FastQCFastQC Report
Thu 26 May 2016
SRR937627_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937627_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292680
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111630.8635547854070613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73390.5677352477024477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52730.40791224432960976No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46860.3625027075533001No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22730.1758362471764087No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21950.1698022712504255No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19310.14937958350094377No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18960.14667203020082312No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18720.1448154222235975No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16590.1283380264257202No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16340.12640405978277688No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15000.11603799857660055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14630.11317572794504441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGC1152.5011104E-937.1762968
TCGCGCA1152.5011104E-937.1762969
CGTTCGC1252.1530468E-730.4019476
TCGTTCG1354.1877502E-728.1772635
GTTCGCG1457.8817357E-726.2095937
GTACATG116350.024.8103181
TACATGG116650.024.6136952
CATGGGG27000.024.3010034
GTATCAA177250.024.2544921
GAGTACT81250.023.9410712-13
TACCTGG10650.023.6565232
ACATGGG117900.023.5069473
TAGCCCT8650.022.5359884
AGTACTT84950.022.36709812-13
GTACTTT87500.021.95742614-15
ATCAACG203550.020.972333
GTACCTG12750.020.8875661
TCAACGC204450.020.836744
TATCAAC204350.020.8196322
CAACGCA205800.020.7016625
Produced by FastQC (version 0.11.5)