FastQCFastQC Report
Thu 26 May 2016
SRR937624_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937624_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010458
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97420.964117261677378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63810.6314958167484448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45590.45118154341892486No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37740.37349399975060815No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17800.1761577423307055No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16610.16438090450073137No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16570.16398504440560616No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15870.15705749274091552No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13750.13607690769928094No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13560.1341965722474363No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13310.13172244665290392No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12800.12667523044005785No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12680.12548765015468233No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10180.10074639420935853No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG650.005821223429.2538645
TACCTGG7800.026.1961842
TACATGG91600.025.7306272
GTACATG92350.025.6360231
ACATGGG92800.024.1689723
GTATCAA145050.023.8600751
CATGGGA67400.022.7802094
TGGGCGA1050.0021941922.622326
GAGTACT73200.022.32231112-13
AGTACTT74850.022.21099512-13
AGTATCG1302.9254932E-421.94045
ATGGGAG22000.021.8240475
ATGGGAT25300.021.7958645
GTACTTT76650.021.2550914-15
CATGGGG21200.021.0769164
TATCAAC168700.020.4214732
TCAACGC168600.020.3842984
ATCAACG168850.020.375193
CAACGCA169850.020.2072985
GTATAGG3302.7284841E-1120.168441
Produced by FastQC (version 0.11.5)