FastQCFastQC Report
Thu 26 May 2016
SRR937624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937624_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010458
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128131.2680388497097355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86680.8578288261362669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67210.6651439248340851No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35530.3516227294949419No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22910.22672886948294732No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20290.200800033252248No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18210.18021530830573856No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17070.16893329559467093No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16640.16467779957207523No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16240.16071919862082343No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12670.12538868513090104No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT11880.11757044825217872No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.11677872806192835No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10410.1030225897563283No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT350.00839292227.14294224-25
GTATCAA154000.026.4157521
TACATGG83850.025.211282
GTACATG84300.025.143011
ACATGGG84450.023.682113
AGTACTT99000.022.81333512-13
GAGTACT97300.022.47968712-13
TATCAAC182000.022.2385332
ATCAACG183150.022.0210863
CATGGGG19300.021.9063824
TCAACGC184300.021.8836774
CAACGCA185550.021.7106515
CATGGGA64150.021.697464
ATGGGAG19450.021.4931965
GTACTTT101500.021.3623114-15
AACGCAG188600.021.356386
ACTATAC2751.2569217E-920.729393
TACTTTT109050.020.40597714-15
CATTCCG2108.609404E-720.35629
GGTATCA111900.019.833381
Produced by FastQC (version 0.11.5)