##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937628_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1434765 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2205538886159 33.0 31.0 34.0 31.0 34.0 2 32.44013270465895 34.0 31.0 34.0 31.0 34.0 3 32.53353266911306 34.0 31.0 34.0 31.0 34.0 4 36.15885040407314 37.0 35.0 37.0 35.0 37.0 5 36.01615944074465 37.0 35.0 37.0 35.0 37.0 6 36.12354218286618 37.0 35.0 37.0 35.0 37.0 7 36.08862217854492 37.0 35.0 37.0 35.0 37.0 8 36.09054374758236 37.0 35.0 37.0 35.0 37.0 9 37.74649158573007 39.0 37.0 39.0 35.0 39.0 10-11 37.70732768083972 39.0 37.0 39.0 35.0 39.0 12-13 37.626498416116924 39.0 37.0 39.0 35.0 39.0 14-15 38.81129627499974 40.0 38.0 41.0 35.0 41.0 16-17 38.79884963739707 40.0 38.0 41.0 35.0 41.0 18-19 38.85152725359205 40.0 38.0 41.0 35.0 41.0 20-21 38.80894188246856 40.0 38.0 41.0 35.0 41.0 22-23 38.66988113035933 40.0 38.0 41.0 34.5 41.0 24-25 38.61089655797291 40.0 38.0 41.0 34.0 41.0 26-27 38.525321219851335 40.0 38.0 41.0 34.0 41.0 28-29 38.412661655393045 40.0 38.0 41.0 34.0 41.0 30-31 38.39085878175172 40.0 38.0 41.0 34.0 41.0 32-33 38.24543810310399 40.0 37.0 41.0 34.0 41.0 34-35 38.08292438134468 40.0 37.0 41.0 34.0 41.0 36-37 37.89923471788063 40.0 37.0 41.0 33.0 41.0 38-39 37.74747362808544 40.0 37.0 41.0 33.0 41.0 40-41 37.54056901304395 40.0 37.0 41.0 33.0 41.0 42-43 37.28940174871843 40.0 36.5 41.0 33.0 41.0 44-45 36.89753304548132 40.0 36.0 41.0 31.5 41.0 46-47 36.401920523570055 40.0 36.0 41.0 30.5 41.0 48-49 35.95216498869153 39.0 36.0 41.0 29.5 41.0 50-51 35.657991029889914 39.0 35.0 41.0 28.0 41.0 52-53 35.42768258216502 39.0 35.0 40.5 27.0 41.0 54-55 35.22433813202859 39.0 35.0 40.0 27.0 41.0 56-57 34.95334915473963 38.5 35.0 40.0 26.0 41.0 58-59 34.707148557429264 38.0 34.5 40.0 26.0 41.0 60-61 34.37611525232355 38.0 34.0 40.0 25.0 41.0 62-63 34.02430641951818 37.5 34.0 40.0 23.5 41.0 64-65 33.63468338020512 37.0 33.5 40.0 22.5 41.0 66-67 33.20880039588364 36.5 33.0 39.0 21.5 40.5 68-69 32.75912257408008 36.0 32.5 39.0 20.0 40.0 70-71 32.347244322240925 35.5 32.0 38.5 18.5 40.0 72-73 31.906072074520914 35.0 31.5 38.0 18.0 40.0 74-75 31.420961272403495 35.0 31.0 37.0 14.0 39.0 76-77 30.184435081703274 34.0 29.5 36.0 12.0 38.5 78-79 30.733612473122776 34.0 31.0 36.0 9.0 38.5 80-81 30.524636090230807 34.0 31.0 36.0 7.0 37.5 82-83 30.1639982854335 34.0 30.5 35.0 4.5 37.0 84-85 29.758441277839925 34.0 30.0 35.0 2.0 36.5 86-87 29.345819698696303 34.0 29.5 35.0 2.0 36.0 88-89 28.910855436256114 34.0 29.0 35.0 2.0 36.0 90-91 28.399255627228154 34.0 29.0 35.0 2.0 35.0 92-93 27.971524256585575 33.5 28.0 35.0 2.0 35.0 94-95 27.518424620059733 33.0 27.0 35.0 2.0 35.0 96-97 26.98627510428537 33.0 26.0 35.0 2.0 35.0 98-99 26.201346561980536 33.0 25.0 34.5 2.0 35.0 100-101 23.826009137384865 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31965974569602906 1101 2 0.3276927719407041 1101 3 0.21277945291054579 1101 4 0.17300562759196225 1101 5 0.15270900241556262 1101 6 0.207846385772136 1101 7 0.21108053146820538 1101 8 0.15717840044800369 1101 9 0.37007035764078466 1101 10-11 0.2735233413447631 1101 12-13 0.3449357385959715 1101 14-15 0.3729037994995039 1101 16-17 0.19769796583397437 1101 18-19 0.37923465132117684 1101 20-21 0.41828642590570553 1101 22-23 0.33260821644712024 1101 24-25 0.22158963482952743 1101 26-27 0.2597834731882571 1101 28-29 0.5252995643386313 1101 30-31 0.38597738837907514 1101 32-33 0.5048743672813103 1101 34-35 0.39579918737464936 1101 36-37 0.5070266588373329 1101 38-39 0.6100205539709052 1101 40-41 0.5905992453311271 1101 42-43 0.5518970245641199 1101 44-45 0.6520063559028202 1101 46-47 0.3519323161575443 1101 48-49 0.6096345682745721 1101 50-51 0.6671204746094546 1101 52-53 0.7340675589389534 1101 54-55 0.7956962006901307 1101 56-57 0.8457279034135112 1101 58-59 0.7214300004443075 1101 60-61 1.0028821062974416 1101 62-63 0.9793362640141439 1101 64-65 0.7409706511195111 1101 66-67 0.8206859500847443 1101 68-69 0.6885514048391244 1101 70-71 0.7384957880727328 1101 72-73 0.6602916349606396 1101 74-75 0.584006313814875 1101 76-77 0.670873460980939 1101 78-79 0.6778283382635628 1101 80-81 0.796632531590781 1101 82-83 0.6577438046177484 1101 84-85 0.84451129318143 1101 86-87 0.8667953299199738 1101 88-89 1.0544628383587877 1101 90-91 1.0923442457029893 1101 92-93 1.2209741771367213 1101 94-95 1.3257940702136466 1101 96-97 1.561217173954887 1101 98-99 1.7187023965360382 1101 100-101 2.3424739174775233 1102 1 0.2374835506010058 1102 2 0.25590529588230737 1102 3 0.1670776920562318 1102 4 0.06944506407320006 1102 5 -0.07470647638820083 1102 6 0.03746487048827163 1102 7 0.052982265473886514 1102 8 0.1325400534161929 1102 9 0.19964251901241425 1102 10-11 0.07749570994244692 1102 12-13 0.14716625490273572 1102 14-15 0.1920822786190186 1102 16-17 0.15908556743808333 1102 18-19 0.24949548956573864 1102 20-21 0.22485384717020196 1102 22-23 0.22791674882903834 1102 24-25 0.14042499031864963 1102 26-27 0.19671707576421227 1102 28-29 -0.012132770490289602 1102 30-31 0.12828440438048005 1102 32-33 0.12650316225040825 1102 34-35 0.10980571234861003 1102 36-37 0.2014811872751423 1102 38-39 0.17826122170773573 1102 40-41 0.22554245669225992 1102 42-43 0.2722930986134102 1102 44-45 0.28252781495582013 1102 46-47 0.5547408816275521 1102 48-49 0.3940757385724183 1102 50-51 0.41109489466954585 1102 52-53 0.36415201868585 1102 54-55 0.37162559824085406 1102 56-57 0.4577632755412253 1102 58-59 0.434447663819185 1102 60-61 0.43194759427133533 1102 62-63 0.4174158966794792 1102 64-65 0.47165003496230895 1102 66-67 0.6496873416758291 1102 68-69 0.4701259636801751 1102 70-71 0.3966162703649232 1102 72-73 0.4065250270962544 1102 74-75 0.3885019471306208 1102 76-77 0.27332722744699467 1102 78-79 0.44751528699212884 1102 80-81 0.5515529688831542 1102 82-83 0.4985013992828087 1102 84-85 0.4641115510795615 1102 86-87 0.432364842352591 1102 88-89 0.3458258035066173 1102 90-91 0.4371125911654765 1102 92-93 0.8553834883180436 1102 94-95 0.6821527055468231 1102 96-97 0.9065402178334914 1102 98-99 0.9575866846029513 1102 100-101 1.6824428335679862 1103 1 0.26254643238326025 1103 2 0.2980959774010543 1103 3 0.19800954018175787 1103 4 0.1519998610548754 1103 5 -0.046679167728477466 1103 6 0.1376265664997689 1103 7 0.15074846501874362 1103 8 0.18949298191062525 1103 9 0.26758478754756965 1103 10-11 0.19981574827039594 1103 12-13 0.26751000864569363 1103 14-15 0.3662797873026946 1103 16-17 0.3533603308828077 1103 18-19 0.33928169150608056 1103 20-21 0.17002692178991907 1103 22-23 0.30273109785286323 1103 24-25 0.3871910700886829 1103 26-27 0.33065527195537214 1103 28-29 0.295583722502883 1103 30-31 0.30447316950204595 1103 32-33 0.2723439815101969 1103 34-35 0.34586691728332397 1103 36-37 0.36075175860104736 1103 38-39 0.2586750437223486 1103 40-41 0.3413647108544282 1103 42-43 0.5354383854741087 1103 44-45 0.7651754374255759 1103 46-47 0.6622537574210554 1103 48-49 0.5769569219476764 1103 50-51 0.4801899958792717 1103 52-53 0.43619657492013886 1103 54-55 0.35139862495064733 1103 56-57 0.41665056743246964 1103 58-59 0.42565918615719056 1103 60-61 0.3906701957805012 1103 62-63 0.5023811619807148 1103 64-65 0.5340227742125165 1103 66-67 0.6006844670800646 1103 68-69 0.6154128243820551 1103 70-71 0.6195172165860328 1103 72-73 0.6705835969848728 1103 74-75 0.8514915267209915 1103 76-77 0.643925502689541 1103 78-79 0.7806989017962991 1103 80-81 0.7821644193562634 1103 82-83 0.6586553099307899 1103 84-85 0.657787440407624 1103 86-87 0.5254747956404096 1103 88-89 0.6228949135796782 1103 90-91 0.7727366179950366 1103 92-93 1.0193402496068202 1103 94-95 0.976664024267631 1103 96-97 1.0260455478131298 1103 98-99 1.4227920984249671 1103 100-101 1.9543017388261 1104 1 0.19185464939098296 1104 2 0.16971252815904592 1104 3 0.11621637462414469 1104 4 0.0330692228066809 1104 5 0.03023999639486874 1104 6 0.046132095093355474 1104 7 0.1424372293822458 1104 8 0.12291387215594796 1104 9 0.2756058345614605 1104 10-11 0.16102344994315132 1104 12-13 0.18510519551715987 1104 14-15 0.2353721973692373 1104 16-17 0.19238402962032097 1104 18-19 0.15263912383709766 1104 20-21 0.1708541261394103 1104 22-23 0.06222560261429777 1104 24-25 0.213637843824948 1104 26-27 0.1837589560166819 1104 28-29 0.1655863075164774 1104 30-31 0.2529313339756243 1104 32-33 0.20950521964832092 1104 34-35 0.1761524362039566 1104 36-37 0.20167210682739523 1104 38-39 0.06634836471432237 1104 40-41 -0.0060865491312327435 1104 42-43 0.3760923105253937 1104 44-45 0.29414426716490993 1104 46-47 0.4974641441724401 1104 48-49 0.46139972643726423 1104 50-51 0.6707463575143393 1104 52-53 0.7395024737543778 1104 54-55 0.4685839620864485 1104 56-57 0.6315419134740594 1104 58-59 0.508885366507748 1104 60-61 0.5250492088056262 1104 62-63 0.4537324569891368 1104 64-65 0.4437661907666097 1104 66-67 0.5748843636795229 1104 68-69 0.49936280853842163 1104 70-71 0.5943583633314162 1104 72-73 0.6901905081437896 1104 74-75 0.773980728035184 1104 76-77 0.3565908184781357 1104 78-79 0.3889029844500236 1104 80-81 0.5386236178352739 1104 82-83 0.5659788202124751 1104 84-85 0.6834824755137738 1104 86-87 0.7137000587280831 1104 88-89 0.7103792784168235 1104 90-91 0.6963002677006784 1104 92-93 0.9074975518532788 1104 94-95 0.9835501669270243 1104 96-97 1.4072533328734202 1104 98-99 1.6251861545614545 1104 100-101 2.157589292402591 1105 1 0.1395478539212931 1105 2 0.04911792456144326 1105 3 -0.01608708973294881 1105 4 -0.023401463402514366 1105 5 -0.13544121613011129 1105 6 0.025454213680959015 1105 7 -0.031788286940205523 1105 8 -0.024029832041250643 1105 9 0.007709432163061081 1105 10-11 0.09534223741816561 1105 12-13 -0.053069354782643074 1105 14-15 0.07952609477096928 1105 16-17 0.08179109157901365 1105 18-19 0.1390398566818689 1105 20-21 0.04406781701216289 1105 22-23 0.09487883379350848 1105 24-25 0.17831555868171023 1105 26-27 0.17952844302534032 1105 28-29 -0.01685040134427851 1105 30-31 -0.0526774268504937 1105 32-33 0.057556518782227784 1105 34-35 0.06014644020130078 1105 36-37 0.20914212681407207 1105 38-39 0.2021411695111297 1105 40-41 0.08631435026916989 1105 42-43 0.09690343644144406 1105 44-45 0.2831141205959611 1105 46-47 0.3925091141924355 1105 48-49 0.2191029243053535 1105 50-51 0.09819888915991015 1105 52-53 0.13550097475371103 1105 54-55 0.24875967827566114 1105 56-57 0.3195374164720661 1105 58-59 0.1611005563811645 1105 60-61 0.25259334604746897 1105 62-63 0.232721464317585 1105 64-65 0.41384863580324804 1105 66-67 0.37999762150763416 1105 68-69 0.29791793845181047 1105 70-71 0.30978141463054953 1105 72-73 0.28865403246091503 1105 74-75 0.4315007147107295 1105 76-77 0.41665494239552814 1105 78-79 0.5331068485406298 1105 80-81 0.6470138576753826 1105 82-83 0.4889093665149389 1105 84-85 0.4843069258801975 1105 86-87 0.6267247089612624 1105 88-89 0.7018682857452703 1105 90-91 0.6920114602389873 1105 92-93 1.0197602100811274 1105 94-95 1.1218121589323538 1105 96-97 1.0826947052918108 1105 98-99 1.476310405061124 1105 100-101 1.9201059479655491 1106 1 -0.02858288683238186 1106 2 0.12620249481255286 1106 3 0.025380360411986658 1106 4 0.05999924556965652 1106 5 -0.05825798349643918 1106 6 -0.11004372575954591 1106 7 -0.09178156392829351 1106 8 -0.012862210765099746 1106 9 -0.013730427439497817 1106 10-11 0.07864690336305813 1106 12-13 -0.12247441188458197 1106 14-15 -0.034177498984483634 1106 16-17 0.06309413352362014 1106 18-19 0.2264892158129399 1106 20-21 0.14764345526173628 1106 22-23 0.13560762574685015 1106 24-25 0.13367366820568805 1106 26-27 -0.0366836199788807 1106 28-29 -0.044363672744417215 1106 30-31 -0.1314730909443682 1106 32-33 -0.1092929206757347 1106 34-35 -0.34930681303797684 1106 36-37 -0.13496388722567332 1106 38-39 -0.10156172574958333 1106 40-41 -0.14355830540784353 1106 42-43 -0.09020715273886282 1106 44-45 -0.006368949637689525 1106 46-47 0.04451335754799857 1106 48-49 -0.022141007521561562 1106 50-51 -0.0657982906675656 1106 52-53 0.12602087312271948 1106 54-55 0.018327353685151593 1106 56-57 -0.18795066546136496 1106 58-59 -0.13657694859561076 1106 60-61 -0.07079465905154336 1106 62-63 -0.0113181927214967 1106 64-65 -0.2172009835171167 1106 66-67 -0.04467583219962279 1106 68-69 -0.21951932798900486 1106 70-71 -0.009951061882567558 1106 72-73 0.19293340416489357 1106 74-75 0.2586734699232487 1106 76-77 0.16050273165965834 1106 78-79 0.26783862655900137 1106 80-81 0.39222327513076394 1106 82-83 0.3466620033326002 1106 84-85 0.34743382491278396 1106 86-87 0.35447328577821935 1106 88-89 0.5447811834507164 1106 90-91 0.5929836776105475 1106 92-93 0.7603748417997771 1106 94-95 0.7459484265256897 1106 96-97 1.0754238115677843 1106 98-99 1.094216754378344 1106 100-101 1.6547840292004885 1107 1 0.0856004855002368 1107 2 0.06424950350881176 1107 3 0.011544489214415421 1107 4 0.02923011554485555 1107 5 -0.2538622687616936 1107 6 -0.0811247336874601 1107 7 -0.02060407641388906 1107 8 0.022022799537694482 1107 9 -0.06202740994220335 1107 10-11 0.07790802689185483 1107 12-13 -0.003069354782631706 1107 14-15 0.14696030529728432 1107 16-17 0.048567407368487636 1107 18-19 0.1910135408923921 1107 20-21 0.09801518543321919 1107 22-23 0.19290514958298388 1107 24-25 0.18127608499749215 1107 26-27 0.023936337762187065 1107 28-29 0.022623282866241823 1107 30-31 -0.007282690008388215 1107 32-33 0.1026223082559099 1107 34-35 0.10159380862234713 1107 36-37 0.0482868636561804 1107 38-39 0.024838537932183158 1107 40-41 0.08894592921654265 1107 42-43 0.027166594336186733 1107 44-45 -0.05339903729878159 1107 46-47 0.17671964050822453 1107 48-49 0.2135108190421917 1107 50-51 0.19984362600201422 1107 52-53 0.19207992212213298 1107 54-55 0.20237809932828554 1107 56-57 0.4040768901562757 1107 58-59 0.546297924802218 1107 60-61 0.4946986092053649 1107 62-63 0.41989251694916163 1107 64-65 0.2783223200137783 1107 66-67 0.423747621507637 1107 68-69 0.36074688582023384 1107 70-71 0.32787351989370705 1107 72-73 0.40608824298722723 1107 74-75 0.37031650418441586 1107 76-77 0.17652336344815822 1107 78-79 0.375541059066947 1107 80-81 0.3341849103069592 1107 82-83 0.16423831388336296 1107 84-85 0.16753061009072567 1107 86-87 0.42475102475073356 1107 88-89 0.4268682857452717 1107 90-91 0.49299830234424746 1107 92-93 0.6743654732390247 1107 94-95 0.6540490010376168 1107 96-97 0.891576284239175 1107 98-99 1.1726919840084946 1107 100-101 1.9217506848076553 1108 1 0.06877268988387897 1108 2 0.04095619065107314 1108 3 0.007780354075507034 1108 4 -0.0422640209901175 1108 5 -0.1736078362613469 1108 6 -0.056995528047366406 1108 7 -0.04535278505552043 1108 8 -0.07723554193933779 1108 9 0.1310149605729407 1108 10-11 0.005186227366515084 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54-55 -0.9441853200898223 2316 56-57 -1.0143441624753038 2316 58-59 -0.8745360895815679 2316 60-61 -0.8571300112588389 2316 62-63 -1.021287198644167 2316 64-65 -1.0421112367041019 2316 66-67 -0.9578864909474092 2316 68-69 -0.9198309173834289 2316 70-71 -0.8864469279650677 2316 72-73 -0.8148295406021795 2316 74-75 -0.7778610048054517 2316 76-77 -0.8553804449859719 2316 78-79 -0.8100690259281507 2316 80-81 -0.6217034519029134 2316 82-83 -0.7989604438806133 2316 84-85 -0.8939253395660245 2316 86-87 -0.7163612668478265 2316 88-89 -0.772326712620206 2316 90-91 -0.6653397983422522 2316 92-93 -0.7798904110368845 2316 94-95 -0.9153343758829457 2316 96-97 -1.172535762181223 2316 98-99 -1.4650184605812413 2316 100-101 -2.0430396878631534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 8.0 5 19.0 6 50.0 7 149.0 8 344.0 9 639.0 10 1223.0 11 2093.0 12 3417.0 13 5064.0 14 6741.0 15 10124.0 16 14334.0 17 21222.0 18 19396.0 19 10104.0 20 7602.0 21 7368.0 22 7554.0 23 7710.0 24 9021.0 25 10334.0 26 12406.0 27 15220.0 28 19105.0 29 23946.0 30 30215.0 31 38227.0 32 49072.0 33 65426.0 34 90913.0 35 133313.0 36 204948.0 37 321011.0 38 262139.0 39 24303.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.47438339697158 20.19184909786159 15.90261256160452 20.43115494356231 2 15.424627325469556 21.424708175002998 40.67135779821629 22.479306701311156 3 19.481378483584418 26.188121399671722 30.76873216171289 23.561767955030962 4 12.254994208098626 17.71548173146487 38.092589572347194 31.936934488089314 5 12.476438637288854 39.04724262348106 33.994933609935366 14.481385129294722 6 29.874787857244918 37.85226152017926 17.316982223569713 14.955968399006109 7 26.99961317707081 35.01047209821817 20.43261440026764 17.557300324443375 8 26.55159555746063 35.113067296735004 19.237157304506315 19.09817984129805 9 26.846626451021592 16.878722299470645 20.70171770289908 35.572933546608674 10-11 24.682195342094353 28.072018762654512 26.340271751819984 20.90551414343115 12-13 26.130563076398715 25.65590484252464 27.737465903542617 20.476066177534026 14-15 22.994148867584588 26.186169860569503 27.609608542165443 23.21007272968047 16-17 20.417699065700653 29.915700480566503 28.16126682766864 21.5053336260642 18-19 20.333573790829856 27.323917157165113 32.27120817694884 20.071300875056195 20-21 21.615297010518802 25.890460033845287 32.76612042119819 19.72812253443772 22-23 21.083845264928957 26.08017915166418 32.640396107507726 20.195579475899137 24-25 20.714615536074913 26.326853144172446 32.90653647044884 20.051994849303803 26-27 20.50201272770169 26.4318838650366 32.96590438227852 20.100199024983194 28-29 20.30031403088313 26.70280118614528 32.97519431336107 20.021690469610515 30-31 20.507813861292288 26.420134963526948 32.8796215110841 20.19242966409666 32-33 20.472481556213037 26.545740940153962 32.664791795172036 20.316985708460965 34-35 20.83658299442766 26.455064069725704 32.677058612386 20.031294323460635 36-37 20.850753451701273 26.3304021882352 32.581049071850515 20.237795288213007 38-39 20.555875003920505 26.66293783302492 32.65649775398759 20.12468940906699 40-41 20.749774353291308 26.57473523538698 32.562649632518216 20.1128407788035 42-43 20.560978924953037 26.625343728544195 32.23973345136567 20.573943895137088 44-45 20.871153115667028 26.731729586378254 31.889891375939616 20.507225922015103 46-47 21.065157011775447 27.200935344812567 31.079479914829257 20.654427728582732 48-49 21.05156593588497 27.38922401926448 30.758242639038446 20.800967405812102 50-51 21.198349555502123 27.70216725387084 30.401076134419224 20.69840705620781 52-53 21.25972589926779 28.08056653199881 29.91114569673281 20.748561872000597 54-55 21.158656644119418 27.875923931793707 29.95110697570682 21.01431244838005 56-57 21.310423658229745 28.121399671723246 29.761668287141102 20.80650838290591 58-59 21.504636647813406 27.661394026199414 30.08091917491715 20.753050151070035 60-61 21.195910131624345 27.813195889222275 30.44055995232669 20.550334026826693 62-63 21.149003495345926 27.738026784874176 30.50879412307939 20.604175596700504 64-65 21.130324478224658 27.74531020759497 30.42170669064272 20.702658623537655 66-67 21.047906800068304 27.963046213142917 29.94155837367095 21.047488613117828 68-69 21.088296689701796 27.933982220084825 29.957588873439207 21.020132216774176 70-71 21.33185573944165 27.858081288573388 29.842482915320627 20.967580056664332 72-73 21.242719624021923 27.8082918834415 29.830156795766833 21.11883169676975 74-75 21.017936735284177 28.387993852651828 29.367108899366794 21.2269605126972 76-77 21.116489459946404 27.937397413513708 29.814673483113957 21.131439643425924 78-79 21.114259129543864 28.036681965339273 29.598540527542838 21.25051837757403 80-81 21.16872797984339 28.375134603924685 29.327102347771238 21.129035068460688 82-83 21.09943555157348 28.37969463259973 29.39857335372694 21.12229646209985 84-85 21.11575416245429 28.253008080442694 29.316007793635247 21.31522996346777 86-87 21.019086749397985 28.192770244604514 29.35553906040362 21.43260394559388 88-89 21.106313577484816 28.074388488707207 29.34926625614648 21.4700316776615 90-91 21.179359963420108 28.022720239107585 29.343653026344775 21.454266771127536 92-93 21.154494228330407 27.994139637358696 29.43490505313393 21.416461081176966 94-95 20.89941558373671 27.96482350768244 29.494760465999658 21.64100044258119 96-97 20.86087268646782 27.997372391994507 29.44447348520489 21.69728143633278 98-99 20.88153962763872 28.00801526385029 29.14520695933996 21.96523814917103 100-101 20.66238926748987 27.93956759209658 29.627013068849767 21.771030071563775 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 905.0 1 631.5 2 487.0 3 681.5 4 574.5 5 489.0 6 1020.0 7 2465.0 8 4010.0 9 4760.5 10 4284.5 11 3554.0 12 3587.0 13 3700.0 14 3854.5 15 4091.0 16 4165.5 17 4211.0 18 4382.0 19 4575.5 20 4854.5 21 5333.5 22 6026.0 23 7033.5 24 8143.5 25 9483.5 26 11769.5 27 14356.0 28 16226.5 29 18505.0 30 21199.0 31 23177.0 32 25911.0 33 29159.0 34 32618.5 35 35784.5 36 39851.0 37 43388.5 38 44676.0 39 45833.5 40 46677.0 41 47688.0 42 48014.5 43 49081.5 44 51135.5 45 52163.0 46 52447.0 47 54070.0 48 58923.0 49 57355.5 50 50115.5 51 45165.0 52 41345.0 53 39314.0 54 37656.5 55 34418.0 56 30101.0 57 26367.0 58 23290.0 59 20529.0 60 17243.0 61 13821.0 62 10741.0 63 7714.5 64 5521.0 65 4107.0 66 2969.0 67 2190.0 68 1514.5 69 991.0 70 707.5 71 524.5 72 397.0 73 343.5 74 278.5 75 210.5 76 137.5 77 77.0 78 52.5 79 28.0 80 15.0 81 6.0 82 3.5 83 2.0 84 1.5 85 1.0 86 0.5 87 1.5 88 2.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0430035580739703 2 6.96978250793684E-5 3 0.0 4 2.0909347523810517E-4 5 0.01484563674190547 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0033803445163493673 14-15 0.0 16-17 0.0 18-19 0.0 20-21 6.96978250793684E-5 22-23 2.0909347523810517E-4 24-25 5.22733688095263E-4 26-27 0.001428805414127052 28-29 0.0023348771401588415 30-31 4.878847755555788E-4 32-33 0.0 34-35 0.0 36-37 1.0454673761905259E-4 38-39 0.0 40-41 0.0 42-43 0.009060717260317892 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.48489125396842E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.48489125396842E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.0454673761905259E-4 84-85 3.136402128571578E-4 86-87 0.0 88-89 0.0 90-91 0.006760689032698735 92-93 0.0028924597407937883 94-95 0.0 96-97 0.0 98-99 1.74244562698421E-4 100-101 0.011883479176032313 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1434765.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.39277582138643 #Duplication Level Percentage of deduplicated Percentage of total 1 75.00298584886664 40.04617609363155 2 14.032314820125666 14.984484788921762 3 4.707227722966156 7.539958636576419 4 2.1430806701221847 4.577001031479217 5 1.123164951354666 2.9984447229059037 6 0.6655929103670991 2.1322711830920804 7 0.42939862132726553 1.6048749028577363 8 0.3036765982277006 1.2971309225098282 9 0.2164644323824672 1.040187322035066 >10 1.2398768407060496 12.195012672342138 >50 0.08770254355539758 3.2657432781151616 >100 0.044711634439932674 4.2257413286131795 >500 0.002753466094309833 1.0529788531872464 >1k 6.55587165311865E-4 0.6869110104031154 >5k 1.31117433062373E-4 0.5802899883032434 >10k+ 2.62234866124746E-4 1.7727932650263036 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13618 0.949144981930839 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11705 0.8158130425540071 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8289 0.5777252720828846 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2789 0.19438723414635845 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2614 0.182190114757469 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2164 0.15082609347175321 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 6.96978250793684E-5 0.0 24-25 0.0 0.0 0.0 1.74244562698421E-4 0.0 26-27 0.0 0.0 0.0 5.22733688095263E-4 0.0 28-29 0.0 0.0 0.0 0.0012197119388889471 6.96978250793684E-5 30-31 0.0 0.0 0.0 0.0034151934288890514 6.96978250793684E-5 32-33 0.0 0.0 0.0 0.006098559694444734 6.96978250793684E-5 34-35 0.0 0.0 0.0 0.007806156408889261 6.96978250793684E-5 36-37 0.0 0.0 0.0 0.01129104766285768 6.96978250793684E-5 38-39 0.0 0.0 0.0 0.017424456269842098 6.96978250793684E-5 40-41 0.0 0.0 0.0 0.022860886626032834 6.96978250793684E-5 42-43 0.0 0.0 0.0 0.028541259370001362 6.96978250793684E-5 44-45 0.0 0.0 0.0 0.030910985422699884 6.96978250793684E-5 46-47 0.0 0.0 0.0 0.03429132993904925 6.96978250793684E-5 48-49 0.0 0.0 0.0 0.039274724432224094 6.96978250793684E-5 50-51 0.0 0.0 0.0 0.04502479500127199 6.96978250793684E-5 52-53 0.0 0.0 0.0 0.057431007865399564 6.96978250793684E-5 54-55 0.0 0.0 0.0 0.06520231536174914 6.96978250793684E-5 56-57 0.0 0.0 0.0 0.08614651179809935 6.96978250793684E-5 58-59 0.0 0.0 0.0 0.12266817213968839 6.96978250793684E-5 60-61 0.0 0.0 0.0 0.15598373252762648 6.96978250793684E-5 62-63 0.0 0.0 0.0 0.17501123877429403 6.96978250793684E-5 64-65 0.0 0.0 0.0 0.20832679916223212 6.96978250793684E-5 66-67 0.0 0.0 0.0 0.26986997870731444 6.96978250793684E-5 68-69 0.0 0.0 0.0 0.3609650360860489 6.96978250793684E-5 70-71 0.0 0.0 0.0 0.44829641091049754 6.96978250793684E-5 72-73 0.0 0.0 0.0 0.5378581161374859 6.96978250793684E-5 74-75 0.0 0.0 0.0 0.652720131868285 6.96978250793684E-5 76-77 0.0 0.0 0.0 0.736252975225908 6.96978250793684E-5 78-79 0.0 0.0 0.0 0.8426118562970242 6.96978250793684E-5 80-81 0.0 0.0 0.0 0.9828090314441738 6.96978250793684E-5 82-83 0.0 0.0 0.0 1.1128651730422752 6.96978250793684E-5 84-85 0.0 0.0 0.0 1.2662700860419651 6.96978250793684E-5 86-87 0.0 0.0 0.0 1.4685680233348317 6.96978250793684E-5 88-89 0.0 0.0 0.0 1.7287848532686536 6.96978250793684E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9855 0.0 31.823254 1 CGTACAG 95 3.4907684E-5 30.000849 5 GGACCGA 305 0.0 29.588938 6 TAGCGCA 65 0.0058437423 29.231594 5 GTATCAA 13790 0.0 28.290209 1 TACATGG 9040 0.0 23.120123 2 TACCTGG 1050 0.0 23.07208 2 GTACATG 9180 0.0 22.982515 1 GAGTACT 10500 0.0 22.8693 12-13 CCGTAAG 125 2.2505327E-4 22.808594 1 AGGACCG 375 0.0 22.800644 5 TATCAAC 17155 0.0 22.76077 2 ATCAACG 17145 0.0 22.746342 3 TCAACGC 17290 0.0 22.528109 4 CAACGCA 17525 0.0 22.22602 5 AGTACTT 10910 0.0 21.901014 12-13 ACATGGG 9125 0.0 21.863634 3 AACGCAG 17855 0.0 21.813713 6 GTACTTT 11170 0.0 21.28269 14-15 CCGTACA 135 3.7958554E-4 21.111706 4 >>END_MODULE