1103 66-67 0.46996110627338084 1103 68-69 0.5573061176584773 1103 70-71 0.38754412309348396 1103 72-73 0.3187271886308558 1103 74-75 0.29227725407446314 1103 76-77 0.4439368258801082 1103 78-79 0.44132834069114324 1103 80-81 0.5349946592605441 1103 82-83 0.6581034546682716 1103 84-85 0.6582434218687467 1103 86-87 0.7805533803315043 1103 88-89 0.5726822818742612 1103 90-91 0.539622316276354 1103 92-93 0.9474313490368367 1103 94-95 0.9738574468841819 1103 96-97 1.0633933324692784 1103 98-99 1.1938895928241315 1103 100-101 1.7642111202282003 1104 1 0.11544525400282879 1104 2 0.1701226658201307 1104 3 0.09091529636123852 1104 4 0.06540729487006303 1104 5 0.10274267176679786 1104 6 0.05680856115291277 1104 7 0.12584089391072695 1104 8 0.10355172198730855 1104 9 0.12046284185709055 1104 10-11 0.16203191365784164 1104 12-13 0.13282273375361342 1104 14-15 0.1834136594758604 1104 16-17 0.1423310275036087 1104 18-19 0.0672869909862257 1104 20-21 0.06135038552678651 1104 22-23 0.09941594238410545 1104 24-25 0.13507808193142523 1104 26-27 0.1090135908317933 1104 28-29 0.13431394381650108 1104 30-31 0.13028568616458358 1104 32-33 0.007467889263814698 1104 34-35 0.053907562597096614 1104 36-37 -0.12259822857837577 1104 38-39 0.03340649536474416 1104 40-41 0.11009602231231241 1104 42-43 0.21155101855050162 1104 44-45 0.17480329186011545 1104 46-47 0.16361710576245514 1104 48-49 -0.08494185734800652 1104 50-51 -0.040642007904232 1104 52-53 -0.013921543001899295 1104 54-55 0.0046239574415167795 1104 56-57 0.0460101200124754 1104 58-59 0.08359536056070738 1104 60-61 -0.07686844815584237 1104 62-63 -0.11205774111888189 1104 64-65 -0.0039143202775093755 1104 66-67 0.10511338009337834 1104 68-69 0.1297774972839747 1104 70-71 0.07137962145401389 1104 72-73 0.2163407496854859 1104 74-75 0.29685673056958706 1104 76-77 0.21424159009500343 1104 78-79 0.14335721445829108 1104 80-81 0.06632568534915606 1104 82-83 0.11839379499408764 1104 84-85 0.06342064511849443 1104 86-87 0.20659196531766355 1104 88-89 0.14635976717965704 1104 90-91 0.2223552317644959 1104 92-93 0.35408431373079097 1104 94-95 0.5100726703678298 1104 96-97 0.8418705509543791 1104 98-99 0.9970383754565546 1104 100-101 1.3701320617226536 1105 1 0.10389592062695385 1105 2 0.0638913819610778 1105 3 0.039115818377339906 1105 4 0.06819787305872893 1105 5 0.10849970844812873 1105 6 0.07144482473326264 1105 7 0.03447632300409964 1105 8 0.03991747242533705 1105 9 0.12546094873019342 1105 10-11 0.1422124281350179 1105 12-13 0.05542196760390539 1105 14-15 0.024560163991239392 1105 16-17 0.16632861471126859 1105 18-19 0.28409829366741235 1105 20-21 0.21701683755511425 1105 22-23 0.11185915907116595 1105 24-25 0.05791532087090445 1105 26-27 0.07843140603742427 1105 28-29 0.05185777327221075 1105 30-31 -0.015422540584168587 1105 32-33 -0.06792536482403477 1105 34-35 0.09247656014652961 1105 36-37 0.0401214853789611 1105 38-39 0.08726469638207135 1105 40-41 0.15955868489665193 1105 42-43 0.27057900912432586 1105 44-45 0.3144371418086038 1105 46-47 0.40197227651746914 1105 48-49 0.3557466028052403 1105 50-51 0.2367915416625337 1105 52-53 0.37643282852614135 1105 54-55 0.3134277950741975 1105 56-57 0.14238727958334607 1105 58-59 0.38247119578227284 1105 60-61 0.42620819787811826 1105 62-63 0.30155543638758076 1105 64-65 0.2330862783031904 1105 66-67 0.15812932853315687 1105 68-69 0.3102695171874146 1105 70-71 0.1683171633777505 1105 72-73 0.10392783981931686 1105 74-75 0.19133127661790894 1105 76-77 0.09186143006491321 1105 78-79 0.04791381559450514 1105 80-81 0.29636788822687876 1105 82-83 -0.040841893848046595 1105 84-85 0.1320540509587289 1105 86-87 0.3191454954746291 1105 88-89 0.31491690225320923 1105 90-91 0.3498781066980676 1105 92-93 0.3751834438998465 1105 94-95 0.5408601188105564 1105 96-97 0.7933561308682577 1105 98-99 1.0021017553303082 1105 100-101 1.2546339007002452 1106 1 -0.13757756466812054 1106 2 -0.035799604795379025 1106 3 -0.05240462077104269 1106 4 -0.025119509430908238 1106 5 0.09857591467572036 1106 6 0.03515761720547772 1106 7 0.06775419665000015 1106 8 0.03174527586718767 1106 9 -0.22904394164348219 1106 10-11 -0.1876827068851341 1106 12-13 -0.2320669122890564 1106 14-15 -0.13482157866866373 1106 16-17 -0.08553098873272091 1106 18-19 -0.07405263143479601 1106 20-21 -0.07117202164351255 1106 22-23 -0.03207604378679463 1106 24-25 0.065233567410381 1106 26-27 0.09720903110410006 1106 28-29 0.11396135536890739 1106 30-31 0.03302587984534 1106 32-33 -0.06569742715765159 1106 34-35 -0.2909224701059401 1106 36-37 0.028749707324351448 1106 38-39 0.04449113762627377 1106 40-41 -0.06338822798024779 1106 42-43 -0.22492471626612343 1106 44-45 -0.10990799828206832 1106 46-47 -0.011154231756549393 1106 48-49 0.033003994842296436 1106 50-51 -0.02987913744930637 1106 52-53 -0.09827124677151033 1106 54-55 -0.1372310841509332 1106 56-57 -0.18593393259283175 1106 58-59 -0.011582319243373718 1106 60-61 -0.06949649330238827 1106 62-63 -0.1108428543107749 1106 64-65 -0.35020672445961765 1106 66-67 -0.26550305288878207 1106 68-69 -0.13280707736791442 1106 70-71 -0.0608485585779448 1106 72-73 -0.08880659759601883 1106 74-75 -0.08248718881968742 1106 76-77 -0.15642283738489127 1106 78-79 0.11216371232900713 1106 80-81 -0.12982448818225123 1106 82-83 -0.15504796973437962 1106 84-85 0.10917692989363914 1106 86-87 0.315233979784459 1106 88-89 -0.006058237351023621 1106 90-91 -0.01860761298206981 1106 92-93 0.0834621214089104 1106 94-95 0.3211705006826584 1106 96-97 0.25631555927620653 1106 98-99 0.07344770258204392 1106 100-101 0.5596218509975905 1107 1 0.13807889155171438 1107 2 0.1255907231864981 1107 3 0.014106952163061237 1107 4 0.08167848131074606 1107 5 0.0568026456911781 1107 6 0.00347870456882049 1107 7 0.017147282437449007 1107 8 0.15430530738496628 1107 9 0.15814211173974968 1107 10-11 0.09213882369695625 1107 12-13 0.1828305564394057 1107 14-15 0.10540062166621311 1107 16-17 0.0729516272428512 1107 18-19 0.22443431823524662 1107 20-21 0.11258115475755659 1107 22-23 0.12101959297081066 1107 24-25 0.07142228010613394 1107 26-27 0.08682845393479255 1107 28-29 0.08973245750620151 1107 30-31 0.07929220506940737 1107 32-33 0.11536097922469679 1107 34-35 0.1121970019712819 1107 36-37 0.12436656933557089 1107 38-39 0.1383426100974674 1107 40-41 0.14605430132143482 1107 42-43 0.23007774606028164 1107 44-45 0.13836522145594188 1107 46-47 0.002016062737681068 1107 48-49 0.14405944643295499 1107 50-51 0.05413504555078674 1107 52-53 0.22603152088336742 1107 54-55 0.17039188442978315 1107 56-57 0.21272459198118554 1107 58-59 0.3928756734681471 1107 60-61 0.23862307726788146 1107 62-63 0.2616606421927159 1107 64-65 0.14949897828832803 1107 66-67 0.3318461049955914 1107 68-69 0.3556940553022301 1107 70-71 0.5974526331880412 1107 72-73 0.499675821393442 1107 74-75 0.5263247879359518 1107 76-77 0.2915166878785769 1107 78-79 0.4306010214987097 1107 80-81 0.6854756201601084 1107 82-83 0.6575463373669699 1107 84-85 0.723824817217583 1107 86-87 0.7866050031273133 1107 88-89 0.8158982287181153 1107 90-91 1.0008949970839218 1107 92-93 1.0537375079941569 1107 94-95 1.0905576768867355 1107 96-97 0.9319044492594628 1107 98-99 0.9073564980119642 1107 100-101 1.3704058557252594 1108 1 0.1197615838594075 1108 2 0.04224456934034038 1108 3 0.10481849062460213 1108 4 0.0611977120799736 1108 5 0.032350722614260974 1108 6 0.03470947379958744 1108 7 0.08927228243745589 1108 8 0.08804569200034962 1108 9 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-0.6248459121504766 2316 76-77 -0.4543087226659388 2316 78-79 -0.43006103554537134 2316 80-81 -0.3440494446627085 2316 82-83 -0.5637198683374365 2316 84-85 -0.5305010254790439 2316 86-87 -0.41406591303517715 2316 88-89 -0.19196132184368153 2316 90-91 -0.33745526220734945 2316 92-93 -0.25771581957714673 2316 94-95 -0.18732974748664333 2316 96-97 -0.20374896474226745 2316 98-99 -0.19546891253254373 2316 100-101 0.5530138937546454 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 8.0 5 21.0 6 60.0 7 147.0 8 368.0 9 747.0 10 1365.0 11 2333.0 12 3761.0 13 5509.0 14 7114.0 15 10665.0 16 15026.0 17 22295.0 18 20439.0 19 10795.0 20 8123.0 21 8001.0 22 8354.0 23 8668.0 24 9785.0 25 11562.0 26 13669.0 27 16808.0 28 21203.0 29 26773.0 30 33264.0 31 42298.0 32 54430.0 33 71887.0 34 99025.0 35 144481.0 36 219438.0 37 336287.0 38 272091.0 39 24676.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.472020987362384 20.151931048989237 15.853075398510347 20.522972565138037 2 15.431216862120303 21.372006163972106 40.63468017865072 22.562096795256874 3 19.505184527385275 26.09064434403322 30.670005484890435 23.73416564369107 4 12.310452836767595 17.654110830119123 38.018477563192185 32.016958769921104 5 12.538226299694188 38.9293436275787 34.04715213659139 14.485277936135713 6 29.951093060307677 37.83320709379163 17.32846658134615 14.887233264554547 7 27.11200929819521 34.76800219395617 20.45132812704051 17.668660380808106 8 26.54073184109489 35.14867970851725 19.2178154464962 19.09277300389166 9 26.69894481155484 16.87302478648105 20.78466581346149 35.64336458850262 10-11 24.67805652048998 28.041567633922742 26.389668817091966 20.89070702849531 12-13 26.165243065679373 25.577899504795166 27.71844338447544 20.538414045050025 14-15 22.98084206127406 26.202431634758533 27.52265782119257 23.294068482774833 16-17 20.472157651422155 29.909238122600364 28.101770836054012 21.516833389923473 18-19 20.438987123566747 27.327748321884716 32.12278319011675 20.11048136443179 20-21 21.66923771325403 25.973969581662153 32.54824181223688 19.80855089284694 22-23 21.175624029112107 26.12276920244029 32.44637956354039 20.255227204907218 24-25 20.785024943192205 26.35786951184475 32.75348029357223 20.10362525139081 26-27 20.54660661966598 26.487870780108224 32.783794884251506 20.18172771597429 28-29 20.40238178569936 26.779025972669146 32.751708178360985 20.06688406327051 30-31 20.60604774466529 26.42316582652075 32.73705827043122 20.233728158382743 32-33 20.551133544022775 26.520914409590723 32.50026118525871 20.427690861127797 34-35 20.90432131010526 26.48829890041006 32.48305560634158 20.124324183143102 36-37 20.971048882190928 26.37066937951443 32.40141223053808 20.25686950775656 38-39 20.693838639747174 26.63439940449761 32.47473032622039 20.197031629534827 40-41 20.828010318133753 26.588587433646637 32.41129405342615 20.172108194793463 42-43 20.659800628216722 26.653134438312925 32.02563155569358 20.661433377776774 44-45 20.89220884373286 26.787160132682114 31.720002873037846 20.600628150547184 46-47 21.200178911902213 27.188275393736777 30.95420769451772 20.65733799984329 48-49 21.15708334421605 27.310934520855646 30.644409329537442 20.887572805390864 50-51 21.262961318463187 27.671827252069892 30.312279624937972 20.752931804528952 52-53 21.367115916341028 27.99887298524074 29.784238052158063 20.84977304626017 54-55 21.231455846632016 27.850739154282135 29.796732572413614 21.12107242667224 56-57 21.448402852143026 28.059981194661372 29.62820278423486 20.863413168960744 58-59 21.596625486457544 27.611069031263874 29.87965889205213 20.912646590226448 60-61 21.297045994724055 27.77770522631703 30.260760832658605 20.664487946300312 62-63 21.204684357614855 27.700557630527335 30.354558988690677 20.740199023167133 64-65 21.25120798182151 27.682731736620784 30.263274740773632 20.80278554078408 66-67 21.16410269804372 27.874964087026928 29.85651134849949 21.10442186642986 68-69 21.120778593256194 27.861970120406404 29.887233264554546 21.130018021782853 70-71 21.309778776085878 27.81871131193355 29.805221093321492 21.066288818659075 72-73 21.29861310627628 27.73702562227388 29.72702875649698 21.237332514952854 74-75 21.133348133831326 28.370367226473736 29.208771906913572 21.287512732781362 76-77 21.24118499751874 27.892202314101393 29.65037088306736 21.216241805312507 78-79 21.27742445216392 27.967227779664118 29.461566589181707 21.29378117899026 80-81 21.243241831431035 28.354402277535456 29.22225559589417 21.180100295139344 82-83 21.216719660119292 28.210778016145838 29.35068954608243 21.221812777652445 84-85 21.16821136183478 28.191035065472704 29.2362345394449 21.40451903324762 86-87 21.07291639459869 28.133537493143884 29.288368114503616 21.50517799775381 88-89 21.216274453469847 28.020933998485127 29.189019771724084 21.573771776320942 90-91 21.247138500961423 27.975640647636695 29.240397917746208 21.53682293365567 92-93 21.240675086364313 27.875145654587797 29.34062098725488 21.54355827179301 94-95 21.062207798991825 27.913554209000445 29.320395956852195 21.703842035155535 96-97 20.965112179068612 28.01466555227623 29.315825214824876 21.70439705383028 98-99 20.97701299532346 27.959632354189495 29.028406620027503 22.034948030459546 100-101 20.841048775526264 27.881482971389165 29.43771936154505 21.83974889153952 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 879.0 1 623.0 2 525.5 3 716.0 4 569.5 5 478.5 6 992.0 7 2435.5 8 4101.5 9 4860.0 10 4367.0 11 3614.0 12 3573.5 13 3799.5 14 3987.5 15 4050.0 16 4125.5 17 4343.5 18 4509.0 19 4656.5 20 4952.5 21 5536.0 22 6248.5 23 7270.0 24 8475.0 25 9860.5 26 12258.0 27 15222.0 28 17251.5 29 19466.5 30 22363.5 31 24641.5 32 27580.5 33 30952.5 34 34578.5 35 37857.0 36 42026.5 37 45912.5 38 47524.0 39 48890.5 40 49826.5 41 51059.0 42 51464.0 43 52627.0 44 54875.5 45 55945.0 46 56188.5 47 58183.5 48 63573.0 49 61383.5 50 53536.5 51 48361.0 52 44285.5 53 42188.5 54 40487.5 55 37076.0 56 32416.5 57 28455.5 58 25184.5 59 22361.5 60 18966.5 61 15068.0 62 11652.5 63 8452.0 64 6071.5 65 4548.0 66 3283.0 67 2423.0 68 1709.5 69 1102.0 70 767.5 71 559.5 72 451.0 73 385.0 74 289.0 75 236.5 76 170.5 77 82.5 78 54.0 79 37.0 80 23.0 81 13.0 82 11.0 83 4.5 84 2.0 85 1.5 86 0.5 87 0.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0427037897981038 2 0.0 3 0.0 4 6.529631467599969E-5 5 0.009076187739963956 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0061052054222059705 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.632407866899992E-4 22-23 0.0013712226081959934 24-25 0.0 26-27 8.488520907879958E-4 28-29 0.0015671115522239926 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.3059262935199937E-4 38-39 0.0 40-41 9.794447201399954E-5 42-43 0.0209274688536579 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.2648157337999844E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 9.794447201399954E-5 84-85 1.3059262935199937E-4 86-87 0.0 88-89 0.0 90-91 0.010349465876145951 92-93 0.00205683391229399 94-95 0.0 96-97 0.0 98-99 3.9177788805599815E-4 100-101 0.012373651631101941 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1531480.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.57841728903539 #Duplication Level Percentage of deduplicated Percentage of total 1 74.48376176586777 39.16238307382895 2 14.166769121909024 14.897325970583083 3 4.888565929712412 7.710991781921413 4 2.1699203456916893 4.5636390967898235 5 1.1541265017968805 3.0341072407905516 6 0.6950490452486158 2.1926747242456415 7 0.4629866210907255 1.7040172634064075 8 0.3242833963364162 1.3640246185985394 9 0.22894319744707886 1.083372387377268 >10 1.2779533357142019 12.338988581567085 >50 0.09616099048130981 3.4559864476751807 >100 0.04761574618388203 4.419657746799435 >500 0.0027421953367638588 1.0320031989647527 >1k 7.478714554810524E-4 0.736368066524923 >5k 1.246452425801754E-4 0.5662353234937109 >10k+ 2.492904851603508E-4 1.7382244774332978 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14250 0.9304724841329955 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12273 0.8013816700185441 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8640 0.5641601588006373 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2919 0.1905999425392431 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2918 0.1905346462245671 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2099 0.13705696450492336 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.2442604539399796E-4 0.0 24-25 0.0 0.0 0.0 9.141484054639955E-4 0.0 26-27 0.0 0.0 0.0 0.001273278136181994 0.0 28-29 0.0 0.0 0.0 0.002285371013659989 0.0 30-31 0.0 0.0 0.0 0.004342204925953979 0.0 32-33 0.0 0.0 0.0 0.007574372502415963 0.0 34-35 0.0 0.0 0.0 0.009990336145427953 0.0 36-37 0.0 0.0 0.0 0.013548985295269936 0.0 38-39 0.0 0.0 0.0 0.019490949930785907 0.0 40-41 0.0 0.0 0.0 0.025792044297019874 0.0 42-43 0.0 0.0 0.0 0.03173400893253585 0.0 44-45 0.0 0.0 0.0 0.03467234309295583 0.0 46-47 0.0 0.0 0.0 0.039471622221641814 0.0 48-49 0.0 0.0 0.0 0.04518504975579178 0.0 50-51 0.0 0.0 0.0 0.05230234805547575 0.0 52-53 0.0 0.0 0.0 0.0655248517773657 0.0 54-55 0.0 0.0 0.0 0.07398072452790765 0.0 56-57 0.0 0.0 0.0 0.09725886070990153 0.0 58-59 0.0 0.0 0.0 0.13607751978478333 0.0 60-61 0.0 0.0 0.0 0.1708478073497532 0.0 62-63 0.0 0.0 0.0 0.19396270274505706 0.0 64-65 0.0 0.0 0.0 0.2326834173479249 0.0 66-67 0.0 0.0 0.0 0.2987306396426986 0.0 68-69 0.0 0.0 0.0 0.3879254054901141 0.0 70-71 0.0 0.0 0.0 0.4751612818972497 0.0 72-73 0.0 0.0 0.0 0.5695797529187453 0.0 74-75 0.0 0.0 0.0 0.6818241178467888 0.0 76-77 0.0 0.0 0.0 0.7668399195549402 0.0 78-79 0.0 0.0 0.0 0.8741217645676078 0.0 80-81 0.0 0.0 0.0 1.015129156110429 0.0 82-83 0.0 0.0 0.0 1.1449382296863164 0.0 84-85 0.0 0.0 0.0 1.2976663097134797 0.0 86-87 0.0 0.0 0.0 1.496591532373913 0.0 88-89 0.0 0.0 0.0 1.7439666205239377 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10650 0.0 32.62176 1 GTATACG 80 4.479443E-4 29.704325 1 GTATCAA 15345 0.0 28.649334 1 GTATAGG 560 0.0 24.612154 1 GAGTACT 10795 0.0 24.467705 12-13 TACATGG 9530 0.0 24.226152 2 GTACATG 9820 0.0 24.102245 1 GTACCGG 120 1.704617E-4 23.763458 1 ATCAACG 18355 0.0 23.759066 3 TATCAAC 18440 0.0 23.649548 2 TCAACGC 18510 0.0 23.534445 4 CAACGCA 18585 0.0 23.465034 5 AACGCAG 19005 0.0 22.992706 6 GTACTTT 11520 0.0 22.738573 14-15 ACATGGG 9765 0.0 22.28098 3 AGTACTT 11140 0.0 21.812304 12-13 ACCGTGC 135 3.7939395E-4 21.113417 8 CATGGGA 6720 0.0 20.924906 4 CCCGCGA 125 9.509677E-9 20.898872 20-21 CGCAGAG 21075 0.0 20.647572 8 >>END_MODULE