##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937624_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1010458 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96146005078885 33.0 31.0 34.0 30.0 34.0 2 32.03037038649801 33.0 31.0 34.0 30.0 34.0 3 32.072788774991146 34.0 31.0 34.0 30.0 34.0 4 35.68549113372352 37.0 35.0 37.0 35.0 37.0 5 35.70735943502847 37.0 35.0 37.0 35.0 37.0 6 35.799769015634496 37.0 35.0 37.0 35.0 37.0 7 35.736133515692885 37.0 35.0 37.0 35.0 37.0 8 35.74955614186834 37.0 35.0 37.0 35.0 37.0 9 37.31708096724456 39.0 37.0 39.0 35.0 39.0 10-11 37.26690025711113 39.0 37.0 39.0 34.0 39.0 12-13 37.180291016548935 39.0 37.0 39.0 34.0 39.0 14-15 38.28340465412714 40.0 38.0 41.0 33.5 41.0 16-17 38.24394235089434 40.0 38.0 41.0 34.0 41.0 18-19 38.26246860334621 40.0 38.0 41.0 34.0 41.0 20-21 38.31096393912463 40.0 38.0 41.0 34.0 41.0 22-23 38.30910982940409 40.0 38.0 41.0 34.0 41.0 24-25 38.236989068323474 40.0 38.0 41.0 34.0 41.0 26-27 38.116880167211306 40.0 38.0 41.0 34.0 41.0 28-29 37.97592131488889 40.0 38.0 41.0 33.5 41.0 30-31 37.70039328700451 40.0 37.0 41.0 33.0 41.0 32-33 37.496002802689475 40.0 37.0 41.0 33.0 41.0 34-35 37.44047402267091 40.0 36.5 41.0 33.0 41.0 36-37 37.262657626541625 40.0 36.0 41.0 33.0 41.0 38-39 37.06374930971896 40.0 36.0 41.0 32.0 41.0 40-41 36.80313679539377 39.5 35.5 41.0 31.0 41.0 42-43 36.46983447110122 39.0 35.0 41.0 30.5 41.0 44-45 36.073955077796406 39.0 35.0 41.0 30.0 41.0 46-47 35.50909389603527 39.0 35.0 41.0 27.0 41.0 48-49 35.00253696838463 39.0 35.0 40.0 24.0 41.0 50-51 34.06370230133267 38.0 34.0 39.5 22.5 40.5 52-53 34.13127660922077 38.0 34.5 39.5 23.0 40.5 54-55 34.400703443389034 39.0 35.0 40.0 22.0 41.0 56-57 34.191298401319 38.0 34.0 40.0 20.5 41.0 58-59 33.93993614776666 38.0 34.0 40.0 18.5 41.0 60-61 33.59347543391215 38.0 34.0 40.0 16.0 41.0 62-63 33.25778161981992 37.5 33.0 40.0 12.5 41.0 64-65 32.851582153835196 37.0 33.0 40.0 8.0 41.0 66-67 32.345037101987415 36.0 32.0 39.0 6.5 41.0 68-69 31.90736428431464 36.0 31.5 39.0 2.0 40.0 70-71 31.453388463449247 35.0 31.0 38.5 2.0 40.0 72-73 30.990317756898357 35.0 31.0 38.0 2.0 40.0 74-75 30.46870874395571 35.0 30.0 37.0 2.0 39.0 76-77 29.965344427972266 34.5 29.5 36.5 2.0 39.0 78-79 29.47522064252052 34.0 29.0 36.0 2.0 38.5 80-81 28.959814757268486 34.0 29.0 36.0 2.0 37.5 82-83 28.440632861534077 34.0 28.5 35.0 2.0 37.0 84-85 27.915031104706976 34.0 26.5 35.0 2.0 36.5 86-87 27.385296568486766 33.5 26.0 35.0 2.0 36.0 88-89 26.865612920081784 33.0 25.5 35.0 2.0 36.0 90-91 26.33259571402275 33.0 24.5 35.0 2.0 35.0 92-93 25.722172519788057 32.0 22.0 35.0 2.0 35.0 94-95 25.01541677140465 32.0 19.0 34.0 2.0 35.0 96-97 24.18905634870524 31.0 6.5 34.0 2.0 35.0 98-99 23.10969728578526 31.0 2.0 34.0 2.0 35.0 100-101 19.974928200875247 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11543748200688952 1101 2 0.053982197282014255 1101 3 0.13766752724230713 1101 4 0.073883948286948 1101 5 0.07571930957527684 1101 6 0.07806304903154881 1101 7 0.06283039250814682 1101 8 0.11649932808911956 1101 9 0.1387198928700215 1101 10-11 0.23510498969304905 1101 12-13 0.18430028123283648 1101 14-15 0.3429238448696452 1101 16-17 0.3488863014088892 1101 18-19 0.4185070475934438 1101 20-21 0.3230167166312796 1101 22-23 0.20038679886856414 1101 24-25 0.19282734732578888 1101 26-27 0.26882700283051264 1101 28-29 0.08649409694348975 1101 30-31 0.2079482828063135 1101 32-33 0.22813248358345106 1101 34-35 0.22457515007031503 1101 36-37 0.28466755735222904 1101 38-39 0.24232659315291016 1101 40-41 0.389252168789362 1101 42-43 0.460246635347751 1101 44-45 0.7374948114768216 1101 46-47 0.6643664800285976 1101 48-49 0.8246761773504474 1101 50-51 0.5073035850374126 1101 52-53 0.48949811240812124 1101 54-55 0.7126865612164508 1101 56-57 0.6930624075715954 1101 58-59 0.6380636963795183 1101 60-61 0.6967044191000653 1101 62-63 0.6954672918162288 1101 64-65 0.8130358621468474 1101 66-67 0.8971131085788073 1101 68-69 0.9941388619012734 1101 70-71 0.8985272707416065 1101 72-73 1.0352457730509421 1101 74-75 1.013547674555337 1101 76-77 1.048657040603537 1101 78-79 1.0887471861546203 1101 80-81 1.1903324405047506 1101 82-83 1.2369316072224272 1101 84-85 1.290655558756928 1101 86-87 1.5547277392926304 1101 88-89 1.8030353643406194 1101 90-91 1.8528156623446534 1101 92-93 1.5769641144594964 1101 94-95 1.7774970980951217 1101 96-97 2.0077052026183644 1101 98-99 2.3584288520013743 1101 100-101 3.0781759925377443 1102 1 0.14793165213767878 1102 2 0.10717240310173892 1102 3 -0.02328451574663859 1102 4 0.0565773478611149 1102 5 -0.10146410317946675 1102 6 -0.016325068174168678 1102 7 0.11647076561978054 1102 8 0.06211577338973484 1102 9 0.13809584339217906 1102 10-11 0.17722275305105484 1102 12-13 0.05662944071778497 1102 14-15 0.21187497535731836 1102 16-17 0.22298469946222355 1102 18-19 0.07030264730955338 1102 20-21 0.21140412411379117 1102 22-23 0.15614442839305553 1102 24-25 0.07453904761981534 1102 26-27 0.1751440463264018 1102 28-29 0.35826941885365926 1102 30-31 0.31655190239467146 1102 32-33 0.3082290566342465 1102 34-35 0.43772530722331027 1102 36-37 0.3416301954947869 1102 38-39 0.4991665505695124 1102 40-41 0.42084828558952125 1102 42-43 0.34797983011607414 1102 44-45 0.5812439243228127 1102 46-47 0.596795761180374 1102 48-49 0.663256477461978 1102 50-51 0.7232064543469505 1102 52-53 0.7378061829748859 1102 54-55 0.843126082660234 1102 56-57 0.8002219939043513 1102 58-59 0.9653905746112912 1102 60-61 1.0000451876289134 1102 62-63 0.9083216464750592 1102 64-65 0.9225225801839585 1102 66-67 0.7627029392591282 1102 68-69 0.7972809079318957 1102 70-71 0.9366591778692488 1102 72-73 0.9938154273143489 1102 74-75 0.7946984400425094 1102 76-77 0.8933711235397652 1102 78-79 1.0940921623281987 1102 80-81 1.1323374085672064 1102 82-83 1.3533660085202044 1102 84-85 1.4033256388542625 1102 86-87 1.3588943214684406 1102 88-89 1.1912841223250048 1102 90-91 1.2252804296568307 1102 92-93 1.3463981102011573 1102 94-95 1.6570532676175809 1102 96-97 1.6745403440560587 1102 98-99 1.6959217547692944 1102 100-101 2.5003629539085246 1103 1 0.1667829470416038 1103 2 0.10012561947601384 1103 3 0.15126017932436753 1103 4 0.09277617827046925 1103 5 0.20118000709622663 1103 6 0.15247192430702938 1103 7 0.23777820087457968 1103 8 0.09663122869466889 1103 9 0.20216852509643957 1103 10-11 0.1977072977461276 1103 12-13 0.22257137889656065 1103 14-15 0.5323971140373516 1103 16-17 0.42042204282061135 1103 18-19 0.4250102496487358 1103 20-21 0.3933088860185521 1103 22-23 0.27697776172638555 1103 24-25 0.29790788638339194 1103 26-27 0.43639509060376014 1103 28-29 0.4477598115019461 1103 30-31 0.3546220778332696 1103 32-33 0.4283877867929746 1103 34-35 0.5821384233469544 1103 36-37 0.7904063024287851 1103 38-39 0.847558363435013 1103 40-41 0.8185989121559345 1103 42-43 0.911179495947323 1103 44-45 0.9200158541473726 1103 46-47 1.0288028622664243 1103 48-49 1.2494657506449371 1103 50-51 1.2184654351322521 1103 52-53 1.149616959917239 1103 54-55 1.1238487225934009 1103 56-57 1.2233548259845506 1103 58-59 1.241382638103353 1103 60-61 1.5290029988235574 1103 62-63 1.5372752805602659 1103 64-65 1.4882744598832076 1103 66-67 1.568154067078673 1103 68-69 1.5448498302376557 1103 70-71 1.654397273107353 1103 72-73 1.4426855192107517 1103 74-75 1.5966011133925484 1103 76-77 1.5288055429215532 1103 78-79 1.680917559153599 1103 80-81 2.02135787640346 1103 82-83 2.002837604176012 1103 84-85 2.1709969003413114 1103 86-87 2.123332500248722 1103 88-89 2.2172636544887503 1103 90-91 2.147038992731183 1103 92-93 2.262596940610514 1103 94-95 2.45033856419235 1103 96-97 2.4689492830702626 1103 98-99 2.905585497459356 1103 100-101 3.5515784927556417 1104 1 0.16978660040813764 1104 2 0.03435967449896182 1104 3 0.04899158415930316 1104 4 -0.00395301058804165 1104 5 -0.0029919535295519495 1104 6 0.1187830935982177 1104 7 0.1902020855069111 1104 8 0.1952806809035863 1104 9 0.4410287517420599 1104 10-11 0.26680656536164093 1104 12-13 0.2276193560694395 1104 14-15 0.27219528050937924 1104 16-17 0.29507713336335684 1104 18-19 0.3044357855685149 1104 20-21 0.3071408782155842 1104 22-23 0.12561224112165092 1104 24-25 0.11322464695098944 1104 26-27 0.08236371400327158 1104 28-29 0.07059463569972735 1104 30-31 0.024732224267388858 1104 32-33 0.1682429034311923 1104 34-35 0.2752318387313082 1104 36-37 0.11701764454902985 1104 38-39 0.17333443645100743 1104 40-41 0.32994563118467113 1104 42-43 0.23873252109736853 1104 44-45 0.3716042023037929 1104 46-47 0.44164370767425964 1104 48-49 0.6408609815898956 1104 50-51 0.5506335627177279 1104 52-53 0.5421287349656865 1104 54-55 0.77623560298629 1104 56-57 0.6583947392277949 1104 58-59 0.7175825486942671 1104 60-61 0.8105739785161461 1104 62-63 0.7692164630703076 1104 64-65 0.8819067896102268 1104 66-67 0.9633312703282044 1104 68-69 1.1137643440276186 1104 70-71 1.0858926400910072 1104 72-73 1.006829587623681 1104 74-75 0.8985891287247121 1104 76-77 0.9335378076237859 1104 78-79 1.2518199813270492 1104 80-81 1.3595100493865182 1104 82-83 1.4389833666558545 1104 84-85 1.4960526740721534 1104 86-87 1.6008542963874497 1104 88-89 1.6853020447829437 1104 90-91 1.8125351062165542 1104 92-93 2.056824225782723 1104 94-95 2.071157405981044 1104 96-97 2.399675154381068 1104 98-99 2.5732205843230638 1104 100-101 3.3347876586843626 1105 1 0.19795693658774383 1105 2 0.2552697482344861 1105 3 0.12499614164905637 1105 4 -0.022670439749504112 1105 5 0.021064341826857458 1105 6 -2.0623125887198057E-4 1105 7 0.1451243686539101 1105 8 0.1472548378650842 1105 9 0.2775838332783991 1105 10-11 0.2412208567172982 1105 12-13 0.20163260127404214 1105 14-15 0.3269761131575706 1105 16-17 0.2806300850500847 1105 18-19 0.17906370925645376 1105 20-21 0.2115242252515941 1105 22-23 0.2263877912249086 1105 24-25 0.3152059249902308 1105 26-27 0.28932230169935025 1105 28-29 0.34405955791813625 1105 30-31 0.2251138492973297 1105 32-33 0.23139909456092056 1105 34-35 0.2783068495747614 1105 36-37 0.24102020813700875 1105 38-39 0.12715327623322992 1105 40-41 0.33108532730886253 1105 42-43 0.33601712467991973 1105 44-45 0.5890094110484725 1105 46-47 0.5364291366544549 1105 48-49 0.5152823940232949 1105 50-51 0.4269359107312667 1105 52-53 0.497967371344032 1105 54-55 0.5516911901191435 1105 56-57 0.5414925375200355 1105 58-59 0.47123760368840806 1105 60-61 0.5214737590574785 1105 62-63 0.7851832141109583 1105 64-65 0.8800478646340153 1105 66-67 0.8281738621415542 1105 68-69 0.8696829306878975 1105 70-71 0.6577527303344901 1105 72-73 0.5980094854685838 1105 74-75 0.7188071631777824 1105 76-77 0.894755447180728 1105 78-79 1.2281029082194763 1105 80-81 1.215567497062782 1105 82-83 1.2694564004291848 1105 84-85 1.1458604049730994 1105 86-87 1.312984081266162 1105 88-89 1.1463947417940332 1105 90-91 0.9943828316795873 1105 92-93 1.1251370482542562 1105 94-95 1.1555836089576523 1105 96-97 1.0277451481015731 1105 98-99 1.1696606928223936 1105 100-101 1.954206190318132 1106 1 -0.2068613630662952 1106 2 -0.237026753905635 1106 3 -0.26042586754552843 1106 4 -0.18316448564784338 1106 5 -0.18468553927760922 1106 6 -0.24168258736730763 1106 7 -0.2103928502164436 1106 8 -0.0796146195050369 1106 9 0.020596972400156233 1106 10-11 -0.3616237771310935 1106 12-13 -0.5821230054661726 1106 14-15 -0.743245452160366 1106 16-17 -0.6040034835876114 1106 18-19 -0.3134486698664176 1106 20-21 -0.3239812439428036 1106 22-23 -0.3685262776132774 1106 24-25 -0.39131561141977755 1106 26-27 -0.4098785338331652 1106 28-29 -0.3120181018448207 1106 30-31 -0.19753924618277807 1106 32-33 -0.03522156808350019 1106 34-35 0.10025729578268994 1106 36-37 -0.24367350765138696 1106 38-39 -0.34028286987305734 1106 40-41 -0.10385177462423201 1106 42-43 0.08191592826450034 1106 44-45 0.11981873991817338 1106 46-47 -0.17131352679192702 1106 48-49 -0.010681126030412713 1106 50-51 0.2363104736153545 1106 52-53 0.19719012095471555 1106 54-55 0.3367998746216898 1106 56-57 0.11072703680665796 1106 58-59 -0.10745678351004528 1106 60-61 0.1362261299742329 1106 62-63 0.00897910545442926 1106 64-65 0.2474634954330881 1106 66-67 0.5447949910429557 1106 68-69 0.38981590717921577 1106 70-71 0.41034651792645604 1106 72-73 0.2772382031686327 1106 74-75 0.3754014356770128 1106 76-77 0.7076395263698103 1106 78-79 0.7214792615303658 1106 80-81 0.7636493158380127 1106 82-83 0.6087562689447168 1106 84-85 0.418805843581044 1106 86-87 0.6097305400443851 1106 88-89 0.7501961349698938 1106 90-91 1.1451479593873763 1106 92-93 1.3499992693160117 1106 94-95 1.1208027031135899 1106 96-97 0.9922891021472857 1106 98-99 0.9407294470769898 1106 100-101 1.193900512240603 1107 1 -0.06666218539733748 1107 2 0.09258634645685859 1107 3 -0.04974887926982774 1107 4 0.04162092084586533 1107 5 0.026472404757043932 1107 6 0.08092049671844848 1107 7 0.004175403030295399 1107 8 0.05764487391883932 1107 9 0.35561996415159314 1107 10-11 0.1491391862910163 1107 12-13 0.03277089730041638 1107 14-15 0.0861466840407985 1107 16-17 0.0011180639191152864 1107 18-19 0.1816751963291594 1107 20-21 0.018308886018552073 1107 22-23 0.10377514734729942 1107 24-25 0.17656285714362951 1107 26-27 0.27800585771762343 1107 28-29 0.1986771374966736 1107 30-31 0.19204754509619448 1107 32-33 0.15210783036595643 1107 34-35 0.13596682605306398 1107 36-37 0.190152604458369 1107 38-39 0.26438986211635296 1107 40-41 0.2509891197009466 1107 42-43 0.2992442247628233 1107 44-45 0.2920990441485216 1107 46-47 0.4253671897518032 1107 48-49 0.28353581141778506 1107 50-51 0.28515790159251253 1107 52-53 0.45312286520333345 1107 54-55 0.5800464063452608 1107 56-57 0.4589786076609741 1107 58-59 0.009220328735160876 1107 60-61 0.1149378113450581 1107 62-63 0.5341363056720638 1107 64-65 0.4517032524528659 1107 66-67 0.8031830201803842 1107 68-69 0.6290144902537129 1107 70-71 0.24576437550386387 1107 72-73 0.32201415124836785 1107 74-75 0.4494634571604692 1107 76-77 0.43899359475047106 1107 78-79 0.6391147269095896 1107 80-81 0.5919561449506467 1107 82-83 0.7175062201604163 1107 84-85 0.9330439706314202 1107 86-87 0.8624750871080273 1107 88-89 0.7541000264644389 1107 90-91 0.8920653658535294 1107 92-93 1.0686039925540989 1107 94-95 1.2071038434034485 1107 96-97 1.3812801510899853 1107 98-99 1.6525611883204974 1107 100-101 2.3937554220068726 1108 1 0.06927364347966858 1108 2 0.07489967582901258 1108 3 0.014247951785826274 1108 4 0.17369855998232708 1108 5 0.12121987950452251 1108 6 0.19783077598167154 1108 7 0.20281275696176948 1108 8 0.1664664227403847 1108 9 0.320238700535036 1108 10-11 0.2893114976397939 1108 12-13 0.2722896138779589 1108 14-15 0.2780005164828623 1108 16-17 0.1523678163453397 1108 18-19 0.346060223067127 1108 20-21 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2316 48-49 -0.6963711008420645 2316 50-51 -0.40937566941163794 2316 52-53 -0.8102362158028029 2316 54-55 -0.8184285391885027 2316 56-57 -0.8238001604119134 2316 58-59 -0.9720234819204165 2316 60-61 -0.8715743692847653 2316 62-63 -0.6541286972988161 2316 64-65 -0.7493662662637135 2316 66-67 -0.8952325076469094 2316 68-69 -0.8270659217090532 2316 70-71 -0.7131383775165396 2316 72-73 -0.7668549317383615 2316 74-75 -1.0953473964739153 2316 76-77 -1.2375265082402223 2316 78-79 -1.1598058514227532 2316 80-81 -1.0481706128231707 2316 82-83 -1.1185801335729941 2316 84-85 -1.4155399073642236 2316 86-87 -1.5291767251320216 2316 88-89 -1.4559217600279375 2316 90-91 -1.5824048262637191 2316 92-93 -1.6300591090501797 2316 94-95 -1.5544727064083936 2316 96-97 -1.4616590447903839 2316 98-99 -1.3718991008456776 2316 100-101 -1.683051866565119 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3407.0 3 483.0 4 486.0 5 523.0 6 614.0 7 719.0 8 885.0 9 1196.0 10 1554.0 11 2255.0 12 3716.0 13 5547.0 14 6232.0 15 9028.0 16 12630.0 17 18043.0 18 14513.0 19 7702.0 20 7056.0 21 7126.0 22 7374.0 23 7977.0 24 8909.0 25 10318.0 26 11732.0 27 13816.0 28 16397.0 29 19575.0 30 24491.0 31 30571.0 32 39195.0 33 52007.0 34 70254.0 35 99962.0 36 143510.0 37 196394.0 38 141249.0 39 13011.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.542884430002864 19.6928948045076 14.452528946853747 20.311691818635786 2 14.639887272510766 21.678111417627562 41.61648837993927 22.065512929922402 3 20.182357307590017 27.808100094076366 29.42139066302005 22.58815193531357 4 11.938687113557046 16.80557361009537 38.49639146255665 32.75934781379093 5 12.45662414004908 39.74622951275153 32.851142284728226 14.946004062471161 6 29.15119234503283 37.235754781627364 18.321067919090908 15.291984954248896 7 27.103514542369755 35.49605490313515 20.35842521371547 17.04200534077962 8 26.229732543956207 33.975743511934866 19.946548458433742 19.847975485675185 9 27.95451863472605 17.632458129754376 21.067459463533293 33.34556377198628 10-11 24.328226772553098 28.44747395812673 26.330803192459957 20.893496076860217 12-13 26.418238823747004 26.056510879854255 27.56029749458152 19.96495280181722 14-15 23.24495052794161 26.385825034274678 28.150952377410693 22.218272060373017 16-17 20.140290546600646 30.262221752868307 28.314931850307172 21.28255585022388 18-19 19.92214534587416 28.1898396305176 32.51700963565371 19.37100538795454 20-21 21.74759293494616 26.60262368975397 32.18350459549756 19.46627877980232 22-23 20.83246362210721 26.940191467029724 32.183298272195735 20.044046638667336 24-25 20.48520112762757 27.055971854923555 32.7146354595836 19.744191557865282 26-27 20.419455874529156 26.978393545185043 32.95533482642 19.646815753865805 28-29 19.85077113480579 27.785000555344 32.811409145722266 19.552819164127953 30-31 20.14602919592209 27.47441389979769 32.59406362806934 19.78549327621088 32-33 19.839522554310637 27.535884787864724 32.524648788237606 20.099943869587033 34-35 20.41616771663109 27.367254497988085 32.53332738312173 19.683250402259098 36-37 20.41625165282516 27.196119387149537 32.562750340728634 19.82487861929667 38-39 20.135795283850417 27.744981295429106 32.564048158940764 19.555175261779713 40-41 20.257729020168696 27.91063014472781 32.178700052299305 19.652940782804183 42-43 19.834044932902074 28.076916212373305 31.746182051826132 20.34285680289849 44-45 20.258235386567257 27.948727737324347 31.36147450888623 20.43156236722216 46-47 20.638300087710732 28.37583428076458 30.61848212342273 20.367383508101963 48-49 20.65564162396578 28.5697426839923 30.288455340345966 20.486160351695954 50-51 20.681779980156772 28.938188990628266 30.142566797351655 20.237464231863306 52-53 20.606038667345686 29.4283502682932 29.530450943823798 20.43516012053731 54-55 20.386346000808192 29.02597869444603 29.67927629540198 20.9083990093438 56-57 20.83215810181066 29.32500484550642 29.162017376407366 20.680819676275558 58-59 21.050369938621117 28.890402214080662 29.398770439802703 20.66045740749552 60-61 20.82084136388512 28.95861588978514 29.98464280898787 20.235899937341866 62-63 20.737455333487652 28.865137244969024 30.03131178898521 20.366095632558114 64-65 20.467003687132117 29.18649115693065 29.863985572695196 20.48251958324204 66-67 20.682381832629666 29.072908316962337 29.382036368647597 20.862673481760403 68-69 20.468218903341864 29.1986455478408 29.582466477011636 20.750669071805696 70-71 20.687993670704106 29.126063433742168 29.360090735770733 20.825852159782997 72-73 20.581424460181992 28.934294772484463 29.531484322906724 20.95279644442682 74-75 20.2725984155936 29.82370640440687 29.106018592655648 20.797676587343886 76-77 20.38033913027096 29.350256464664593 29.220908637499864 21.04849576756458 78-79 20.390916878852057 29.17435095066987 29.251243366727742 21.18348880375033 80-81 20.457345352455654 29.438520979674475 29.042312327631635 21.061821340238232 82-83 20.52064883814192 29.190082529919515 29.161579494470164 21.127689137468405 84-85 20.376569141355937 29.059122396123772 29.045045045045043 21.519263417475244 86-87 20.41268031527289 29.147077777226986 29.023695037921975 21.41654686957815 88-89 20.525764471833657 28.73471192661824 29.063934399410662 21.675589202137445 90-91 20.336621186651154 28.59589643782566 29.31316963964817 21.75431273587501 92-93 20.434578505743538 28.86158852056029 29.308718387992727 21.395114585703443 94-95 20.16640385155912 28.954458545522865 29.252684704656463 21.626452898261554 96-97 19.87071788310077 29.19754166668732 29.114899865601725 21.816840584610183 98-99 20.148991799353347 29.25878885045197 28.569948412568575 22.022270937626107 100-101 20.432012911118093 28.56113995338753 29.290661744368983 21.71618539112539 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1554.0 1 956.0 2 456.5 3 552.5 4 512.5 5 510.0 6 810.5 7 1583.5 8 2463.0 9 2880.0 10 2649.0 11 2367.0 12 2511.5 13 2771.0 14 2966.0 15 3094.0 16 3175.0 17 3260.5 18 3341.0 19 3582.0 20 3998.5 21 4414.0 22 4978.5 23 5691.5 24 6442.5 25 7630.5 26 9371.0 27 11202.5 28 12565.5 29 13969.0 30 15761.5 31 17828.5 32 20175.5 33 22491.0 34 25079.5 35 27589.5 36 30516.0 37 33039.0 38 33373.5 39 33107.5 40 33046.5 41 32983.0 42 32984.5 43 33929.0 44 35393.5 45 35624.5 46 35735.5 47 38067.0 48 45037.0 49 44024.0 50 35006.0 51 29630.0 52 26440.5 53 24767.0 54 22939.5 55 20494.5 56 18108.0 57 15880.0 58 14062.5 59 12576.5 60 10678.0 61 8931.0 62 7035.0 63 4740.0 64 3047.5 65 2199.5 66 1612.5 67 1209.0 68 917.0 69 674.5 70 496.5 71 382.0 72 320.0 73 288.5 74 229.0 75 158.5 76 105.5 77 73.5 78 55.0 79 39.5 80 28.5 81 16.5 82 9.5 83 6.5 84 4.0 85 5.5 86 6.5 87 4.5 88 2.5 89 1.0 90 0.5 91 1.0 92 1.5 93 1.5 94 2.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3148077406483001 2 0.2689869346375604 3 0.2737372557790626 4 0.2680962494235288 5 0.2669086691381532 6 0.2045607041559372 7 0.1970393623485588 8 0.2046596691797185 9 0.20238347363274872 10-11 0.2060451795126566 12-13 0.20817292752395447 14-15 0.2039174315013588 16-17 0.1959012645750739 18-19 0.18922112546983647 20-21 0.18966646807685228 22-23 0.19609919462263647 24-25 0.19486213182537027 26-27 0.20114641083548251 28-29 0.20525345932240627 30-31 0.20762861989315737 32-33 0.20703482975046958 34-35 0.20688638221479766 36-37 0.19372403405188537 38-39 0.1861532097326163 40-41 0.18214512626947382 42-43 0.19120042594546235 44-45 0.19392196409944798 46-47 0.20084951576413865 48-49 0.20654000463156313 50-51 0.20322467633488972 52-53 0.2117356683800811 54-55 0.2014433059068264 56-57 0.17729584010419036 58-59 0.16250056904888674 60-61 0.17912669304414433 62-63 0.17145690370109395 64-65 0.1796215181630508 66-67 0.17957203565116017 68-69 0.1766030849377213 70-71 0.1796215181630508 72-73 0.18808302769635155 74-75 0.19214059367138467 76-77 0.19169525106436883 78-79 0.18862733532714868 80-81 0.18788509764878897 82-83 0.17749377015175297 84-85 0.1727434490102508 86-87 0.17892876299658175 88-89 0.1850151119591314 90-91 0.1827389164121616 92-93 0.17838445536578462 94-95 0.180858580960317 96-97 0.186895447410976 98-99 0.1859552796850537 100-101 0.16972501578492127 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1010458.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.97270973180634 #Duplication Level Percentage of deduplicated Percentage of total 1 80.430329827404 48.236248243723445 2 11.863744921078037 14.230018609680094 3 3.4699259404424727 6.243025836510646 4 1.4740798947159617 3.5361826258916804 5 0.7251496218484939 2.174459389162443 6 0.4282909558428863 1.5411461505313984 7 0.28062487881530607 1.1780884080499576 8 0.20097691449741448 0.9642504124758 9 0.1432087407438558 0.7729754615719895 >10 0.8771654243651049 9.772692146978413 >50 0.06985309896577134 2.8661897403112273 >100 0.031532019622178706 3.452522128151825 >500 0.002806514457897406 1.1937442571087402 >1k 0.0019810690291040514 2.2421441856585 >5k 3.3017817151734187E-4 1.5963124041937646 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9742 0.964117261677378 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6381 0.6314958167484448 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4559 0.45118154341892486 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3774 0.37349399975060815 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1780 0.1761577423307055 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1661 0.16438090450073137 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1657 0.16398504440560616 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1587 0.15705749274091552 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1375 0.13607690769928094 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1356 0.1341965722474363 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1331 0.13172244665290392 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1280 0.12667523044005785 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1268 0.12548765015468233 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1018 0.10074639420935853 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 9.896502378129521E-5 0.0 16-17 0.0 0.0 0.0 9.896502378129521E-5 0.0 18-19 0.0 0.0 0.0 9.896502378129521E-5 0.0 20-21 0.0 0.0 0.0 2.4741255945323804E-4 0.0 22-23 0.0 0.0 0.0 7.917201902503618E-4 0.0 24-25 0.0 0.0 0.0 0.0013360278210474853 0.0 26-27 0.0 0.0 0.0 0.002127748011297847 0.0 28-29 0.0 0.0 0.0 0.003958600951251808 0.0 30-31 0.0 0.0 0.0 0.00786771939061297 0.0 32-33 0.0 0.0 0.0 0.012222180436989959 0.0 34-35 0.0 0.0 0.0 0.015586991245553995 0.0 36-37 0.0 0.0 0.0 0.021574375184322356 0.0 38-39 0.0 0.0 0.0 0.029936919693841803 0.0 40-41 0.0 0.0 0.0 0.04027876467898715 0.0 42-43 0.0 0.0 0.0 0.05655851109101022 0.0 44-45 0.0 0.0 0.0 0.06902810408745341 0.0 46-47 0.0 0.0 0.0 0.09594659055596572 0.0 48-49 0.0 0.0 0.0 0.1302379712961845 0.0 50-51 0.0 0.0 0.0 0.14824960562438022 0.0 52-53 0.0 0.0 0.0 0.18234305631703646 0.0 54-55 0.0 0.0 0.0 0.22766903720886966 0.0 56-57 0.0 0.0 0.0 0.3136201603629245 0.0 58-59 0.0 0.0 0.0 0.425054777140663 0.0 60-61 0.0 0.0 0.0 0.5561834336508791 0.0 62-63 0.0 0.0 0.0 0.703344424013665 0.0 64-65 0.0 0.0 0.0 0.8666861957646929 0.0 66-67 0.0 0.0 0.0 1.075749808502679 0.0 68-69 0.0 0.0 0.0 1.337215401332861 0.0 70-71 0.0 0.0 0.0 1.4963511595731838 0.0 72-73 0.0 0.0 0.0 1.6837909146149568 0.0 74-75 0.0 0.0 0.0 1.9100249589789975 0.0 76-77 0.0 0.0 0.0 2.1657505804298642 0.0 78-79 0.0 0.0 0.0 2.438102325875989 0.0 80-81 0.0 0.0 0.0 2.823521610992243 0.0 82-83 0.0 0.0 0.0 3.2377397180288545 0.0 84-85 0.0 0.0 0.0 3.7606214211773272 0.0 86-87 0.0 0.0 0.0 4.444172840434733 0.0 88-89 0.0 0.0 0.0 5.176217121344974 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACCG 65 0.0058212234 29.253864 5 TACCTGG 780 0.0 26.196184 2 TACATGG 9160 0.0 25.730627 2 GTACATG 9235 0.0 25.636023 1 ACATGGG 9280 0.0 24.168972 3 GTATCAA 14505 0.0 23.860075 1 CATGGGA 6740 0.0 22.780209 4 TGGGCGA 105 0.00219419 22.62232 6 GAGTACT 7320 0.0 22.322311 12-13 AGTACTT 7485 0.0 22.210995 12-13 AGTATCG 130 2.9254932E-4 21.9404 5 ATGGGAG 2200 0.0 21.824047 5 ATGGGAT 2530 0.0 21.795864 5 GTACTTT 7665 0.0 21.25509 14-15 CATGGGG 2120 0.0 21.076916 4 TATCAAC 16870 0.0 20.421473 2 TCAACGC 16860 0.0 20.384298 4 ATCAACG 16885 0.0 20.37519 3 CAACGCA 16985 0.0 20.207298 5 GTATAGG 330 2.7284841E-11 20.16844 1 >>END_MODULE