FastQCFastQC Report
Thu 26 May 2016
SRR937623_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937623_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661972
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102730.6181211235809027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80300.4831609678141388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52600.31649149323815323No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21030.1265364278098548No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18530.11149405645823154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55450.050.086711
GTATCAA97150.039.3082731
ATCAACG119850.031.5380943
TCAACGC121100.031.0947724
TATCAAC123100.030.7054462
CAACGCA123150.030.578085
AACGCAG126850.029.6949736
GTACATG105000.026.3146761
ACGCAGA144500.025.904297
CGCAGAG145250.025.737838
TACATGG107550.025.419312
GTACCGA950.001226674825.0003816
ACATGGG108550.024.0025313
GAGTACT81000.023.54582212-13
GCAGAGT153550.023.5113249
TACCTGG14750.022.5638072
GTACCTG15600.022.558921
GTACTTT85850.022.32561514-15
CATGGGA68650.021.8160634
AGTACTT85400.020.96992112-13
Produced by FastQC (version 0.11.5)