FastQCFastQC Report
Thu 26 May 2016
SRR937626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937626_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300193
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157941.2147427343479007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107400.8260312122892525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83450.6418277901819192No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46740.35948509182867466No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29290.22527424774629615No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24050.1849725386923326No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22510.17312814328334333No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21470.16512933079935055No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20940.16105301289885424No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20320.15628449007185855No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16610.1277502647683844No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13720.1055227954619045No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13350.10267706409740707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG110450.025.0741862
GTACATG110500.025.0676611
GTATCAA196800.024.6977881
ACATGGG110800.023.9660283
ATGGGAG25550.023.2403765
AGTACTT123350.022.60595912-13
CATGGGG27150.022.2207094
GAGTACT120200.022.21037312-13
CATGGGA81850.021.3576354
TATCAAC232600.020.8924772
GTACTTT127300.020.8564614-15
ATCAACG234750.020.701133
TCAACGC235700.020.6176934
GGACCGA1856.5579425E-620.538826
CATGGGT8350.020.4804764
CAACGCA237700.020.4642015
AACGCAG242700.020.1178026
TACTTTT138500.020.02720314-15
TACCTGG9800.019.8738382
GGTATCA146900.019.7618221
Produced by FastQC (version 0.11.5)