##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937628_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1434765 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.660115071109207 33.0 31.0 34.0 30.0 34.0 2 31.738385031695085 33.0 31.0 34.0 30.0 34.0 3 31.768002425484312 33.0 31.0 34.0 30.0 34.0 4 35.40471645182312 37.0 35.0 37.0 33.0 37.0 5 35.425682951563495 37.0 35.0 37.0 33.0 37.0 6 35.49768847163124 37.0 35.0 37.0 33.0 37.0 7 35.41994891149422 37.0 35.0 37.0 33.0 37.0 8 35.43045097977718 37.0 35.0 37.0 33.0 37.0 9 36.96910643903357 39.0 37.0 39.0 33.0 39.0 10-11 36.88236505629842 39.0 37.0 39.0 33.0 39.0 12-13 36.77114788833015 39.0 37.0 39.0 33.0 39.0 14-15 37.82402274937011 40.0 37.5 41.0 33.0 41.0 16-17 37.73830662164187 40.0 37.5 41.0 33.0 41.0 18-19 37.71786006767658 40.0 37.5 41.0 32.0 41.0 20-21 37.76219903607908 40.0 37.5 41.0 32.5 41.0 22-23 37.76676424362178 40.0 38.0 41.0 32.5 41.0 24-25 37.686513470847146 40.0 37.0 41.0 32.0 41.0 26-27 37.5785996312985 40.0 37.0 41.0 32.0 41.0 28-29 37.428513031750846 40.0 37.0 41.0 32.0 41.0 30-31 37.15663854359425 39.0 36.5 41.0 31.5 41.0 32-33 36.95827016967935 39.0 36.0 41.0 31.0 41.0 34-35 36.88727701052089 39.0 36.0 41.0 31.0 41.0 36-37 36.698984154199465 39.0 35.5 41.0 30.0 41.0 38-39 36.50898927698961 39.0 35.0 41.0 30.0 41.0 40-41 36.24850376194011 39.0 35.0 41.0 30.0 41.0 42-43 35.93164873690117 39.0 35.0 40.0 29.0 41.0 44-45 35.59747066592787 39.0 35.0 40.0 27.5 41.0 46-47 35.12401543109847 38.5 35.0 40.0 25.0 41.0 48-49 34.675370531062576 38.0 34.0 40.0 24.0 41.0 50-51 33.7742208654379 37.5 33.5 39.5 22.5 40.5 52-53 33.88246367872091 38.0 33.5 39.5 23.0 40.5 54-55 34.13548211728053 38.0 34.0 40.0 22.0 41.0 56-57 33.88270204528268 38.0 33.5 40.0 20.5 41.0 58-59 33.59129474164759 38.0 33.0 40.0 18.5 41.0 60-61 33.22469463640387 37.5 33.0 40.0 17.0 41.0 62-63 32.84018323558213 37.0 32.0 40.0 13.5 41.0 64-65 32.39160663941482 36.0 31.5 39.0 8.0 41.0 66-67 31.872733513850697 36.0 31.0 39.0 7.5 40.0 68-69 31.37663101622914 35.0 30.5 38.5 4.0 40.0 70-71 30.887769774144196 35.0 30.0 38.0 2.0 40.0 72-73 30.392845169766478 35.0 29.5 37.5 2.0 39.5 74-75 29.820262900196198 34.0 29.0 37.0 2.0 39.0 76-77 29.25655873958453 34.0 28.5 36.0 2.0 39.0 78-79 28.70814279690402 34.0 27.5 36.0 2.0 38.0 80-81 28.118425317037982 34.0 26.5 35.0 2.0 37.0 82-83 27.534741577889065 33.0 26.0 35.0 2.0 37.0 84-85 26.947220624980396 33.0 25.5 35.0 2.0 36.0 86-87 26.335685286440636 32.0 24.5 35.0 2.0 36.0 88-89 25.735856394601207 32.0 22.0 35.0 2.0 35.0 90-91 25.111369806205197 31.5 19.0 34.0 2.0 35.0 92-93 24.390312699292217 31.0 14.5 34.0 2.0 35.0 94-95 23.564543670914748 31.0 4.5 34.0 2.0 35.0 96-97 22.60947367687391 30.5 2.0 34.0 2.0 35.0 98-99 21.373183413311587 29.5 2.0 34.0 2.0 35.0 100-101 18.208202388544464 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.324049255640233 1101 2 0.2621870205707033 1101 3 0.2849128738335587 1101 4 0.2592331271849062 1101 5 0.15606115372910523 1101 6 0.2925190290248807 1101 7 0.29482798731847737 1101 8 0.3054374547173424 1101 9 0.46531776500091837 1101 10-11 0.5337514320985051 1101 12-13 0.47444306825776295 1101 14-15 0.59395135991236 1101 16-17 0.6238535211159828 1101 18-19 0.7779787004899248 1101 20-21 0.6788502033431101 1101 22-23 0.6994218883637231 1101 24-25 0.7453661658664501 1101 26-27 0.7807473006226928 1101 28-29 0.6464990157933315 1101 30-31 0.5861367662813777 1101 32-33 0.795004492579281 1101 34-35 0.8239377179919813 1101 36-37 0.9973644508295223 1101 38-39 0.9097730913715338 1101 40-41 0.8844345060800904 1101 42-43 0.8216107738560368 1101 44-45 0.9579264466449544 1101 46-47 1.0754440922670128 1101 48-49 1.2476011369576057 1101 50-51 1.1559714689324139 1101 52-53 1.283200661944477 1101 54-55 1.354864126915487 1101 56-57 1.4063675174023302 1101 58-59 1.4099408698667162 1101 60-61 1.4434166958448031 1101 62-63 1.4990354309180205 1101 64-65 1.525518629982166 1101 66-67 1.529190755429287 1101 68-69 1.5901610360871636 1101 70-71 1.4889066759021894 1101 72-73 1.6712729068257985 1101 74-75 1.702653797343558 1101 76-77 1.63721641017003 1101 78-79 1.8467875850815147 1101 80-81 2.019862946810317 1101 82-83 2.114119974894148 1101 84-85 2.308844345904859 1101 86-87 2.6627242533967497 1101 88-89 2.604030526853723 1101 90-91 2.736463216066152 1101 92-93 2.865087115065613 1101 94-95 3.1258024837129312 1101 96-97 3.2072172773273984 1101 98-99 3.7684636817899317 1101 100-101 4.301292581483391 1102 1 0.16003341872865562 1102 2 0.20154787186142542 1102 3 0.09773631004253502 1102 4 0.09761145088487666 1102 5 0.13393939329075977 1102 6 0.1794919591749462 1102 7 0.2325311246378874 1102 8 0.10682895126388559 1102 9 0.31457923853704983 1102 10-11 0.24931191620581217 1102 12-13 0.30481927053763513 1102 14-15 0.36392664782612627 1102 16-17 0.36916824570005957 1102 18-19 0.47969961273837924 1102 20-21 0.45681295937412614 1102 22-23 0.33901752338114477 1102 24-25 0.29399007892226336 1102 26-27 0.4338086119325837 1102 28-29 0.30924025170016733 1102 30-31 0.4240095510328672 1102 32-33 0.36364079949318295 1102 34-35 0.4155014806576389 1102 36-37 0.46767945902591634 1102 38-39 0.6748411772342209 1102 40-41 0.583068770985335 1102 42-43 0.7028554443079784 1102 44-45 0.7162819017387321 1102 46-47 0.6947361581180118 1102 48-49 0.7458195789428146 1102 50-51 0.4382713662647575 1102 52-53 0.4995139004000393 1102 54-55 0.6924814242414072 1102 56-57 0.7119739656118895 1102 58-59 0.803433101725453 1102 60-61 0.9624336104831741 1102 62-63 0.8450623060208287 1102 64-65 0.7898956471747454 1102 66-67 0.8658877307985016 1102 68-69 0.9728365306597659 1102 70-71 0.9181092431608526 1102 72-73 0.9016838043453248 1102 74-75 0.9383557189115166 1102 76-77 0.8610347394666178 1102 78-79 1.214765816891095 1102 80-81 1.4160208980705953 1102 82-83 1.727602242506947 1102 84-85 1.6657172648995058 1102 86-87 1.8571764183986126 1102 88-89 2.019683279500299 1102 90-91 2.0894461558401325 1102 92-93 2.019689150267574 1102 94-95 2.12031883963504 1102 96-97 2.352939410999557 1102 98-99 2.4920435403997416 1102 100-101 3.0011397617152333 1103 1 0.2363299811912256 1103 2 0.13609093091041302 1103 3 0.28775906725896405 1103 4 0.21999137662448476 1103 5 0.2600622822594971 1103 6 0.26590949420908316 1103 7 0.2459159611452506 1103 8 0.18147860990563913 1103 9 0.24861325458687844 1103 10-11 0.2957246602470178 1103 12-13 0.435433715381329 1103 14-15 0.49879309889811196 1103 16-17 0.5578435361540031 1103 18-19 0.4365657044859006 1103 20-21 0.48079786774639643 1103 22-23 0.355711139383061 1103 24-25 0.3602255562249326 1103 26-27 0.3566436820008576 1103 28-29 0.2979065590423673 1103 30-31 0.33197757115503634 1103 32-33 0.4493846011460789 1103 34-35 0.39897255503780116 1103 36-37 0.46643679523385373 1103 38-39 0.5455292835942629 1103 40-41 0.42818495256396716 1103 42-43 0.5531518869957281 1103 44-45 0.6347302189024546 1103 46-47 0.630986008399482 1103 48-49 0.7338720403154397 1103 50-51 0.6868190965318561 1103 52-53 0.6707769259120795 1103 54-55 0.7671909702948199 1103 56-57 0.8693880271761429 1103 58-59 0.9628533915805306 1103 60-61 1.1509442105550391 1103 62-63 1.208219570363866 1103 64-65 0.9340296752020549 1103 66-67 1.2284760647307031 1103 68-69 1.2596822606873097 1103 70-71 1.317453476003113 1103 72-73 1.2798428653107123 1103 74-75 1.455079278619266 1103 76-77 1.4991351107685702 1103 78-79 1.57859681461537 1103 80-81 1.6854094475974861 1103 82-83 1.948856284907233 1103 84-85 2.0698524906750535 1103 86-87 2.093881413008745 1103 88-89 2.2067715535450922 1103 90-91 2.292075213212275 1103 92-93 2.4300526668563904 1103 94-95 2.621546531574193 1103 96-97 2.6988640726356827 1103 98-99 2.806617142028678 1103 100-101 3.494986330166544 1104 1 0.3105774086857629 1104 2 0.24584643865711442 1104 3 0.33402497343131543 1104 4 0.3207502031950895 1104 5 0.4017393723680698 1104 6 0.3003902400271983 1104 7 0.41368501102767397 1104 8 0.4534987563341488 1104 9 0.5442162298541362 1104 10-11 0.49542988530997434 1104 12-13 0.42939645783058467 1104 14-15 0.6678112940583034 1104 16-17 0.6803435778477791 1104 18-19 0.7548713531575331 1104 20-21 0.6784025607968758 1104 22-23 0.6317355552533783 1104 24-25 0.5233995359784345 1104 26-27 0.4595905974959251 1104 28-29 0.5272668932596005 1104 30-31 0.56882944153746 1104 32-33 0.5636538761748398 1104 34-35 0.7920488879875478 1104 36-37 0.5384556908493252 1104 38-39 0.7333680454561389 1104 40-41 0.8842718256981712 1104 42-43 0.9179686011932873 1104 44-45 0.7764389962744147 1104 46-47 0.8782813336942539 1104 48-49 0.928889635085369 1104 50-51 0.7425369798226598 1104 52-53 0.8294438093863334 1104 54-55 0.9886490847358758 1104 56-57 1.0392676155752696 1104 58-59 1.2486574975827622 1104 60-61 1.2370439253696617 1104 62-63 1.2121979896697468 1104 64-65 1.3070784068776433 1104 66-67 1.4701106271963091 1104 68-69 1.435564500810063 1104 70-71 1.505759624999957 1104 72-73 1.4273533304463832 1104 74-75 1.4029170398307222 1104 76-77 1.6694875064924553 1104 78-79 1.7289470422633428 1104 80-81 1.9604040274076944 1104 82-83 1.9284897133023549 1104 84-85 2.0523790496050225 1104 86-87 2.2561573090078113 1104 88-89 2.3927749557565328 1104 90-91 2.3017364930441637 1104 92-93 2.5604282443150694 1104 94-95 2.862556521400915 1104 96-97 2.877800798173947 1104 98-99 3.3718853997295177 1104 100-101 3.8875068851939822 1105 1 0.08253776844592409 1105 2 0.15271685837729976 1105 3 0.0330922619056615 1105 4 0.014562661556183798 1105 5 -0.04017435181581419 1105 6 0.15505379765544802 1105 7 0.03452112028816856 1105 8 0.06753106812629284 1105 9 0.2779437448441442 1105 10-11 0.0662368473352899 1105 12-13 0.24865445250080853 1105 14-15 0.043456697122927324 1105 16-17 0.02458908084577871 1105 18-19 0.08243630985307249 1105 20-21 0.0956369045676908 1105 22-23 0.05920474246190821 1105 24-25 -0.12337344869844458 1105 26-27 0.017268812019580082 1105 28-29 0.17394410844947572 1105 30-31 0.09978796918569088 1105 32-33 0.15611307004559194 1105 34-35 0.20651840830733192 1105 36-37 0.3037413337540613 1105 38-39 0.18992200948012083 1105 40-41 0.23624134601796243 1105 42-43 0.15256636934785206 1105 44-45 0.28281807688734517 1105 46-47 0.35276501124256043 1105 48-49 0.274176110768245 1105 50-51 0.2366075994096093 1105 52-53 0.28392831971278554 1105 54-55 0.31869572031215654 1105 56-57 0.3723996941895251 1105 58-59 0.3335467380890904 1105 60-61 0.5653125796001746 1105 62-63 0.6134887957989932 1105 64-65 0.3826696793626567 1105 66-67 0.6112706871563383 1105 68-69 0.36843425430772925 1105 70-71 0.4096653545136171 1105 72-73 0.19580353031313535 1105 74-75 0.3896841950605676 1105 76-77 0.3321498982312967 1105 78-79 0.6967643307376932 1105 80-81 0.8709886376675229 1105 82-83 0.8907773548746434 1105 84-85 1.1380802488055544 1105 86-87 1.269473431592754 1105 88-89 1.3902474740776505 1105 90-91 1.1943622425444964 1105 92-93 1.2058271450479126 1105 94-95 1.2672658818272957 1105 96-97 1.5895096589334408 1105 98-99 1.755017478343774 1105 100-101 2.2390267053139006 1106 1 -0.13679351014870278 1106 2 -0.17345966870683327 1106 3 0.017363906511537408 1106 4 -0.04816784170927946 1106 5 -0.02495546102546342 1106 6 -0.05774437228832596 1106 7 -0.02597463635625985 1106 8 0.017312351959631656 1106 9 0.1028250007376883 1106 10-11 -0.43999656341454596 1106 12-13 -0.45890959073602033 1106 14-15 -0.45153051169207714 1106 16-17 -0.2344542432907062 1106 18-19 -0.05280274997300438 1106 20-21 0.01029066945436341 1106 22-23 -0.11240864942856632 1106 24-25 -0.3283028570733961 1106 26-27 -0.405682852144146 1106 28-29 -0.1713528391284953 1106 30-31 -0.03822476961219934 1106 32-33 -0.11912900203905963 1106 34-35 -0.048328926935212735 1106 36-37 -0.252784135110538 1106 38-39 -0.466994398161404 1106 40-41 -0.1969653123831847 1106 42-43 -0.0769775572403475 1106 44-45 -0.27685869466994717 1106 46-47 0.021946550026477496 1106 48-49 -0.17982539099545392 1106 50-51 -0.05039515964463703 1106 52-53 0.08222639361352435 1106 54-55 0.09593701532141097 1106 56-57 -0.34240861265921296 1106 58-59 -0.52503405155921 1106 60-61 -0.34731031181881633 1106 62-63 -0.5277132263432875 1106 64-65 -0.42289164847585425 1106 66-67 -0.22418687055679243 1106 68-69 -0.16328832104239055 1106 70-71 -0.23439874984149967 1106 72-73 -0.2932700473769394 1106 74-75 -0.20138860555410787 1106 76-77 0.015887849196968062 1106 78-79 0.19811875570189486 1106 80-81 0.2635832837053087 1106 82-83 0.19324238648152914 1106 84-85 0.21044037523310521 1106 86-87 0.35619111747967125 1106 88-89 0.3825454781289217 1106 90-91 0.6625997220262114 1106 92-93 0.8164331328941046 1106 94-95 0.798411980734155 1106 96-97 1.2444532554828776 1106 98-99 1.4390811024837546 1106 100-101 1.9188897566881877 1107 1 0.01082724213013364 1107 2 0.16126948995624701 1107 3 0.11203963032671638 1107 4 -0.005174180549076368 1107 5 0.12495722713155999 1107 6 0.17742221870807384 1107 7 0.136494804498696 1107 8 0.17542580496839832 1107 9 0.2779437448441442 1107 10-11 0.29419737365108034 1107 12-13 0.4081939261850138 1107 14-15 0.5033251181755602 1107 16-17 0.434128554529984 1107 18-19 0.5304626256425493 1107 20-21 0.49070269404138145 1107 22-23 0.43058632140927955 1107 24-25 0.4707054986699717 1107 26-27 0.4666109172827433 1107 28-29 0.4726283189757936 1107 30-31 0.4797221797120059 1107 32-33 0.5682841226771771 1107 34-35 0.5552026188336541 1107 36-37 0.5524255442803749 1107 38-39 0.5672246410590702 1107 40-41 0.5888729249653224 1107 42-43 0.5788821588215356 1107 44-45 0.7150549189925997 1107 46-47 0.4955281691372946 1107 48-49 0.522202426557719 1107 50-51 0.26489707309382027 1107 52-53 0.4684677933969965 1107 54-55 0.6436957203121594 1107 56-57 0.6536496941895322 1107 58-59 0.7164414749311945 1107 60-61 0.8001810006528132 1107 62-63 0.8411203747463603 1107 64-65 0.8237881004152854 1107 66-67 1.0112706871563368 1107 68-69 0.9240263595708846 1107 70-71 1.0067048281978295 1107 72-73 0.9882377408394554 1107 74-75 1.0929736687447829 1107 76-77 1.0972814771786652 1107 78-79 1.0790011728429576 1107 80-81 1.2755939008254131 1107 82-83 1.627619460137801 1107 84-85 1.7449881435423968 1107 86-87 1.7125655368559158 1107 88-89 1.6234711582881793 1107 90-91 1.8489675057023938 1107 92-93 2.1360903029426517 1107 94-95 2.5666079870904532 1107 96-97 2.5592465010387038 1107 98-99 2.9135701099227234 1107 100-101 3.330474073734951 1108 1 0.09363821518611815 1108 2 0.0390371850560669 1108 3 0.08581458495466521 1108 4 -0.04973127952995071 1108 5 0.11337688370003463 1108 6 0.08255624076587509 1108 7 0.2668713925204713 1108 8 0.12511343584931467 1108 9 0.2924383700012001 1108 10-11 0.2765650966721651 1108 12-13 0.35719923122480424 1108 14-15 0.28795533596139933 1108 16-17 0.2486484833307685 1108 18-19 0.35989687107043977 1108 20-21 0.26211306679022783 1108 22-23 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1082.0 5 1236.0 6 1430.0 7 1771.0 8 2159.0 9 2750.0 10 3440.0 11 4618.0 12 6430.0 13 8208.0 14 9552.0 15 12388.0 16 15954.0 17 21317.0 18 17863.0 19 12321.0 20 11843.0 21 12845.0 22 13436.0 23 15003.0 24 16713.0 25 19084.0 26 21975.0 27 25157.0 28 30093.0 29 35620.0 30 42807.0 31 52089.0 32 64433.0 33 82520.0 34 107668.0 35 147335.0 36 198165.0 37 244820.0 38 151998.0 39 11781.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.08177165390121 19.660482884636767 15.16603486218131 21.091710599280713 2 14.769321524026793 20.753304592880866 41.46781796222364 23.0095559208687 3 19.278810398528236 26.314983811712604 30.054785749833645 24.351420039925518 4 11.886933955109912 17.024698425079627 38.50268986866227 32.58567775114818 5 12.533898636785542 39.075692510707775 33.91177687567797 14.47863197682871 6 29.87267774828887 37.374144433580106 17.935465847185363 14.817711970945663 7 27.402699890356235 34.48380135623049 20.52251887339288 17.590979880020395 8 26.344575289022487 35.0362373752365 19.513496562739864 19.105690773001147 9 26.84319920883026 17.05512765713743 20.790555131847654 35.31111800218466 10-11 24.487972907000863 27.93494104311399 26.70286330090117 20.87422274898398 12-13 25.8560461637908 25.44033737226925 27.824865909120277 20.878750554819675 14-15 22.93358494756504 26.16167587929082 27.329349363730042 23.575389809414094 16-17 20.732602830225243 29.940943569036456 27.752891104382844 21.573562496355457 18-19 20.603776905194692 27.787969293703508 31.290681199062302 20.317572602039498 20-21 21.99748753368402 26.4802637321507 31.39238676618771 20.12986196797758 22-23 21.384412699377098 26.6414140091492 31.347479989398913 20.626693302074788 24-25 20.89374734411134 26.86863699706591 31.629036994412203 20.60857866441055 26-27 20.707130670171672 27.06296788017239 31.591702244009 20.63819920564693 28-29 20.594687580538032 27.285254617164544 31.57921938961165 20.540838412685776 30-31 20.777775798828905 27.054838652224067 31.563908582340396 20.603476966606635 32-33 20.62499497993896 27.097957716942595 31.459316901524037 20.817730401594407 34-35 21.01712684087435 27.11110148813505 31.32462632490046 20.54714534609014 36-37 21.002849221701574 26.958944670628576 31.311794241499474 20.72641186617038 38-39 20.77382039297482 27.250185307837953 31.35914876055853 20.6168455386287 40-41 20.910727976304358 27.23784041190871 31.254285478877392 20.597146132909543 42-43 20.68917838883052 27.183374132643674 31.006812454805644 21.120635023720162 44-45 20.934809957963196 27.3696986541868 30.770283656765436 20.925207731084576 46-47 21.134773368681223 27.681070046505845 30.086886804114638 21.097269780698294 48-49 21.111745137302368 27.781510539075143 29.933292218636133 21.173452104986357 50-51 21.212945554304614 28.12345475523323 29.671807471930673 20.991792218531483 52-53 21.25099840081248 28.44674852243665 29.17240736219973 21.12984571455114 54-55 21.116676997215116 28.1859498721049 29.321309156939947 21.376063973740038 56-57 21.347676807523865 28.430939592980938 29.051609626163167 21.169773973332028 58-59 21.441997209731245 28.08621560463211 29.36571124802222 21.10607593761442 60-61 21.298396083348926 28.132950810057505 29.726568936577845 20.842084170015724 62-63 21.15626489053656 28.129390799241094 29.759430018623533 20.954914291598815 64-65 21.17253757021013 28.14951070664148 29.623377997142196 21.054573726006193 66-67 21.119268553235166 28.31716972832576 29.2363307569315 21.327230961507578 68-69 20.97805888642853 28.36391018285367 29.311185670533852 21.34684526018395 70-71 21.27647244463932 28.300101100091883 29.18374941699878 21.239677038270017 72-73 21.146847912195494 28.229396117075 29.2174970785715 21.406258892158004 74-75 20.92749773307118 28.799999441342152 28.896333004772686 21.37616982081399 76-77 21.05531831688181 28.39694549873078 29.098628826419272 21.449107357968135 78-79 21.108209636596428 28.40693686342574 28.98915211652266 21.49570138345517 80-81 21.0391326176838 28.805010058802623 28.71374414400323 21.442113179510347 82-83 21.16856562245917 28.69418380858721 28.82377152624099 21.31347904271263 84-85 21.12507610073783 28.590069761336917 28.75825937365602 21.526594764269237 86-87 21.009411675169257 28.5541126810619 28.91174167484458 21.524733968924263 88-89 21.14739377231443 28.411432529682163 28.756034017438857 21.685139680564546 90-91 21.1658949846839 28.300991583999753 28.858892869686407 21.674220561629937 92-93 21.297421843634904 28.292656530922166 28.81481610780429 21.59510551763864 94-95 21.061072761060508 28.341612545441407 28.83843509450311 21.758879598994973 96-97 20.924973552732187 28.33897192574515 28.831327530645662 21.904726990877002 98-99 21.00163259947824 28.37119921344737 28.537950605835462 22.08921758123893 100-101 21.34632489182234 27.60715487522044 29.278061612744228 21.76845862021299 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2240.0 1 1336.5 2 509.0 3 597.0 4 545.0 5 527.0 6 932.0 7 2021.5 8 3268.5 9 3819.5 10 3388.0 11 2925.0 12 3007.5 13 3131.0 14 3320.5 15 3543.0 16 3592.5 17 3661.5 18 3857.0 19 3991.0 20 4238.5 21 4778.0 22 5535.0 23 6593.5 24 7704.5 25 9164.0 26 11463.5 27 14004.5 28 16028.0 29 18247.0 30 20759.0 31 23056.5 32 26017.5 33 29282.5 34 33065.0 35 36094.5 36 39602.0 37 43234.0 38 44772.0 39 46003.5 40 46809.0 41 47914.0 42 48708.5 43 49890.5 44 51589.0 45 52307.5 46 52711.5 47 54221.5 48 57985.5 49 56800.5 50 50567.0 51 45571.0 52 41755.0 53 39553.5 54 37787.0 55 34595.5 56 30703.0 57 27174.5 58 23968.0 59 21206.5 60 17990.0 61 14476.5 62 11165.5 63 8053.0 64 5999.5 65 4635.5 66 3338.5 67 2425.5 68 1722.5 69 1173.0 70 841.5 71 606.5 72 466.5 73 383.5 74 278.0 75 203.5 76 154.0 77 98.0 78 59.0 79 37.5 80 25.0 81 12.5 82 10.0 83 9.5 84 7.0 85 7.5 86 4.5 87 3.5 88 5.0 89 4.0 90 2.5 91 3.5 92 2.0 93 2.0 94 2.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32994950392573 2 0.2808125372447753 3 0.2858307806504898 4 0.28060344376953716 5 0.2808125372447753 6 0.20665405136032733 7 0.19898729060159678 8 0.20602677093461297 9 0.2063752600600098 10-11 0.20602677093461297 12-13 0.2086404393750893 14-15 0.20515554812112088 16-17 0.1972099960620729 18-19 0.1913902276679456 20-21 0.1886023146647709 22-23 0.19769788083762846 24-25 0.19553724826016802 26-27 0.2026812753308033 28-29 0.2082571013371528 30-31 0.21107986325286718 32-33 0.20975560457635922 34-35 0.2095465111011211 36-37 0.19480542109683469 38-39 0.18738260272588195 40-41 0.18142343868159594 42-43 0.19205235700619963 44-45 0.19553724826016802 46-47 0.2022630883803271 48-49 0.20895407958794648 50-51 0.20581767745937485 52-53 0.21609810665858173 54-55 0.204040382919851 56-57 0.1758127637627068 58-59 0.15936407704397584 60-61 0.17570821702508774 62-63 0.16988844863096048 64-65 0.17748551156461162 66-67 0.17668398657619888 68-69 0.17420971378588132 70-71 0.17570821702508774 72-73 0.18640683317477078 74-75 0.19247054395667582 76-77 0.19375995372064417 78-79 0.19058870267953287 80-81 0.1872083581631835 82-83 0.17637034636334173 84-85 0.17096876491969068 86-87 0.17804309416524658 88-89 0.18651137991238984 90-91 0.18205071910731024 92-93 0.17703247570159572 94-95 0.1786703745909609 96-97 0.18609319296191362 98-99 0.1876962429387391 100-101 0.1672050823654048 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1434765.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.87777945511775 #Duplication Level Percentage of deduplicated Percentage of total 1 76.38299399890329 42.681120927923 2 13.418672640819922 14.996112608083163 3 4.458838211510012 7.474499346264244 4 1.956185098497568 4.3722911802893965 5 1.0716566148847841 2.9940895989075016 6 0.602251606254871 2.0191489458480056 7 0.3843424218580092 1.5033340758679368 8 0.28070707012430823 1.2548230204718693 9 0.19071567701552442 0.959109168501625 >10 1.12711190568978 11.605444949733082 >50 0.0836616733760416 3.1801648975682926 >100 0.039863973875256475 3.851677065072118 >500 0.0018744419941796131 0.7055263251882813 >1k 7.497767976718452E-4 0.6993117722447186 >5k 2.499255992239484E-4 0.9726822753239072 >10k+ 1.249627996119742E-4 0.7306638427128044 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10464 0.729318041630511 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8224 0.5731949134527258 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5706 0.3976957899028761 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2252 0.15695950207873763 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1981 0.1380713914822288 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1928 0.1343774067530223 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 1.045467376190526E-4 0.0 26-27 0.0 0.0 0.0 4.530358630158946E-4 0.0 28-29 0.0 0.0 0.0 0.0011151652012698944 0.0 30-31 0.0 0.0 0.0 0.003275797778730315 0.0 32-33 0.0 0.0 0.0 0.005959164044285998 0.0 34-35 0.0 0.0 0.0 0.0076319118461908395 0.0 36-37 0.0 0.0 0.0 0.011081954187619574 0.0 38-39 0.0 0.0 0.0 0.01725021170714368 0.0 40-41 0.0 0.0 0.0 0.022791188800953467 0.0 42-43 0.0 0.0 0.0 0.02836701480730294 0.0 44-45 0.0 0.0 0.0 0.0308761365101602 0.0 46-47 0.0 0.0 0.0 0.03443072558920798 0.0 48-49 0.0 0.0 0.0 0.03944896899492252 0.0 50-51 0.0 0.0 0.0 0.04523388847651009 0.0 52-53 0.0 0.0 0.0 0.057431007865399564 0.0 54-55 0.0 0.0 0.0 0.06534171101190787 0.0 56-57 0.0 0.0 0.0 0.08618136071063903 0.0 58-59 0.0 0.0 0.0 0.1221802873641328 0.0 60-61 0.0 0.0 0.0 0.1552867542768328 0.0 62-63 0.0 0.0 0.0 0.17407031813572257 0.0 64-65 0.0 0.0 0.0 0.20728133178604163 0.0 66-67 0.0 0.0 0.0 0.2679184396050921 0.0 68-69 0.0 0.0 0.0 0.35765438939477895 0.0 70-71 0.0 0.0 0.0 0.4442190881433545 0.0 72-73 0.0 0.0 0.0 0.5335716998951048 0.0 74-75 0.0 0.0 0.0 0.6477018884625705 0.0 76-77 0.0 0.0 0.0 0.7303286600941618 0.0 78-79 0.0 0.0 0.0 0.8343178151125794 0.0 80-81 0.0 0.0 0.0 0.9718664729067129 0.0 82-83 0.0 0.0 0.0 1.1011210895164016 0.0 84-85 0.0 0.0 0.0 1.253027499276885 0.0 86-87 0.0 0.0 0.0 1.4520147898784819 0.0 88-89 0.0 0.0 0.0 1.7059936644677003 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12015 0.0 31.630058 1 GGTATCA 8030 0.0 30.030972 1 TATCAAC 14525 0.0 26.019579 2 ATCAACG 14600 0.0 25.884108 3 TCAACGC 14695 0.0 25.74912 4 CAACGCA 14790 0.0 25.614973 5 AACGCAG 15045 0.0 25.32198 6 GTACATG 9790 0.0 24.486395 1 TACATGG 9780 0.0 24.158323 2 GAGTACT 7695 0.0 23.48474 12-13 AGTACTT 8145 0.0 23.35345 12-13 ACATGGG 9860 0.0 22.803587 3 GTACCTG 1175 0.0 22.668728 1 TACCTGG 1070 0.0 22.658733 2 ACGCAGA 16920 0.0 22.40361 7 CGCAGAG 17005 0.0 22.235758 8 CATGGGA 6915 0.0 21.7227 4 ATGGGAT 2825 0.0 21.70576 5 GTACTTT 8340 0.0 21.668472 14-15 GCAGAGT 17235 0.0 21.275314 9 >>END_MODULE