FastQCFastQC Report
Thu 26 May 2016
SRR937627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937627_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292680
Sequences flagged as poor quality0
Sequence length101
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157731.2201782343658136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107130.8287433858340811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81930.6337995482253922No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47220.3652876195191385No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28100.2173778506668317No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23890.18480985239966582No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21880.16926076059040135No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20180.1561097874183866No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19920.15409846210972553No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19820.1533248754525482No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16290.1260172664541882No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13570.10497570937896464No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13360.10335117739889221No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGAC700.008393018527.142159
GTATCAA183050.025.781841
GTACATG106950.025.1962931
TACATGG107550.024.6522922
GATCGGA1551.4111829E-624.5154917
ACATGGG107650.023.5700633
ATGGGAT28250.023.5474075
CATGGGG24300.023.4643464
AGTACTT114100.023.04168712-13
GAGTACT112750.022.01162312-13
TCCGTAC652.9410952E-421.92504936-37
CATGGGA81150.021.7229544
TATCAAC217150.021.6843972
ATCAACG216550.021.6786523
TCAACGC217700.021.585964
AGGATCG1553.897211E-521.4585255
CAACGCA219000.021.4578235
ATCGGAC1553.9076906E-521.4510548
AACGCAG224000.020.9919456
GTACTTT117950.020.93972214-15
Produced by FastQC (version 0.11.5)