##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937624_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1010458 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24664657016917 33.0 31.0 34.0 31.0 34.0 2 32.44432722587183 34.0 31.0 34.0 31.0 34.0 3 32.54877293267013 34.0 31.0 34.0 31.0 34.0 4 36.16620482988902 37.0 35.0 37.0 35.0 37.0 5 36.027438052843365 37.0 35.0 37.0 35.0 37.0 6 36.142260242385134 37.0 35.0 37.0 35.0 37.0 7 36.10662194767126 37.0 35.0 37.0 35.0 37.0 8 36.11773473019166 37.0 35.0 37.0 35.0 37.0 9 37.76081539262394 39.0 38.0 39.0 35.0 39.0 10-11 37.728541908718626 39.0 37.0 39.0 35.0 39.0 12-13 37.64789184706341 39.0 37.0 39.0 35.0 39.0 14-15 38.80089820655584 40.0 38.0 41.0 35.0 41.0 16-17 38.8137215995123 40.0 38.0 41.0 35.5 41.0 18-19 38.90053569767373 40.0 38.0 41.0 35.0 41.0 20-21 38.86955321250363 40.0 38.0 41.0 35.0 41.0 22-23 38.72575010539775 40.0 38.0 41.0 35.0 41.0 24-25 38.659151097819006 40.0 38.0 41.0 34.5 41.0 26-27 38.546470016566744 40.0 38.0 41.0 34.0 41.0 28-29 38.41357038095596 40.0 38.0 41.0 34.0 41.0 30-31 38.356167203386974 40.0 37.5 41.0 34.0 41.0 32-33 38.15323546352248 40.0 37.0 41.0 34.0 41.0 34-35 37.96343588748864 40.0 37.0 41.0 34.0 41.0 36-37 37.745423362475236 40.0 36.5 41.0 33.0 41.0 38-39 37.55989808581851 40.0 36.0 41.0 33.0 41.0 40-41 37.30267710285831 40.0 36.0 41.0 33.0 41.0 42-43 36.970377294256664 40.0 36.0 41.0 32.5 41.0 44-45 36.464242947257574 40.0 36.0 41.0 31.0 41.0 46-47 35.804152671362885 40.0 35.0 41.0 29.0 41.0 48-49 35.23165040011559 39.0 35.0 41.0 25.0 41.0 50-51 34.88810074243561 39.0 35.0 41.0 23.0 41.0 52-53 34.64302969544504 39.0 35.0 40.5 22.0 41.0 54-55 34.44548610630031 39.0 35.0 40.0 20.0 41.0 56-57 34.18274683361406 38.5 34.5 40.0 18.5 41.0 58-59 33.953889226469585 38.0 34.0 40.0 17.0 41.0 60-61 33.61667778373767 38.0 34.0 40.0 12.5 41.0 62-63 33.27829063652324 37.5 33.5 40.0 8.0 41.0 64-65 32.89595361707266 37.0 33.0 40.0 7.0 41.0 66-67 32.482911214518566 36.5 32.5 39.0 2.0 41.0 68-69 32.03597032236867 36.0 32.0 39.0 2.0 40.0 70-71 31.629467528586048 35.5 31.5 38.5 2.0 40.0 72-73 31.18730466778431 35.0 31.0 38.0 2.0 40.0 74-75 30.717014462748573 35.0 31.0 37.0 2.0 39.0 76-77 29.480910636562825 33.5 29.5 36.0 2.0 38.5 78-79 30.008989982760294 34.0 30.0 36.0 2.0 39.0 80-81 29.795687698053754 34.0 30.0 36.0 2.0 37.5 82-83 29.44733031951848 34.0 30.0 35.5 2.0 37.0 84-85 29.053443092142373 34.0 29.5 35.0 2.0 37.0 86-87 28.658762165275547 34.0 29.0 35.0 2.0 36.0 88-89 28.237299818498148 34.0 29.0 35.0 2.0 36.0 90-91 27.75345288967973 34.0 27.5 35.0 2.0 35.0 92-93 27.366086962545694 33.5 26.5 35.0 2.0 35.0 94-95 26.952988149928053 33.0 26.0 35.0 2.0 35.0 96-97 26.470581162205654 33.0 25.0 35.0 2.0 35.0 98-99 25.765215872406372 33.0 24.0 35.0 2.0 35.0 100-101 23.519537180169785 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2567436692112963 1101 2 0.12070234131026325 1101 3 0.16821467190314365 1101 4 0.0992480161553857 1101 5 -0.03847674200657991 1101 6 0.02573294243950386 1101 7 0.11893546749709571 1101 8 0.07453957923701182 1101 9 0.2797372722876119 1101 10-11 0.2596305781348036 1101 12-13 0.21205353224653578 1101 14-15 0.25880816205230417 1101 16-17 0.054582819728828724 1101 18-19 0.10542284586092165 1101 20-21 0.23565979697871597 1101 22-23 0.20378666807814483 1101 24-25 0.2214112830100774 1101 26-27 0.246147039620503 1101 28-29 0.18795669147060323 1101 30-31 0.1880648524278854 1101 32-33 0.20951268628424202 1101 34-35 0.22096613233259887 1101 36-37 0.18179907048734378 1101 38-39 0.32948210811532874 1101 40-41 0.23495726206224532 1101 42-43 0.21211755930983145 1101 44-45 0.14872194262749616 1101 46-47 0.30751210939854445 1101 48-49 0.548546227017745 1101 50-51 0.5237145066888047 1101 52-53 0.756970279403717 1101 54-55 0.6906052351206071 1101 56-57 0.8183765263587404 1101 58-59 0.6676009916310122 1101 60-61 0.8036111268053574 1101 62-63 0.7919115605651541 1101 64-65 0.72504073226591 1101 66-67 0.9348547266861473 1101 68-69 0.8172075241260863 1101 70-71 0.7723613650515055 1101 72-73 0.7771742899536029 1101 74-75 0.8806304929551416 1101 76-77 0.5844767009502085 1101 78-79 0.7052560862519464 1101 80-81 0.7301404244952039 1101 82-83 0.7217573600297484 1101 84-85 0.8463415171270512 1101 86-87 1.15637444886152 1101 88-89 1.2542077307806245 1101 90-91 1.307153508509824 1101 92-93 1.2880838981344631 1101 94-95 1.0257563189794503 1101 96-97 1.132576185075738 1101 98-99 1.5713542010039312 1101 100-101 2.0319482250559453 1102 1 0.13689321803011723 1102 2 0.2019038013122909 1102 3 0.18617523157058713 1102 4 0.1500849825956152 1102 5 -0.016557447674223624 1102 6 0.055298997392355886 1102 7 0.06669629077601513 1102 8 0.04494310757524289 1102 9 0.1398057098826655 1102 10-11 0.14335155552505086 1102 12-13 0.1407200130333166 1102 14-15 0.21190027398579048 1102 16-17 0.27181388532753914 1102 18-19 0.22936510480850103 1102 20-21 0.1734322799960495 1102 22-23 0.20901732812072993 1102 24-25 0.16925727307395277 1102 26-27 0.15260121177907138 1102 28-29 0.16752173253316016 1102 30-31 0.30959836150220355 1102 32-33 0.41900193904709226 1102 34-35 0.3752128118418696 1102 36-37 0.5184641318277059 1102 38-39 0.3429660886486374 1102 40-41 0.5701501546912624 1102 42-43 0.5335185022278139 1102 44-45 0.7423024962116003 1102 46-47 0.8533303188682808 1102 48-49 0.8253316878308894 1102 50-51 0.7905544641054121 1102 52-53 0.5958162187730807 1102 54-55 0.6655175336099077 1102 56-57 0.7638550825789494 1102 58-59 0.8598923837023804 1102 60-61 0.6541515303336922 1102 62-63 0.670532416288566 1102 64-65 0.6978388160132454 1102 66-67 0.8484976345235253 1102 68-69 0.7591595164205209 1102 70-71 0.8333468664202606 1102 72-73 0.7100765509289637 1102 74-75 0.6341154873787431 1102 76-77 0.7077586638418225 1102 78-79 0.7080402290076968 1102 80-81 0.7974640075835104 1102 82-83 0.7847138641360019 1102 84-85 1.0186060411055564 1102 86-87 0.9368225883728236 1102 88-89 1.1606869967240527 1102 90-91 1.3054810964648063 1102 92-93 1.4494006881577803 1102 94-95 1.3373405225686206 1102 96-97 1.610513425266351 1102 98-99 1.5568000592620663 1102 100-101 2.247565937719436 1103 1 0.24883139681040234 1103 2 0.23205417725213806 1103 3 0.15870655989139237 1103 4 0.13864387983872462 1103 5 -0.024899135226434055 1103 6 0.03914193807739963 1103 7 0.11418167089297526 1103 8 0.10021879679830192 1103 9 0.22523177504557168 1103 10-11 0.22065105427191867 1103 12-13 0.16071792447859679 1103 14-15 0.270137533802 1103 16-17 0.17646091957983145 1103 18-19 0.17017964114935324 1103 20-21 0.08910688317065052 1103 22-23 0.17265767899791484 1103 24-25 0.20450581108564592 1103 26-27 0.20488400208817126 1103 28-29 0.15400878349389302 1103 30-31 0.24666811922985943 1103 32-33 0.27982482960682376 1103 34-35 0.23507914433978527 1103 36-37 0.1545146748519315 1103 38-39 0.24147694913317252 1103 40-41 0.21884063088173633 1103 42-43 0.1941200059872088 1103 44-45 0.13670308100692097 1103 46-47 0.5373257240478964 1103 48-49 0.43930203035386484 1103 50-51 0.5196626512399121 1103 52-53 0.4858329272108435 1103 54-55 0.6256658209950459 1103 56-57 0.63391982777528 1103 58-59 0.4694224588903566 1103 60-61 0.5112150223971881 1103 62-63 0.761927570841614 1103 64-65 0.7696809212764038 1103 66-67 0.8864550280072336 1103 68-69 0.7292075531790836 1103 70-71 0.7725699240643671 1103 72-73 0.7986646879381567 1103 74-75 0.8611810679969558 1103 76-77 0.823583642956276 1103 78-79 0.9666116575791257 1103 80-81 0.8796590785943756 1103 82-83 0.903669586775937 1103 84-85 0.9600910035115717 1103 86-87 1.0197778933018142 1103 88-89 1.0076523267156965 1103 90-91 0.9452304698983909 1103 92-93 1.189244046553771 1103 94-95 1.0410205559855008 1103 96-97 1.3898534419747897 1103 98-99 1.5107704017850416 1103 100-101 2.2260642668756603 1104 1 0.02019920611664361 1104 2 0.15863177706733467 1104 3 0.053858897575395304 1104 4 0.07301162069781952 1104 5 6.888685552652873E-4 1104 6 -0.03461948938787174 1104 7 0.05507595427272349 1104 8 0.006729083121278734 1104 9 0.08767225811131851 1104 10-11 0.06844090655441448 1104 12-13 0.08229567402192828 1104 14-15 0.1651981630024153 1104 16-17 -0.006078266412458788 1104 18-19 -0.04666974731815543 1104 20-21 0.006774322006712907 1104 22-23 0.07638382409731292 1104 24-25 -0.016510832266213527 1104 26-27 -0.022079789370472724 1104 28-29 -0.06796264409971542 1104 30-31 0.12382607599692363 1104 32-33 0.1359914363822341 1104 34-35 0.25125994320355716 1104 36-37 0.3343558132991191 1104 38-39 0.052912007762436986 1104 40-41 0.08164848262521929 1104 42-43 0.1678499370694837 1104 44-45 0.09060900681877371 1104 46-47 0.24924263990496343 1104 48-49 0.4126025625304379 1104 50-51 0.4949564392334267 1104 52-53 0.3526494824369948 1104 54-55 0.22359256757375334 1104 56-57 0.35492363728686627 1104 58-59 0.21464105127475364 1104 60-61 0.24450161916220736 1104 62-63 0.3971221853344389 1104 64-65 0.3881332563936368 1104 66-67 0.33497689045413637 1104 68-69 0.4015184358478372 1104 70-71 0.5148182845412741 1104 72-73 0.5709768539909383 1104 74-75 0.7290347579399317 1104 76-77 0.3192551629535352 1104 78-79 0.5238537936435002 1104 80-81 0.6005481856585462 1104 82-83 0.7304009787770305 1104 84-85 0.7840115171218613 1104 86-87 0.7966532916856046 1104 88-89 0.7589611110515655 1104 90-91 0.8420768744980371 1104 92-93 1.001521860694094 1104 94-95 1.0858758972159208 1104 96-97 1.1972116173449372 1104 98-99 1.411329529426137 1104 100-101 1.9535788439792334 1105 1 0.12620421676589189 1105 2 0.1341035484677917 1105 3 0.12705386621028225 1105 4 0.06236690421381752 1105 5 -0.04440826941885234 1105 6 -0.07854423901725482 1105 7 0.07541310493530773 1105 8 0.05640960567890829 1105 9 0.16466664540731557 1105 10-11 0.06979909029117692 1105 12-13 -0.013941807447089616 1105 14-15 0.0642928150730242 1105 16-17 0.12389985245774682 1105 18-19 0.11656801252026128 1105 20-21 0.048337463308314454 1105 22-23 0.06353060245700703 1105 24-25 -0.05158343489065231 1105 26-27 0.029431173852394465 1105 28-29 0.04647242785554084 1105 30-31 -0.0968428521514042 1105 32-33 9.583233707317618E-4 1105 34-35 -0.025665822797826365 1105 36-37 0.056811160904821634 1105 38-39 -0.24644288733114195 1105 40-41 -0.2757758882923014 1105 42-43 -0.317704633043995 1105 44-45 -0.25623732679724753 1105 46-47 -0.0942486950381678 1105 48-49 -0.1793396143182875 1105 50-51 -0.1135827040361832 1105 52-53 -0.19841576605625022 1105 54-55 -0.2853294954672023 1105 56-57 -0.1446594559798271 1105 58-59 -0.13642872881342072 1105 60-61 -0.10616452529209397 1105 62-63 -0.07256176828791894 1105 64-65 -0.09182932557967405 1105 66-67 0.06547614274096958 1105 68-69 -0.2042823293443341 1105 70-71 -0.13713353813094287 1105 72-73 0.2178451982108882 1105 74-75 0.17554089825105734 1105 76-77 0.1888458951945431 1105 78-79 0.30687714430478863 1105 80-81 0.392391314789581 1105 82-83 0.3990248628717801 1105 84-85 0.4563241194873555 1105 86-87 0.4050021079682722 1105 88-89 0.5565972369263257 1105 90-91 0.6477883025330762 1105 92-93 1.0275960105345199 1105 94-95 0.9759814833777263 1105 96-97 0.8414047021310793 1105 98-99 1.202637606670411 1105 100-101 1.6649647999191828 1106 1 -0.06133273409994899 1106 2 0.02197154179098959 1106 3 -0.204739264891856 1106 4 -0.02403890721320323 1106 5 -0.1617614217823089 1106 6 -0.1890499413401443 1106 7 -0.2611171971059676 1106 8 -0.20787790702659237 1106 9 -0.1450287022805128 1106 10-11 -0.12061653118275473 1106 12-13 -0.18717212907115055 1106 14-15 -0.17658346829931304 1106 16-17 -0.2835150323301505 1106 18-19 -0.2202047431517542 1106 20-21 -0.041944808668709754 1106 22-23 -0.055545369455678895 1106 24-25 -0.22738656254248468 1106 26-27 -0.25110306339781374 1106 28-29 -0.14181892680749542 1106 30-31 -0.24659387092290785 1106 32-33 -0.38232916135935113 1106 34-35 -0.40672832920885327 1106 36-37 -0.27367458561922575 1106 38-39 -0.36432617388335586 1106 40-41 -0.4649932293219834 1106 42-43 -0.4036525801145032 1106 44-45 -0.09189666079577563 1106 46-47 -0.005047673002863462 1106 48-49 -0.00825780486819383 1106 50-51 -0.0667980121854228 1106 52-53 -0.011934420998109374 1106 54-55 -0.19119780584515667 1106 56-57 -0.27491580406864813 1106 58-59 -0.12900871519871515 1106 60-61 -0.1497292464237887 1106 62-63 0.07470410076847145 1106 64-65 -0.024524197685387605 1106 66-67 0.06739572273668415 1106 68-69 -0.2549639970523039 1106 70-71 -0.006334000657869154 1106 72-73 -0.04532236522337385 1106 74-75 -0.11493953294339931 1106 76-77 -0.22461074756868626 1106 78-79 -0.050511630092106685 1106 80-81 -0.020955757582829193 1106 82-83 -0.014223363020402502 1106 84-85 -0.059482924723134545 1106 86-87 0.08959091593567337 1106 88-89 0.23461368647265957 1106 90-91 0.20751255815380532 1106 92-93 0.590508997656503 1106 94-95 0.1795119812469288 1106 96-97 0.2707429902219438 1106 98-99 0.4222068784702522 1106 100-101 1.1245962704004526 1107 1 -0.018153467310689564 1107 2 0.043830349709431005 1107 3 -0.06029068813062821 1107 4 -0.020053517248769026 1107 5 -0.16927764686501234 1107 6 -0.08105643366895521 1107 7 -0.034926802905900445 1107 8 -0.13529031912877088 1107 9 0.05558379889605192 1107 10-11 -0.11221644945471354 1107 12-13 -0.12408313044630148 1107 14-15 0.03707223165775275 1107 16-17 -0.15085962852244705 1107 18-19 -0.20335120393721695 1107 20-21 -0.074700306371831 1107 22-23 0.07531362441702072 1107 24-25 0.07009605303037603 1107 26-27 -0.0023886730637556752 1107 28-29 -0.12782529516680796 1107 30-31 -0.1071258837514577 1107 32-33 -0.1271597478498876 1107 34-35 0.051656945050716274 1107 36-37 -0.03636079814427973 1107 38-39 0.017377075265500252 1107 40-41 -0.04995021879145867 1107 42-43 -0.202974650588871 1107 44-45 -0.27644478047685084 1107 46-47 -0.3235775553925393 1107 48-49 -0.18344048770599386 1107 50-51 -0.17208714809503078 1107 52-53 -0.2345331431939357 1107 54-55 -0.20424903968607566 1107 56-57 0.03914141933588411 1107 58-59 -0.06846740465740453 1107 60-61 -0.12576754840891624 1107 62-63 -0.2555374561048396 1107 64-65 -0.06805987679721426 1107 66-67 -0.15850827895297215 1107 68-69 0.0811024046869413 1107 70-71 0.20675178394282412 1107 72-73 0.18682258267499563 1107 74-75 0.04984922531836489 1107 76-77 -0.1994713392705343 1107 78-79 0.1158491303460103 1107 80-81 0.14592728178208247 1107 82-83 0.1730767648518423 1107 84-85 0.15857024901094263 1107 86-87 0.25060721333392166 1107 88-89 0.4238818302130021 1107 90-91 0.61087947803966 1107 92-93 0.5647313759884689 1107 94-95 0.5560131027117912 1107 96-97 0.6691362077126648 1107 98-99 0.6790137225051431 1107 100-101 1.393375928382369 1108 1 0.0847936509305498 1108 2 0.09259644200493256 1108 3 0.13758215797751205 1108 4 0.09309364060427328 1108 5 -0.07157909269587037 1108 6 -0.004138232044873291 1108 7 0.007453866533587927 1108 8 -0.050857758225625105 1108 9 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-0.3647870301191425 2316 100-101 -0.7633679813027179 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 9.0 5 15.0 6 45.0 7 101.0 8 253.0 9 520.0 10 951.0 11 1877.0 12 3346.0 13 4989.0 14 6638.0 15 9710.0 16 14136.0 17 21121.0 18 18092.0 19 8633.0 20 5996.0 21 5652.0 22 5517.0 23 5780.0 24 6347.0 25 7201.0 26 8441.0 27 10241.0 28 12498.0 29 15549.0 30 19587.0 31 24634.0 32 31801.0 33 42696.0 34 60553.0 35 88779.0 36 138628.0 37 221419.0 38 191035.0 39 17663.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.658965684192395 19.955920835721944 15.457410438207486 19.927703041878175 2 14.975486363609372 21.894230141183503 41.12234254169891 22.00794095350821 3 20.145914031063143 27.52919963026667 30.00708589570274 22.317800442967446 4 12.126580224017228 17.20170457356961 38.34063365325427 32.331081549158895 5 12.163874196306972 39.46330725619992 33.14531007506414 15.227508472428974 6 29.113035870862518 37.44975050917505 17.716025802161 15.721187817801432 7 26.51144332569983 35.76041755322834 20.375215991164403 17.352923129907428 8 26.402185939445282 33.582296344825814 19.92274790243632 20.092769813292584 9 27.63271704514191 17.186958785026196 21.246009235416018 33.93431493441588 10-11 24.36726712045429 28.250209311025298 26.192330606517046 21.19019296200337 12-13 26.577963269311372 25.951286039260356 27.82909090729146 19.641659784136813 14-15 23.06746049811076 25.925916762497796 29.10393108867464 21.902691650716804 16-17 19.66578521818819 29.753339574727498 29.22907236124609 21.351802845838225 18-19 19.461620374127374 27.081283932632527 34.32062490474617 19.13647078849393 20-21 20.911171424824893 25.513987475970048 34.43311569264417 19.141725406560887 22-23 20.17205120465533 25.79273693534101 34.37441548609289 19.660796373910763 24-25 19.937780258518227 25.877009375021647 34.893280327299394 19.291930039160732 26-27 19.86740416789138 25.807644004239744 35.16441863388092 19.16053319398796 28-29 19.339711105052125 26.49597475942328 35.048268743260955 19.11604539226364 30-31 19.620091562802465 26.26081939405455 34.794161832206115 19.324927210936867 32-33 19.378885614246215 26.296689224094422 34.69990835838798 19.624516803271387 34-35 19.86232975541784 26.07822393409721 34.64107365175 19.41837265873495 36-37 19.98510575655087 25.900644015210933 34.64356491985066 19.470685308387537 38-39 19.7371390003345 26.43251871923425 34.597232146214886 19.233110134216368 40-41 19.710467926425444 26.56062894251913 34.37926168133658 19.349641449718842 42-43 19.47340994378678 26.795133763118606 33.79117820120661 19.940278091888004 44-45 19.882667067804896 26.718032812843283 33.26372793327382 20.135572186077997 46-47 20.31697507466911 27.277284162231386 32.34666854040445 20.059072222695054 48-49 20.42944882419655 27.51851140769829 31.71225325545446 20.339786512650697 50-51 20.524257316979032 27.94524859024324 31.439802544984552 20.09069154779318 52-53 20.4443239268588 28.644775900793356 30.578555336127778 20.332344836220066 54-55 20.15110969481166 28.221163076545487 30.90504503898232 20.722682189660528 56-57 20.68596616583767 28.40954299931318 30.241187659457395 20.663303175391754 58-59 20.81462069675335 27.914223055287803 30.585289047144958 20.685867200813888 60-61 20.5768077446069 28.022342343768862 31.198080474398736 20.202769437225495 62-63 20.64751825409864 27.97988634856669 31.102876121521135 20.269719275813543 64-65 20.340776162888513 28.179102941438437 31.164927191432 20.315193704241047 66-67 20.467055533233445 28.1671281735609 30.551492491523646 20.81432380168201 68-69 20.54167516116454 28.144712595674438 30.64585564169911 20.66775660146191 70-71 20.6768119011379 28.186376870686363 30.409675612445046 20.72713561573069 72-73 20.520259387455685 27.90597328437861 30.58718451790402 20.986582810261687 74-75 20.355433058787728 28.71659619528778 29.938072605725623 20.98989814019887 76-77 20.273776841788575 28.323591876159128 30.359055002780917 21.04357627927138 78-79 20.216228680459754 28.186624283245816 30.330899453515137 21.266247582779293 80-81 20.192971899871146 28.517167462675342 30.043999849573165 21.245860787880346 82-83 20.240614138567143 28.283369092766037 30.319430039506916 21.156586729159905 84-85 20.131138682794024 28.11514987772859 30.026710686352807 21.727000753124575 86-87 19.961789604318042 28.184199640163172 29.98694651336325 21.867064242155536 88-89 19.983413462014255 27.86686829140845 30.06824628039958 22.081471966177713 90-91 19.87845293478116 27.738483289381684 30.249938019013438 22.133125756823723 92-93 19.746098824018425 27.840108072533297 30.456611401553296 21.95718170189498 94-95 19.425696070494766 28.003786401809872 30.40744889940997 22.16306862828539 96-97 19.243155084130166 28.08008843514525 30.410120955052065 22.26663552567252 98-99 19.389187570976514 28.108703235910465 29.90640378244508 22.59570541066794 100-101 19.033936679574897 27.875091244262435 30.467294344711544 22.623677731451124 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 667.0 1 495.0 2 479.5 3 675.0 4 565.5 5 515.5 6 898.0 7 1874.5 8 3004.5 9 3364.0 10 3069.5 11 2857.0 12 2979.0 13 3145.5 14 3305.5 15 3529.0 16 3802.0 17 3969.0 18 4067.5 19 4288.5 20 4556.0 21 4951.0 22 5491.0 23 6121.0 24 6889.5 25 8032.5 26 9638.5 27 11456.0 28 12862.5 29 14265.5 30 15980.0 31 17687.5 32 19715.0 33 21837.0 34 24502.0 35 27179.0 36 29940.0 37 32263.0 38 32940.0 39 32952.5 40 32866.0 41 32904.0 42 32829.0 43 33546.0 44 35132.5 45 35636.5 46 35430.5 47 37888.0 48 45849.5 49 44146.5 50 34223.5 51 29449.5 52 26251.5 53 24418.5 54 22717.5 55 20288.5 56 17601.5 57 15480.5 58 13794.5 59 12150.5 60 10336.5 61 8592.5 62 6743.5 63 4473.5 64 2830.5 65 2037.5 66 1480.5 67 1134.0 68 864.5 69 623.0 70 484.0 71 397.5 72 303.0 73 287.0 74 251.5 75 184.0 76 136.0 77 83.0 78 52.0 79 31.5 80 18.0 81 12.5 82 12.0 83 6.0 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04522701586805192 2 0.0 3 0.0 4 0.0 5 0.012073732901318017 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004898768677174113 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.948251189064761E-5 22-23 2.9689507134388564E-4 24-25 6.927551664690665E-4 26-27 0.001880335451844609 28-29 0.002127748011297847 30-31 3.958600951251809E-4 32-33 0.0 34-35 0.0 36-37 4.948251189064761E-5 38-39 0.0 40-41 0.0 42-43 0.01073770508027053 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.4844753567194282E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.948251189064761E-5 74-75 4.948251189064761E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.9793004756259045E-4 84-85 9.896502378129522E-5 86-87 0.0 88-89 0.0 90-91 0.006680139105237426 92-93 0.002523608106423028 94-95 0.0 96-97 0.0 98-99 4.948251189064761E-5 100-101 0.011925285365646073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1010458.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.54338432316416 #Duplication Level Percentage of deduplicated Percentage of total 1 79.5492705623474 45.77534248596525 2 12.178876192309328 14.016275067165795 3 3.6708140590256693 6.3369319253216485 4 1.5705557307981832 3.615003680730712 5 0.8071945678094713 2.3224353619515394 6 0.4621863561081011 1.5957460271070762 7 0.31364875974721357 1.263388778723238 8 0.2199040678579465 1.012321943278159 9 0.16146667535261475 0.836220505967918 >10 0.9513679135129751 10.193942809818365 >50 0.07168092931658475 2.79615113676265 >100 0.03730786303298539 4.080456995336864 >500 0.0032969737226749714 1.4061175392188754 >1k 0.0019087742604960362 1.9336362836936176 >5k 3.4704986554473386E-4 1.5366242587018373 >10k+ 1.7352493277236693E-4 1.2794052002564587 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12813 1.2680388497097355 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8668 0.8578288261362669 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6721 0.6651439248340851 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3553 0.3516227294949419 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2291 0.22672886948294732 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2029 0.200800033252248 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1821 0.18021530830573856 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1707 0.16893329559467093 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1664 0.16467779957207523 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1624 0.16071919862082343 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1267 0.12538868513090104 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1188 0.11757044825217872 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1180 0.11677872806192835 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1041 0.1030225897563283 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 9.896502378129521E-5 0.0 16-17 0.0 0.0 0.0 9.896502378129521E-5 0.0 18-19 0.0 0.0 0.0 9.896502378129521E-5 0.0 20-21 0.0 0.0 0.0 2.9689507134388564E-4 0.0 22-23 0.0 0.0 0.0 8.90685214031657E-4 0.0 24-25 0.0 0.0 0.0 0.0013360278210474853 0.0 26-27 0.0 0.0 0.0 0.002028782987516552 0.0 28-29 0.0 0.0 0.0 0.003958600951251808 0.0 30-31 0.0 0.0 0.0 0.008016166926284912 0.0 32-33 0.0 0.0 0.0 0.012519075508333845 0.0 34-35 0.0 0.0 0.0 0.01598285134067918 0.0 36-37 0.0 0.0 0.0 0.022217647838900775 0.0 38-39 0.0 0.0 0.0 0.030481227324638926 0.0 40-41 0.0 0.0 0.0 0.041119967381128164 0.0 42-43 0.0 0.0 0.0 0.057647126352604466 0.0 44-45 0.0 0.0 0.0 0.06996827181337571 0.0 46-47 0.0 0.0 0.0 0.09713417084134125 0.0 48-49 0.0 9.896502378129521E-5 0.0 0.13206882423613847 0.0 50-51 0.0 9.896502378129521E-5 0.0 0.15008045856433422 0.0 52-53 0.0 9.896502378129521E-5 0.0 0.18476769939967816 0.0 54-55 0.0 9.896502378129521E-5 0.0 0.23073695294608979 0.0 56-57 0.0 9.896502378129521E-5 0.0 0.31757876131417634 0.0 58-59 0.0 9.896502378129521E-5 0.0 0.42995354581783707 0.0 60-61 0.0 9.896502378129521E-5 0.0 0.5624677126609914 0.0 62-63 0.0 9.896502378129521E-5 0.0 0.7101235281426839 0.0 64-65 0.0 9.896502378129521E-5 0.0 0.8753951178574468 0.0 66-67 0.0 9.896502378129521E-5 0.0 1.0871802687494185 0.0 68-69 0.0 9.896502378129521E-5 0.0 1.3516148122930396 0.0 70-71 0.0 9.896502378129521E-5 0.0 1.5126803884970974 0.0 72-73 0.0 9.896502378129521E-5 0.0 1.7013077238242462 0.0 74-75 0.0 9.896502378129521E-5 0.0 1.929075726056897 0.0 76-77 0.0 9.896502378129521E-5 0.0 2.187028060542843 0.0 78-79 0.0 9.896502378129521E-5 0.0 2.461656001535937 0.0 80-81 0.0 9.896502378129521E-5 0.0 2.850489579972646 0.0 82-83 0.0 9.896502378129521E-5 0.0 3.2671818126037895 0.0 84-85 0.0 9.896502378129521E-5 0.0 3.793824186655952 0.0 86-87 0.0 9.896502378129521E-5 0.0 4.490191576493036 0.0 88-89 0.0 9.896502378129521E-5 0.0 5.23475493291161 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGCGT 35 0.008392922 27.142942 24-25 GTATCAA 15400 0.0 26.415752 1 TACATGG 8385 0.0 25.21128 2 GTACATG 8430 0.0 25.14301 1 ACATGGG 8445 0.0 23.68211 3 AGTACTT 9900 0.0 22.813335 12-13 GAGTACT 9730 0.0 22.479687 12-13 TATCAAC 18200 0.0 22.238533 2 ATCAACG 18315 0.0 22.021086 3 CATGGGG 1930 0.0 21.906382 4 TCAACGC 18430 0.0 21.883677 4 CAACGCA 18555 0.0 21.710651 5 CATGGGA 6415 0.0 21.69746 4 ATGGGAG 1945 0.0 21.493196 5 GTACTTT 10150 0.0 21.36231 14-15 AACGCAG 18860 0.0 21.35638 6 ACTATAC 275 1.2569217E-9 20.72939 3 TACTTTT 10905 0.0 20.405977 14-15 CATTCCG 210 8.609404E-7 20.3562 9 GGTATCA 11190 0.0 19.83338 1 >>END_MODULE