##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937625_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1230012 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25742187881094 33.0 31.0 34.0 31.0 34.0 2 32.44612979385567 34.0 31.0 34.0 31.0 34.0 3 32.50803488096051 34.0 31.0 34.0 31.0 34.0 4 36.1128143465267 37.0 35.0 37.0 35.0 37.0 5 36.051723885620625 37.0 35.0 37.0 35.0 37.0 6 36.121837835728435 37.0 35.0 37.0 35.0 37.0 7 36.07707729680686 37.0 35.0 37.0 35.0 37.0 8 36.05807097816932 37.0 35.0 37.0 35.0 37.0 9 37.723729524590006 39.0 37.0 39.0 35.0 39.0 10-11 37.66162769143716 39.0 37.0 39.0 35.0 39.0 12-13 37.55316086347125 39.0 37.0 39.0 35.0 39.0 14-15 38.69697043606079 40.0 38.0 41.0 34.5 41.0 16-17 38.70103706305304 40.0 38.0 41.0 34.5 41.0 18-19 38.77727249815449 40.0 38.0 41.0 35.0 41.0 20-21 38.719135260469 40.0 38.0 41.0 34.5 41.0 22-23 38.66037811013226 40.0 38.0 41.0 34.0 41.0 24-25 38.6557123019938 40.0 38.0 41.0 34.0 41.0 26-27 38.451328523624156 40.0 38.0 41.0 34.0 41.0 28-29 38.382715371882554 40.0 38.0 41.0 34.0 41.0 30-31 38.289226853071355 40.0 37.5 41.0 34.0 41.0 32-33 38.05255355232307 40.0 37.0 41.0 34.0 41.0 34-35 37.84464216609268 40.0 37.0 41.0 33.0 41.0 36-37 37.622124418298355 40.0 36.0 41.0 33.0 41.0 38-39 37.422047508479594 40.0 36.0 41.0 33.0 41.0 40-41 37.17332188629054 40.0 36.0 41.0 33.0 41.0 42-43 36.83111994029326 40.0 36.0 41.0 31.5 41.0 44-45 36.31969688100604 40.0 35.0 41.0 30.5 41.0 46-47 35.69348713671086 39.0 35.0 41.0 28.0 41.0 48-49 35.12009069830213 39.0 35.0 41.0 24.5 41.0 50-51 34.77935865666352 39.0 35.0 41.0 23.0 41.0 52-53 34.498541477644125 39.0 35.0 40.0 20.5 41.0 54-55 34.27241197646852 39.0 34.5 40.0 19.5 41.0 56-57 34.01106371319955 38.0 34.0 40.0 17.5 41.0 58-59 33.77065670904024 38.0 34.0 40.0 14.0 41.0 60-61 33.42113654175732 38.0 33.5 40.0 9.5 41.0 62-63 33.033867962263784 37.5 33.0 40.0 7.5 41.0 64-65 32.652994442330645 37.0 33.0 39.5 4.0 41.0 66-67 32.235597701485844 36.0 32.0 39.0 2.0 40.5 68-69 31.775717635275104 36.0 31.5 39.0 2.0 40.0 70-71 31.351126249174804 35.0 31.0 38.5 2.0 40.0 72-73 30.88201253321106 35.0 31.0 37.5 2.0 40.0 74-75 30.418894693710307 35.0 30.0 37.0 2.0 39.0 76-77 29.15822000110568 33.5 28.5 35.5 2.0 38.5 78-79 29.732772932296598 34.0 30.0 36.0 2.0 38.5 80-81 29.557733989587092 34.0 30.0 36.0 2.0 37.5 82-83 29.169219487289553 34.0 29.5 35.0 2.0 37.0 84-85 28.769533549266185 34.0 29.0 35.0 2.0 36.5 86-87 28.452441114395633 34.0 29.0 35.0 2.0 36.0 88-89 28.054208007726757 34.0 29.0 35.0 2.0 36.0 90-91 27.568938758321057 34.0 27.0 35.0 2.0 35.0 92-93 27.15214241812275 33.0 26.0 35.0 2.0 35.0 94-95 26.706010185266486 33.0 25.5 35.0 2.0 35.0 96-97 26.226905509864945 33.0 25.0 35.0 2.0 35.0 98-99 25.497715469442575 32.5 22.0 34.5 2.0 35.0 100-101 23.191277808671785 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4157467465637552 1101 2 0.3375129360938516 1101 3 0.350531431658176 1101 4 0.2707153213691953 1101 5 0.24182886292683747 1101 6 0.12999844289655726 1101 7 0.2582819897530584 1101 8 0.26547512160991715 1101 9 0.4498490953901282 1101 10-11 0.37620014992488393 1101 12-13 0.4834164155850118 1101 14-15 0.5916047030325018 1101 16-17 0.6121711217850958 1101 18-19 0.5938733351516419 1101 20-21 0.6245216188078828 1101 22-23 0.44958354737171646 1101 24-25 0.4589687829903113 1101 26-27 0.42299933572814297 1101 28-29 0.5676911500420019 1101 30-31 0.7526185633925948 1101 32-33 0.7505623785038793 1101 34-35 0.6593698970297339 1101 36-37 0.6423723156184309 1101 38-39 0.8682307908734614 1101 40-41 0.9246680095079611 1101 42-43 0.9368973322522365 1101 44-45 0.9352944730937409 1101 46-47 1.1106492019904266 1101 48-49 0.9862032006776644 1101 50-51 1.0822944784324164 1101 52-53 1.0930572004435959 1101 54-55 1.1285971933863195 1101 56-57 1.20635671135134 1101 58-59 1.2589937399902098 1101 60-61 1.3471714985846504 1101 62-63 1.3410462559865195 1101 64-65 1.3753185383606592 1101 66-67 1.37769884005737 1101 68-69 1.4448793495193648 1101 70-71 1.3864800645917477 1101 72-73 1.3681766183648598 1101 74-75 1.5172462449959632 1101 76-77 1.447363565541469 1101 78-79 1.5058265462579037 1101 80-81 1.6779832255608689 1101 82-83 1.3407012051823486 1101 84-85 1.3144991412083264 1101 86-87 1.3210312140724874 1101 88-89 1.4101283706512149 1101 90-91 1.4473153673702903 1101 92-93 1.6046733717230275 1101 94-95 1.7611625440862184 1101 96-97 1.757016322207516 1101 98-99 1.9418144592163955 1101 100-101 2.3631352802861123 1102 1 0.2877514232673093 1102 2 0.20806511183267418 1102 3 0.1750083266361031 1102 4 0.1701657351111976 1102 5 0.14119694681681239 1102 6 0.16358166342880054 1102 7 0.12170504422736173 1102 8 0.18532586080780078 1102 9 0.249447656871439 1102 10-11 0.31984559618717867 1102 12-13 0.269566234758301 1102 14-15 0.24504018057553623 1102 16-17 0.21932020458915247 1102 18-19 0.26168549062536073 1102 20-21 0.2616240495622648 1102 22-23 0.1273376074062753 1102 24-25 0.2454773579433649 1102 26-27 0.281193524712414 1102 28-29 0.3785946580755919 1102 30-31 0.4302537280821923 1102 32-33 0.33925922573048695 1102 34-35 0.37107214261094157 1102 36-37 0.5649560379488108 1102 38-39 0.6153146353188035 1102 40-41 0.6978214406160461 1102 42-43 0.9263837181603094 1102 44-45 0.8972217459380829 1102 46-47 0.7557040525295449 1102 48-49 0.8289110985021821 1102 50-51 1.0004121512480637 1102 52-53 0.9450437645797081 1102 54-55 0.8495992626712621 1102 56-57 0.878011463186624 1102 58-59 1.0779145198148896 1102 60-61 1.0260296525285852 1102 62-63 0.9659105152179208 1102 64-65 1.1056162918595618 1102 66-67 1.07141270288664 1102 68-69 0.9571922618297926 1102 70-71 0.9106691730422334 1102 72-73 0.9295482336995491 1102 74-75 0.976368783551866 1102 76-77 0.8975041012190452 1102 78-79 0.9792567712483731 1102 80-81 0.9796202557805209 1102 82-83 1.0434968785876428 1102 84-85 1.2524107042290709 1102 86-87 1.1689003657208552 1102 88-89 1.2634687779126175 1102 90-91 1.183516953162826 1102 92-93 1.2817138297161783 1102 94-95 1.4644142512807896 1102 96-97 1.4372352184263697 1102 98-99 1.5766415504291373 1102 100-101 1.8573121680429274 1103 1 0.16604475467106283 1103 2 0.19556816723944337 1103 3 0.17214837765808255 1103 4 0.10130502900999971 1103 5 0.09012113478455319 1103 6 0.1768635276813768 1103 7 0.18553502859956694 1103 8 0.12070125006882648 1103 9 0.14998743804695636 1103 10-11 0.1824670072424155 1103 12-13 0.3379252805713122 1103 14-15 0.3027328785725203 1103 16-17 0.3621325642279558 1103 18-19 0.41032937535556613 1103 20-21 0.32090710332771977 1103 22-23 0.3796478037729685 1103 24-25 0.3435274569076583 1103 26-27 0.26397867909759043 1103 28-29 0.16697895015219189 1103 30-31 0.35395966955780267 1103 32-33 0.3903548923915636 1103 34-35 0.5323025383710487 1103 36-37 0.41276810197425107 1103 38-39 0.3099698574832743 1103 40-41 0.408059457906333 1103 42-43 0.5855882799598291 1103 44-45 0.4332843700846709 1103 46-47 0.5210916104719772 1103 48-49 0.6332812346696386 1103 50-51 0.714170614835119 1103 52-53 0.6116556471779546 1103 54-55 0.8234222582141157 1103 56-57 0.7334984442858996 1103 58-59 0.7982799420522468 1103 60-61 0.9489506743448572 1103 62-63 0.7767552458435603 1103 64-65 0.9104168322584414 1103 66-67 0.8788181705866904 1103 68-69 0.9373947702194805 1103 70-71 0.8731024504590472 1103 72-73 0.8074041014483058 1103 74-75 0.933575881449304 1103 76-77 0.746532812777172 1103 78-79 0.8810048168910747 1103 80-81 0.8797038217071567 1103 82-83 1.0087157604278936 1103 84-85 1.0372977137425252 1103 86-87 1.213970814356049 1103 88-89 1.159802025062156 1103 90-91 1.2404496600899755 1103 92-93 1.3965677834787193 1103 94-95 1.448506281456293 1103 96-97 1.375880444447052 1103 98-99 1.5657261373249014 1103 100-101 2.2660327181132125 1104 1 0.31047215729786615 1104 2 0.2620910050716603 1104 3 0.1978767998680908 1104 4 0.1580122418566603 1104 5 0.1711491259700324 1104 6 0.1827255536352439 1104 7 0.23579858646910878 1104 8 0.1669673210931677 1104 9 0.1882058700046798 1104 10-11 0.19625009056920817 1104 12-13 0.2231683497616075 1104 14-15 0.26224013392105405 1104 16-17 0.25954803398925463 1104 18-19 0.2937021307248884 1104 20-21 0.1836682935652263 1104 22-23 0.19443453209815686 1104 24-25 0.0037138184231295668 1104 26-27 0.08054424980085884 1104 28-29 0.03040446329312374 1104 30-31 0.1318399805607129 1104 32-33 0.11770485381648399 1104 34-35 0.13815740368166018 1104 36-37 0.022512120527252932 1104 38-39 0.09535429163076259 1104 40-41 -0.003484884954509937 1104 42-43 0.17986530907823806 1104 44-45 0.16947010331143986 1104 46-47 0.10216025563207154 1104 48-49 0.08645940533317287 1104 50-51 0.23899541006381497 1104 52-53 0.29315231401401576 1104 54-55 0.17626618852951026 1104 56-57 0.23368970275740253 1104 58-59 0.1300392428038819 1104 60-61 0.14925581014298928 1104 62-63 0.2422605793678443 1104 64-65 0.2697778718914421 1104 66-67 0.19446001830983306 1104 68-69 0.23407107680587913 1104 70-71 0.4818250378997426 1104 72-73 0.4457839943620421 1104 74-75 0.6522600786025983 1104 76-77 0.4928425406265333 1104 78-79 0.5761270329022743 1104 80-81 0.6043082539143434 1104 82-83 0.495287244436561 1104 84-85 0.49523563327537445 1104 86-87 0.4535627038881138 1104 88-89 0.568340283686112 1104 90-91 0.4367069535516137 1104 92-93 0.7176300071587356 1104 94-95 0.577080943866612 1104 96-97 0.8233555092393239 1104 98-99 0.8916409672480761 1104 100-101 1.3915410082901971 1105 1 0.19577169333266653 1105 2 0.11892409604764964 1105 3 0.12889089523675068 1105 4 0.12785122753447098 1105 5 0.1668527225194012 1105 6 0.1500090764441353 1105 7 0.1336702941685175 1105 8 0.10943009172903828 1105 9 0.13418681240091246 1105 10-11 0.14766605389554144 1105 12-13 0.11849845558397476 1105 14-15 0.24160792147993249 1105 16-17 0.408109137021782 1105 18-19 0.3575143571278119 1105 20-21 0.39237627270821207 1105 22-23 0.35309601391379886 1105 24-25 0.33897416565357474 1105 26-27 0.3033932128726562 1105 28-29 0.2920422780951526 1105 30-31 0.35946518942065353 1105 32-33 0.4259687771828382 1105 34-35 0.5011152889479291 1105 36-37 0.5266285831013136 1105 38-39 0.5604757517224428 1105 40-41 0.5735736613550699 1105 42-43 0.569300965093781 1105 44-45 0.7524319200590455 1105 46-47 0.9374024575640405 1105 48-49 0.9825894163967632 1105 50-51 0.9199035318490161 1105 52-53 1.0569322821085336 1105 54-55 0.9672326020860638 1105 56-57 0.9277919767161293 1105 58-59 0.9725509049384229 1105 60-61 1.0055148040365935 1105 62-63 0.991055303118273 1105 64-65 0.987518177387706 1105 66-67 1.0013265016753863 1105 68-69 1.083221398002859 1105 70-71 0.9730147394347846 1105 72-73 1.0290351278131489 1105 74-75 1.0611210326924727 1105 76-77 1.024758356522316 1105 78-79 1.003879258851562 1105 80-81 0.9672641230363404 1105 82-83 0.9532178739524078 1105 84-85 0.9300753624063915 1105 86-87 1.1225140820487347 1105 88-89 1.1283150314790618 1105 90-91 1.1812070379776536 1105 92-93 1.2967500609897833 1105 94-95 1.3649377068965762 1105 96-97 1.4552093286986398 1105 98-99 1.5751032918120202 1105 100-101 1.5624286410091948 1106 1 -0.4271259785591077 1106 2 -0.27261544364417745 1106 3 -0.33319467782665413 1106 4 -0.18526516538793203 1106 5 -0.2063042367422483 1106 6 -0.1702123864759244 1106 7 -0.26010930674181765 1106 8 -0.294838513523473 1106 9 -0.6260017478460043 1106 10-11 -0.5353073804316608 1106 12-13 -0.7584968650382677 1106 14-15 -0.5840093300569151 1106 16-17 -0.5680494815654953 1106 18-19 -0.5161132080005828 1106 20-21 -0.5522037839348002 1106 22-23 -0.5010876949486374 1106 24-25 -0.4843391607805714 1106 26-27 -0.5592171226312885 1106 28-29 -0.5589255293981026 1106 30-31 -0.6164521656040662 1106 32-33 -0.6180283299251172 1106 34-35 -0.9892645393746022 1106 36-37 -0.7203041055000909 1106 38-39 -0.5902447180432588 1106 40-41 -0.7273328084253947 1106 42-43 -0.9113746750635983 1106 44-45 -0.7007249143132341 1106 46-47 -0.6797789709319702 1106 48-49 -0.6054777445668975 1106 50-51 -0.8489855601427934 1106 52-53 -1.1741062468308883 1106 54-55 -1.1065724762564741 1106 56-57 -1.2303551531841492 1106 58-59 -1.330518705560003 1106 60-61 -1.598251518862611 1106 62-63 -1.3853005271434427 1106 64-65 -1.953309237419294 1106 66-67 -2.166520274958266 1106 68-69 -2.0477974892441715 1106 70-71 -1.943227350668355 1106 72-73 -1.8929271794385478 1106 74-75 -2.0031774097667316 1106 76-77 -2.014584634544118 1106 78-79 -1.8362396358320687 1106 80-81 -1.9471247790668578 1106 82-83 -2.095681424907294 1106 84-85 -1.9857046370145781 1106 86-87 -1.8428651644028484 1106 88-89 -1.9476168561273113 1106 90-91 -2.1477070091817083 1106 92-93 -1.985665685516576 1106 94-95 -2.025891382563387 1106 96-97 -2.3603316211077505 1106 98-99 -2.2957043280292666 1106 100-101 -1.843229358524173 1107 1 0.13741357202133742 1107 2 0.14907436338460656 1107 3 0.023685682268130392 1107 4 0.0674445627999134 1107 5 0.11301154323949447 1107 6 0.14722753584384662 1107 7 0.1209562627831744 1107 8 0.14143880139159393 1107 9 0.13940059741290156 1107 10-11 0.20218424966118675 1107 12-13 0.15505268075581569 1107 14-15 0.3305137147234163 1107 16-17 0.17938369490552475 1107 18-19 0.1615784386916772 1107 20-21 0.19459911434640986 1107 22-23 0.07986592219293698 1107 24-25 0.11575057585675808 1107 26-27 0.17593654980002782 1107 28-29 0.20893427676328002 1107 30-31 0.14059992969649215 1107 32-33 0.25817598504673356 1107 34-35 0.22007705705141234 1107 36-37 0.1358891889640006 1107 38-39 0.28332584534431504 1107 40-41 0.39484568261373454 1107 42-43 0.3917931904837104 1107 44-45 0.459898986210149 1107 46-47 0.4074973973177336 1107 48-49 0.5412872122153587 1107 50-51 0.5185059389021163 1107 52-53 0.6246986583559249 1107 54-55 0.6608470712287513 1107 56-57 0.7034263225030273 1107 58-59 0.6646961724998874 1107 60-61 0.5114805380221767 1107 62-63 0.8266464271464073 1107 64-65 0.7853643202889131 1107 66-67 0.560110601059769 1107 68-69 0.6798848904865871 1107 70-71 0.6739685104632578 1107 72-73 0.6024820863996858 1107 74-75 0.7818721419557058 1107 76-77 0.5465610863974035 1107 78-79 0.750038895956731 1107 80-81 0.8217525207464931 1107 82-83 0.9162773236972015 1107 84-85 0.9014811833486505 1107 86-87 1.0204402890011828 1107 88-89 1.0280378687437732 1107 90-91 0.981130589346062 1107 92-93 0.830874374278725 1107 94-95 1.1033970832833369 1107 96-97 1.2340275763047401 1107 98-99 1.275694646177378 1107 100-101 1.7439699410557878 1108 1 0.05898208202469135 1108 2 0.022510601984798484 1108 3 -0.04128998904198511 1108 4 0.04007237410964848 1108 5 -0.0030859898160500165 1108 6 0.0476399688659086 1108 7 0.061930488547822904 1108 8 0.07973557347260396 1108 9 0.056189889195451315 1108 10-11 0.074715691273326 1108 12-13 0.17477951791964585 1108 14-15 0.12070297336224911 1108 16-17 0.10193054368962606 1108 18-19 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2316 74-75 -0.13664639146399438 2316 76-77 0.033799922692569595 2316 78-79 -0.150859108879299 2316 80-81 -0.2310032517106677 2316 82-83 -0.5044693238721116 2316 84-85 -0.3951227329502167 2316 86-87 -0.18506642793014905 2316 88-89 0.05116270026728742 2316 90-91 0.005542480668164984 2316 92-93 0.1535083608649792 2316 94-95 -0.03870212372045856 2316 96-97 0.3072455476667528 2316 98-99 0.013461140780094638 2316 100-101 0.4209927653453711 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 11.0 5 30.0 6 47.0 7 124.0 8 386.0 9 720.0 10 1413.0 11 2558.0 12 4190.0 13 6559.0 14 8313.0 15 12214.0 16 17477.0 17 25570.0 18 22019.0 19 10934.0 20 7934.0 21 7359.0 22 7442.0 23 7641.0 24 8487.0 25 9399.0 26 11050.0 27 13405.0 28 16404.0 29 20051.0 30 25071.0 31 31484.0 32 40792.0 33 53937.0 34 74247.0 35 108036.0 36 168076.0 37 264182.0 38 222813.0 39 19634.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.59997820177672 19.896328300763425 15.472915707571955 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34.06239126122347 19.233796093046244 20-21 21.08807201239339 25.53587957175975 34.107798266762764 19.268250149084093 22-23 20.358807503469535 25.81791248879057 34.04285072004592 19.780429287693977 24-25 20.089478801832826 25.90873097173036 34.629743449657404 19.372046776779413 26-27 20.039276438730262 25.828465783929182 34.839892211338295 19.29236556600226 28-29 19.462841161310255 26.508792754412635 34.7985349474386 19.22983113683851 30-31 19.807449033017562 26.22429699872847 34.49283421625155 19.47541975200242 32-33 19.54001261776308 26.35157217978361 34.40905454580931 19.699360656644 34-35 20.084031700503736 26.072509861692406 34.3565753830044 19.486883054799463 36-37 20.188624942784877 25.904974524535223 34.38598316920148 19.52041736347842 38-39 19.890131153192 26.45018910384614 34.33726662829306 19.322413114668798 40-41 19.984585497440875 26.53623688578268 34.04101021901846 19.438167397757987 42-43 19.682345684885195 26.78923484943662 33.47535239470646 20.05306707097172 44-45 20.088665801634455 26.690024162365894 32.981100997388644 20.240209038611006 46-47 20.558457966263745 27.212336139810017 32.011842160889486 20.21736373303675 48-49 20.572848069774928 27.4386753950368 31.512619389079134 20.475857146109146 50-51 20.72804980764415 27.885622254091828 31.205102064044905 20.181225874219113 52-53 20.652034289096367 28.491876502017867 30.43035352500626 20.425735683879505 54-55 20.355045316631056 28.135904365160663 30.617140320582237 20.89190999762604 56-57 20.86069079000855 28.3343983635932 30.064950585847942 20.7399602605503 58-59 21.042071134265356 27.823183838856856 30.394703466307643 20.74004156057014 60-61 20.782724070984674 27.949524069683875 30.9341290979275 20.333622761403955 62-63 20.833617883402763 27.890703505331654 30.941852599812037 20.333826011453546 64-65 20.57288871978485 28.117774460736968 30.88412958572762 20.425207233750562 66-67 20.64154658653737 28.12021346133208 30.317590397492054 20.92064955463849 68-69 20.702765501474783 28.089116203744354 30.440922527585094 20.767195767195766 70-71 20.903170050373493 28.13753036555741 30.168811361189974 20.790488222879127 72-73 20.785935421768244 27.847411244768345 30.312143296162965 21.05451003730045 74-75 20.548539363843606 28.72175230810756 29.718653151351372 21.011055176697464 76-77 20.464759693401366 28.201676081208966 30.192103816873335 21.14146040851634 78-79 20.458824791953248 28.183871376864616 30.0760073885458 21.281296442636332 80-81 20.468824694393227 28.491591951948436 29.844749482118875 21.194833871539466 82-83 20.50790602685667 28.32637269097593 29.997861807739927 21.167859474427463 84-85 20.379720644434045 28.147790728445592 29.788225212894698 21.68426341422566 86-87 20.207079280527346 28.17765192534707 29.819871676048688 21.7953971180769 88-89 20.295005251981284 27.862492398448147 29.857513585233313 21.984988764337256 90-91 20.134540323272418 27.653049069775665 30.121722119390203 22.090688487561707 92-93 20.083423815503366 27.866692629053457 30.174607891400257 21.87527566404292 94-95 19.756839770668904 27.906841559269342 30.205680920186147 22.13063774987561 96-97 19.612044435338845 27.89875220729554 30.231778226553885 22.257425130811733 98-99 19.70435940608468 27.938214985768372 29.77743216095518 22.579993447191764 100-101 19.403185315431603 27.745296730325546 30.265700433991604 22.585817520251247 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 715.0 1 541.5 2 555.0 3 727.5 4 591.5 5 545.5 6 1012.5 7 2132.0 8 3379.0 9 3802.5 10 3466.0 11 3254.0 12 3360.5 13 3576.0 14 3835.5 15 4142.0 16 4288.5 17 4415.0 18 4685.0 19 5018.0 20 5416.5 21 5965.5 22 6598.0 23 7383.0 24 8365.0 25 9557.0 26 11287.0 27 13451.5 28 15068.5 29 16810.5 30 18948.0 31 21021.0 32 23816.0 33 26619.0 34 29655.5 35 32686.5 36 36304.0 37 39531.0 38 40078.5 39 39757.0 40 39773.5 41 39892.5 42 39800.0 43 40781.0 44 42863.0 45 43422.0 46 43037.5 47 46002.0 48 55665.5 49 54150.5 50 42593.0 51 36489.5 52 32422.0 53 30188.0 54 28210.0 55 25333.5 56 22082.0 57 19583.5 58 17588.0 59 15622.5 60 13085.5 61 10583.5 62 8418.5 63 5812.5 64 3736.0 65 2649.0 66 1920.5 67 1483.5 68 1126.5 69 790.0 70 599.0 71 483.5 72 397.5 73 377.0 74 310.0 75 237.5 76 188.0 77 110.0 78 71.5 79 46.5 80 31.5 81 18.0 82 11.5 83 10.5 84 6.0 85 3.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04504021098981148 2 0.0 3 0.0 4 8.130001983720483E-5 5 0.00756090184486005 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0053251512993369166 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.2195002975580726E-4 22-23 0.0017073004165813017 24-25 0.0 26-27 0.0010162502479650604 28-29 0.0017886004364185064 30-31 0.0 32-33 0.0 34-35 0.0 36-37 4.0650009918602426E-4 38-39 0.0 40-41 1.2195002975580726E-4 42-43 0.020121754909708196 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.130001983720483E-5 84-85 2.0325004959301213E-4 86-87 0.0 88-89 0.0 90-91 0.009796652390383184 92-93 0.002235750545523133 94-95 0.0 96-97 0.0 98-99 4.0650009918602426E-4 100-101 0.012154352965662123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1230012.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.56995501180339 #Duplication Level Percentage of deduplicated Percentage of total 1 77.84408405117517 43.257922486588456 2 12.999299918874478 14.447410233535882 3 4.085929948945676 6.811648303328741 4 1.7395987875747123 3.866777054564579 5 0.879454082993887 2.4435611913458555 6 0.5429755086390643 1.810387475255032 7 0.3439428511352897 1.3379022134953606 8 0.2515022967375271 1.1180777052055686 9 0.18137877832652335 0.9071289496530688 >10 1.0025897791753562 10.332505399993469 >50 0.08227556756154016 3.1359393217200533 >100 0.04095060777880422 4.23241190589165 >500 0.0030822986029825435 1.2075465226398248 >1k 0.002495194107176345 2.386545846162869 >5k 1.467761239515497E-4 0.6433723046661897 >10k+ 2.935522479030994E-4 2.0608630859534247 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15101 1.2277115995616303 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10166 0.8264960016650243 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7888 0.6412945564758717 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4445 0.3613785881763755 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2691 0.21877835338191826 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2314 0.188128245903292 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2109 0.171461741836665 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2031 0.16512034028936304 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2021 0.164307340090991 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1966 0.15983583899994472 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1491 0.12121832957727241 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1338 0.10877942654218009 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1290 0.10487702558999423 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.130001983720483E-5 0.0 16-17 0.0 0.0 0.0 1.2195002975580726E-4 0.0 18-19 0.0 0.0 0.0 1.6260003967440967E-4 0.0 20-21 0.0 0.0 0.0 2.0325004959301208E-4 0.0 22-23 4.065000991860242E-5 0.0 0.0 3.6585008926742177E-4 0.0 24-25 8.130001983720483E-5 0.0 0.0 6.097501487790362E-4 0.0 26-27 8.130001983720483E-5 0.0 0.0 8.943002182092532E-4 0.0 28-29 8.130001983720483E-5 0.0 0.0 0.0026422506447091572 0.0 30-31 8.130001983720483E-5 0.0 0.0 0.005772301408441543 0.0 32-33 8.130001983720483E-5 0.0 0.0 0.009959252430057592 0.0 34-35 8.130001983720483E-5 0.0 0.0 0.014105553441755039 0.0 36-37 8.130001983720483E-5 0.0 0.0 0.020040454889870996 0.0 38-39 8.130001983720483E-5 0.0 0.0 0.029918407300091383 0.0 40-41 8.130001983720483E-5 0.0 0.0 0.04243861035502093 0.0 42-43 8.130001983720483E-5 0.0 0.0 0.05951161452083394 0.0 44-45 8.130001983720483E-5 0.0 0.0 0.07455211819071683 0.0 46-47 8.130001983720483E-5 0.0 0.0 0.10475507556023844 0.0 48-49 8.130001983720483E-5 0.0 0.0 0.14040513425885276 0.0 50-51 8.130001983720483E-5 0.0 0.0 0.16016103907929352 0.0 52-53 8.130001983720483E-5 0.0 0.0 0.19394119732165216 0.0 54-55 8.130001983720483E-5 0.0 0.0 0.24077000874788212 0.0 56-57 8.130001983720483E-5 0.0 0.0 0.32129767839663353 0.0 58-59 8.130001983720483E-5 0.0 0.0 0.4262966540163836 0.0 60-61 8.130001983720483E-5 0.0 0.0 0.551905184664865 0.0 62-63 8.130001983720483E-5 0.0 0.0 0.6851965671879624 0.0 64-65 8.130001983720483E-5 0.0 0.0 0.8316179029147683 0.0 66-67 8.130001983720483E-5 0.0 0.0 1.020721749056107 0.0 68-69 8.130001983720483E-5 0.0 0.0 1.257182856752617 0.0 70-71 8.130001983720483E-5 0.0 0.0 1.405026942826574 0.0 72-73 8.130001983720483E-5 0.0 0.0 1.5757569844847041 0.0 74-75 8.130001983720483E-5 0.0 0.0 1.7787631340182046 0.0 76-77 8.130001983720483E-5 0.0 0.0 2.0005495881340996 0.0 78-79 8.130001983720483E-5 0.0 0.0 2.2333521949379356 0.0 80-81 8.130001983720483E-5 0.0 0.0 2.55753602403879 0.0 82-83 8.130001983720483E-5 0.0 0.0 2.9115163104099797 0.0 84-85 8.130001983720483E-5 0.0 0.0 3.3657395212404433 0.0 86-87 8.130001983720483E-5 0.0 0.0 3.9696360685912007 0.0 88-89 8.130001983720483E-5 0.0 0.0 4.61971102720949 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAAC 40 4.496783E-4 29.685617 80-81 GTATCAA 18960 0.0 25.696236 1 GTACATG 10355 0.0 24.465895 1 TACATGG 10430 0.0 23.820677 2 AGTACTT 11870 0.0 23.490314 12-13 ACATGGG 10420 0.0 23.06851 3 GAGTACT 11500 0.0 22.841715 12-13 GTACGTG 130 2.9283992E-4 21.93774 1 TATCAAC 22335 0.0 21.82218 2 GTACTTT 12070 0.0 21.741581 14-15 ATCAACG 22420 0.0 21.718258 3 TCAACGC 22585 0.0 21.55959 4 CAACGCA 22840 0.0 21.360483 5 CATGGGA 7875 0.0 21.354502 4 AACGCAG 23185 0.0 21.121155 6 TACTTTT 13145 0.0 21.101746 14-15 GTATAGG 390 0.0 20.718977 1 TACACCG 115 0.003721283 20.654224 5 GGTATCA 13910 0.0 20.229195 1 ATGGGAG 2455 0.0 20.124197 5 >>END_MODULE