FastQCFastQC Report
Thu 26 May 2016
SRR937628_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937628_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434765
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104640.729318041630511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82240.5731949134527258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57060.3976957899028761No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22520.15695950207873763No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19810.1380713914822288No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19280.1343774067530223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120150.031.6300581
GGTATCA80300.030.0309721
TATCAAC145250.026.0195792
ATCAACG146000.025.8841083
TCAACGC146950.025.749124
CAACGCA147900.025.6149735
AACGCAG150450.025.321986
GTACATG97900.024.4863951
TACATGG97800.024.1583232
GAGTACT76950.023.4847412-13
AGTACTT81450.023.3534512-13
ACATGGG98600.022.8035873
GTACCTG11750.022.6687281
TACCTGG10700.022.6587332
ACGCAGA169200.022.403617
CGCAGAG170050.022.2357588
CATGGGA69150.021.72274
ATGGGAT28250.021.705765
GTACTTT83400.021.66847214-15
GCAGAGT172350.021.2753149
Produced by FastQC (version 0.11.5)