FastQCFastQC Report
Thu 26 May 2016
SRR937626_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937626_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300193
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114680.8820228996772018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75070.5773758203589775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53910.4146307509731248No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48400.3722524271396631No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22580.17366652489284284No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22010.1692825603583468No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19350.1488240591973653No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18580.14290186149287068No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18090.1391331902263741No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16720.12859629301188363No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15900.12228953701488932No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14730.11329087297039747No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14230.10944529004540095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG651.0034069E-525.57473676-77
GTATCAA175750.024.8876761
GTACATG119850.024.7536031
TACATGG118050.024.5577282
GAGTACT83500.023.77824212-13
ACATGGG118750.023.3724143
CGCGAAT853.155972E-622.34930478-79
AGTACTT87500.022.09409712-13
GTACTTT90450.021.68817514-15
TATCAAC201950.021.5799182
CATGGGA87900.021.5778834
ATCAACG201950.021.5563853
TCAACGC202150.021.5400414
CAACGCA203650.021.3572225
AACGCAG208300.020.8683786
TATTCCG1150.00370794320.6671355
CGGGTCG2309.008909E-820.6551846
ATGGGAG29250.020.6388725
ATGGGAT32100.020.4353525
GGTATCA130800.019.9915641
Produced by FastQC (version 0.11.5)