FastQCFastQC Report
Thu 26 May 2016
SRR937625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230012
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151011.2277115995616303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101660.8264960016650243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78880.6412945564758717No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44450.3613785881763755No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26910.21877835338191826No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23140.188128245903292No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21090.171461741836665No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20310.16512034028936304No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20210.164307340090991No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19660.15983583899994472No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14910.12121832957727241No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13380.10877942654218009No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12900.10487702558999423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAC404.496783E-429.68561780-81
GTATCAA189600.025.6962361
GTACATG103550.024.4658951
TACATGG104300.023.8206772
AGTACTT118700.023.49031412-13
ACATGGG104200.023.068513
GAGTACT115000.022.84171512-13
GTACGTG1302.9283992E-421.937741
TATCAAC223350.021.822182
GTACTTT120700.021.74158114-15
ATCAACG224200.021.7182583
TCAACGC225850.021.559594
CAACGCA228400.021.3604835
CATGGGA78750.021.3545024
AACGCAG231850.021.1211556
TACTTTT131450.021.10174614-15
GTATAGG3900.020.7189771
TACACCG1150.00372128320.6542245
GGTATCA139100.020.2291951
ATGGGAG24550.020.1241975
Produced by FastQC (version 0.11.5)