##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937627_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1292680 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15905637899557 33.0 31.0 34.0 31.0 34.0 2 32.424374941981 34.0 31.0 34.0 31.0 34.0 3 32.51048287279141 34.0 31.0 34.0 31.0 34.0 4 36.14678033233283 37.0 35.0 37.0 35.0 37.0 5 36.079142556549186 37.0 35.0 37.0 35.0 37.0 6 36.14361636290497 37.0 35.0 37.0 35.0 37.0 7 36.08692947984033 37.0 35.0 37.0 35.0 37.0 8 36.07563356747223 37.0 35.0 37.0 35.0 37.0 9 37.75168100380605 39.0 37.0 39.0 35.0 39.0 10-11 37.68195957236129 39.0 37.0 39.0 35.0 39.0 12-13 37.575037132159544 39.0 37.0 39.0 35.0 39.0 14-15 38.71178133799548 40.0 38.0 41.0 34.5 41.0 16-17 38.71116246866974 40.0 38.0 41.0 34.5 41.0 18-19 38.787710028777425 40.0 38.0 41.0 35.0 41.0 20-21 38.74054251632268 40.0 38.0 41.0 35.0 41.0 22-23 38.67892943342513 40.0 38.0 41.0 34.5 41.0 24-25 38.68404554878237 40.0 38.0 41.0 34.5 41.0 26-27 38.52294767459851 40.0 38.0 41.0 34.0 41.0 28-29 38.35694216666151 40.0 38.0 41.0 34.0 41.0 30-31 38.29579594331157 40.0 37.5 41.0 34.0 41.0 32-33 38.10306224278244 40.0 37.0 41.0 34.0 41.0 34-35 37.908162499613205 40.0 37.0 41.0 33.5 41.0 36-37 37.7165872451032 40.0 36.5 41.0 33.0 41.0 38-39 37.53609129869728 40.0 36.0 41.0 33.0 41.0 40-41 37.28443853080422 40.0 36.0 41.0 33.0 41.0 42-43 36.9560030324597 40.0 36.0 41.0 32.5 41.0 44-45 36.472524522697036 40.0 35.5 41.0 31.0 41.0 46-47 35.82751957174243 39.5 35.0 41.0 29.0 41.0 48-49 35.28186016647585 39.0 35.0 41.0 25.5 41.0 50-51 34.944188043444626 39.0 35.0 41.0 23.5 41.0 52-53 34.693805118049326 39.0 35.0 40.0 23.0 41.0 54-55 34.48190155336201 39.0 35.0 40.0 22.0 41.0 56-57 34.22151189776278 38.0 34.0 40.0 20.5 41.0 58-59 33.97559450134604 38.0 34.0 40.0 19.0 41.0 60-61 33.652206655939594 38.0 34.0 40.0 17.5 41.0 62-63 33.298174335489065 37.5 33.5 40.0 13.5 41.0 64-65 32.90852028344215 37.0 33.0 39.5 9.0 41.0 66-67 32.489316381471056 36.0 32.5 39.0 7.5 40.5 68-69 32.05729840331714 36.0 32.0 39.0 4.5 40.0 70-71 31.658543877835196 35.0 31.5 38.5 2.0 40.0 72-73 31.18347773617601 35.0 31.0 37.5 2.0 40.0 74-75 30.721373425751153 35.0 31.0 37.0 2.0 39.0 76-77 29.48749110375344 33.5 29.5 35.5 2.0 38.5 78-79 30.046199368753285 34.0 30.0 36.0 2.0 38.5 80-81 29.851385106909675 34.0 30.0 36.0 2.0 37.5 82-83 29.481882987282233 34.0 30.0 35.0 2.0 37.0 84-85 29.103643593155304 34.0 29.0 35.0 2.0 36.5 86-87 28.758690085713404 34.0 29.0 35.0 2.0 36.0 88-89 28.328287743293004 34.0 29.0 35.0 2.0 36.0 90-91 27.80229213726522 34.0 27.5 35.0 2.0 35.0 92-93 27.40170691895906 33.0 27.0 35.0 2.0 35.0 94-95 26.964076956400653 33.0 26.0 35.0 2.0 35.0 96-97 26.42875963115388 33.0 25.0 35.0 2.0 35.0 98-99 25.616408933378715 32.5 23.0 34.0 2.0 35.0 100-101 22.97619983290528 29.5 8.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3643555882187499 1101 2 0.3338556285495855 1101 3 0.3514371006543726 1101 4 0.06949975389811414 1101 5 -0.0010011430528038545 1101 6 0.11429953246565816 1101 7 0.1968326129763227 1101 8 0.12345364700355788 1101 9 0.19446116573845273 1101 10-11 0.2604952792153199 1101 12-13 0.23729552767110107 1101 14-15 0.28296945488625624 1101 16-17 0.3746421194009173 1101 18-19 0.3458131727141591 1101 20-21 0.4371901132182856 1101 22-23 0.3012418331834823 1101 24-25 0.2875855981442186 1101 26-27 0.2786469691036757 1101 28-29 0.25311828561452643 1101 30-31 0.22382746684509414 1101 32-33 0.4521138298603873 1101 34-35 0.2322438213748086 1101 36-37 0.26446321239313164 1101 38-39 0.3144782110429034 1101 40-41 0.2931943986076533 1101 42-43 0.31270199879887883 1101 44-45 0.3495612236552432 1101 46-47 0.28862491683493374 1101 48-49 0.2073277358775556 1101 50-51 0.327431031842103 1101 52-53 0.49343069960553976 1101 54-55 0.3968422007253949 1101 56-57 0.2740117950929246 1101 58-59 0.3609222267690697 1101 60-61 0.453160574793408 1101 62-63 0.3214585431291752 1101 64-65 0.2549657455936867 1101 66-67 0.07359831358636626 1101 68-69 0.26044100231428047 1101 70-71 0.2008341782438947 1101 72-73 0.17836492708119778 1101 74-75 0.29086433499370656 1101 76-77 0.4206682881866257 1101 78-79 0.36628484649980564 1101 80-81 0.40467511674734524 1101 82-83 0.3616001397359945 1101 84-85 0.35639199126665133 1101 86-87 0.07271595301394385 1101 88-89 0.1426394162507023 1101 90-91 -0.044244476783337205 1101 92-93 -0.05827299586828261 1101 94-95 -0.03240261340424766 1101 96-97 -0.044579756393222425 1101 98-99 -0.1461898663607002 1101 100-101 0.011026390197727665 1102 1 0.37782426846980854 1102 2 0.25486197031530367 1102 3 0.1792204182225987 1102 4 0.11594817870788177 1102 5 0.10946719704476493 1102 6 0.14416081507729217 1102 7 0.14813125376025482 1102 8 0.15569483912504722 1102 9 0.2272591844608698 1102 10-11 0.17411726151802043 1102 12-13 0.29323621515391807 1102 14-15 0.377986439262493 1102 16-17 0.37432701146043 1102 18-19 0.33593357772979004 1102 20-21 0.3114690344191686 1102 22-23 0.4134522950000914 1102 24-25 0.3116456329051118 1102 26-27 0.30823158577864973 1102 28-29 0.32980600607358923 1102 30-31 0.4396472833485987 1102 32-33 0.22551510846472667 1102 34-35 0.2577646522812529 1102 36-37 0.3533597637959147 1102 38-39 0.5201947699942977 1102 40-41 0.5788569290700281 1102 42-43 0.4806667626251837 1102 44-45 0.3732700382902294 1102 46-47 0.2918225926080851 1102 48-49 0.37432446015287013 1102 50-51 0.3740180808474989 1102 52-53 0.5792449012466605 1102 54-55 0.4078820452799192 1102 56-57 0.565979979092667 1102 58-59 0.387491475961788 1102 60-61 0.4211774100612615 1102 62-63 0.31717691933893377 1102 64-65 0.4867698637807152 1102 66-67 0.6135878992980608 1102 68-69 0.5626475733341323 1102 70-71 0.4948840549965041 1102 72-73 0.4360568225748622 1102 74-75 0.3428903450864773 1102 76-77 0.2301706855697745 1102 78-79 0.39441740384828705 1102 80-81 0.49005538970886775 1102 82-83 0.5885733794416943 1102 84-85 0.5678591245182893 1102 86-87 0.20441068622643854 1102 88-89 0.5571397400954616 1102 90-91 0.4533732838883999 1102 92-93 0.4878370228168585 1102 94-95 0.2040326013351148 1102 96-97 0.3849001349440435 1102 98-99 0.4625625624749965 1102 100-101 0.4412681321563596 1103 1 0.36076950286582843 1103 2 0.27483000359642773 1103 3 0.26290973489410874 1103 4 0.16778722213094 1103 5 0.017980808422620953 1103 6 0.14860808115715685 1103 7 0.12290508869245542 1103 8 0.14793339209398226 1103 9 0.09525503282845449 1103 10-11 0.24493986749683927 1103 12-13 0.24834987991598467 1103 14-15 0.33597322720277134 1103 16-17 0.2620077547557642 1103 18-19 0.18051428306065986 1103 20-21 0.16762704104009174 1103 22-23 0.29636036250239783 1103 24-25 0.2329608198615034 1103 26-27 0.32400305670499563 1103 28-29 0.32900152094938306 1103 30-31 0.556730708402803 1103 32-33 0.46546143713066357 1103 34-35 0.5884745027310316 1103 36-37 0.6585879600158435 1103 38-39 0.5967447464393203 1103 40-41 0.5525053887442155 1103 42-43 0.5702642329368715 1103 44-45 0.6867773063055367 1103 46-47 0.7839066823063163 1103 48-49 0.681135572575549 1103 50-51 0.7122447893282526 1103 52-53 0.8984885788868695 1103 54-55 0.8690926225843896 1103 56-57 0.8779037991900083 1103 58-59 0.7125550425160512 1103 60-61 0.8293812973248791 1103 62-63 0.8804730964128638 1103 64-65 0.9268008946808095 1103 66-67 0.9433151400257387 1103 68-69 1.1403798185654495 1103 70-71 0.9267819699284416 1103 72-73 0.7336012021855005 1103 74-75 0.8265486095892776 1103 76-77 0.8481091086938584 1103 78-79 0.8915168973363663 1103 80-81 0.9346631593443142 1103 82-83 0.8718922501211068 1103 84-85 0.9492185714493857 1103 86-87 0.7197355163700117 1103 88-89 0.8265909461850889 1103 90-91 1.0265920644952544 1103 92-93 0.9017483412260106 1103 94-95 0.8455640262361861 1103 96-97 0.7373146005755693 1103 98-99 0.795091676541734 1103 100-101 0.7623970061005068 1104 1 0.30105485065141124 1104 2 0.21926606069473564 1104 3 0.09606847449165912 1104 4 0.061499690896631876 1104 5 0.10566486359834926 1104 6 0.10816138821927268 1104 7 0.07749993365835905 1104 8 0.07461737435114912 1104 9 0.1622707842644857 1104 10-11 0.15784224186894136 1104 12-13 0.14158221590827935 1104 14-15 0.22397981862980032 1104 16-17 0.20732987506317357 1104 18-19 0.14633473497086413 1104 20-21 0.11962608796369523 1104 22-23 0.06765540640245149 1104 24-25 0.07076375283085667 1104 26-27 0.033695525244745284 1104 28-29 0.04266240240126962 1104 30-31 0.18266106193458143 1104 32-33 0.1026946810103837 1104 34-35 0.19353166163905655 1104 36-37 0.07296796225475077 1104 38-39 0.20284690020709917 1104 40-41 0.14645607525705628 1104 42-43 0.2590067369602238 1104 44-45 0.1649381975141253 1104 46-47 0.2698077246309154 1104 48-49 0.34956820352854834 1104 50-51 0.5069965016306384 1104 52-53 0.6454791477440693 1104 54-55 0.4890279920914935 1104 56-57 0.5749484921053067 1104 58-59 0.41530150468209825 1104 60-61 0.3850610369542622 1104 62-63 0.5698176920691793 1104 64-65 0.6048613431402217 1104 66-67 0.538818683765065 1104 68-69 0.5514302155607851 1104 70-71 0.5531069566541902 1104 72-73 0.5908536205654933 1104 74-75 0.4991663550911767 1104 76-77 0.3139426551342517 1104 78-79 0.39169919373696516 1104 80-81 0.3826555367872153 1104 82-83 0.23245835490405042 1104 84-85 0.34972170951503045 1104 86-87 0.4228483686257114 1104 88-89 0.49555120967784916 1104 90-91 0.5986890071535385 1104 92-93 0.6193388655104606 1104 94-95 0.7178533301104224 1104 96-97 0.6111621535416596 1104 98-99 0.8704774003200662 1104 100-101 0.3830310073062293 1105 1 0.27073107919877515 1105 2 0.22944054283128423 1105 3 0.21288627159011497 1105 4 0.14932190269910706 1105 5 0.14691015981266986 1105 6 0.1901125602417295 1105 7 0.12735509480896212 1105 8 0.13812593156547592 1105 9 0.15728288397083645 1105 10-11 0.1876380171044758 1105 12-13 0.24024250622175458 1105 14-15 0.23753238380729158 1105 16-17 0.1354304993206057 1105 18-19 0.29235126924939436 1105 20-21 0.1285240411463775 1105 22-23 0.11410814602233899 1105 24-25 0.09123093262573434 1105 26-27 0.11696296253091276 1105 28-29 0.0926864428687253 1105 30-31 0.2077942779133437 1105 32-33 0.1895801093082028 1105 34-35 0.21072307939085988 1105 36-37 0.09945767651094428 1105 38-39 0.050783492977480194 1105 40-41 0.1746013035222873 1105 42-43 0.2745977748329054 1105 44-45 0.1505352753059057 1105 46-47 0.4002267638864154 1105 48-49 0.13513170413850162 1105 50-51 0.13651998380294117 1105 52-53 0.14571359197217504 1105 54-55 0.0866317932668963 1105 56-57 0.1997145445810773 1105 58-59 0.20662378999674758 1105 60-61 0.034918085579583646 1105 62-63 -0.06643215395262558 1105 64-65 0.035201108457577845 1105 66-67 0.16418028418468822 1105 68-69 0.30690836257718956 1105 70-71 0.1258691070243323 1105 72-73 0.055718775398027276 1105 74-75 -0.015133749415316089 1105 76-77 0.04205788248554754 1105 78-79 0.18932663801672334 1105 80-81 0.09327735043186891 1105 82-83 0.004131531702814328 1105 84-85 -0.0735340651641998 1105 86-87 -0.1421180144561589 1105 88-89 0.10637279762026353 1105 90-91 0.17313995069221377 1105 92-93 0.4363481916223826 1105 94-95 0.1142125900613884 1105 96-97 0.14251658553098068 1105 98-99 0.246852276640805 1105 100-101 0.09599229619317384 1106 1 0.09007442131223087 1106 2 0.02596538104417334 1106 3 0.050339671012814335 1106 4 0.0016811752672509783 1106 5 -0.008201387031952834 1106 6 -0.09796947360522523 1106 7 0.002278303789935876 1106 8 -0.12552183118604177 1106 9 -0.07527398346051939 1106 10-11 -0.17835744696574096 1106 12-13 -0.37933794131209453 1106 14-15 -0.1968260219690805 1106 16-17 -0.13505271605611568 1106 18-19 -0.01880699178116174 1106 20-21 -0.04008694607055219 1106 22-23 -0.014650406182930453 1106 24-25 -0.048382503488340944 1106 26-27 -0.05208773216482143 1106 28-29 -0.1432513332633647 1106 30-31 -0.01296096701845073 1106 32-33 -0.13008617585787619 1106 34-35 -0.39298371777145036 1106 36-37 -0.14375631165692226 1106 38-39 -0.11532179276927224 1106 40-41 -0.018185179575269217 1106 42-43 -0.3119751577003953 1106 44-45 -0.06428602252542959 1106 46-47 -0.08668720492591575 1106 48-49 -0.11358704296868893 1106 50-51 0.0765125361260246 1106 52-53 0.026959962807573845 1106 54-55 0.14647408765631553 1106 56-57 0.08879254742549847 1106 58-59 -0.008934912003603301 1106 60-61 0.05346364264057257 1106 62-63 -0.05933477031731371 1106 64-65 -0.30296024104464436 1106 66-67 -0.288165102884129 1106 68-69 0.1460722030538406 1106 70-71 -0.033216638037899315 1106 72-73 0.1087224613669413 1106 74-75 0.07160118061018395 1106 76-77 -0.04878904881910273 1106 78-79 0.025748092375508236 1106 80-81 -0.21482820460940744 1106 82-83 -0.3509690750514274 1106 84-85 -0.6648501580371509 1106 86-87 -0.12718373915298997 1106 88-89 -0.1435083340900114 1106 90-91 -0.37778827389942293 1106 92-93 -0.105660703512644 1106 94-95 -0.08551453883812243 1106 96-97 -0.11968792683553175 1106 98-99 -0.4771544893843256 1106 100-101 -0.9016092993769291 1107 1 0.11978745578117866 1107 2 0.07719647710275268 1107 3 0.0718163768296094 1107 4 0.00662645504993975 1107 5 0.014437633663348493 1107 6 0.05875584570487291 1107 7 0.03371522100002977 1107 8 0.016243581457509038 1107 9 0.06200541614072819 1107 10-11 0.07810384838413142 1107 12-13 0.017313982384330018 1107 14-15 0.04133316728363923 1107 16-17 -0.013227315775765192 1107 18-19 0.09186540955266764 1107 20-21 0.11676788210710498 1107 22-23 0.07732047935299136 1107 24-25 0.12158863453546331 1107 26-27 0.0249014805083263 1107 28-29 0.08309969453642196 1107 30-31 0.028518873130607858 1107 32-33 0.0627568811608441 1107 34-35 -0.11327068330250256 1107 36-37 0.08247064882437627 1107 38-39 0.12202987060344839 1107 40-41 0.06892531434325377 1107 42-43 0.14626639483884674 1107 44-45 0.34281911690882083 1107 46-47 0.2749157012966137 1107 48-49 0.16951716487236723 1107 50-51 0.19535596752333362 1107 52-53 0.4229686954054941 1107 54-55 0.3368575645014289 1107 56-57 0.2608423571889418 1107 58-59 0.47739374014646074 1107 60-61 0.33469813422576067 1107 62-63 0.23175545491928773 1107 64-65 0.2846682883440721 1107 66-67 0.2972889835015522 1107 68-69 0.4358357786304339 1107 70-71 0.3266319127687538 1107 72-73 0.3465823654215434 1107 74-75 0.17270189715336315 1107 76-77 0.3338606808447402 1107 78-79 0.28044088428196545 1107 80-81 0.3645520247192344 1107 82-83 0.4819557793327185 1107 84-85 0.5414095823356639 1107 86-87 0.5200700198690953 1107 88-89 0.4696367071351659 1107 90-91 0.5534109564478769 1107 92-93 0.4901399442493535 1107 94-95 0.6756765509517884 1107 96-97 0.7939187754116119 1107 98-99 0.7383863570595146 1107 100-101 0.9836545504537284 1108 1 0.1391528697803679 1108 2 -0.00640494727193186 1108 3 0.013540691129016125 1108 4 0.017022907437187484 1108 5 -0.03351842734321764 1108 6 0.021601865815462418 1108 7 0.02209543534392111 1108 8 -0.10613218869982433 1108 9 0.09732627652493164 1108 10-11 -0.0837573266605176 1108 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96-97 -0.14888957949070303 2316 98-99 0.08908700241651601 2316 100-101 0.09750269340104012 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 10.0 5 20.0 6 69.0 7 156.0 8 365.0 9 657.0 10 1265.0 11 2498.0 12 4133.0 13 6313.0 14 8007.0 15 12025.0 16 17443.0 17 26099.0 18 21940.0 19 10325.0 20 7269.0 21 7003.0 22 6965.0 23 7201.0 24 7949.0 25 9441.0 26 11379.0 27 13632.0 28 16905.0 29 21248.0 30 26482.0 31 33471.0 32 43309.0 33 57529.0 34 80877.0 35 117899.0 36 181927.0 37 282780.0 38 228651.0 39 19434.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.50418469770908 19.979697426664302 15.47258804422928 20.04352983139734 2 15.013769842497757 21.970170498499243 40.93704551783891 22.079014141164095 3 20.17807964848222 27.402914874524242 30.027694402326947 22.391311074666582 4 12.191416282451959 17.09030850635888 38.256180957390846 32.46209425379831 5 12.219300458591352 39.48180570151476 33.12598957760997 15.172904262283918 6 29.28172478881084 37.4768697589504 17.701519324194695 15.539886128044063 7 26.686650988643745 35.606491939227034 20.323823374694435 17.383033697434787 8 26.45650895813349 33.71808955039143 19.85619024043073 19.969211251044342 9 27.54370764613052 17.117925550020114 21.232168827552062 34.1061979762973 10-11 24.446808181452486 28.184546832936224 26.268063248445088 21.100581737166195 12-13 26.51856510755427 25.861221319538163 27.824394749759502 19.79581882314806 14-15 23.122466503697744 25.983422037936688 28.816528452517247 22.077583005848314 16-17 19.80172973976545 29.687548349166075 29.106468731627317 21.40425317944116 18-19 19.632507658507905 27.172115295355386 33.951209889531825 19.24416715660488 20-21 21.126458210848778 25.506003032459695 34.06074976018814 19.30678899650339 22-23 20.352301961174142 25.862875236090893 33.99998994329177 19.784832859443192 24-25 20.13462770253335 25.90347465258116 34.548332296724006 19.413565348161487 26-27 20.048396255169564 25.86020166137784 34.734756649456706 19.356645433995894 28-29 19.48800326766922 26.54900014659643 34.67516126574219 19.287835319992155 30-31 19.761077760930778 26.249651886004273 34.43272890429186 19.55654144877309 32-33 19.512563047312558 26.30086332270941 34.38809295417273 19.798480675805305 34-35 20.045409536776308 26.133111056100507 34.332820187517406 19.48865921960578 36-37 20.1720843518891 25.864134975399942 34.31305504842653 19.650725624284433 38-39 19.923414920939443 26.332696413652258 34.33239471485596 19.41149395055234 40-41 19.985332689188777 26.547020296355488 34.02402236300743 19.4436246514483 42-43 19.699614247318067 26.751283134422355 33.40122302013558 20.147879598124003 44-45 20.06861713649163 26.673500015471735 32.95463687842312 20.303245969613513 46-47 20.535360646099576 27.193040814432035 32.06826128662933 20.203337252839063 48-49 20.59771173066807 27.440781941393077 31.511665686790234 20.449840641148622 50-51 20.69061948819507 27.843665872451034 31.221686728347308 20.24402791100659 52-53 20.596860785345175 28.462960670854347 30.42558869944611 20.514589844354365 54-55 20.356422867875835 28.040161587030955 30.643052639559116 20.960362905534094 56-57 20.868505740012996 28.28871027632515 30.08532660828666 20.75745737537519 58-59 21.017382492186776 27.74762508896246 30.45428876442739 20.78070365442337 60-61 20.81818392796361 27.930268898722034 30.909157718847663 20.342389454466687 62-63 20.848121731596372 27.802549741622055 31.00013150973172 20.34919701704985 64-65 20.58746170746047 28.061275799115016 30.934067209208777 20.41719528421574 66-67 20.650470340687562 28.06858619302534 30.354264009654365 20.926679456632733 68-69 20.716534641210508 28.06920506235108 30.389346164557352 20.82491413188105 70-71 20.87078782065167 28.187254386236347 30.200281585543216 20.741676207568773 72-73 20.771227217872944 27.84413002444534 30.333261131912 21.051381625769718 74-75 20.55682767583625 28.69991026394777 29.70874462357273 21.034517436643252 76-77 20.44543119720271 28.157626017266452 30.243989231673734 21.152953553857103 78-79 20.453476498437357 28.10316551660117 30.1563805427484 21.286977442213075 80-81 20.5714484636569 28.40966055017483 29.897654485255437 21.121236500912833 82-83 20.577270747577302 28.265820561953603 29.970858742647966 21.186049947821136 84-85 20.399681436891356 28.10565186498277 29.876276370128867 21.618390327997 86-87 20.26046662747161 28.182883621623294 29.826445833462262 21.730203917442832 88-89 20.273656279976482 27.843124361791006 29.91266206640468 21.97055729182783 90-91 20.140523285411664 27.68037161341435 30.08496540881963 22.09413969235435 92-93 20.102664114826613 27.82053622679401 30.216908983168068 21.859890675211307 94-95 19.895759197945353 27.854109292322924 30.1884457096884 22.06168580004332 96-97 19.66789924807377 27.865094222854843 30.22735711854442 22.23964941052697 98-99 19.790355583233087 27.865030769766335 29.819404099283265 22.525209547717314 100-101 19.574528995318968 27.57468374018337 30.314325505822275 22.536461758675383 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 813.0 1 593.5 2 619.0 3 841.0 4 638.0 5 559.5 6 1053.0 7 2344.5 8 3689.0 9 4039.5 10 3654.0 11 3319.5 12 3429.5 13 3754.5 14 3999.5 15 4207.0 16 4390.0 17 4587.5 18 4825.0 19 5073.0 20 5435.0 21 6016.5 22 6696.0 23 7466.5 24 8412.5 25 9698.5 26 11568.5 27 14025.5 28 15883.5 29 17617.5 30 19895.0 31 22261.0 32 25272.0 33 28188.0 34 31235.0 35 34384.5 36 38243.0 37 41665.0 38 42524.5 39 42183.0 40 41986.0 41 42011.5 42 41621.5 43 42978.5 44 45236.5 45 45793.0 46 45278.0 47 48075.0 48 58614.5 49 56763.5 50 44459.5 51 38452.0 52 34368.0 53 32218.5 54 30125.0 55 26871.5 56 23308.0 57 20576.5 58 18479.5 59 16289.0 60 13540.5 61 11105.5 62 8840.0 63 5989.0 64 3835.5 65 2795.5 66 2033.0 67 1500.5 68 1117.0 69 809.5 70 616.0 71 477.0 72 386.5 73 366.5 74 309.5 75 238.5 76 185.0 77 112.5 78 64.0 79 41.5 80 35.0 81 31.0 82 23.0 83 14.0 84 8.5 85 4.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018024569112231954 2 0.0 3 0.0 4 0.0 5 0.03573970356159297 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004138688615898754 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.122659900362039E-4 24-25 3.094346628709348E-4 26-27 0.0013924559829192067 28-29 0.0011990593186248725 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.349073243184701E-4 42-43 0.010984930531918185 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.547173314354674E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.509453228950707E-4 84-85 3.867933285886685E-5 86-87 0.0 88-89 0.0 90-91 0.01191323452053099 92-93 0.0013924559829192067 94-95 0.0 96-97 0.0 98-99 0.0011603799857660055 100-101 0.01779249311507875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1292680.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.78606622046425 #Duplication Level Percentage of deduplicated Percentage of total 1 77.30852027811082 42.354297113626814 2 13.143650647124765 14.401778294640504 3 4.279042899588494 7.032957829711877 4 1.8276365874703167 4.005160764323682 5 0.924475596827129 2.5324190633487147 6 0.5557774301224813 1.8269315454317818 7 0.36295817987238527 1.3919535614423368 8 0.2578550342054567 1.1301490383408177 9 0.16892722771769347 0.8329372455762898 >10 1.0372724372846165 10.620492064645719 >50 0.08584451578813931 3.2959208653082106 >100 0.04222913331521938 4.332423017775373 >500 0.0028341622304604774 1.1115189045053757 >1k 0.0025507460074144298 2.438711449907303 >5k 1.4170811152302388E-4 0.6360742044152087 >10k+ 2.8341622304604776E-4 2.0562750370000265 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15773 1.2201782343658136 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10713 0.8287433858340811 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8193 0.6337995482253922 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4722 0.3652876195191385 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2810 0.2173778506668317 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2389 0.18480985239966582 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2188 0.16926076059040135 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2018 0.1561097874183866 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1992 0.15409846210972553 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1982 0.1533248754525482 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1629 0.1260172664541882 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1357 0.10497570937896464 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1336 0.10335117739889221 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.73586657177337E-5 0.0 16-17 0.0 0.0 0.0 1.1603799857660056E-4 0.0 18-19 0.0 0.0 0.0 1.9339666429433425E-4 0.0 20-21 0.0 0.0 0.0 2.7075533001206793E-4 0.0 22-23 0.0 0.0 0.0 3.4811399572980166E-4 0.0 24-25 0.0 0.0 0.0 5.801899928830028E-4 0.0 26-27 0.0 0.0 0.0 9.669833214716713E-4 0.0 28-29 0.0 0.0 0.0 0.0020500046415199433 0.0 30-31 0.0 0.0 0.0 0.004641519943064022 0.0 32-33 0.0 0.0 0.0 0.00773586657177337 0.0 34-35 0.0 0.0 0.0 0.01191323452053099 0.0 36-37 0.0 0.0 0.0 0.017985889779373085 0.0 38-39 0.0 0.0 0.0 0.027230250332642265 0.0 40-41 0.0 0.0 0.0 0.038640653526007984 0.0 42-43 0.0 0.0 0.0 0.05426710400099019 0.0 44-45 0.0 0.0 0.0 0.06652845251725098 0.0 46-47 0.0 0.0 0.0 0.0915926602097967 0.0 48-49 0.0 0.0 0.0 0.12404462047838599 0.0 50-51 0.0 0.0 0.0 0.14021258161339234 0.0 52-53 0.0 0.0 0.0 0.1703437819104496 0.0 54-55 0.0 0.0 0.0 0.21323916205093293 0.0 56-57 0.0 0.0 0.0 0.2872714051428041 0.0 58-59 0.0 0.0 0.0 0.3815716186527215 0.0 60-61 0.0 0.0 0.0 0.48944827799610113 0.0 62-63 0.0 0.0 0.0 0.6103211931800601 0.0 64-65 0.0 0.0 0.0 0.7509979267877588 0.0 66-67 0.0 0.0 0.0 0.9184794380666522 0.0 68-69 0.0 0.0 0.0 1.1218166909057152 0.0 70-71 0.0 0.0 0.0 1.2642339944920629 0.0 72-73 0.0 0.0 0.0 1.4338428690781941 0.0 74-75 0.0 0.0 0.0 1.6301791626698023 0.0 76-77 0.0 0.0 0.0 1.8532041959340284 0.0 78-79 0.0 0.0 0.0 2.0829207537828385 0.0 80-81 0.0 0.0 0.0 2.408252622458768 0.0 82-83 0.0 0.0 0.0 2.7540071788841787 0.0 84-85 0.0 0.0 0.0 3.188376086889253 0.0 86-87 0.0 0.0 0.0 3.752320759971532 0.0 88-89 0.0 0.0 0.0 4.355486276572702 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGCGAC 70 0.0083930185 27.14215 9 GTATCAA 18305 0.0 25.78184 1 GTACATG 10695 0.0 25.196293 1 TACATGG 10755 0.0 24.652292 2 GATCGGA 155 1.4111829E-6 24.515491 7 ACATGGG 10765 0.0 23.570063 3 ATGGGAT 2825 0.0 23.547407 5 CATGGGG 2430 0.0 23.464346 4 AGTACTT 11410 0.0 23.041687 12-13 GAGTACT 11275 0.0 22.011623 12-13 TCCGTAC 65 2.9410952E-4 21.925049 36-37 CATGGGA 8115 0.0 21.722954 4 TATCAAC 21715 0.0 21.684397 2 ATCAACG 21655 0.0 21.678652 3 TCAACGC 21770 0.0 21.58596 4 AGGATCG 155 3.897211E-5 21.458525 5 CAACGCA 21900 0.0 21.457823 5 ATCGGAC 155 3.9076906E-5 21.451054 8 AACGCAG 22400 0.0 20.991945 6 GTACTTT 11795 0.0 20.939722 14-15 >>END_MODULE