FastQCFastQC Report
Thu 26 May 2016
SRR937625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230012
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112410.9138935229900197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71850.5841406425303168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52850.42967060483962755No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45570.37048419039814245No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21600.17560804284836246No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20790.16902274124154887No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19590.15926673886108428No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17970.1460961356474571No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17750.1443075352110386No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16480.1339824326917136No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15460.12568983066831868No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14180.11528342812915646No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14160.11512082808948206No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12360.1004868245187852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG459.6050435E-442.2403264
ATAGCGC753.0752466E-431.6724978
ATCGCGT300.003949441531.6705624-25
ACCGTGC1056.8975605E-527.1478548
GTACATG115150.025.1446531
GTATCAA171850.025.037521
TACATGG114600.025.007232
TACCTGG8950.024.9578822
GGACCGA1401.7770746E-523.7543726
ACATGGG116800.023.4744283
GAGTACT80500.023.1885112-13
AGTACTT83950.023.05595412-13
ATGGGAT30550.021.9315815
CATGGGA86500.021.864864
TATCAAC196550.021.8105372
GTACTTT85600.021.80739814-15
TCGTATG2405.8917067E-921.7828312
ATCAACG196850.021.6289123
TCAACGC197550.021.6253134
CAACGCA198900.021.4776615
Produced by FastQC (version 0.11.5)