##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937626_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1300193 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.893235081253323 33.0 31.0 34.0 30.0 34.0 2 31.992274993020267 33.0 31.0 34.0 30.0 34.0 3 32.03349425816013 34.0 31.0 34.0 30.0 34.0 4 35.619644160520785 37.0 35.0 37.0 33.0 37.0 5 35.617593695705175 37.0 35.0 37.0 33.0 37.0 6 35.73371722505813 37.0 35.0 37.0 35.0 37.0 7 35.68361081777859 37.0 35.0 37.0 33.0 37.0 8 35.68587432788824 37.0 35.0 37.0 33.0 37.0 9 37.30288810968833 39.0 37.0 39.0 34.0 39.0 10-11 37.20887514392094 39.0 37.0 39.0 34.0 39.0 12-13 37.09810620423276 39.0 37.0 39.0 33.5 39.0 14-15 38.20805411196645 40.0 38.0 41.0 33.5 41.0 16-17 38.19033674231441 40.0 38.0 41.0 33.0 41.0 18-19 38.22518233831439 40.0 38.0 41.0 33.5 41.0 20-21 38.08100758887335 40.0 38.0 41.0 33.0 41.0 22-23 37.96110423606342 40.0 38.0 41.0 33.0 41.0 24-25 37.831388878420356 40.0 37.5 41.0 33.0 41.0 26-27 37.901761123156334 40.0 38.0 41.0 33.0 41.0 28-29 37.84666468747332 40.0 37.5 41.0 33.0 41.0 30-31 37.61306821371904 40.0 37.0 41.0 33.0 41.0 32-33 37.331039314932475 39.5 36.0 41.0 33.0 41.0 34-35 37.0618338969676 39.0 36.0 41.0 31.5 41.0 36-37 37.040581282932614 39.0 36.0 41.0 31.5 41.0 38-39 36.96636345527165 39.0 36.0 41.0 31.0 41.0 40-41 36.75606659934333 39.5 35.5 41.0 31.0 41.0 42-43 36.45246705681387 39.0 35.0 41.0 30.0 41.0 44-45 36.10369345166448 39.0 35.0 41.0 30.0 41.0 46-47 35.583606433814055 39.0 35.0 41.0 27.5 41.0 48-49 35.129961090391966 39.0 35.0 40.5 25.0 41.0 50-51 34.17815855030753 38.0 34.0 39.5 23.5 40.5 52-53 34.24630266429676 38.0 34.5 39.5 23.0 40.5 54-55 34.56582176646083 39.0 35.0 40.0 23.0 41.0 56-57 34.333482029206436 38.0 34.0 40.0 21.5 41.0 58-59 34.13345364880445 38.0 34.0 40.0 21.0 41.0 60-61 33.832724449370204 38.0 34.0 40.0 19.5 41.0 62-63 33.501612837478746 38.0 33.5 40.0 18.0 41.0 64-65 33.14758155135429 37.0 33.0 40.0 15.0 41.0 66-67 32.74485057218428 36.5 32.5 39.0 10.5 41.0 68-69 32.28567604963263 36.0 32.0 39.0 7.5 40.5 70-71 31.854000521461046 35.5 31.5 39.0 6.5 40.0 72-73 31.393299302488167 35.0 31.0 38.0 2.0 40.0 74-75 30.91811715645293 35.0 31.0 37.0 2.0 39.5 76-77 30.475045243283112 35.0 30.5 37.0 2.0 39.0 78-79 29.961611853009515 34.5 30.0 36.0 2.0 39.0 80-81 29.459236820995038 34.0 29.0 36.0 2.0 37.5 82-83 28.961605700076834 34.0 29.0 35.0 2.0 37.0 84-85 28.50641327864402 34.0 29.0 35.0 2.0 36.5 86-87 28.01908601261505 34.0 27.5 35.0 2.0 36.0 88-89 27.556747728991006 33.5 26.5 35.0 2.0 36.0 90-91 27.07345947870816 33.0 25.5 35.0 2.0 35.0 92-93 26.56015299267109 33.0 25.0 35.0 2.0 35.0 94-95 25.963333905043328 32.5 23.5 35.0 2.0 35.0 96-97 25.244874030240126 32.0 19.5 34.5 2.0 35.0 98-99 24.405064478888903 32.0 8.5 34.0 2.0 35.0 100-101 21.937718477179928 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1005928419146791 1101 2 0.2566627832543631 1101 3 0.28950156476014755 1101 4 0.16277946293162415 1101 5 0.16327557558345518 1101 6 0.094576252067192 1101 7 0.09720398242350825 1101 8 0.11683338704314394 1101 9 0.08726003264163751 1101 10-11 0.20997679780455059 1101 12-13 0.14656216217525753 1101 14-15 0.30562981683739565 1101 16-17 0.43237448412410373 1101 18-19 0.27464187179511157 1101 20-21 0.3261032883862214 1101 22-23 0.3441336144636864 1101 24-25 0.2773917349766677 1101 26-27 0.3120157449549126 1101 28-29 0.2563164743834463 1101 30-31 0.3136102081109797 1101 32-33 0.22235596653927558 1101 34-35 0.35621736375524193 1101 36-37 0.24652059257780223 1101 38-39 0.39050079238361235 1101 40-41 0.4603102065452802 1101 42-43 0.6645283192323745 1101 44-45 0.6246402232221087 1101 46-47 0.6953470082566469 1101 48-49 0.8310478604494378 1101 50-51 0.7600305568436454 1101 52-53 0.745394055953831 1101 54-55 0.8302010563788187 1101 56-57 0.9213719691882929 1101 58-59 0.9636633716397398 1101 60-61 1.043116466856297 1101 62-63 0.9786553376764147 1101 64-65 0.8645423579819962 1101 66-67 0.9057402183428636 1101 68-69 0.9365257463484937 1101 70-71 1.0030153545687206 1101 72-73 0.8690892893714697 1101 74-75 0.8409150448865752 1101 76-77 0.8036918900129244 1101 78-79 0.9106570032222656 1101 80-81 1.1165663843576041 1101 82-83 1.2580113091849086 1101 84-85 1.3789214067347793 1101 86-87 1.24821698649302 1101 88-89 1.2402497444532479 1101 90-91 1.2285868859847184 1101 92-93 1.38286351616091 1101 94-95 1.6121276780881892 1101 96-97 1.853855162989266 1101 98-99 2.173244737920424 1101 100-101 2.34085737958598 1102 1 0.14250453333299617 1102 2 0.2442260467071904 1102 3 0.20168303258299503 1102 4 0.17212916447209636 1102 5 0.14829999499938396 1102 6 0.2034115400612606 1102 7 0.22649122341711347 1102 8 0.08427139716529553 1102 9 0.04811698248492746 1102 10-11 0.14457872256395632 1102 12-13 0.22244923483548007 1102 14-15 0.30924566232506834 1102 16-17 0.2676998548451692 1102 18-19 0.2908204270073611 1102 20-21 0.37465833451178554 1102 22-23 0.35455062721051434 1102 24-25 0.3103870172119585 1102 26-27 0.41367056183698026 1102 28-29 0.24959144475898398 1102 30-31 0.20539094279762793 1102 32-33 0.19734129274058176 1102 34-35 0.3845779405185894 1102 36-37 0.3257563368439449 1102 38-39 0.4350343870766764 1102 40-41 0.3284951960798139 1102 42-43 0.41139975460674805 1102 44-45 0.5619388045703246 1102 46-47 0.5799306599837308 1102 48-49 0.896188452052705 1102 50-51 0.8557909364797354 1102 52-53 0.8109291789368811 1102 54-55 0.8112580128668512 1102 56-57 0.8236361640120435 1102 58-59 0.8331551598497597 1102 60-61 0.8831194569888581 1102 62-63 0.8336297554311543 1102 64-65 0.6614583684806803 1102 66-67 0.8381659910706389 1102 68-69 0.8315776306857288 1102 70-71 1.0170316009556366 1102 72-73 0.7826453875807111 1102 74-75 0.7346476716626604 1102 76-77 0.862143444328126 1102 78-79 0.8590799851596493 1102 80-81 0.8557050046882893 1102 82-83 0.752263609925798 1102 84-85 0.8645507742663661 1102 86-87 1.1141746041325895 1102 88-89 1.0682520590373414 1102 90-91 1.2598379242251951 1102 92-93 1.430903595011813 1102 94-95 1.4702901862556779 1102 96-97 1.5749187088435015 1102 98-99 1.7090944892964366 1102 100-101 2.118682224265207 1103 1 0.17097193245833608 1103 2 0.3116016979847416 1103 3 0.19021008643555248 1103 4 0.03766746035750401 1103 5 0.11652709120730265 1103 6 0.12722631272860951 1103 7 0.20459018188334 1103 8 0.16567152270047814 1103 9 0.2230463476817448 1103 10-11 0.2971086477361453 1103 12-13 0.2601737905719119 1103 14-15 0.35811082767258995 1103 16-17 0.3056468187818666 1103 18-19 0.38756735624328087 1103 20-21 0.4498801473555005 1103 22-23 0.4133865265306156 1103 24-25 0.3231865453493583 1103 26-27 0.23422010145625904 1103 28-29 0.2499745992301996 1103 30-31 0.2716556162105306 1103 32-33 0.4081647048821182 1103 34-35 0.5354950872977966 1103 36-37 0.5584059456946875 1103 38-39 0.6002028814879807 1103 40-41 0.7990969144489526 1103 42-43 0.6129645217457877 1103 44-45 0.8274384873303333 1103 46-47 1.070178719546604 1103 48-49 0.9123396249901745 1103 50-51 0.8264983306380955 1103 52-53 0.839868398135259 1103 54-55 0.9937437285619879 1103 56-57 1.1965147810157717 1103 58-59 1.1726925337830068 1103 60-61 1.285367438304867 1103 62-63 1.2452178180218425 1103 64-65 1.2813170393386528 1103 66-67 1.4839526527022926 1103 68-69 1.4491167498642028 1103 70-71 1.4827762729396916 1103 72-73 1.3357216374467562 1103 74-75 1.3003769494271147 1103 76-77 1.3732323854949904 1103 78-79 1.3444312370243914 1103 80-81 1.4223185995702678 1103 82-83 1.3194087731640636 1103 84-85 1.4926576849952014 1103 86-87 1.3922739453280286 1103 88-89 1.4103343696331585 1103 90-91 1.5365832545034976 1103 92-93 1.7515130955416822 1103 94-95 1.4942715737086267 1103 96-97 1.7898596494728487 1103 98-99 1.9628565048675632 1103 100-101 2.578562846770023 1104 1 0.047597175054669094 1104 2 0.08957910399012547 1104 3 0.27229979511032454 1104 4 0.2566793268338827 1104 5 0.18773905096644228 1104 6 0.18451354853661428 1104 7 0.1910157050267287 1104 8 0.29337851349274047 1104 9 0.36874258824647654 1104 10-11 0.34534666902347766 1104 12-13 0.3364597476723219 1104 14-15 0.5731675659550319 1104 16-17 0.5434628490935225 1104 18-19 0.4005976836238645 1104 20-21 0.5328832497935991 1104 22-23 0.47519356571112326 1104 24-25 0.3930942495626297 1104 26-27 0.4519803367943567 1104 28-29 0.3813069968244136 1104 30-31 0.4285128167143242 1104 32-33 0.40685608216548985 1104 34-35 0.4089215956749044 1104 36-37 0.2547840763010427 1104 38-39 0.3881944283922678 1104 40-41 0.48871160770761435 1104 42-43 0.4141278923631191 1104 44-45 0.42535416774426693 1104 46-47 0.4648395039254041 1104 48-49 0.4147247552978186 1104 50-51 0.385580255267719 1104 52-53 0.29884843667117167 1104 54-55 0.41763769889666236 1104 56-57 0.5364830840904276 1104 58-59 0.39577940729052585 1104 60-61 0.34565863251643947 1104 62-63 0.4136866704865838 1104 64-65 0.42732313066841954 1104 66-67 0.477354076050581 1104 68-69 0.6077155780649477 1104 70-71 0.6789070033227951 1104 72-73 0.5716095618702433 1104 74-75 0.47367763961478104 1104 76-77 0.42965623164480604 1104 78-79 0.5333288408451402 1104 80-81 0.7245200466759663 1104 82-83 0.5238198776564857 1104 84-85 0.5344770570659243 1104 86-87 0.4071845580770237 1104 88-89 0.5880662096270086 1104 90-91 0.764174646285877 1104 92-93 0.7785231732249187 1104 94-95 0.8541343047974657 1104 96-97 0.8809140679141869 1104 98-99 0.9967171449550456 1104 100-101 1.1184399196476242 1105 1 0.05759320024502301 1105 2 0.07705224297182411 1105 3 0.23129093873758322 1105 4 0.09892906909231414 1105 5 0.17640463284131158 1105 6 0.17946119284083295 1105 7 0.14908456860380426 1105 8 0.16905702592023175 1105 9 0.28221905613125386 1105 10-11 0.27401746783601055 1105 12-13 0.23859403413187863 1105 14-15 0.3062140712278705 1105 16-17 0.4448503060848452 1105 18-19 0.32500487802931843 1105 20-21 0.5200505147519294 1105 22-23 0.5586350676923217 1105 24-25 0.4167676568439802 1105 26-27 0.3639134606220935 1105 28-29 0.20967751118961786 1105 30-31 0.2587247858598687 1105 32-33 0.2605753239705493 1105 34-35 0.24016763639185257 1105 36-37 0.18690488567165886 1105 38-39 0.2745661103578101 1105 40-41 0.3051871936815047 1105 42-43 0.23723488927021918 1105 44-45 0.0661198775583216 1105 46-47 0.414213471406498 1105 48-49 0.32089285248191146 1105 50-51 0.12210161365891281 1105 52-53 0.1856471001414306 1105 54-55 0.22422439322138388 1105 56-57 0.2816154949366947 1105 58-59 0.15597727828311037 1105 60-61 0.1583752285888309 1105 62-63 0.3195830521676868 1105 64-65 0.3122601547781301 1105 66-67 0.3272258263331693 1105 68-69 0.2818976367679227 1105 70-71 0.43196108557650703 1105 72-73 0.21206629234088936 1105 74-75 0.28873098901296856 1105 76-77 0.3944170598957655 1105 78-79 0.40776211993888367 1105 80-81 0.5870925558987672 1105 82-83 0.631130422081192 1105 84-85 0.5836234045884972 1105 86-87 0.675796293702497 1105 88-89 0.507801450040887 1105 90-91 0.47917924215952823 1105 92-93 0.5789753078219064 1105 94-95 0.7850223021144664 1105 96-97 0.8138997089143487 1105 98-99 1.1882610479696893 1105 100-101 1.274100297006111 1106 1 -0.09059914120768653 1106 2 0.13076555254482258 1106 3 0.05780688994565608 1106 4 -0.03939450282810952 1106 5 0.07103055885131226 1106 6 0.06832012907383955 1106 7 -0.0057865845293036955 1106 8 -0.001188825479289335 1106 9 0.05908516965119048 1106 10-11 -0.1584379531533031 1106 12-13 -0.24361952704342116 1106 14-15 -0.17514489338133643 1106 16-17 -0.08271930073452438 1106 18-19 -0.014458896913360775 1106 20-21 0.01658294307364372 1106 22-23 -0.10758112679437204 1106 24-25 -0.13922946391755175 1106 26-27 -0.04469105459889278 1106 28-29 0.08384963554548364 1106 30-31 0.2247676963499785 1106 32-33 0.31184260507654216 1106 34-35 -0.12115787267718048 1106 36-37 -0.05941992345110947 1106 38-39 -0.12195889285816008 1106 40-41 -0.08061988220781302 1106 42-43 0.16255653350076926 1106 44-45 -0.03916197378065789 1106 46-47 0.08695335434521212 1106 48-49 0.29498585453256254 1106 50-51 0.19378249680193704 1106 52-53 0.23764483193715336 1106 54-55 0.33799120139070027 1106 56-57 0.24317922220735255 1106 58-59 0.25110305106163366 1106 60-61 0.2316804127314569 1106 62-63 0.4246532643459915 1106 64-65 0.5527607487865893 1106 66-67 0.6326213716480638 1106 68-69 0.71767874843394 1106 70-71 0.5330058972720515 1106 72-73 0.552160728335533 1106 74-75 0.4875410330528318 1106 76-77 0.6455655866034462 1106 78-79 0.46470605991754965 1106 80-81 0.24996579752022186 1106 82-83 0.27393412309441345 1106 84-85 0.25252706171319517 1106 86-87 0.026335772564120674 1106 88-89 0.17933960089633416 1106 90-91 0.4732989840496131 1106 92-93 0.6361568984781911 1106 94-95 0.453883158470358 1106 96-97 0.3674621516890113 1106 98-99 0.6233514356264216 1106 100-101 0.27882115086489634 1107 1 0.4353256301971342 1107 2 0.3982111626574678 1107 3 0.5296606606912029 1107 4 0.41530567202212154 1107 5 0.38184834197969764 1107 6 0.3460274412531561 1107 7 0.378376929100412 1107 8 0.31974733985450854 1107 9 0.4303176487981517 1107 10-11 0.5301485905449255 1107 12-13 0.4251405053468673 1107 14-15 0.69391718561657 1107 16-17 0.6199663343157837 1107 18-19 0.6002641732847138 1107 20-21 0.7582130631756883 1107 22-23 0.7265986894695615 1107 24-25 0.6782329862787932 1107 26-27 0.7233941203946515 1107 28-29 0.5391186110951125 1107 30-31 0.5954206258079466 1107 32-33 0.6914145086509436 1107 34-35 0.5042255079517091 1107 36-37 0.5196335205251614 1107 38-39 0.6783974361469163 1107 40-41 0.8127913454701172 1107 42-43 0.8766343060234831 1107 44-45 0.5839981575003392 1107 46-47 0.8469239379436857 1107 48-49 0.5216981685750426 1107 50-51 0.5989862618146944 1107 52-53 0.5632290395216373 1107 54-55 0.7374415697227406 1107 56-57 0.8925910733266846 1107 58-59 0.98307072526692 1107 60-61 1.0837743159045559 1107 62-63 0.8828137180966706 1107 64-65 0.9283411942247213 1107 66-67 1.043832926095284 1107 68-69 1.140652384745465 1107 70-71 1.0091682213469149 1107 72-73 1.0175919777980802 1107 74-75 0.6717169080205814 1107 76-77 0.9387989185035828 1107 78-79 1.1262221690699512 1107 80-81 1.175960795657062 1107 82-83 1.2227671411240522 1107 84-85 1.0741260814053426 1107 86-87 1.1695651631680697 1107 88-89 1.2035543872064203 1107 90-91 1.3975947460813138 1107 92-93 1.4735667861154198 1107 94-95 1.5254132054792713 1107 96-97 1.4793071799230226 1107 98-99 1.68052172828558 1107 100-101 2.1045836125268345 1108 1 0.08881986864571445 1108 2 0.13256489620392387 1108 3 0.1560441132590178 1108 4 0.13778749917737798 1108 5 0.27024079511746635 1108 6 0.16128621812736554 1108 7 0.15013115324924797 1108 8 0.17931735257804604 1108 9 0.1165155163019449 1108 10-11 0.2273604395573372 1108 12-13 0.2630088841475242 1108 14-15 0.17306928134456712 1108 16-17 0.11363213376812098 1108 18-19 0.22627634784710438 1108 20-21 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-1.4514070439088513 2316 58-59 -1.6996744888975357 2316 60-61 -1.7739313544958222 2316 62-63 -1.5383340993078036 2316 64-65 -1.355853454906459 2316 66-67 -1.3732463090846778 2316 68-69 -1.4072398245415911 2316 70-71 -1.0771130496333008 2316 72-73 -1.3519711115345245 2316 74-75 -1.2242252777319145 2316 76-77 -1.3990088355819275 2316 78-79 -1.2652925018908512 2316 80-81 -1.3290381560966864 2316 82-83 -1.3867280936382436 2316 84-85 -1.1061029338876303 2316 86-87 -1.010027562574006 2316 88-89 -0.8245565588500909 2316 90-91 -0.9039249254627606 2316 92-93 -0.8163838361839169 2316 94-95 -0.9895856891715731 2316 96-97 -0.9135738820605503 2316 98-99 -1.04924283642303 2316 100-101 -1.263962229280164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4184.0 3 644.0 4 575.0 5 690.0 6 755.0 7 956.0 8 1122.0 9 1522.0 10 2039.0 11 2818.0 12 4570.0 13 6805.0 14 8048.0 15 10944.0 16 15144.0 17 21195.0 18 17324.0 19 9476.0 20 8476.0 21 8550.0 22 9266.0 23 9853.0 24 10922.0 25 12345.0 26 14319.0 27 16948.0 28 20403.0 29 24735.0 30 30294.0 31 38314.0 32 49000.0 33 64179.0 34 88152.0 35 125017.0 36 179968.0 37 251108.0 38 203635.0 39 25891.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.35989180757116 19.645597276365155 14.476060573564911 20.518450342498777 2 14.67733974952187 21.596643839842063 41.54350977851811 22.18250663211796 3 20.15374519690844 27.694338694391902 29.235183575836604 22.916732532863055 4 11.993355532968618 16.65065187650669 38.54137908165075 32.81461350887394 5 12.475369847526343 39.70006547475366 32.90033107552116 14.924233602198841 6 29.29582826936875 37.054965177135365 18.28098279318139 15.368223760314494 7 27.28118986906724 35.32718548126708 20.35356699486549 17.038057654800188 8 26.189780796899022 34.12447838601169 19.995222150725734 19.690518666363555 9 27.859329974651896 17.55115284158476 21.1913685432278 33.39814864053554 10-11 24.449450394469032 28.255920503526987 26.370661399372825 20.923967702631156 12-13 26.39094235860729 25.946121321624027 27.591311809759627 20.071624510009052 14-15 23.201577387592444 26.327298677508317 28.058625673701638 22.412498261197598 16-17 20.25767859640255 30.188140589368224 28.15771092084595 21.396469893383273 18-19 20.124933200845938 28.251486148735466 32.139803742447896 19.483776907970704 20-21 21.95890670080514 26.594914749491753 31.824942992322313 19.621235557380796 22-23 20.962283916869037 26.994060037657906 31.797388604996936 20.24626744047612 24-25 20.65377492139313 27.08291353802777 32.28562699214143 19.97768454843767 26-27 20.571493519503136 26.924306871946612 32.58507096953165 19.919128639018602 28-29 19.990559203427974 27.720799038194766 32.49156108388051 19.797080674496748 30-31 20.35508918652625 27.415372889500105 32.24328649391814 19.9862514300555 32-33 20.01890546381777 27.53773867377362 32.1344330306892 20.308922831719407 34-35 20.62445091783419 27.397658790709144 32.07526086219386 19.902629429262806 36-37 20.59861359338057 27.11051763521086 32.2368241094362 20.054044661972366 38-39 20.333342450312784 27.65642136470396 32.1549958415017 19.855240343481558 40-41 20.44909125507801 27.777294127345243 31.902489909468347 19.871124708108404 42-43 20.05547882269315 27.964948171606785 31.399395511994406 20.58017749370566 44-45 20.46695464296282 27.879814330801857 31.048118650754514 20.605112375480815 46-47 20.82693299768413 28.388594861931903 30.246724511445862 20.537747628938106 48-49 20.81508746456795 28.448103123261152 30.008192337274597 20.728617074896306 50-51 20.8333301222795 28.8912385240147 29.829123127987728 20.446308225718074 52-53 20.827015856449453 29.31947766445931 29.328958697383158 20.52454778170808 54-55 20.509659269099508 28.90826487610661 29.48463863753873 21.097437217255152 56-57 21.0040431059189 29.27823471585779 28.940680055346174 20.777042122877138 58-59 21.225763680257202 28.69320622246431 29.268217518911193 20.812812578367296 60-61 20.981944252392985 28.815859260922633 29.775902585164605 20.426293901519777 62-63 20.859379213115258 28.77418087409382 29.874694729705787 20.491745183085136 64-65 20.6744784444239 29.07784043635019 29.694809096792085 20.552872022433824 66-67 20.80366239554103 28.906269560477867 29.2169645797663 21.0731034642148 68-69 20.61153534438394 29.110311950127425 29.36307999519271 20.915072710295927 70-71 20.934508373664674 29.016570133390037 29.155052627199733 20.893868865745553 72-73 20.7614070850388 28.81626093675729 29.32121806027745 21.101113917926458 74-75 20.500600034287675 29.646162766283275 28.889792097269122 20.96344510215993 76-77 20.59455065853593 29.20675216012882 29.045498495710557 21.153198685624695 78-79 20.531117250061733 29.070105643284506 29.110516905891835 21.28826020076192 80-81 20.69079752001649 29.4233595340497 28.796160224346444 21.089682721587373 82-83 20.752471495191042 29.129326560889602 28.955432205465716 21.162769738453648 84-85 20.61336598333017 29.065211538780023 28.809899142167296 21.51152333572251 86-87 20.579784496969587 29.209611669331952 28.816867102356237 21.393736731342226 88-89 20.686900496993122 28.759620262282027 28.84043027286282 21.713048967862036 90-91 20.501313915174467 28.709566581332158 28.982672960311362 21.80644654318201 92-93 20.67457191665399 29.02257753262986 28.969730352269597 21.333120198446554 94-95 20.390489704858222 29.0515357972649 28.93682862795126 21.621145869925616 96-97 20.130289596207795 29.138776678377994 28.915438492825484 21.81549523258873 98-99 20.399897989530828 29.103109161988616 28.604228657545306 21.892764190935246 100-101 20.646755916970026 28.69804126693472 28.993547924886155 21.6616548912091 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1965.0 1 1177.5 2 538.5 3 686.0 4 587.0 5 594.5 6 952.0 7 1789.5 8 2911.5 9 3372.0 10 3083.0 11 2949.5 12 3111.5 13 3295.5 14 3444.5 15 3612.5 16 3768.0 17 3883.0 18 4029.0 19 4249.5 20 4641.0 21 5256.0 22 6048.0 23 6936.5 24 8025.0 25 9488.5 26 11442.0 27 13784.0 28 15517.0 29 17281.5 30 19758.0 31 22454.5 32 25487.0 33 28611.5 34 31942.0 35 34842.5 36 38783.5 37 42430.0 38 42952.5 39 42654.0 40 42468.5 41 42718.0 42 42753.5 43 43736.0 44 45707.0 45 46342.0 46 46414.0 47 49304.0 48 58572.0 49 57078.0 50 45560.0 51 39110.5 52 34797.5 53 32293.5 54 30208.0 55 27204.0 56 23949.5 57 21090.5 58 18791.0 59 16848.5 60 14361.5 61 11890.0 62 9339.0 63 6369.0 64 4143.0 65 2983.0 66 2142.5 67 1606.5 68 1205.5 69 858.0 70 655.0 71 524.0 72 424.5 73 372.0 74 308.0 75 209.5 76 137.5 77 94.0 78 72.0 79 60.0 80 41.0 81 25.0 82 20.5 83 14.0 84 7.0 85 6.0 86 5.5 87 5.0 88 3.5 89 2.0 90 2.0 91 2.5 92 1.5 93 0.5 94 2.0 95 1.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3064160474637227 2 0.26711418997025826 3 0.2593461124617653 4 0.26726801328725813 5 0.2699599213347557 6 0.2019700152208172 7 0.1925867928838257 8 0.19520178927282333 9 0.20458501160981485 10-11 0.2019700152208172 12-13 0.19939347466106952 14-15 0.20031641456306873 16-17 0.185626287789582 18-19 0.1891642240805788 20-21 0.18008864837758704 22-23 0.18901040076357895 24-25 0.18747216759358035 26-27 0.18866429830032924 28-29 0.20250839683031674 30-31 0.2002395029045688 32-33 0.20646934724306312 34-35 0.19789369732032092 36-37 0.1828574680835845 38-39 0.1729358641370935 40-41 0.16874417874884728 42-43 0.1846264362290829 44-45 0.18466489205833286 46-47 0.18531864115558228 48-49 0.20296986678131632 50-51 0.19935501883181958 52-53 0.22042881326080052 54-55 0.18643386020383126 56-57 0.1677443271883482 58-59 0.1706285143820956 60-61 0.1595532355581056 62-63 0.16635991733534944 64-65 0.16559080075035015 66-67 0.16424484672660136 68-69 0.1665137406523493 70-71 0.16882109040734722 72-73 0.16628300567684953 74-75 0.1807808533040864 76-77 0.17212829172284422 78-79 0.17593541881859076 80-81 0.16924410452909683 82-83 0.15313111207336141 84-85 0.1522466280006122 86-87 0.15543846182835933 88-89 0.1604761754601048 90-91 0.16643682899384937 92-93 0.1623220552641031 94-95 0.16189904114235348 96-97 0.16624454984759957 98-99 0.1758969629893408 100-101 0.16024544048460498 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1300193.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.27609744321259 #Duplication Level Percentage of deduplicated Percentage of total 1 78.29215170971136 44.84268910364211 2 12.792002680320627 14.653519840237617 3 4.030178566643254 6.924987008898177 4 1.683149623922988 3.856169674852788 5 0.8737867592196951 2.5023547782828093 6 0.5011199442518444 1.7221316854623543 7 0.34537745944435855 1.3847311115277003 8 0.2328019590945858 1.066719015525783 9 0.1631293714970026 0.8409072399941115 >10 0.9648416505223533 10.216655084414526 >50 0.0793187929868036 3.143334288770279 >100 0.03650474515628815 3.666590361423805 >500 0.0033552007314193574 1.3279140758771049 >1k 0.0018789124095948402 1.9783037138508348 >5k 2.684160585135486E-4 0.991457930573829 >10k+ 1.342080292567743E-4 0.881535086666202 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11468 0.8820228996772018 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7507 0.5773758203589775 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5391 0.4146307509731248 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4840 0.3722524271396631 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2258 0.17366652489284284 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2201 0.1692825603583468 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1935 0.1488240591973653 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1858 0.14290186149287068 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1809 0.1391331902263741 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1672 0.12859629301188363 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1590 0.12228953701488932 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1473 0.11329087297039747 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1423 0.10944529004540095 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.84558292499652E-5 0.0 20-21 0.0 0.0 0.0 7.69116584999304E-5 0.0 22-23 0.0 0.0 0.0 1.153674877498956E-4 0.0 24-25 0.0 0.0 0.0 1.92279146249826E-4 0.0 26-27 0.0 0.0 0.0 6.152932679994431E-4 0.0 28-29 0.0 0.0 0.0 0.0023073497549979116 0.0 30-31 0.0 0.0 0.0 0.006191388509244397 0.0 32-33 0.0 0.0 0.0 0.010036971434240916 0.0 34-35 0.0 0.0 0.0 0.014459391797986915 0.0 36-37 0.0 0.0 0.0 0.020304677843981624 0.0 38-39 0.0 0.0 0.0 0.028880327766723862 0.0 40-41 0.0 0.0 0.0 0.04091700232196297 0.0 42-43 0.0 0.0 0.0 0.05749146472869797 0.0 44-45 0.0 0.0 0.0 0.0704895350151862 0.0 46-47 0.0 0.0 0.0 0.09821618790441111 0.0 48-49 0.0 0.0 0.0 0.13378782996062893 0.0 50-51 0.0 0.0 0.0 0.15228508382986217 0.0 52-53 0.0 0.0 0.0 0.18435724542433315 0.0 54-55 0.0 0.0 0.0 0.23138872459704057 0.0 56-57 0.0 0.0 0.0 0.3122613335097174 0.0 58-59 0.0 0.0 0.0 0.41555369087512395 0.0 60-61 0.0 0.0 0.0 0.5358435247690151 0.0 62-63 0.0 0.0 0.0 0.6670163583406463 0.0 64-65 0.0 0.0 0.0 0.812225569588515 0.0 66-67 0.0 0.0 0.0 0.9926603204293516 0.0 68-69 0.0 0.0 0.0 1.2203572854183955 0.0 70-71 0.0 0.0 0.0 1.37018119617626 0.0 72-73 0.0 0.0 0.0 1.5384254491448577 0.0 74-75 0.0 0.0 0.0 1.7426259024621729 0.0 76-77 0.0 0.0 0.0 1.9682077968424687 0.0 78-79 0.0 0.0 0.0 2.2027883552672565 0.0 80-81 0.0 0.0 0.0 2.5271632749907127 0.0 82-83 0.0 0.0 0.0 2.8746116922641485 0.0 84-85 0.0 0.0 0.0 3.3065091105705076 0.0 86-87 0.0 0.0 0.0 3.874963178543493 0.0 88-89 0.0 0.0 0.0 4.48606476115469 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCG 65 1.0034069E-5 25.574736 76-77 GTATCAA 17575 0.0 24.887676 1 GTACATG 11985 0.0 24.753603 1 TACATGG 11805 0.0 24.557728 2 GAGTACT 8350 0.0 23.778242 12-13 ACATGGG 11875 0.0 23.372414 3 CGCGAAT 85 3.155972E-6 22.349304 78-79 AGTACTT 8750 0.0 22.094097 12-13 GTACTTT 9045 0.0 21.688175 14-15 TATCAAC 20195 0.0 21.579918 2 CATGGGA 8790 0.0 21.577883 4 ATCAACG 20195 0.0 21.556385 3 TCAACGC 20215 0.0 21.540041 4 CAACGCA 20365 0.0 21.357222 5 AACGCAG 20830 0.0 20.868378 6 TATTCCG 115 0.003707943 20.667135 5 CGGGTCG 230 9.008909E-8 20.655184 6 ATGGGAG 2925 0.0 20.638872 5 ATGGGAT 3210 0.0 20.435352 5 GGTATCA 13080 0.0 19.991564 1 >>END_MODULE