##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937627_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1292680 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.932335148683354 33.0 31.0 34.0 30.0 34.0 2 32.03447179503048 33.0 31.0 34.0 30.0 34.0 3 32.059057152582234 34.0 31.0 34.0 30.0 34.0 4 35.649222545409536 37.0 35.0 37.0 35.0 37.0 5 35.66337763406257 37.0 35.0 37.0 35.0 37.0 6 35.75657780734598 37.0 35.0 37.0 35.0 37.0 7 35.71117213850295 37.0 35.0 37.0 35.0 37.0 8 35.71152876195191 37.0 35.0 37.0 35.0 37.0 9 37.35255360955534 39.0 37.0 39.0 35.0 39.0 10-11 37.241897066559396 39.0 37.0 39.0 34.0 39.0 12-13 37.140211808026734 39.0 37.0 39.0 34.0 39.0 14-15 38.263368351022685 40.0 38.0 41.0 33.5 41.0 16-17 38.22298674072469 40.0 38.0 41.0 33.5 41.0 18-19 38.23884681437015 40.0 38.0 41.0 33.5 41.0 20-21 38.17955217068416 40.0 38.0 41.0 34.0 41.0 22-23 38.09877734628833 40.0 38.0 41.0 33.5 41.0 24-25 38.12450567812606 40.0 38.0 41.0 34.0 41.0 26-27 38.02798101618343 40.0 38.0 41.0 33.5 41.0 28-29 37.913471624841414 40.0 38.0 41.0 33.0 41.0 30-31 37.64699887056348 40.0 37.0 41.0 33.0 41.0 32-33 37.443832580375656 40.0 36.5 41.0 33.0 41.0 34-35 37.412000263019465 40.0 36.0 41.0 33.0 41.0 36-37 37.25856785902157 40.0 36.0 41.0 32.5 41.0 38-39 37.08010451155738 40.0 36.0 41.0 32.0 41.0 40-41 36.8310842590587 39.5 36.0 41.0 31.0 41.0 42-43 36.5045622273107 39.0 35.0 41.0 30.5 41.0 44-45 36.14400625058019 39.0 35.0 41.0 30.0 41.0 46-47 35.626820636197664 39.0 35.0 41.0 27.5 41.0 48-49 35.18598028901198 39.0 35.0 40.5 25.0 41.0 50-51 34.24794767459851 38.0 34.0 39.5 23.5 40.5 52-53 34.31128546894823 38.0 34.5 40.0 23.0 40.5 54-55 34.61092691153263 39.0 35.0 40.0 23.0 41.0 56-57 34.405082851130985 38.0 34.5 40.0 22.5 41.0 58-59 34.185530061577495 38.0 34.0 40.0 21.5 41.0 60-61 33.86333121886314 38.0 34.0 40.0 19.5 41.0 62-63 33.5225945322895 38.0 33.5 40.0 18.0 41.0 64-65 33.156724015224185 37.0 33.0 40.0 15.5 41.0 66-67 32.72715675960021 36.5 32.5 39.0 11.5 41.0 68-69 32.24291471980691 36.0 32.0 39.0 7.5 40.5 70-71 31.796306510505307 35.5 31.5 39.0 6.5 40.0 72-73 31.34406891109942 35.0 31.0 38.0 2.0 40.0 74-75 30.83504695671009 35.0 31.0 37.0 2.0 39.0 76-77 30.375409614134973 35.0 30.0 37.0 2.0 39.0 78-79 29.837859717795588 34.0 29.5 36.0 2.0 39.0 80-81 29.295752622458767 34.0 29.0 36.0 2.0 37.5 82-83 28.812496518860044 34.0 29.0 35.0 2.0 37.0 84-85 28.32126125568586 34.0 28.0 35.0 2.0 36.5 86-87 27.83427143608627 34.0 26.5 35.0 2.0 36.0 88-89 27.406782034223475 33.0 26.0 35.0 2.0 36.0 90-91 26.891442971191633 33.0 25.5 35.0 2.0 35.0 92-93 26.324712225763527 33.0 24.5 35.0 2.0 35.0 94-95 25.64117298944828 32.0 21.5 34.5 2.0 35.0 96-97 24.877312637311633 32.0 18.0 34.0 2.0 35.0 98-99 23.93275017792493 31.5 2.0 34.0 2.0 35.0 100-101 21.190395534857814 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28702346481430396 1101 2 0.3491559906044799 1101 3 0.2765419731597518 1101 4 0.04337635768807502 1101 5 -0.061782843903714024 1101 6 -0.02355051851093748 1101 7 0.11337216256023197 1101 8 0.13552716704077028 1101 9 0.11883883442882848 1101 10-11 0.173712229808757 1101 12-13 0.12045947139244362 1101 14-15 0.3236852008808242 1101 16-17 0.26220278237189376 1101 18-19 0.23600144568875692 1101 20-21 0.2516180425943446 1101 22-23 0.13596679641351272 1101 24-25 0.06636573192924544 1101 26-27 0.04603741434666375 1101 28-29 0.06636008224580792 1101 30-31 0.17422264661198028 1101 32-33 0.12960156769684517 1101 34-35 0.07501585307672087 1101 36-37 0.15347966862470486 1101 38-39 0.04045723096461984 1101 40-41 0.19448206700977977 1101 42-43 0.28165231468309315 1101 44-45 0.34640084979195507 1101 46-47 0.3837283383361765 1101 48-49 0.40903040204053553 1101 50-51 0.3262834785965225 1101 52-53 0.2438195730659416 1101 54-55 0.5506249344893419 1101 56-57 0.5170869051773508 1101 58-59 0.3106589608018524 1101 60-61 0.3342805996984026 1101 62-63 0.4491647476021541 1101 64-65 0.4743796942381948 1101 66-67 0.7131956109608169 1101 68-69 0.6205053207089293 1101 70-71 0.5988276005880699 1101 72-73 0.651322203997271 1101 74-75 0.6677037377208244 1101 76-77 0.6369275294552246 1101 78-79 0.6540602879082691 1101 80-81 0.6125961831632303 1101 82-83 0.6423110488881285 1101 84-85 0.6386212061212078 1101 86-87 0.7373558846322688 1101 88-89 0.71755296599218 1101 90-91 0.6645134188626152 1101 92-93 0.5289014623024038 1101 94-95 0.9085407923310562 1101 96-97 1.0744281713728014 1101 98-99 1.2901616012529473 1101 100-101 1.579192340061443 1102 1 0.167047135769387 1102 2 0.24096737782495836 1102 3 0.3375960296065941 1102 4 0.1141725498328583 1102 5 -0.004391262003608176 1102 6 0.11059693103684509 1102 7 0.2536260661709804 1102 8 0.15780023107816277 1102 9 0.19746438135370425 1102 10-11 0.20747479335455665 1102 12-13 0.296616459216807 1102 14-15 0.23342337588746176 1102 16-17 0.4120479659382923 1102 18-19 0.33135042918898705 1102 20-21 0.4439940613074498 1102 22-23 0.4906915516660888 1102 24-25 0.3782742492794071 1102 26-27 0.2818028062920135 1102 28-29 0.39148624191292214 1102 30-31 0.36534930020059875 1102 32-33 0.43661222428627156 1102 34-35 0.3535194340076231 1102 36-37 0.4992060827079854 1102 38-39 0.4056813128996737 1102 40-41 0.37411922108999107 1102 42-43 0.5040311524764718 1102 44-45 0.41481771488148667 1102 46-47 0.26191727729028 1102 48-49 0.4165394067887007 1102 50-51 0.4062212957430589 1102 52-53 0.45477589363031257 1102 54-55 0.3097974295850676 1102 56-57 0.3255234106361158 1102 58-59 0.2784142739527411 1102 60-61 0.2871722538228809 1102 62-63 0.49147716227255245 1102 64-65 0.4795909155755993 1102 66-67 0.35788548883173377 1102 68-69 0.5301875800736298 1102 70-71 0.4936694991104993 1102 72-73 0.4691513723918881 1102 74-75 0.3561423633143441 1102 76-77 0.40762039415334783 1102 78-79 0.7024931355521247 1102 80-81 0.5059167102613493 1102 82-83 0.6129343685065933 1102 84-85 0.6333098025919988 1102 86-87 0.9956983819180287 1102 88-89 0.996917997281642 1102 90-91 0.9314191637288616 1102 92-93 0.9858079760087257 1102 94-95 1.2183076241041881 1102 96-97 1.3917890462662115 1102 98-99 1.2795364643761111 1102 100-101 1.5268628711202474 1103 1 0.17618413006833578 1103 2 0.25289321844013557 1103 3 0.2056588740915899 1103 4 0.06626235015252746 1103 5 0.06379430235360672 1103 6 0.14811316164386312 1103 7 0.11809664264598752 1103 8 0.19781126948607408 1103 9 0.20837570933372973 1103 10-11 0.14523646370600574 1103 12-13 0.25211269307792605 1103 14-15 0.3507210821743527 1103 16-17 0.46339517278507714 1103 18-19 0.24857034553954804 1103 20-21 0.2884165813610906 1103 22-23 0.17684425176913265 1103 24-25 0.333277848991429 1103 26-27 0.31974015535136147 1103 28-29 0.262701094207749 1103 30-31 0.24967465461538296 1103 32-33 0.2913189142208026 1103 34-35 0.44524084075593606 1103 36-37 0.4541413570145494 1103 38-39 0.5759907605219965 1103 40-41 0.5667774568801676 1103 42-43 0.5502470416159753 1103 44-45 0.42523880143090054 1103 46-47 0.3683182777656313 1103 48-49 0.47799611506740547 1103 50-51 0.46069950764858447 1103 52-53 0.5302672250771181 1103 54-55 0.46539351956404573 1103 56-57 0.47291832509909426 1103 58-59 0.6746670641283501 1103 60-61 0.7943325968004658 1103 62-63 0.6764925235251908 1103 64-65 0.7581230200087248 1103 66-67 0.7987483881897433 1103 68-69 0.6516837097185615 1103 70-71 0.7680603395714733 1103 72-73 0.7219537774461564 1103 74-75 0.5748575691997893 1103 76-77 0.7281776742385944 1103 78-79 0.6694967501521738 1103 80-81 0.5905439725766719 1103 82-83 0.8625975269162574 1103 84-85 0.5991609244579372 1103 86-87 0.7358430403601233 1103 88-89 0.8933778905344951 1103 90-91 1.0150938582318823 1103 92-93 0.9153248701963328 1103 94-95 1.1144471589384466 1103 96-97 1.6957719654465606 1103 98-99 1.7925871485340856 1103 100-101 1.8698954875951266 1104 1 0.21944537672923303 1104 2 0.32731585236123095 1104 3 0.292527473819014 1104 4 0.23085751349523775 1104 5 0.17111450418396146 1104 6 0.1659725602814177 1104 7 0.25221955047384625 1104 8 0.21791060305151433 1104 9 0.23537919812737584 1104 10-11 0.21862103516568254 1104 12-13 0.42464468224489593 1104 14-15 0.46672218724999937 1104 16-17 0.4142472929178922 1104 18-19 0.4366262853724976 1104 20-21 0.43641994793912886 1104 22-23 0.48318539355054213 1104 24-25 0.5137949340155004 1104 26-27 0.5108910259065382 1104 28-29 0.4878309868120638 1104 30-31 0.4285292478171172 1104 32-33 0.5120351798867375 1104 34-35 0.5054605310266567 1104 36-37 0.46833069262375915 1104 38-39 0.6977308643949982 1104 40-41 0.7234244971568842 1104 42-43 0.6386715796358189 1104 44-45 0.7589036061067276 1104 46-47 0.8295954412130939 1104 48-49 0.8758004961377424 1104 50-51 0.8174638338348714 1104 52-53 0.8597923609264555 1104 54-55 1.062403961296475 1104 56-57 1.0158966272058194 1104 58-59 0.9160536875779357 1104 60-61 1.095036414960063 1104 62-63 0.858480304017732 1104 64-65 0.9642820194428339 1104 66-67 1.1879488926629378 1104 68-69 1.1417300694905208 1104 70-71 0.9877531778391351 1104 72-73 0.8726227795607961 1104 74-75 1.130427674233328 1104 76-77 1.089996489075773 1104 78-79 1.0036560659090519 1104 80-81 1.1010719748443343 1104 82-83 1.0858718475668958 1104 84-85 1.288368412680562 1104 86-87 1.2445352408471386 1104 88-89 1.1963529677922224 1104 90-91 1.3918075727752317 1104 92-93 1.4158925565931213 1104 94-95 1.3088398243583086 1104 96-97 1.5635772913771184 1104 98-99 1.3040140517855328 1104 100-101 1.1481930240775142 1105 1 0.13309738202210752 1105 2 0.26457122570373315 1105 3 0.20871862931117136 1105 4 0.16974604917248115 1105 5 0.10802814526176974 1105 6 0.1256590805330262 1105 7 0.08771818155796751 1105 8 0.09701983419365945 1105 9 0.1520958663172678 1105 10-11 0.2159244298386298 1105 12-13 0.24516620144348877 1105 14-15 0.3427543378982918 1105 16-17 0.33999950126331413 1105 18-19 0.4014661083074955 1105 20-21 0.2858471490326693 1105 22-23 0.3208630645259518 1105 24-25 0.3201266169674284 1105 26-27 0.3839513972820896 1105 28-29 0.3646782285161194 1105 30-31 0.48820821816985216 1105 32-33 0.5200460199520691 1105 34-35 0.5904152713697357 1105 36-37 0.5273142775541686 1105 38-39 0.40497310488709815 1105 40-41 0.4277481056906609 1105 42-43 0.605670574307176 1105 44-45 0.5418559344557963 1105 46-47 0.5147398230949278 1105 48-49 0.6598003729551891 1105 50-51 0.5130151552658546 1105 52-53 0.5184410681056093 1105 54-55 0.7242539249377486 1105 56-57 0.6349341939131463 1105 58-59 0.6539594449718678 1105 60-61 0.8454402352088977 1105 62-63 0.8037907240702609 1105 64-65 0.7466671119688328 1105 66-67 0.7890514759204379 1105 68-69 0.7968044955988631 1105 70-71 0.8467747109454429 1105 72-73 0.9900962257717403 1105 74-75 0.9685921150043733 1105 76-77 0.9996684062335177 1105 78-79 1.0916948406002014 1105 80-81 1.1056356900303612 1105 82-83 1.0719899677215494 1105 84-85 1.0291019052240422 1105 86-87 0.8412514322013109 1105 88-89 0.6951370367108787 1105 90-91 0.8652210012637838 1105 92-93 1.0278118600950812 1105 94-95 1.2391691588943274 1105 96-97 1.511079665621633 1105 98-99 1.3600268508509359 1105 100-101 1.6098857312725663 1106 1 -0.08005555202597847 1106 2 0.03030812494549906 1106 3 0.02291802508156593 1106 4 0.04374566206158903 1106 5 -0.01121673603825002 1106 6 0.012769970972229316 1106 7 -0.07238793731873727 1106 8 0.0861070940376365 1106 9 0.013289261868862923 1106 10-11 -0.2028948398682573 1106 12-13 -0.20970697766261281 1106 14-15 -0.2649645270252492 1106 16-17 -0.2658143152292922 1106 18-19 -0.0809403328810987 1106 20-21 -0.18628402265100874 1106 22-23 -0.15053596264755242 1106 24-25 -0.24785868123418453 1106 26-27 -0.20969156866056693 1106 28-29 -0.07843224956582873 1106 30-31 -0.0836113713243023 1106 32-33 -0.10046433526425602 1106 34-35 0.14415320571236379 1106 36-37 0.06821443660624738 1106 38-39 0.10250274832430506 1106 40-41 0.10711299136671215 1106 42-43 0.2810959755139564 1106 44-45 0.18582016036025095 1106 46-47 0.3433538398753484 1106 48-49 0.24339739051823983 1106 50-51 0.06045938184354327 1106 52-53 0.275379610044034 1106 54-55 0.05033068229495541 1106 56-57 0.11847882527418108 1106 58-59 0.20931945809997643 1106 60-61 0.2958931970279792 1106 62-63 0.33303758300199604 1106 64-65 0.3792147224006328 1106 66-67 0.21770946344873465 1106 68-69 0.12847752995917006 1106 70-71 0.1279192547857484 1106 72-73 0.29545097924101427 1106 74-75 0.2202152457216222 1106 76-77 0.17277745368826203 1106 78-79 0.396115478659933 1106 80-81 0.2882016040749136 1106 82-83 0.14571460564663496 1106 84-85 0.25637807518255684 1106 86-87 0.448129803642626 1106 88-89 0.6525506310354956 1106 90-91 0.6478256263973812 1106 92-93 0.5006877994701462 1106 94-95 0.6592412793363067 1106 96-97 0.5807301718112008 1106 98-99 0.712449239325128 1106 100-101 0.8537582287268926 1107 1 -0.02258844074847488 1107 2 0.030473548036816567 1107 3 -0.003096932648482209 1107 4 -0.051381321927195245 1107 5 -0.18135438546318738 1107 6 -0.050071214153625476 1107 7 0.004871176187627668 1107 8 -0.06738376851318861 1107 9 -0.07220835690927174 1107 10-11 -0.028111195858748772 1107 12-13 0.024730192035356424 1107 14-15 -0.01267581149202357 1107 16-17 0.11014230909486145 1107 18-19 0.14373469780923642 1107 20-21 0.13276923487791237 1107 22-23 -0.05350878972818407 1107 24-25 -0.04175456916439657 1107 26-27 0.06530690770569691 1107 28-29 0.0016651759284727063 1107 30-31 0.04715558236594575 1107 32-33 -0.006422396157368837 1107 34-35 0.18901007120597058 1107 36-37 0.13728293047253715 1107 38-39 0.04403112709094614 1107 40-41 0.03858974071589927 1107 42-43 0.06396510843575953 1107 44-45 0.11980219889512966 1107 46-47 0.11974752941973321 1107 48-49 0.17406008412855556 1107 50-51 0.30160598152836826 1107 52-53 0.2073624234554856 1107 54-55 0.1491383300656608 1107 56-57 0.2188307846671691 1107 58-59 0.3063771036940608 1107 60-61 0.36429564528972946 1107 62-63 0.3019554547904022 1107 64-65 0.2760494821453534 1107 66-67 0.2868519623821939 1107 68-69 0.3716712403991096 1107 70-71 0.48203676612593327 1107 72-73 0.4168530270047519 1107 74-75 0.43786414827000897 1107 76-77 0.4277858554400069 1107 78-79 0.46593925953528625 1107 80-81 0.7298279307245714 1107 82-83 0.921756771121963 1107 84-85 0.8836450245195593 1107 86-87 1.0143186297481286 1107 88-89 0.8344790289563875 1107 90-91 0.8096431473146914 1107 92-93 0.9507496154674335 1107 94-95 1.2068025947110783 1107 96-97 1.2790038947884668 1107 98-99 1.1082588112275822 1107 100-101 1.4484161511869935 1108 1 0.01807543648194354 1108 2 -0.04724706563915504 1108 3 -0.005487187243637948 1108 4 -0.022396865835027313 1108 5 0.05575091897863871 1108 6 0.09230715632563147 1108 7 0.17139009729041987 1108 8 0.1140273681713353 1108 9 0.13929828250773824 1108 10-11 0.19213096717928835 1108 12-13 0.18117179116886462 1108 14-15 0.10466989378620184 1108 16-17 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-0.31815897450467645 2316 56-57 -0.10484890004241976 2316 58-59 -0.10109196173701918 2316 60-61 -0.1204058709811946 2316 62-63 -0.12607305051581363 2316 64-65 -0.19256703684105503 2316 66-67 -0.1584523192850682 2316 68-69 -0.2814163451632936 2316 70-71 -0.16288355503580476 2316 72-73 -0.13054756404244827 2316 74-75 -0.3466103432483685 2316 76-77 -0.49945491311706647 2316 78-79 -0.3181731666449785 2316 80-81 -0.40621812007221436 2316 82-83 -0.38116348154092705 2316 84-85 -0.38319101370279895 2316 86-87 -0.17324956330119434 2316 88-89 -0.44498526112644754 2316 90-91 -0.5476725296251708 2316 92-93 -1.030961803779764 2316 94-95 -0.941196505018354 2316 96-97 -1.1900780494045442 2316 98-99 -1.0549268500064741 2316 100-101 -1.1515903317396656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4259.0 3 620.0 4 506.0 5 598.0 6 731.0 7 897.0 8 1115.0 9 1439.0 10 2029.0 11 2854.0 12 4655.0 13 6686.0 14 7773.0 15 10774.0 16 14871.0 17 21526.0 18 17108.0 19 9462.0 20 8268.0 21 8422.0 22 9187.0 23 9886.0 24 10975.0 25 12539.0 26 14389.0 27 17106.0 28 20567.0 29 24742.0 30 30589.0 31 38404.0 32 49363.0 33 64338.0 34 88183.0 35 125015.0 36 179654.0 37 253875.0 38 197785.0 39 21489.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.30368048172951 19.667646990354545 14.506902358208723 20.52177016970722 2 14.609279670844971 21.617166452437672 41.52510357849042 22.248450298226928 3 20.108739075089535 27.642825726588484 29.303078561615838 22.945356636706148 4 11.969796051983433 16.66119774758107 38.48140916590839 32.88759703452711 5 12.407835531419705 39.71326609775019 32.98681148176881 14.892086889061288 6 29.345718187722937 37.05008146162778 18.316154825148157 15.288045525501131 7 27.267217178191807 35.24849751277715 20.419022255567768 17.06526305346328 8 26.21268620564839 34.05775814561303 19.921281874601313 19.808273774137266 9 27.702712652872048 17.613448322098755 21.202855652516288 33.48098337251291 10-11 24.395633867447167 28.29467519099117 26.41452835102689 20.895162590534774 12-13 26.347858133535468 25.9063516403712 27.592857616190457 20.152932609902873 14-15 23.148556128967755 26.396931708661132 27.989555346548812 22.4649568158223 16-17 20.23396685631017 30.249236516967148 28.203063233447534 21.313733393275147 18-19 20.098107033393827 28.23619318668432 32.09641585353235 19.569283926389495 20-21 21.81219600658439 26.683724397903163 31.889316171752213 19.614763423760234 22-23 20.940183028640803 27.041344385162258 31.825686861144447 20.192785725052495 24-25 20.616679486707522 27.109368188344163 32.32917214828363 19.944780176664683 26-27 20.468633449516506 27.04848887605858 32.5468914381591 19.935986236265812 28-29 19.9471925553362 27.79719398926799 32.44142776200453 19.814185693391277 30-31 20.240554436351207 27.504431816684406 32.24827785068324 20.00673589628115 32-33 19.979101957796185 27.53177533364391 32.187325833499344 20.30179687506056 34-35 20.52627111713823 27.5127694035054 32.05832436435907 19.9026351149973 36-37 20.58600280912481 27.168382815905456 32.17982273453771 20.065791640432025 38-39 20.308385882965553 27.76143216021994 32.13495892372568 19.79522303308883 40-41 20.352745849792417 27.843945608015826 31.892114944621923 19.911193597569834 42-43 20.009183928512613 28.05112774379552 31.346026788395697 20.59366153929617 44-45 20.43927690635962 27.96229211135344 30.983914545733565 20.61451643655337 46-47 20.837788369536195 28.37956559666999 30.242755713910913 20.539890319882904 48-49 20.785438942620516 28.442302155039282 30.02266687903544 20.74959202330476 50-51 20.80390060298746 28.88817235388338 29.867663649339484 20.44026339378968 52-53 20.787158856738824 29.340297429011347 29.3090535828086 20.563490131441235 54-55 20.50181058684714 28.886154871534554 29.506850005134353 21.105184536483954 56-57 20.97651295077026 29.25707125926259 28.920197033647128 20.846218756320024 58-59 21.176180690514492 28.79960418933202 29.220172396157817 20.80404272399567 60-61 20.91172850530779 28.860606685167056 29.80303400695876 20.424630802566394 62-63 20.876153355895166 28.82193521641344 29.819744251866 20.48216717582539 64-65 20.70728046596718 29.130619737277453 29.613471020012195 20.548628776743175 66-67 20.769441168585434 29.098835722687213 29.10952853974861 21.022194568978744 68-69 20.611537662772424 29.109567810348626 29.32732096970674 20.951573557172214 70-71 20.886665687741836 29.05012392181051 29.135515095912034 20.927695294535624 72-73 20.77934556048878 28.858605112469682 29.264820970578892 21.09722835646265 74-75 20.455499088157204 29.72161964357737 28.81031926314888 21.012562005116546 76-77 20.53817052398938 29.234040014072654 28.980561759267726 21.24722770267024 78-79 20.575505671440073 29.120444384859727 29.053450329468163 21.250599614232033 80-81 20.71700690761907 29.424814504721503 28.78089376353851 21.077284824120916 82-83 20.73540539001289 29.182866111323552 28.875866052821518 21.205862445842037 84-85 20.629847438847694 29.071228779743226 28.72825674283102 21.57066703857806 86-87 20.59418414060088 29.21744331681337 28.78630747590091 21.402065066684848 88-89 20.711521497163528 28.766880223585844 28.80957352798174 21.712024751268885 90-91 20.455091397974446 28.723411674454287 29.056729510038664 21.764767417532603 92-93 20.694476405653997 28.950579834452554 28.990521661177088 21.36442209871636 94-95 20.390561255523796 29.021357511082456 28.942786541893284 21.645294691500467 96-97 20.1553715217297 29.104769623674244 28.94918114589984 21.79067770869622 98-99 20.35584202139221 29.090678034724853 28.570614943706644 21.982865000176286 100-101 20.694232688148052 28.515593527074852 29.058554747782754 21.73161903699434 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2034.0 1 1210.0 2 502.5 3 645.0 4 584.5 5 573.0 6 956.5 7 1776.5 8 2773.0 9 3243.0 10 2998.5 11 2809.0 12 2997.0 13 3218.5 14 3394.5 15 3518.5 16 3696.0 17 3930.5 18 4018.0 19 4224.5 20 4632.5 21 5146.0 22 5875.5 23 6750.0 24 7786.0 25 9337.0 26 11368.5 27 13736.5 28 15614.0 29 17349.5 30 19756.5 31 22317.0 32 25319.5 33 28473.0 34 32060.5 35 35387.0 36 38929.0 37 42320.5 38 43168.0 39 42582.0 40 42206.0 41 42491.5 42 42660.5 43 43988.5 44 45878.0 45 46079.5 46 45921.5 47 48993.0 48 57960.0 49 56466.0 50 45295.0 51 38695.0 52 34526.0 53 32077.0 54 30008.5 55 27018.5 56 23602.5 57 20844.5 58 18665.0 59 16657.5 60 14009.5 61 11562.5 62 9144.0 63 6258.5 64 4047.5 65 2961.5 66 2188.5 67 1598.5 68 1167.0 69 857.0 70 646.0 71 474.5 72 371.5 73 332.0 74 284.0 75 211.0 76 154.5 77 116.0 78 81.0 79 57.5 80 36.5 81 24.0 82 20.0 83 15.0 84 12.0 85 8.5 86 2.5 87 0.5 88 1.5 89 2.0 90 1.5 91 1.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30866107621375743 2 0.2754742086208497 3 0.2737723179750596 4 0.27671194727233345 5 0.28467988984126 6 0.1935513816257697 7 0.19231364297428596 8 0.1939381749543584 9 0.19084382832564906 10-11 0.19865705356314012 12-13 0.18863910635269363 14-15 0.18956741034130642 16-17 0.1964910109230436 18-19 0.19003156233561283 20-21 0.18225701643098058 22-23 0.18067116378376707 24-25 0.18430702107250055 26-27 0.18043908778661386 28-29 0.19502119627440667 30-31 0.19935328155459975 32-33 0.20167404152613175 34-35 0.19993347154748276 36-37 0.17347680787201783 38-39 0.1666305659559984 40-41 0.1727032212148405 42-43 0.18430702107250055 44-45 0.18921929634557663 46-47 0.1913466596528143 48-49 0.19119194232137884 50-51 0.19498251694154778 52-53 0.21861558931831546 54-55 0.18198626110096852 56-57 0.1654701859702324 58-59 0.16740415261317573 60-61 0.16063526936287403 62-63 0.1665145279574218 64-65 0.16376829532444226 66-67 0.16198904601293437 68-69 0.17235510721911068 70-71 0.16620509329455085 72-73 0.16322678466441812 74-75 0.1672107559488814 76-77 0.1728192592134171 78-79 0.17537209518210228 80-81 0.16620509329455085 82-83 0.1645032026487607 84-85 0.1585852647213541 86-87 0.15622582541696323 88-89 0.16086734536002723 90-91 0.16786830460748214 92-93 0.15881734071850728 94-95 0.1645418819816196 96-97 0.16214376334436983 98-99 0.16736547328031684 100-101 0.14605316087508122 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1292680.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.15383559920397 #Duplication Level Percentage of deduplicated Percentage of total 1 78.25660393387794 44.72665075788879 2 12.816336682993201 14.650055995276812 3 4.046010432110434 6.937350452085121 4 1.7013911143405873 3.889641121558736 5 0.8548722800359917 2.442961487574687 6 0.5053449851101708 1.7329442519921332 7 0.3422556649248881 1.369285680420931 8 0.2173120290365065 0.9936172785025548 9 0.17357411437548978 0.8928383757564754 >10 0.9621815898542567 10.288396373101147 >50 0.08062943074101526 3.1860196067700874 >100 0.037950505034790916 3.7932259913938338 >500 0.0029712006444656673 1.1125231778464224 >1k 0.0021608731959750307 2.1493240745947153 >5k 2.7010914949687884E-4 0.9735060236591329 >10k+ 1.3505457474843942E-4 0.8616593515784093 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11163 0.8635547854070613 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7339 0.5677352477024477 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5273 0.40791224432960976 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4686 0.3625027075533001 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2273 0.1758362471764087 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2195 0.1698022712504255 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1931 0.14937958350094377 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1896 0.14667203020082312 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1872 0.1448154222235975 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1659 0.1283380264257202 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1634 0.12640405978277688 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1500 0.11603799857660055 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1463 0.11317572794504441 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.73586657177337E-5 0.0 16-17 0.0 0.0 0.0 7.73586657177337E-5 0.0 18-19 0.0 0.0 0.0 1.1603799857660056E-4 0.0 20-21 0.0 0.0 0.0 1.9339666429433425E-4 0.0 22-23 0.0 0.0 0.0 2.7075533001206793E-4 0.0 24-25 0.0 0.0 0.0 4.2547266144753534E-4 0.0 26-27 0.0 0.0 0.0 8.122659900362038E-4 0.0 28-29 0.0 0.0 0.0 0.0018566079772256087 0.0 30-31 0.0 0.0 0.0 0.004486802611628555 0.0 32-33 0.0 0.0 0.0 0.007465111241761302 0.0 34-35 0.0 0.0 0.0 0.011565120524801189 0.0 36-37 0.0 0.0 0.0 0.017521737785066684 0.0 38-39 0.0 0.0 0.0 0.02688213633691246 0.0 40-41 0.0 0.0 0.0 0.03817650153170158 0.0 42-43 0.0 0.0 0.0 0.05353219667667172 0.0 44-45 0.0 0.0 0.0 0.06556146919577932 0.0 46-47 0.0 0.0 0.0 0.09050963888974843 0.0 48-49 0.0 0.0 0.0 0.12257480582974906 0.0 50-51 0.0 0.0 0.0 0.13843333230188445 0.0 52-53 0.0 0.0 0.0 0.16786830460748214 0.0 54-55 0.0 0.0 0.0 0.21033821208651793 0.0 56-57 0.0 0.0 0.0 0.28382894451836493 0.0 58-59 0.0 0.0 0.0 0.37762632670111707 0.0 60-61 0.0 0.0 0.0 0.48476807872017824 0.0 62-63 0.0 7.73586657177337E-5 0.0 0.6054089179069839 0.0 64-65 0.0 7.73586657177337E-5 0.0 0.7458922548503883 0.0 66-67 0.0 7.73586657177337E-5 0.0 0.9127548968035399 0.0 68-69 0.0 7.73586657177337E-5 0.0 1.1135779930067766 0.0 70-71 0.0 7.73586657177337E-5 0.0 1.2553764272673824 0.0 72-73 0.0 7.73586657177337E-5 0.0 1.423167373209147 0.0 74-75 0.0 7.73586657177337E-5 0.0 1.6181498901506948 0.0 76-77 0.0 7.73586657177337E-5 0.0 1.8387768047776714 0.0 78-79 0.0 7.73586657177337E-5 0.0 2.066868830646409 0.0 80-81 0.0 7.73586657177337E-5 0.0 2.3884874833678866 0.0 82-83 0.0 7.73586657177337E-5 0.0 2.731031655166012 0.0 84-85 0.0 7.73586657177337E-5 0.0 3.1601788532351396 0.0 86-87 0.0 7.73586657177337E-5 0.0 3.713680106445524 0.0 88-89 0.0 7.73586657177337E-5 0.0 4.307678621159142 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGCGC 115 2.5011104E-9 37.176296 8 TCGCGCA 115 2.5011104E-9 37.176296 9 CGTTCGC 125 2.1530468E-7 30.401947 6 TCGTTCG 135 4.1877502E-7 28.177263 5 GTTCGCG 145 7.8817357E-7 26.209593 7 GTACATG 11635 0.0 24.810318 1 TACATGG 11665 0.0 24.613695 2 CATGGGG 2700 0.0 24.301003 4 GTATCAA 17725 0.0 24.254492 1 GAGTACT 8125 0.0 23.94107 12-13 TACCTGG 1065 0.0 23.656523 2 ACATGGG 11790 0.0 23.506947 3 TAGCCCT 865 0.0 22.535988 4 AGTACTT 8495 0.0 22.367098 12-13 GTACTTT 8750 0.0 21.957426 14-15 ATCAACG 20355 0.0 20.97233 3 GTACCTG 1275 0.0 20.887566 1 TCAACGC 20445 0.0 20.83674 4 TATCAAC 20435 0.0 20.819632 2 CAACGCA 20580 0.0 20.701662 5 >>END_MODULE