##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937626_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1300193 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.221366366377914 33.0 31.0 34.0 31.0 34.0 2 32.41033292749615 34.0 31.0 34.0 31.0 34.0 3 32.49172930480321 34.0 31.0 34.0 31.0 34.0 4 36.122281076732456 37.0 35.0 37.0 35.0 37.0 5 36.05128469388775 37.0 35.0 37.0 35.0 37.0 6 36.10950912672195 37.0 35.0 37.0 35.0 37.0 7 36.057580682252556 37.0 35.0 37.0 35.0 37.0 8 36.05222839993755 37.0 35.0 37.0 35.0 37.0 9 37.70970540527445 39.0 37.0 39.0 35.0 39.0 10-11 37.650585336177016 39.0 37.0 39.0 35.0 39.0 12-13 37.55154926999299 39.0 37.0 39.0 35.0 39.0 14-15 38.684569906160085 40.0 38.0 41.0 34.5 41.0 16-17 38.68734257144901 40.0 38.0 41.0 34.5 41.0 18-19 38.77205114932937 40.0 38.0 41.0 35.0 41.0 20-21 38.71741579903907 40.0 38.0 41.0 34.5 41.0 22-23 38.563200232580854 40.0 38.0 41.0 34.0 41.0 24-25 38.613033603472715 40.0 38.0 41.0 34.0 41.0 26-27 38.4550705164541 40.0 38.0 41.0 34.0 41.0 28-29 38.30024850156862 40.0 38.0 41.0 34.0 41.0 30-31 38.2400301339878 40.0 37.5 41.0 34.0 41.0 32-33 38.053068275248364 40.0 37.0 41.0 34.0 41.0 34-35 37.86338143644828 40.0 37.0 41.0 33.0 41.0 36-37 37.64804609777164 40.0 36.0 41.0 33.0 41.0 38-39 37.45288891726075 40.0 36.0 41.0 33.0 41.0 40-41 37.210719100933474 40.0 36.0 41.0 33.0 41.0 42-43 36.88108919214301 40.0 36.0 41.0 31.5 41.0 44-45 36.37853841698886 40.0 35.0 41.0 30.5 41.0 46-47 35.74984944542849 39.0 35.0 41.0 28.5 41.0 48-49 35.19839516133374 39.0 35.0 41.0 25.0 41.0 50-51 34.86436052186099 39.0 35.0 41.0 23.5 41.0 52-53 34.600442011301396 39.0 35.0 40.0 22.5 41.0 54-55 34.39313086595605 39.0 35.0 40.0 21.0 41.0 56-57 34.13735883826478 38.0 34.0 40.0 19.5 41.0 58-59 33.89197411461222 38.0 34.0 40.0 18.0 41.0 60-61 33.54435726080666 38.0 34.0 40.0 15.0 41.0 62-63 33.21620559409257 37.5 33.0 40.0 11.5 41.0 64-65 32.848735918436724 37.0 33.0 39.5 8.0 41.0 66-67 32.428929781963134 36.0 32.0 39.0 7.0 40.5 68-69 31.99746306894438 36.0 32.0 39.0 2.0 40.0 70-71 31.57880406985732 35.0 31.0 38.5 2.0 40.0 72-73 31.099524839773785 35.0 31.0 37.5 2.0 40.0 74-75 30.61140807557032 35.0 30.5 37.0 2.0 39.0 76-77 29.360855273024853 33.5 29.0 35.5 2.0 38.5 78-79 29.938292622710627 34.0 30.0 36.0 2.0 38.5 80-81 29.73293464893289 34.0 30.0 36.0 2.0 37.5 82-83 29.37915640216491 34.0 30.0 35.0 2.0 37.0 84-85 28.995270702118837 34.0 29.0 35.0 2.0 36.5 86-87 28.5926843168668 34.0 29.0 35.0 2.0 36.0 88-89 28.218871352176176 34.0 29.0 35.0 2.0 36.0 90-91 27.71852101957171 34.0 27.5 35.0 2.0 35.0 92-93 27.292818835357522 33.0 26.5 35.0 2.0 35.0 94-95 26.850321836834993 33.0 26.0 35.0 2.0 35.0 96-97 26.326399234575174 33.0 25.0 35.0 2.0 35.0 98-99 25.512600437012043 32.5 22.0 34.5 2.0 35.0 100-101 22.98890010944529 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22601944573913357 1101 2 0.21316529618612634 1101 3 0.15765833025831455 1101 4 0.10725516761807796 1101 5 0.02445218100292834 1101 6 0.05204320867999712 1101 7 0.10409292833048056 1101 8 0.09354725777210149 1101 9 0.23432715814342941 1101 10-11 0.2286867287900023 1101 12-13 0.17883559201928279 1101 14-15 0.30440130714418245 1101 16-17 0.2488005935012083 1101 18-19 0.21945377996535598 1101 20-21 0.28741575801507935 1101 22-23 0.1707248575497644 1101 24-25 0.3022583241641428 1101 26-27 0.22883760920729657 1101 28-29 0.2477219265607289 1101 30-31 0.23991012550506952 1101 32-33 0.17645037722542867 1101 34-35 0.028089487056867313 1101 36-37 0.05075611731184182 1101 38-39 0.1298982350285698 1101 40-41 -0.04124319084197481 1101 42-43 0.058377049830760086 1101 44-45 0.19952339305421418 1101 46-47 0.14313981518666452 1101 48-49 -0.008586695654997811 1101 50-51 0.09152803825283229 1101 52-53 0.08062667542255753 1101 54-55 0.10552596937537118 1101 56-57 0.1389122452772682 1101 58-59 0.19049449667883067 1101 60-61 0.1686945963068993 1101 62-63 0.29806996636177985 1101 64-65 0.3027224183096635 1101 66-67 0.037101555288742816 1101 68-69 0.16164446832332757 1101 70-71 0.19123971163404363 1101 72-73 0.27637519693304213 1101 74-75 0.23544837887467907 1101 76-77 0.15636037410388326 1101 78-79 0.15003560205239097 1101 80-81 0.22643615227183744 1101 82-83 0.3381145610319649 1101 84-85 0.1953021533410677 1101 86-87 0.041211023732504515 1101 88-89 0.24633345379330152 1101 90-91 0.20003701313996913 1101 92-93 0.4104247218943158 1101 94-95 0.48344691501772274 1101 96-97 0.4278422257553274 1101 98-99 0.38902932929487477 1101 100-101 0.7019580157818588 1102 1 0.24783633888065282 1102 2 0.217503757043076 1102 3 0.096460283811588 1102 4 0.05601315514811489 1102 5 0.07552530635592802 1102 6 0.1412903597481403 1102 7 0.09046013875828862 1102 8 0.1346145136056336 1102 9 0.20477468131696241 1102 10-11 0.14965586397721609 1102 12-13 0.20136541028567478 1102 14-15 0.267081407258253 1102 16-17 0.2746070987007201 1102 18-19 0.16338602577640415 1102 20-21 0.1714442971692094 1102 22-23 0.1917789900237139 1102 24-25 0.030727985393198765 1102 26-27 0.08996117920408864 1102 28-29 0.21565413914807863 1102 30-31 0.234392223470671 1102 32-33 0.24224713680769838 1102 34-35 0.25336552059064843 1102 36-37 0.3030125599400506 1102 38-39 0.2882368287803061 1102 40-41 0.2628352946005137 1102 42-43 0.18695136569950677 1102 44-45 0.20715930565374663 1102 46-47 0.2740287151501164 1102 48-49 0.4240605683737044 1102 50-51 0.5761025534637483 1102 52-53 0.5863453039484199 1102 54-55 0.5419787308181654 1102 56-57 0.49767277995727 1102 58-59 0.7236600503517039 1102 60-61 0.5708452768389236 1102 62-63 0.5169241364892443 1102 64-65 0.5847431388208619 1102 66-67 0.6296779974739692 1102 68-69 0.4082971810380336 1102 70-71 0.4000603258294255 1102 72-73 0.5040765602120025 1102 74-75 0.38158141762458087 1102 76-77 0.36525158865143226 1102 78-79 0.44894088129754905 1102 80-81 0.5019657933451853 1102 82-83 0.4063851015814848 1102 84-85 0.6187755245494166 1102 86-87 0.8418120957503987 1102 88-89 0.8036291003817837 1102 90-91 0.7134109347541973 1102 92-93 0.7397809020553368 1102 94-95 0.7836576086177338 1102 96-97 0.8449158826874523 1102 98-99 0.7333209447416813 1102 100-101 0.7249571187420898 1103 1 0.25860889056185243 1103 2 0.26743333486723486 1103 3 0.18938183452831225 1103 4 0.1336918769345914 1103 5 0.19481732534610074 1103 6 0.10480223200843852 1103 7 0.1705032765935428 1103 8 0.12471245826468902 1103 9 0.2339675649349573 1103 10-11 0.16263506221904578 1103 12-13 0.17994171713584706 1103 14-15 0.25216070875191576 1103 16-17 0.24442804946813368 1103 18-19 0.12211590910353465 1103 20-21 0.17962598468185575 1103 22-23 0.2792825834258039 1103 24-25 0.23432483204466337 1103 26-27 0.22881937887986936 1103 28-29 0.15569386644074967 1103 30-31 0.2910739951837513 1103 32-33 0.41533557447301206 1103 34-35 0.5497705875852716 1103 36-37 0.3639294430613589 1103 38-39 0.4028816645724618 1103 40-41 0.41420576287395505 1103 42-43 0.5044943546956162 1103 44-45 0.5393699666185938 1103 46-47 0.5615470223643015 1103 48-49 0.7073244150915059 1103 50-51 0.7712325793362425 1103 52-53 0.765119184125254 1103 54-55 0.6957603931557443 1103 56-57 0.6257114373091568 1103 58-59 0.8080739558397099 1103 60-61 0.6571064886786928 1103 62-63 0.7912846384938987 1103 64-65 0.8745505920045744 1103 66-67 0.6779556734741305 1103 68-69 0.7851544808749047 1103 70-71 0.8365725655097656 1103 72-73 0.7115221743903319 1103 74-75 0.8107061329165717 1103 76-77 0.7912445158155101 1103 78-79 0.9049618922810261 1103 80-81 0.8768737257058774 1103 82-83 0.9316067443233216 1103 84-85 0.8900061520722389 1103 86-87 0.9358493021333025 1103 88-89 0.9931091925089568 1103 90-91 0.9175100071886781 1103 92-93 1.0440776328674204 1103 94-95 1.2058209047157682 1103 96-97 1.3270330726725312 1103 98-99 1.2449933353364067 1103 100-101 1.3662777556715469 1104 1 0.26174320901245096 1104 2 0.32334665875583823 1104 3 0.1445480228442051 1104 4 0.05777472740441425 1104 5 0.10319932351887928 1104 6 0.06585580321865336 1104 7 0.12027775589577061 1104 8 0.08468384456190137 1104 9 0.1506611624879426 1104 10-11 0.24603208345921956 1104 12-13 0.23377321072571533 1104 14-15 0.43344917260834137 1104 16-17 0.322775311164726 1104 18-19 0.15035905744932876 1104 20-21 0.1717644222479109 1104 22-23 0.15366288189571975 1104 24-25 0.138846251270337 1104 26-27 0.07484101733752624 1104 28-29 -0.041731730071099093 1104 30-31 0.2940141928943092 1104 32-33 0.1063761489830668 1104 34-35 0.030936071913245655 1104 36-37 0.08960321016538586 1104 38-39 0.04739134484315599 1104 40-41 0.030494349011156885 1104 42-43 0.20114652526682875 1104 44-45 0.31891125647121754 1104 46-47 0.3391219935846479 1104 48-49 0.33537619401484164 1104 50-51 0.30285722799735737 1104 52-53 0.19583161815913996 1104 54-55 0.26979813075166703 1104 56-57 0.33892522953314597 1104 58-59 0.23176310586599413 1104 60-61 0.24223334625477122 1104 62-63 0.18512068267569504 1104 64-65 0.15638748516436607 1104 66-67 0.26914110703810934 1104 68-69 0.2857573687975865 1104 70-71 0.31630605454729377 1104 72-73 0.24118312472162629 1104 74-75 -0.08637777615544096 1104 76-77 0.17298022162891513 1104 78-79 0.3413505565032402 1104 80-81 0.347553196334907 1104 82-83 0.47181353859289743 1104 84-85 0.34221663922140166 1104 86-87 0.2885961651945479 1104 88-89 0.47561408810737404 1104 90-91 0.2615207818723633 1104 92-93 0.2009564508664532 1104 94-95 0.2384235812673765 1104 96-97 0.2121391425158201 1104 98-99 0.39363021032876233 1104 100-101 0.7490098314926179 1105 1 0.12354715152677187 1105 2 0.09234500672557999 1105 3 0.0752590169192544 1105 4 0.08289055584169347 1105 5 0.02385218600288397 1105 6 0.03801971443134278 1105 7 0.09550040734222875 1105 8 0.1312325918758006 1105 9 0.1636041363908376 1105 10-11 0.11176270315687731 1105 12-13 0.2255254807146585 1105 14-15 0.11541005799718818 1105 16-17 0.2035884702689046 1105 18-19 0.24898246907813615 1105 20-21 0.22431420052806317 1105 22-23 0.1866305120396774 1105 24-25 0.09894288882981073 1105 26-27 0.06935236417692892 1105 28-29 0.13698720120195418 1105 30-31 0.08149385304808021 1105 32-33 0.14632609122411822 1105 34-35 0.10247798752305926 1105 36-37 0.07653136548106687 1105 38-39 0.18352857514611287 1105 40-41 0.14768602178495627 1105 42-43 0.12902391481059539 1105 44-45 0.0752336880610116 1105 46-47 -0.21728148685804882 1105 48-49 -0.17362768142455565 1105 50-51 -0.04655182960011928 1105 52-53 0.06523583145736467 1105 54-55 0.2336413369325001 1105 56-57 -0.0374721644822813 1105 58-59 -0.09887739744427648 1105 60-61 -0.03178475155038285 1105 62-63 -0.004339550595950925 1105 64-65 -0.0296972652300056 1105 66-67 -0.17718809414937198 1105 68-69 -0.13707387569047214 1105 70-71 -0.16052981065697125 1105 72-73 -0.07419317796012947 1105 74-75 -0.10125691709970397 1105 76-77 -0.1839856322719875 1105 78-79 -0.007910998392606672 1105 80-81 -0.1387228417735784 1105 82-83 -0.0899450662489194 1105 84-85 0.003739860804618189 1105 86-87 0.12411357361866138 1105 88-89 0.14787084838908626 1105 90-91 0.03952145262148932 1105 92-93 0.19920194504708633 1105 94-95 0.11639071456013284 1105 96-97 0.10178383179749773 1105 98-99 0.19648741529230662 1105 100-101 0.48517718339990523 1106 1 0.03965007768054818 1106 2 0.12349072418943052 1106 3 0.09487643006916358 1106 4 0.07384847961186836 1106 5 -0.02546233958487676 1106 6 0.09522849894626262 1106 7 0.07416907169339737 1106 8 0.007300955817598265 1106 9 -0.05758503449442287 1106 10-11 0.016770534988168606 1106 12-13 -0.07043237684438708 1106 14-15 0.10956432426431206 1106 16-17 0.2872072208837224 1106 18-19 0.11299402197515462 1106 20-21 0.18492685215155547 1106 22-23 0.10064234869870603 1106 24-25 0.15704032135120372 1106 26-27 0.0721117131890594 1106 28-29 0.23945436993819413 1106 30-31 0.3323309509573207 1106 32-33 0.13356365866488318 1106 34-35 0.028486572269528665 1106 36-37 0.06460863198277167 1106 38-39 0.08028531733130961 1106 40-41 0.22233954837913217 1106 42-43 0.08868738253361386 1106 44-45 0.06044353952106718 1106 46-47 -0.05170777684011085 1106 48-49 0.1909031559381944 1106 50-51 0.2582598753933354 1106 52-53 0.24150074787999642 1106 54-55 0.3079106024807672 1106 56-57 0.17441954437033758 1106 58-59 0.19652807008200313 1106 60-61 0.25458928082118604 1106 62-63 0.22201905458138782 1106 64-65 0.06747064809772496 1106 66-67 0.01227766712733569 1106 68-69 0.13692364044612404 1106 70-71 0.2907489628101452 1106 72-73 0.2816327870198556 1106 74-75 0.23223532295538263 1106 76-77 0.23178079943973628 1106 78-79 0.2303407817410239 1106 80-81 0.30689440679436686 1106 82-83 0.14874583558456322 1106 84-85 0.11043200929916352 1106 86-87 0.31375242971727246 1106 88-89 0.0654429523140827 1106 90-91 0.27530284804951677 1106 92-93 0.39374562736984586 1106 94-95 0.6181618623963523 1106 96-97 0.6193524832847892 1106 98-99 0.8211201799665879 1106 100-101 1.068305498685973 1107 1 0.3422198027930534 1107 2 0.3148800669245446 1107 3 0.2497908681119938 1107 4 0.16331052851260353 1107 5 0.2672523535673008 1107 6 0.19172038704211047 1107 7 0.22689071585884335 1107 8 0.19895006580125596 1107 9 0.4287619572216883 1107 10-11 0.41230468811111365 1107 12-13 0.3397571278421765 1107 14-15 0.41632525956972444 1107 16-17 0.4323606806251732 1107 18-19 0.4798848050748816 1107 20-21 0.4625186126927332 1107 22-23 0.5161526032347794 1107 24-25 0.4752032473694925 1107 26-27 0.4407778710702175 1107 28-29 0.4956341432061748 1107 30-31 0.3817583881115638 1107 32-33 0.3616975265813025 1107 34-35 0.4008118653586479 1107 36-37 0.43002769925711704 1107 38-39 0.4955286386257711 1107 40-41 0.4320505438788871 1107 42-43 0.43718126994152584 1107 44-45 0.17327747479780697 1107 46-47 0.05185694640923799 1107 48-49 0.08338011727049377 1107 50-51 0.08389066193272754 1107 52-53 0.018937543821174074 1107 54-55 -0.04946015883434285 1107 56-57 -0.056049541535585945 1107 58-59 0.04594816209334596 1107 60-61 0.11343892395472466 1107 62-63 0.21889378585664332 1107 64-65 0.2529847722700467 1107 66-67 0.2844411879230435 1107 68-69 0.2761472409276813 1107 70-71 0.3514834412691705 1107 72-73 0.6370364832390791 1107 74-75 0.5385241984677513 1107 76-77 0.38602969587680747 1107 78-79 0.5013066859460302 1107 80-81 0.507761671104852 1107 82-83 0.41343522561523116 1107 84-85 0.5425037695537007 1107 86-87 0.6602523795553239 1107 88-89 0.6014565462029253 1107 90-91 0.7400381004357932 1107 92-93 0.908150169176249 1107 94-95 1.0970956342884612 1107 96-97 1.2283830677431844 1107 98-99 1.5233471914608394 1107 100-101 1.73219623878974 1108 1 0.06932837119420299 1108 2 0.10288062548967503 1108 3 0.0601160525074107 1108 4 0.0031317876694458846 1108 5 -0.022575827721851738 1108 6 -0.018036403389885436 1108 7 0.013300082972186544 1108 8 0.04423411594985538 1108 9 0.04201852980128962 1108 10-11 -0.01150930490442903 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-0.545933825188758 2316 82-83 -0.5877052010413877 2316 84-85 -0.44467540387354276 2316 86-87 -0.5390412574675914 2316 88-89 -0.7790747882023119 2316 90-91 -0.7085227839087942 2316 92-93 -0.547346679151083 2316 94-95 -0.4257826459677183 2316 96-97 -0.47802436803440074 2316 98-99 -0.4894104140347437 2316 100-101 0.20356565626665457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 7.0 5 22.0 6 53.0 7 134.0 8 374.0 9 729.0 10 1390.0 11 2497.0 12 4101.0 13 6428.0 14 8348.0 15 12477.0 16 18037.0 17 26242.0 18 22287.0 19 10678.0 20 7562.0 21 7192.0 22 7175.0 23 7608.0 24 8416.0 25 9593.0 26 11364.0 27 14127.0 28 17387.0 29 22175.0 30 27169.0 31 34295.0 32 44553.0 33 58844.0 34 82408.0 35 119775.0 36 181964.0 37 277302.0 38 226810.0 39 20662.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4884897205608 19.886531915302804 15.465738407985077 20.159239956151318 2 14.977776376276445 21.99965697400309 40.866394450670015 22.15617219905045 3 20.127242647822285 27.39424070118821 30.101531080385758 22.37698557060375 4 12.217445142633 17.119959390550612 38.23471954099016 32.42787592582623 5 12.221984602224122 39.48010966761236 33.11067997612175 15.18722575404176 6 29.22589184836405 37.54727182810552 17.672376331821507 15.554459991708924 7 26.637814539841393 35.61871199121976 20.37820538950756 17.365268079431285 8 26.457533612317558 33.6953052354535 19.828287031233057 20.018874120995882 9 27.610670108207014 17.11476680769701 21.209235859599307 34.06532722449667 10-11 24.44517852349613 28.216926256332712 26.222107025649272 21.11578819452189 12-13 26.615334088631382 25.834577421510918 27.802621084198808 19.747467405658888 14-15 23.18394269158502 26.005562251142717 28.820951966361918 21.98954309091035 16-17 19.786177898204343 29.70939698952386 29.078029184897936 21.42639592737386 18-19 19.575363042256033 27.164851679712164 33.97018750293226 19.289597775099544 20-21 21.099521378749156 25.5223647566169 34.061327818254675 19.31678604637927 22-23 20.347090135911845 25.830672806324657 34.01057237415406 19.811664683609436 24-25 20.173197363775994 25.932611543055533 34.505069631970024 19.389121461198453 26-27 20.048631409984015 25.77303214110877 34.82375824736182 19.35457820154539 28-29 19.459835499447 26.552364368571073 34.71493903142493 19.272861100557 30-31 19.787716131374342 26.194534196077047 34.45088536855682 19.566864303991792 32-33 19.51560268360159 26.304402500244194 34.39489368116887 19.785101134985343 34-35 20.072366179482586 26.10977754841012 34.38424141646663 19.433614855640663 36-37 20.169351780850995 25.840932846123614 34.34651624797242 19.643199125052973 38-39 19.8872548963055 26.416610809424316 34.279080087941026 19.417054206329155 40-41 19.97040413203849 26.49045537103858 34.1246202933975 19.41452020352543 42-43 19.72298975067462 26.70690037138804 33.430731668292324 20.139378209645013 44-45 20.08878681857232 26.622932133921655 32.97187417560316 20.316406871902863 46-47 20.536143480237165 27.121819606781454 32.10446449104094 20.237572421940435 48-49 20.53199025067817 27.458923405986653 31.510245017470485 20.498841325864696 50-51 20.645973328575064 27.797565438361843 31.265435208465203 20.291026024597887 52-53 20.626245488169832 28.40528290799904 30.469284175503176 20.499187428327946 54-55 20.390165152404297 28.03964488349037 30.661447954265252 20.90874200984008 56-57 20.854250099792875 28.28241653354541 30.103684606823755 20.75964875983796 58-59 21.04779828840795 27.804410575968337 30.36733777216152 20.780453363462193 60-61 20.769647275442953 27.89939647421575 30.98366934755071 20.347286902790586 62-63 20.864979276153615 27.82633039864082 30.92667780860226 20.382012516603304 64-65 20.615939325930842 28.04618237446287 30.925062663773762 20.412815635832526 66-67 20.713886323030504 28.067409992208848 30.280927523836848 20.9377761609238 68-69 20.692652079579076 28.115984497662268 30.40272544572297 20.78863797703568 70-71 20.89509019045634 28.081715560689837 30.215629525770403 20.80756472308342 72-73 20.742651283309478 27.831022009809313 30.38106650320376 21.045260203677454 74-75 20.52079960436643 28.724081732481256 29.719664695933606 21.03545396721871 76-77 20.460462408273234 28.190276366662488 30.21728312642815 21.131978098636125 78-79 20.42858252582501 28.171894480281008 30.11522135559875 21.28430163829524 80-81 20.47396827777425 28.44805673006113 29.835543582969443 21.242431409195177 82-83 20.559008122077284 28.208028242677823 30.026496738862907 21.206466896381983 84-85 20.391312490458112 28.07562134259763 29.833481837441262 21.69958432950299 86-87 20.306677547102623 28.172009847768752 29.70243648442962 21.818876120699006 88-89 20.32878964892135 27.818408497815323 29.897292171239194 21.95550968202413 90-91 20.108881683954664 27.679793236335943 30.11165084016969 22.099674239539706 92-93 20.08933498697278 27.86374780075567 30.166613788661035 21.88030342361051 94-95 19.828440854550056 27.874707831837277 30.170982308011197 22.125869005601476 96-97 19.619702613381243 27.87889951722552 30.25412381084962 22.247274058543617 98-99 19.75810902014925 27.864879467916758 29.81938935025929 22.5576221616747 100-101 19.46503828820253 27.61085385634068 30.331125929703866 22.592981925752927 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 778.0 1 568.5 2 587.5 3 755.5 4 584.5 5 560.0 6 1059.0 7 2278.0 8 3580.0 9 4021.5 10 3652.5 11 3346.0 12 3530.0 13 3838.5 14 4056.5 15 4266.5 16 4443.0 17 4642.5 18 4929.0 19 5204.5 20 5512.0 21 6111.0 22 6860.0 23 7705.0 24 8687.5 25 10017.5 26 11832.0 27 14114.5 28 15823.0 29 17574.5 30 20107.0 31 22414.0 32 25133.0 33 28156.5 34 31543.5 35 34561.5 36 38278.0 37 41732.5 38 42537.5 39 42118.5 40 41849.5 41 42116.0 42 42250.5 43 43582.0 44 45565.0 45 45983.5 46 45630.0 47 48720.0 48 58957.5 49 56943.5 50 44566.0 51 38588.0 52 34478.0 53 32137.0 54 30121.0 55 26987.5 56 23581.5 57 20746.0 58 18444.0 59 16368.0 60 13687.5 61 11216.0 62 9017.0 63 6170.0 64 3931.5 65 2856.0 66 2031.5 67 1501.5 68 1158.5 69 841.0 70 637.5 71 499.0 72 412.5 73 403.5 74 323.5 75 235.0 76 180.0 77 116.5 78 85.5 79 59.5 80 38.0 81 23.5 82 17.0 83 12.5 84 7.5 85 3.5 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020612324477981343 2 0.0 3 0.0 4 2.3073497549979118E-4 5 0.020381589502481555 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0033841129739969377 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.229399019991647E-4 24-25 0.0 26-27 3.461024632496868E-4 28-29 0.001153674877498956 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.6919080474975635E-4 40-41 8.844840727491995E-4 42-43 0.01576688999248573 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.5382331699986078E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.8455829249965194E-5 82-83 0.002538084730497703 84-85 3.461024632496868E-4 86-87 0.0 88-89 0.0 90-91 0.012152042042989003 92-93 0.002345805584247877 94-95 0.0 96-97 0.0 98-99 0.0015766889992485733 100-101 0.019189458795732636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1300193.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.085972844787634 #Duplication Level Percentage of deduplicated Percentage of total 1 77.37607102853961 42.623361475144925 2 13.24994003645481 14.597716740864284 3 4.172607116527912 6.895563669390725 4 1.7852857079753455 3.9337680011886937 5 0.9471068168493879 2.608615019703932 6 0.5607704700586744 1.8534352131486562 7 0.34803906074495655 1.3420449174385447 8 0.23930023580756193 1.0545669033157308 9 0.17237123851655975 0.854571362773106 >10 1.014121023107581 10.36318452384634 >50 0.08568091372255159 3.2528349070396123 >100 0.04270530537601035 4.3059995140622185 >500 0.0032098619846114415 1.2677877137591236 >1k 0.0023725066842780218 2.365137894233184 >5k 1.3955921672223658E-4 0.6415431733260497 >10k+ 2.7911843344447315E-4 2.0398689707649376 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15794 1.2147427343479007 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10740 0.8260312122892525 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8345 0.6418277901819192 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4674 0.35948509182867466 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2929 0.22527424774629615 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2405 0.1849725386923326 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2251 0.17312814328334333 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2147 0.16512933079935055 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2094 0.16105301289885424 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2032 0.15628449007185855 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1661 0.1277502647683844 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1372 0.1055227954619045 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1335 0.10267706409740707 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.84558292499652E-5 0.0 20-21 0.0 0.0 0.0 1.153674877498956E-4 0.0 22-23 0.0 0.0 0.0 1.92279146249826E-4 0.0 24-25 0.0 0.0 0.0 3.0764663399972155E-4 0.0 26-27 0.0 0.0 0.0 7.69116584999304E-4 0.0 28-29 0.0 0.0 0.0 0.0024996289012477377 0.0 30-31 0.0 0.0 0.0 0.006460579313994153 0.0 32-33 0.0 0.0 0.0 0.010344618068240639 0.0 34-35 0.0 0.0 0.0 0.014882405919736531 0.0 36-37 0.0 0.0 0.0 0.021035338599730963 0.0 38-39 0.0 0.0 0.0 0.029841723497972993 0.0 40-41 0.0 0.0 0.0 0.04195530971171203 0.0 42-43 0.0 0.0 0.0 0.058606683776946965 0.0 44-45 0.0 0.0 0.0 0.07179703320968503 0.0 46-47 0.0 0.0 0.0 0.09994670022065955 0.0 48-49 0.0 0.0 0.0 0.13544143061837743 0.0 50-51 0.0 0.0 0.0 0.15370794951211092 0.0 52-53 0.0 0.0 0.0 0.18574165527733189 0.0 54-55 0.0 0.0 0.0 0.2325039436452896 0.0 56-57 0.0 0.0 0.0 0.3136841991919661 0.0 58-59 0.0 0.0 0.0 0.41739957067912226 0.0 60-61 0.0 0.0 0.0 0.538112418694763 0.0 62-63 0.0 0.0 0.0 0.6704773829731432 0.0 64-65 0.0 0.0 0.0 0.816647989952261 0.0 66-67 0.0 0.0 0.0 0.9983902389875965 0.0 68-69 0.0 0.0 0.0 1.2273562463418894 0.0 70-71 0.0 0.0 0.0 1.3782184644895028 0.0 72-73 0.0 0.0 0.0 1.548039406457349 0.0 74-75 0.0 0.0 0.0 1.7553932377731614 0.0 76-77 0.0 0.0 0.0 1.9832055702499551 0.0 78-79 0.0 0.0 0.0 2.22101641833174 0.0 80-81 0.0 0.0 0.0 2.5495061117849427 0.0 82-83 0.0 0.0 0.0 2.9000694512276253 0.0 84-85 0.0 0.0 0.0 3.3360816432637312 0.0 86-87 0.0 0.0 0.0 3.909304234063712 0.0 88-89 0.0 0.0 0.0 4.528135438354152 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 11045 0.0 25.074186 2 GTACATG 11050 0.0 25.067661 1 GTATCAA 19680 0.0 24.697788 1 ACATGGG 11080 0.0 23.966028 3 ATGGGAG 2555 0.0 23.240376 5 AGTACTT 12335 0.0 22.605959 12-13 CATGGGG 2715 0.0 22.220709 4 GAGTACT 12020 0.0 22.210373 12-13 CATGGGA 8185 0.0 21.357635 4 TATCAAC 23260 0.0 20.892477 2 GTACTTT 12730 0.0 20.85646 14-15 ATCAACG 23475 0.0 20.70113 3 TCAACGC 23570 0.0 20.617693 4 GGACCGA 185 6.5579425E-6 20.53882 6 CATGGGT 835 0.0 20.480476 4 CAACGCA 23770 0.0 20.464201 5 AACGCAG 24270 0.0 20.117802 6 TACTTTT 13850 0.0 20.027203 14-15 TACCTGG 980 0.0 19.873838 2 GGTATCA 14690 0.0 19.761822 1 >>END_MODULE