FastQCFastQC Report
Thu 26 May 2016
SRR937628_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937628_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434765
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136180.949144981930839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117050.8158130425540071No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82890.5777252720828846No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27890.19438723414635845No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26140.182190114757469No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21640.15082609347175321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98550.031.8232541
CGTACAG953.4907684E-530.0008495
GGACCGA3050.029.5889386
TAGCGCA650.005843742329.2315945
GTATCAA137900.028.2902091
TACATGG90400.023.1201232
TACCTGG10500.023.072082
GTACATG91800.022.9825151
GAGTACT105000.022.869312-13
CCGTAAG1252.2505327E-422.8085941
AGGACCG3750.022.8006445
TATCAAC171550.022.760772
ATCAACG171450.022.7463423
TCAACGC172900.022.5281094
CAACGCA175250.022.226025
AGTACTT109100.021.90101412-13
ACATGGG91250.021.8636343
AACGCAG178550.021.8137136
GTACTTT111700.021.2826914-15
CCGTACA1353.7958554E-421.1117064
Produced by FastQC (version 0.11.5)