##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937625_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1230012 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.963112554999462 33.0 31.0 34.0 30.0 34.0 2 32.060490466759674 33.0 31.0 34.0 30.0 34.0 3 32.08262683616095 34.0 31.0 34.0 30.0 34.0 4 35.67528528176961 37.0 35.0 37.0 35.0 37.0 5 35.691163988644014 37.0 35.0 37.0 35.0 37.0 6 35.769777855825794 37.0 35.0 37.0 35.0 37.0 7 35.71944663954498 37.0 35.0 37.0 35.0 37.0 8 35.71814827822818 37.0 35.0 37.0 35.0 37.0 9 37.3527250140649 39.0 37.0 39.0 35.0 39.0 10-11 37.25642107556674 39.0 37.0 39.0 34.0 39.0 12-13 37.146793689817656 39.0 37.0 39.0 34.0 39.0 14-15 38.27831110590791 40.0 38.0 41.0 33.5 41.0 16-17 38.25422069052985 40.0 38.0 41.0 34.0 41.0 18-19 38.27696396457921 40.0 38.0 41.0 33.5 41.0 20-21 38.2353164034172 40.0 38.0 41.0 34.0 41.0 22-23 38.05206737820444 40.0 38.0 41.0 33.5 41.0 24-25 37.89588312959549 40.0 38.0 41.0 33.0 41.0 26-27 37.82691388376699 40.0 37.5 41.0 33.0 41.0 28-29 37.864624897968476 40.0 38.0 41.0 33.0 41.0 30-31 37.653967197068 40.0 37.0 41.0 33.0 41.0 32-33 37.37387521422555 39.5 36.0 41.0 33.0 41.0 34-35 37.350704708571946 40.0 36.0 41.0 33.0 41.0 36-37 37.24421387758818 40.0 36.0 41.0 32.5 41.0 38-39 37.08802637697843 40.0 36.0 41.0 32.0 41.0 40-41 36.831494326884616 39.5 36.0 41.0 31.0 41.0 42-43 36.530297265392534 39.0 35.0 41.0 30.5 41.0 44-45 36.17836452002094 39.0 35.0 41.0 30.0 41.0 46-47 35.66220898657899 39.0 35.0 41.0 28.0 41.0 48-49 35.18549371876047 39.0 35.0 41.0 25.0 41.0 50-51 34.18744695173706 38.0 34.0 39.5 23.5 40.5 52-53 34.256084899984714 38.0 34.5 39.5 23.0 40.5 54-55 34.61527855012797 39.0 35.0 40.0 23.0 41.0 56-57 34.4389757986101 38.5 34.5 40.0 22.5 41.0 58-59 34.22188767264059 38.0 34.0 40.0 21.5 41.0 60-61 33.91178622647584 38.0 34.0 40.0 19.5 41.0 62-63 33.58694183471381 38.0 34.0 40.0 18.0 41.0 64-65 33.23543266244557 37.0 33.0 40.0 15.5 41.0 66-67 32.834284137065325 36.5 33.0 39.5 10.5 41.0 68-69 32.368701280963116 36.0 32.0 39.0 7.5 41.0 70-71 31.930610839569045 35.5 32.0 39.0 6.0 40.0 72-73 31.48687736379808 35.0 31.0 38.0 2.0 40.0 74-75 31.0423995863455 35.0 31.0 37.0 2.0 39.5 76-77 30.5721623041076 35.0 31.0 37.0 2.0 39.0 78-79 30.06120224843335 34.5 30.0 36.0 2.0 39.0 80-81 29.552684038854906 34.0 29.5 36.0 2.0 37.5 82-83 29.049973496193534 34.0 29.0 35.0 2.0 37.0 84-85 28.608090815374158 34.0 29.0 35.0 2.0 37.0 86-87 28.169934927464123 34.0 28.0 35.0 2.0 36.0 88-89 27.705215884072675 34.0 27.0 35.0 2.0 36.0 90-91 27.245132974312448 33.5 26.0 35.0 2.0 35.5 92-93 26.732967645844106 33.0 25.0 35.0 2.0 35.0 94-95 26.150630644253877 33.0 24.5 35.0 2.0 35.0 96-97 25.475147803436066 32.0 21.5 35.0 2.0 35.0 98-99 24.62857963987343 32.0 10.0 34.0 2.0 35.0 100-101 22.322748070750528 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28590789206620215 1101 2 0.22792493328879715 1101 3 0.1857489474593379 1101 4 0.18412144491287563 1101 5 0.15034362176840688 1101 6 0.20853771854218905 1101 7 0.2371282006116644 1101 8 0.1876426402360991 1101 9 0.34255463890753646 1101 10-11 0.17706480545142256 1101 12-13 0.3477076936641694 1101 14-15 0.5015256592504684 1101 16-17 0.4355999421004526 1101 18-19 0.5578196758720466 1101 20-21 0.5658185562161648 1101 22-23 0.5462407714958957 1101 24-25 0.6012258307913712 1101 26-27 0.4725423461710321 1101 28-29 0.5354423129695434 1101 30-31 0.448577546341717 1101 32-33 0.34673209604028443 1101 34-35 0.5169564154274084 1101 36-37 0.552077839698228 1101 38-39 0.6279995171780044 1101 40-41 0.6095613768650523 1101 42-43 0.5214882544037565 1101 44-45 0.61004796072136 1101 46-47 0.7083848813017539 1101 48-49 1.0028239124539056 1101 50-51 0.906320690970496 1101 52-53 0.9789233085836457 1101 54-55 0.9875573533511499 1101 56-57 0.9450742642843935 1101 58-59 1.0165765427053657 1101 60-61 1.0620049256610002 1101 62-63 1.1999703575706704 1101 64-65 0.8839303536677079 1101 66-67 1.0863988431799854 1101 68-69 0.9618378936811283 1101 70-71 1.0463941291989336 1101 72-73 1.1877871675122726 1101 74-75 1.1399210522012133 1101 76-77 1.2233679284011743 1101 78-79 1.1832316281866433 1101 80-81 1.036193013021606 1101 82-83 1.090011879864381 1101 84-85 1.4380919793420972 1101 86-87 1.5066787037102927 1101 88-89 1.3806179138394121 1101 90-91 1.396687720338548 1101 92-93 1.571459453989707 1101 94-95 1.7908170684672307 1101 96-97 1.9541947075814328 1101 98-99 2.013890537620437 1101 100-101 2.6969700154968272 1102 1 0.1995181204694454 1102 2 0.16935483263939943 1102 3 0.20284366036502632 1102 4 0.22082630067235698 1102 5 0.08812065137173875 1102 6 0.037052915758984284 1102 7 0.10718409752180946 1102 8 0.14867624323419193 1102 9 0.05666169807143007 1102 10-11 0.20672953897636148 1102 12-13 0.1968327205133633 1102 14-15 0.3002468788729473 1102 16-17 0.3006822420561548 1102 18-19 0.2206734125204619 1102 20-21 0.283241116711352 1102 22-23 0.3476158640019875 1102 24-25 0.285810508883948 1102 26-27 0.22848676846154348 1102 28-29 0.15119279556505916 1102 30-31 0.31200612013571316 1102 32-33 0.3052035848445769 1102 34-35 0.28393487766215486 1102 36-37 0.43507033204362244 1102 38-39 0.3675336222042205 1102 40-41 0.4650720266223729 1102 42-43 0.5308653485831272 1102 44-45 0.6168266051856364 1102 46-47 0.5932185267066288 1102 48-49 0.5122387219844811 1102 50-51 0.5152881596403134 1102 52-53 0.5456093388635566 1102 54-55 0.7602278439864776 1102 56-57 0.8036975803744539 1102 58-59 0.6812514248793846 1102 60-61 0.8998084479988364 1102 62-63 0.931600106887764 1102 64-65 0.9865807309822046 1102 66-67 1.0400351779653647 1102 68-69 1.0142419943516856 1102 70-71 1.1034227298536088 1102 72-73 1.1324237916019833 1102 74-75 1.055001259414869 1102 76-77 1.0071736465083632 1102 78-79 0.8651608235940209 1102 80-81 0.83130045807793 1102 82-83 0.8673484953531556 1102 84-85 1.1207018908444084 1102 86-87 1.1978136644150297 1102 88-89 1.3227414076975563 1102 90-91 1.2161799797226536 1102 92-93 1.526840813683311 1102 94-95 1.6948211509240245 1102 96-97 1.827326935303045 1102 98-99 2.0206015704185383 1102 100-101 2.8202401112248268 1103 1 0.3863034089836752 1103 2 0.34278325052550684 1103 3 0.2800388984308455 1103 4 0.21086413035846618 1103 5 0.1577680419881773 1103 6 0.21177144330441422 1103 7 0.26872356367054806 1103 8 0.21718794908213113 1103 9 0.2759490139638672 1103 10-11 0.3050542936393299 1103 12-13 0.2723334763128946 1103 14-15 0.5105541667571174 1103 16-17 0.39285470906879283 1103 18-19 0.4033220994235549 1103 20-21 0.2862579172570463 1103 22-23 0.3201218358331275 1103 24-25 0.37908203990526346 1103 26-27 0.33652220147335044 1103 28-29 0.13292719887169113 1103 30-31 0.18154091294604058 1103 32-33 0.27630230470635553 1103 34-35 0.3227566333937375 1103 36-37 0.3444196120265417 1103 38-39 0.5070384212886125 1103 40-41 0.5438302651902944 1103 42-43 0.35830523979741 1103 44-45 0.35654874627351774 1103 46-47 0.664953741846972 1103 48-49 0.6811091789145038 1103 50-51 0.6772778846492855 1103 52-53 0.6397970507014179 1103 54-55 0.7516689547161874 1103 56-57 0.6858727123194939 1103 58-59 0.8012836864451529 1103 60-61 0.8369056747925256 1103 62-63 0.9561962416768708 1103 64-65 0.9934396228115361 1103 66-67 0.9200538766135722 1103 68-69 1.137462688698939 1103 70-71 1.2612493804130303 1103 72-73 1.0087971803988616 1103 74-75 1.1519326603486597 1103 76-77 1.130562443379624 1103 78-79 1.1939634041663112 1103 80-81 1.2044818843815186 1103 82-83 1.1620924456477546 1103 84-85 1.372501161429465 1103 86-87 1.3769304334580497 1103 88-89 1.2944715047558084 1103 90-91 1.4771362051194608 1103 92-93 1.473969922598748 1103 94-95 1.615748106185812 1103 96-97 1.731551294373567 1103 98-99 2.0481500885131645 1103 100-101 2.442046494179145 1104 1 0.08199547360501569 1104 2 0.3137660141642229 1104 3 0.2550889074394007 1104 4 0.236245607853256 1104 5 0.16934610916887038 1104 6 0.09253287376994734 1104 7 0.12541044952989466 1104 8 0.08518726535618981 1104 9 0.1839273564828403 1104 10-11 0.3053571195525393 1104 12-13 0.41334063233637863 1104 14-15 0.5002118880094457 1104 16-17 0.5337874867978059 1104 18-19 0.44693988738536206 1104 20-21 0.4336357358941214 1104 22-23 0.5175742637531684 1104 24-25 0.4346460907042555 1104 26-27 0.4236619171727156 1104 28-29 0.4137253565074843 1104 30-31 0.5069907630345085 1104 32-33 0.39136275439474844 1104 34-35 0.40139920642074856 1104 36-37 0.5874788698143192 1104 38-39 0.6637973755575146 1104 40-41 0.6330826016388968 1104 42-43 0.6360712114966987 1104 44-45 0.6509699122110248 1104 46-47 0.6748211082547755 1104 48-49 0.6540528038631024 1104 50-51 0.5450653844760467 1104 52-53 0.547354994164678 1104 54-55 0.6840643977752094 1104 56-57 0.6399092362198715 1104 58-59 0.7420111154046438 1104 60-61 0.7304876595380421 1104 62-63 0.7975267815893758 1104 64-65 0.6592761938335698 1104 66-67 0.7067122122952938 1104 68-69 0.7952225161043316 1104 70-71 0.6965878478505836 1104 72-73 0.7666059773645948 1104 74-75 0.8238049606706532 1104 76-77 1.0173870907756921 1104 78-79 1.0616150827596833 1104 80-81 1.1164332289655299 1104 82-83 1.2024948737987806 1104 84-85 1.2962627166287817 1104 86-87 1.3343557895268106 1104 88-89 1.2714525775587617 1104 90-91 1.1419721339934839 1104 92-93 1.1985759717253792 1104 94-95 1.1953887804874164 1104 96-97 1.1695142530589635 1104 98-99 1.4300918701736016 1104 100-101 1.82550818011795 1105 1 0.21615374597098835 1105 2 0.21822144099456864 1105 3 0.22393544609770544 1105 4 0.1553120322465844 1105 5 0.1127116084039983 1105 6 0.298862558722206 1105 7 0.3132077363037382 1105 8 0.3080972167923193 1105 9 0.24913248656194753 1105 10-11 0.3529139307179534 1105 12-13 0.4527938036053172 1105 14-15 0.3647355613235064 1105 16-17 0.40533918703747673 1105 18-19 0.45095176247514956 1105 20-21 0.47698636606521205 1105 22-23 0.38082549801148957 1105 24-25 0.3529824188519015 1105 26-27 0.40448536089834874 1105 28-29 0.40037167691816933 1105 30-31 0.36827398846212134 1105 32-33 0.3948527001352673 1105 34-35 0.43818098652493376 1105 36-37 0.4386503756472777 1105 38-39 0.4332994131233221 1105 40-41 0.5564620626731269 1105 42-43 0.5359973702126197 1105 44-45 0.5473693305534013 1105 46-47 0.4046683601241199 1105 48-49 0.31367170219147056 1105 50-51 0.09176231010804514 1105 52-53 0.20501924061814947 1105 54-55 0.2270901766999458 1105 56-57 0.2777091691830762 1105 58-59 0.49507351254978005 1105 60-61 0.3546776105709455 1105 62-63 0.4252054182726184 1105 64-65 0.4112443286732983 1105 66-67 0.4415899961495029 1105 68-69 0.4790074237523285 1105 70-71 0.6055788432462101 1105 72-73 0.6709295987629424 1105 74-75 0.4478613421453126 1105 76-77 0.5832648569886416 1105 78-79 0.6489179101500113 1105 80-81 0.706202556858635 1105 82-83 0.8090188646555667 1105 84-85 0.7192618824490893 1105 86-87 0.8315533489841656 1105 88-89 0.7764309418105064 1105 90-91 0.9491365026757066 1105 92-93 1.2422774110003765 1105 94-95 1.2275621588143686 1105 96-97 0.9421088039250236 1105 98-99 1.4634262955850836 1105 100-101 1.8707491975651607 1106 1 0.21017040961949718 1106 2 0.13807538854897672 1106 3 0.21359176757203358 1106 4 0.2547381655750769 1106 5 0.21965154058906222 1106 6 -0.02620915332377649 1106 7 0.16391611450736576 1106 8 0.06891646837571841 1106 9 0.21349473613746994 1106 10-11 0.07107409452739688 1106 12-13 0.12866074873743827 1106 14-15 0.028450202729224827 1106 16-17 0.038125826599362256 1106 18-19 0.2396278726707166 1106 20-21 0.32153202136259296 1106 22-23 0.15313457196246105 1106 24-25 0.09372286524057927 1106 26-27 0.20952226286983944 1106 28-29 0.3280840044643938 1106 30-31 0.497223969700336 1106 32-33 0.3042866163424449 1106 34-35 0.3312535432772208 1106 36-37 0.35529381478272626 1106 38-39 0.39455276881563606 1106 40-41 0.5347193904151979 1106 42-43 0.37426052234092566 1106 44-45 0.6361233830716557 1106 46-47 0.44464232636651957 1106 48-49 0.2633495159916279 1106 50-51 0.21673995774857246 1106 52-53 0.36122036700676574 1106 54-55 0.3908394465228753 1106 56-57 0.45163834214861254 1106 58-59 0.46202125599749166 1106 60-61 0.3878561756942247 1106 62-63 0.6789034210076963 1106 64-65 0.6399017281699955 1106 66-67 0.5142696509325688 1106 68-69 0.6499633781086658 1106 70-71 0.5870597360235799 1106 72-73 0.5760812286544201 1106 74-75 0.8080147630639409 1106 76-77 0.844657464332478 1106 78-79 0.6117516840968378 1106 80-81 0.6558485806364374 1106 82-83 0.576679783194205 1106 84-85 0.7547585646493431 1106 86-87 1.1093694766301851 1106 88-89 0.9036656810386567 1106 90-91 1.1540785454911884 1106 92-93 1.0522429017957435 1106 94-95 1.2441156776008562 1106 96-97 1.3770261129677515 1106 98-99 1.463785068694584 1106 100-101 2.0000099830398455 1107 1 0.09764203930047799 1107 2 0.17752581092896946 1107 3 0.15323358234887507 1107 4 0.23055358018267924 1107 5 0.1789449300941257 1107 6 0.12946207110705643 1107 7 0.18401013204407235 1107 8 0.1977534428656753 1107 9 0.16928477736764336 1107 10-11 0.24573073332245343 1107 12-13 0.26445668088456387 1107 14-15 0.3975323741561496 1107 16-17 0.4166219204346788 1107 18-19 0.42507381321114934 1107 20-21 0.45260648342640053 1107 22-23 0.44211181767173713 1107 24-25 0.4023719669069479 1107 26-27 0.4834367958992303 1107 28-29 0.4135076044511692 1107 30-31 0.3660580860971123 1107 32-33 0.37271641864102634 1107 34-35 0.48355683563831775 1107 36-37 0.3602199692682788 1107 38-39 0.3520941721643496 1107 40-41 0.4524581020392091 1107 42-43 0.5249303957299034 1107 44-45 0.566727570240559 1107 46-47 0.6961685668444844 1107 48-49 0.8001777314868619 1107 50-51 0.764869185994776 1107 52-53 0.654048238033127 1107 54-55 0.7862859674184648 1107 56-57 0.9089585240930731 1107 58-59 0.9055321052068663 1107 60-61 1.0395850412496443 1107 62-63 1.0734513274544426 1107 64-65 1.064429659166393 1107 66-67 1.2294803229776008 1107 68-69 1.1950978488965163 1107 70-71 1.395711021822283 1107 72-73 1.4538081958868183 1107 74-75 1.2460156704149163 1107 76-77 1.3127148408650342 1107 78-79 1.2837471136439085 1107 80-81 1.2417517536814984 1107 82-83 1.16435834381355 1107 84-85 1.2646870062334798 1107 86-87 1.4813827536586714 1107 88-89 1.4210357735273007 1107 90-91 1.588845064864131 1107 92-93 1.6326610944948747 1107 94-95 2.0288123359407777 1107 96-97 1.8101086663058226 1107 98-99 1.9559187600592125 1107 100-101 2.305989450947763 1108 1 0.0867086042731593 1108 2 0.06692694114320119 1108 3 0.1389990858572503 1108 4 0.02569010503015079 1108 5 0.02344097580849791 1108 6 0.14959322174660628 1108 7 0.038782728175839054 1108 8 0.21520248106547513 1108 9 0.10114181744692274 1108 10-11 0.1261005320708506 1108 12-13 0.04352382487406459 1108 14-15 0.19855656840764624 1108 16-17 0.322740996909495 1108 18-19 0.3208893850368568 1108 20-21 0.23757427478334847 1108 22-23 0.2618482385624006 1108 24-25 0.358874159517228 1108 26-27 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-0.6214075034444733 2316 54-55 -0.7746391545886837 2316 56-57 -0.6904971208248654 2316 58-59 -0.514178135347386 2316 60-61 -0.7298590567423133 2316 62-63 -0.7552196495281507 2316 64-65 -0.29152626134892046 2316 66-67 -0.3048003679067577 2316 68-69 -0.28670789720790424 2316 70-71 -0.3821518715074532 2316 72-73 -0.3870602777865919 2316 74-75 -0.5565379302545601 2316 76-77 -0.695991219460609 2316 78-79 -0.5114578945837813 2316 80-81 -0.4528300424086389 2316 82-83 -0.46071216485469435 2316 84-85 -0.5370422163405593 2316 86-87 -0.4164466656556769 2316 88-89 -0.5014413036117986 2316 90-91 -0.4831743597207634 2316 92-93 -0.39613460538160794 2316 94-95 -0.5425883021576432 2316 96-97 -0.01407671214769124 2316 98-99 -0.1942286945879239 2316 100-101 -0.25303505508922797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4123.0 3 654.0 4 552.0 5 597.0 6 697.0 7 883.0 8 995.0 9 1356.0 10 1876.0 11 2706.0 12 4275.0 13 6351.0 14 7318.0 15 10234.0 16 14130.0 17 20475.0 18 16672.0 19 8978.0 20 7926.0 21 7881.0 22 8466.0 23 8865.0 24 10084.0 25 11433.0 26 12989.0 27 15449.0 28 18468.0 29 22289.0 30 27536.0 31 34461.0 32 44087.0 33 58562.0 34 80777.0 35 116100.0 36 168975.0 37 242900.0 38 203210.0 39 26672.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.39754753285719 19.538531780973813 14.524752959592997 20.539167726576004 2 14.66698621773003 21.56537631621208 41.58133756575456 22.18629990030333 3 20.111259184995905 27.655691539003886 29.328247199162092 22.90480207683812 4 11.951844462699997 16.68141780437872 38.48302885681127 32.88370887611 5 12.413501401336385 39.614259646972435 33.00764520884761 14.964593742843569 6 29.313242382597547 37.087501863466365 18.27476007106833 15.32449568286776 7 27.30870895087749 35.23779268436723 20.406958037543426 17.046540327211858 8 26.250672097759676 33.973523421588595 19.94582484725051 19.82997963340122 9 27.828070518460585 17.54154853846906 21.199449278195978 33.43093166487437 10-11 24.427101557723258 28.25988042831381 26.383573618626038 20.92944439533689 12-13 26.391485080356908 25.912541217156114 27.604404914897646 20.091568787589335 14-15 23.23098072360315 26.243678910012928 28.109202366526482 22.416137999857437 16-17 20.258448283867878 30.156440800712307 28.249401046142868 21.335709869276943 18-19 20.081226701597977 28.104878652798416 32.31089192148276 19.50300272412085 20-21 21.802217726909333 26.530373703315597 32.00174961523533 19.665658954539733 22-23 20.909588478773987 26.913444199559205 31.915537398211093 20.261429923455715 24-25 20.580750439718727 27.05193588231054 32.41595457142543 19.9513591065453 26-27 20.497763376061954 26.915650176783508 32.725374023068 19.861212424086546 28-29 19.922044139685106 27.714249286857445 32.57115437647328 19.79255219698417 30-31 20.298928760539287 27.379821595861674 32.313469919758866 20.00777972384017 32-33 19.99395522302946 27.446057290147664 32.31198055243722 20.248006934385657 34-35 20.515317622658692 27.338211250980592 32.25470094242868 19.891770183932035 36-37 20.5851916680683 27.073402270604923 32.309713541486374 20.031692519840405 38-39 20.297961973011475 27.61065954661821 32.29035918179174 19.80101929857857 40-41 20.302987110830568 27.761682718739184 32.00631223147564 19.929017938954612 42-43 20.010393846126277 27.905277524244504 31.48178694326027 20.60254168636895 44-45 20.41564697578929 27.837041688913544 31.104898955404842 20.642412379892328 46-47 20.823796483488557 28.208006229565346 30.36615801657416 20.602039270371932 48-49 20.770031590454764 28.412469676258933 30.090763498443284 20.726735234843016 50-51 20.786125990346036 28.794191327725617 29.984928409947248 20.4347542719811 52-53 20.74795954368088 29.257988477135566 29.401718733576743 20.592333245606817 54-55 20.491570253588865 28.849836716174508 29.551602820749466 21.10699020948716 56-57 20.925899307819133 29.228486465642806 29.005143206394823 20.84047102014324 58-59 21.212912179085976 28.676266685778206 29.352717428705343 20.758103706430475 60-61 20.927679339705893 28.792291030459356 29.87079095288262 20.409238676952135 62-63 20.843519761772985 28.76081308977944 29.8436594497272 20.552007698720367 64-65 20.637952090068424 29.034996882804442 29.791349466737632 20.535701560389505 66-67 20.706857015569522 28.934803315318952 29.290109140990644 21.06823052812088 68-69 20.60575652777348 29.016439775394748 29.441443481348866 20.936360215482903 70-71 20.821763062850646 28.986006539213495 29.2840135410342 20.908216856901664 72-73 20.6985959909774 28.764612961906426 29.399485504829553 21.137305542286626 74-75 20.425391894593968 29.640560179282133 28.965645872182073 20.96840205394183 76-77 20.542669915783893 29.14141276319825 29.12491737030039 21.19099995071747 78-79 20.533037681008302 29.027882773092816 29.15223433650926 21.286845209389625 80-81 20.64318246704242 29.369911373232284 28.852755591558527 21.134150568166778 82-83 20.703913931864086 29.077075146302555 29.049217690136302 21.16979323169706 84-85 20.590490719469667 28.94441374260492 28.936390992202455 21.528704545722956 86-87 20.5469036661287 29.1023170936819 28.909609441208893 21.441169798980507 88-89 20.69601418272765 28.73888003531835 28.833162755596792 21.73194302635721 90-91 20.466986478390716 28.55347981573697 29.15199663761916 21.827537068253157 92-93 20.617417641469824 28.858341006730733 29.15836711189341 21.365874239906038 94-95 20.391662560121855 28.888382075644593 29.061223492422915 21.65873187181064 96-97 20.054607091592096 29.04698205214346 29.10505044335176 21.793360412912683 98-99 20.31104410605261 29.126797570876583 28.62430035641913 21.93785796665168 100-101 20.522338980773185 28.68649684303972 29.08891001899674 21.702254157190357 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1956.0 1 1201.5 2 528.5 3 636.0 4 578.0 5 571.0 6 949.5 7 1776.0 8 2774.5 9 3252.0 10 3035.0 11 2902.5 12 3005.5 13 3158.0 14 3349.0 15 3460.5 16 3589.0 17 3706.0 18 3853.0 19 4117.0 20 4492.5 21 4991.5 22 5722.0 23 6576.0 24 7538.0 25 8879.5 26 10727.5 27 12941.5 28 14722.0 29 16394.5 30 18687.0 31 21120.0 32 23863.5 33 26801.5 34 30198.5 35 33470.5 36 36939.0 37 40167.0 38 40708.5 39 40269.0 40 40083.5 41 40242.0 42 40523.5 43 41560.5 44 43417.5 45 43874.0 46 43747.5 47 46622.5 48 55652.5 49 54090.5 50 42897.0 51 36671.5 52 32514.5 53 30455.0 54 28505.5 55 25559.5 56 22610.5 57 20085.0 58 17807.5 59 15611.5 60 13358.5 61 11323.5 62 8950.5 63 6137.0 64 3958.0 65 2842.0 66 2048.0 67 1492.0 68 1124.5 69 843.5 70 660.0 71 501.0 72 372.0 73 321.5 74 264.5 75 195.0 76 142.5 77 91.0 78 61.0 79 44.5 80 37.0 81 27.0 82 12.0 83 11.0 84 8.0 85 3.5 86 3.0 87 3.5 88 2.0 89 0.5 90 1.5 91 1.5 92 2.0 93 1.5 94 2.0 95 2.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2838996692715193 2 0.26755836528424115 3 0.25585116242768363 4 0.24292445927356804 5 0.24137975889666116 6 0.20007934881936112 7 0.19243714695466385 8 0.20422564983105856 9 0.20585165022780266 10-11 0.2071118005352793 12-13 0.20483539997983757 14-15 0.20117689908716335 16-17 0.1995915487003379 18-19 0.1999980487995239 20-21 0.1880875958933734 22-23 0.18097384415761797 24-25 0.18199009440558303 26-27 0.1935346972224661 28-29 0.2050793000393492 30-31 0.20016064883919832 32-33 0.20373784971203535 34-35 0.1982907483829426 36-37 0.185160795179234 38-39 0.18739654572475717 40-41 0.1818681443758272 42-43 0.19207129686539642 44-45 0.18792499585369898 46-47 0.18845344598264083 48-49 0.19723384812505895 50-51 0.20625815032698866 52-53 0.22056695381833671 54-55 0.19471354751010558 56-57 0.16922599129114188 58-59 0.17337229230283932 60-61 0.17849419355258322 62-63 0.18499819513955962 64-65 0.17442919256072298 66-67 0.17857549357242045 68-69 0.18357544479240853 70-71 0.17792509341372278 72-73 0.17938849377079247 74-75 0.18101449416753657 76-77 0.1948354975398614 78-79 0.19869724848212864 80-81 0.18987619632979189 82-83 0.1897135962901175 84-85 0.1832095947031411 86-87 0.18255919454444347 88-89 0.18873799605207103 90-91 0.1878436958338618 92-93 0.18581119533793167 94-95 0.1867461455660595 96-97 0.1747543926400718 98-99 0.18243724451468768 100-101 0.1763803930368159 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1230012.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.43378954792453 #Duplication Level Percentage of deduplicated Percentage of total 1 78.362926328604 45.00679819116556 2 12.832873724220958 14.740811375439941 3 3.9991994242366435 6.890675342753649 4 1.6644822915431616 3.8239010255494845 5 0.8671487261462768 2.490181872211805 6 0.4931496518526213 1.699407199208142 7 0.31841394532317113 1.280140366737075 8 0.2224789695472621 1.022224825265325 9 0.16097783991068346 0.8321010641378712 >10 0.9589888252232753 10.27025962042801 >50 0.07734064080202517 3.0753160400054025 >100 0.03664339444266324 3.7260587798954714 >500 0.002971078976108004 1.1972450206216358 >1k 0.001980719317405336 2.0181874967544164 >5k 2.829599024864766E-4 1.0132784991958599 >10k+ 1.414799512432383E-4 0.9134132806303659 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11241 0.9138935229900197 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7185 0.5841406425303168 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5285 0.42967060483962755 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4557 0.37048419039814245 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2160 0.17560804284836246 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2079 0.16902274124154887 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1959 0.15926673886108428 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1797 0.1460961356474571 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1775 0.1443075352110386 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1648 0.1339824326917136 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1546 0.12568983066831868 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1418 0.11528342812915646 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1416 0.11512082808948206 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1236 0.1004868245187852 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.130001983720483E-5 0.0 16-17 0.0 0.0 0.0 1.2195002975580726E-4 0.0 18-19 0.0 0.0 0.0 1.6260003967440967E-4 0.0 20-21 0.0 0.0 0.0 2.0325004959301208E-4 0.0 22-23 0.0 0.0 0.0 3.252000793488194E-4 0.0 24-25 0.0 0.0 0.0 5.284501289418315E-4 0.0 26-27 0.0 0.0 0.0 8.130001983720484E-4 0.0 28-29 0.0 0.0 0.0 0.0025609506248719526 0.0 30-31 0.0 0.0 0.0 0.005731651398522941 0.0 32-33 0.0 0.0 0.0 0.009756002380464581 0.0 34-35 0.0 0.0 0.0 0.013821003372324822 0.0 36-37 0.0 0.0 0.0 0.019918504860115184 0.0 38-39 0.0 0.0 0.0 0.02987775729017278 0.0 40-41 0.0 0.0 0.0 0.042276010315346516 0.0 42-43 0.0 0.0 0.0 0.059349014481159534 0.0 44-45 0.0 0.0 0.0 0.07438951815104242 0.0 46-47 0.0 0.0 0.0 0.10479572557015704 0.0 48-49 0.0 0.0 0.0 0.140689684328283 0.0 50-51 0.0 0.0 0.0 0.16052688916856095 0.0 52-53 0.0 0.0 0.0 0.19487614754978 0.0 54-55 0.0 0.0 0.0 0.24219275909503324 0.0 56-57 0.0 0.0 0.0 0.3222326286247614 0.0 58-59 0.0 0.0 0.0 0.4265812040858138 0.0 60-61 0.0 0.0 0.0 0.5527181848632372 0.0 62-63 0.0 4.065000991860242E-5 0.0 0.6858469673466601 0.0 64-65 0.0 8.130001983720483E-5 0.0 0.8310488027759079 0.0 66-67 0.0 8.130001983720483E-5 0.0 1.0190144486395254 0.0 68-69 0.0 8.130001983720483E-5 0.0 1.255434906326117 0.0 70-71 0.0 8.130001983720483E-5 0.0 1.4042545926381207 0.0 72-73 0.0 8.130001983720483E-5 0.0 1.5747000842268206 0.0 74-75 0.0 8.130001983720483E-5 0.0 1.776689983512356 0.0 76-77 0.0 8.130001983720483E-5 0.0 1.9981105875389833 0.0 78-79 0.0 8.130001983720483E-5 0.0 2.2298969440948544 0.0 80-81 0.0 8.130001983720483E-5 0.0 2.5534303730370107 0.0 82-83 0.0 1.2195002975580726E-4 0.0 2.9077765094974684 0.0 84-85 0.0 1.6260003967440967E-4 0.0 3.358016019355909 0.0 86-87 0.0 1.6260003967440967E-4 0.0 3.9565467653974107 0.0 88-89 0.0 1.6260003967440967E-4 0.0 4.596784421615399 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGCG 45 9.6050435E-4 42.240326 4 ATAGCGC 75 3.0752466E-4 31.672497 8 ATCGCGT 30 0.0039494415 31.67056 24-25 ACCGTGC 105 6.8975605E-5 27.147854 8 GTACATG 11515 0.0 25.144653 1 GTATCAA 17185 0.0 25.03752 1 TACATGG 11460 0.0 25.00723 2 TACCTGG 895 0.0 24.957882 2 GGACCGA 140 1.7770746E-5 23.754372 6 ACATGGG 11680 0.0 23.474428 3 GAGTACT 8050 0.0 23.18851 12-13 AGTACTT 8395 0.0 23.055954 12-13 ATGGGAT 3055 0.0 21.931581 5 CATGGGA 8650 0.0 21.86486 4 TATCAAC 19655 0.0 21.810537 2 GTACTTT 8560 0.0 21.807398 14-15 TCGTATG 240 5.8917067E-9 21.782831 2 ATCAACG 19685 0.0 21.628912 3 TCAACGC 19755 0.0 21.625313 4 CAACGCA 19890 0.0 21.477661 5 >>END_MODULE